BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0177.p (696 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313770|ref|YP_003777862.1| TonB-dependent siderophore rece... 1419 0.0 gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitr... 65 2e-11 gi|300310762|ref|YP_003774854.1| outer membrane ferric sideropho... 61 3e-10 gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore... 61 3e-10 gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor prote... 59 2e-09 >gi|300313770|ref|YP_003777862.1| TonB-dependent siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 696 Score = 1419 bits (3674), Expect = 0.0, Method: Compositional matrix adjust. Identities = 693/696 (99%), Positives = 693/696 (99%) Query: 1 MTSVATRRRRARRPDHLHRVRPLVRATALGGLLAFSAVPDALAQSTASSEDAALTLGTVE 60 MTSVA RRRRARRPDHLHRVRPLVRATALGGLLAFSAVPDALAQSTASSEDAALTLGTVE Sbjct: 1 MTSVAARRRRARRPDHLHRVRPLVRATALGGLLAFSAVPDALAQSTASSEDAALTLGTVE 60 Query: 61 VSGRRSGPLQLRNVLTSVDLIGADRLQQQQVLYNWELFAQSPGVMLTQFRQGNESGKLSF 120 VSGRRSGPLQLRNVLTSVDLIGADRLQQQQVLYNWELFAQSPGVMLTQFRQGNESGKLSF Sbjct: 61 VSGRRSGPLQLRNVLTSVDLIGADRLQQQQVLYNWELFAQSPGVMLTQFRQGNESGKLSF 120 Query: 121 RGFNGEGEVNAVKLLIDGVPSNDNAGGMPFLGTLFPLDMAGIEVVRGTNDARYGLHNIAG 180 RGFNGEGEVNAVKLLIDGVPSNDNAGGMPFLGTLFPLDMAGIEVVRGTNDARYGLHNIAG Sbjct: 121 RGFNGEGEVNAVKLLIDGVPSNDNAGGMPFLGTLFPLDMAGIEVVRGTNDARYGLHNIAG 180 Query: 181 NVNILTRQGGNYDEARLTYGSFNTRQVQVAKGVENGNWSQNYFLGYEKSDGYREHAQAEN 240 NVNILTRQGGNYDEARLTYGSFNTRQVQVAKGVENGNWSQNYFLGYEKSDGYREHAQAEN Sbjct: 181 NVNILTRQGGNYDEARLTYGSFNTRQVQVAKGVENGNWSQNYFLGYEKSDGYREHAQAEN 240 Query: 241 IAFSGKWFYTSDDGRYRTGLSIRHANAYAQEPGYLTAEDARANPRTSYAYAQSDGGNRQL 300 IAFSGKWFYTSDDGRYRTGLSIRHANAYAQEPGYLTAEDARANPRTSYAYAQSDGGNRQL Sbjct: 241 IAFSGKWFYTSDDGRYRTGLSIRHANAYAQEPGYLTAEDARANPRTSYAYAQSDGGNRQL 300 Query: 301 DQVSLHFDGDIDDQLAVGAKLYLNRVRDQRWLRYSSTTSQQERLIDETHYGLMTTATYRP 360 DQVSLHFDGDIDDQLAVGAKLYLNRVRDQRWLRYSSTTSQQERLIDETHYGLMTTATYRP Sbjct: 301 DQVSLHFDGDIDDQLAVGAKLYLNRVRDQRWLRYSSTTSQQERLIDETHYGLMTTATYRP 360 Query: 361 QWKALEGMNDLALEGGFNVERQEDQSPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVIKP 420 QWKALEGMNDLALEGGFNVERQEDQSPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVIKP Sbjct: 361 QWKALEGMNDLALEGGFNVERQEDQSPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVIKP 420 Query: 421 VESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWGRT 480 VESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWGRT Sbjct: 421 VESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWGRT 480 Query: 481 FQVGAGAAAYKSNNNDLRPSINDGWESGVKFTPVEWLDGRLAWWAQRASDEVKRRLNDPT 540 FQVGAGAAAYKSNNNDLRPSINDGWESGVKFTPVEWLDGRLAWW QRASDEVKRRLNDPT Sbjct: 481 FQVGAGAAAYKSNNNDLRPSINDGWESGVKFTPVEWLDGRLAWWEQRASDEVKRRLNDPT 540 Query: 541 GDSENVGRTKRSGVDLQLNLRAGKRTTMWLAYSRQRSEIITPDPTAPATIGKEIDHVPRY 600 GDSENVGRTKRSGVDLQLNLRAGKRTTMWLAYSRQRSEIITPDPTAPATIGKEIDHVPRY Sbjct: 541 GDSENVGRTKRSGVDLQLNLRAGKRTTMWLAYSRQRSEIITPDPTAPATIGKEIDHVPRY 600 Query: 601 LFSGGVDYQATDYLKLSTSLNGQGSYYLTTANTGGKFGGYTLVDASASYQVSPTLALELQ 660 LFSGGVDYQATDYLKLSTSLNGQGSYYLTTANTGGKFGGYTLVDASASYQVSPTLALELQ Sbjct: 601 LFSGGVDYQATDYLKLSTSLNGQGSYYLTTANTGGKFGGYTLVDASASYQVSPTLALELQ 660 Query: 661 VKNLANRYYEYVWINDQTRHAPGDGRAVYLSAKLTY 696 VKNLANRYYEYVWINDQTRHAPGDGRA YLSAKLTY Sbjct: 661 VKNLANRYYEYVWINDQTRHAPGDGRAAYLSAKLTY 696 >gi|300310609|ref|YP_003774701.1| outer membrane Iron(III) dicitrate transport protein [Herbaspirillum seropedicae SmR1] Length = 703 Score = 64.7 bits (156), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 150/717 (20%), Positives = 278/717 (38%), Gaps = 64/717 (8%) Query: 20 VRPLVRATALGGLLAFSAVPDALAQSTASSEDAALTLGTVEVSGRRSGPLQLRNVLT--- 76 VR L T LA A P A AS D L ++VSG G +V T Sbjct: 11 VRDLRITTTALASLAILAFPVAGLAQQASPSDGQPGLEPIQVSGDWLGTGLESSVKTYPG 70 Query: 77 SVDLIGADRLQQQQVLYNWELFAQSPGVMLTQFRQGNESGKLSF----RGFNGEGEVNAV 132 + ++ ++ + + + PGV + G ++ RG G + Sbjct: 71 ARTVVKKQEIESTGAVSIGDAMRRIPGVQSSD-NSGTAGSAIALNIGVRGLAGRYSPRST 129 Query: 133 KLLIDGVPSNDNAGGMPFLGTLFPLDMAGIE---VVRGTNDARYGLHNIAGNVNILTRQ- 188 +L+DG+P G P L + PL + IE VVRG R+G N+ G +N TR Sbjct: 130 -ILLDGIPMAVAPYGQPQL-SFAPLSLNNIESIDVVRGGGAVRFGPQNVGGIINFKTRSI 187 Query: 189 ---GGNYDEARLTYGSF-----NTRQVQVAKGVENGNWSQNYFLGYEKSDGYREHA--QA 238 G +A + Y S+ NT+ A G + + ++ G+R+ + Sbjct: 188 PTTPGISGDAAVRYNSYGQGGGNTQYTAFAGGQAENGFGMAFLYSGQEGSGWRDKSDEHL 247 Query: 239 ENIAFSGKWFYTSDDGRYRTGLSIRHANAYAQEPGYLTAEDARANPRTSYAYAQSDGGNR 298 + A ++ + Y I + + ++ PG L+ A+P S + GG R Sbjct: 248 NDFALKMRYEISPTSELY---AKINYYDVLSRTPGGLSVAQYAADPFQSTRQRDNWGGTR 304 Query: 299 QLDQVSLHFDGDIDDQLAVGAKLYLNRVRDQRWLRYSSTTSQQERLIDETHYGLMTTATY 358 + + + I D+ + Y ++ Q L + T + + G+ T Sbjct: 305 K--GFDVGYLNTISDRQEFEIRTYFSQATRQSTLINPAGTRLSHQPRNYETLGIEPRYTQ 362 Query: 359 RPQWKALEGMNDLALEGGFNVERQEDQSPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVI 418 R W +D+ + + ER +D + V T+ + T D Y+ I Sbjct: 363 RFAWGGT--THDVTVGYRYVRERGDDNVYSEAVLTGVLSGFTQFQNST-DAHAGYIDDKI 419 Query: 419 KPVESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWG 478 ++I PG R + V + D + Y + + + P ++V Y +Q +L++N+ Sbjct: 420 A-FGKWRITPGLRFEHVQSTRYDVANNASYQVSNNKPL--PSINVAYLLTEQLTLFSNYN 476 Query: 479 RTFQVGAGAAA-YKSNNNDLRPSINDGWESGVKFTPVEWLDGRLAWWAQRASDEVKRRLN 537 +F V + S+ N L P + E GV+ W R + A + ++ Sbjct: 477 TSFGVVQNSQLNTMSSANPLSPELAKTIEGGVR-----WKSARASAEATVFHIDFDNQIV 531 Query: 538 DPTGDS----ENVGRTKRSGVDLQLNLRAGKRTTM--WLAYSR-QRSEIITPDPTAPATI 590 G + N+G+T+ G++L ++ +++ + W Y+ + + P Sbjct: 532 SVQGTNPSVFRNIGKTQHDGIELAMDYAFDQQSVLKGWSVYANYTYTRALQKSGANP--- 588 Query: 591 GKEIDHVPRYLFSGGVDYQATDY-LKLSTSLNGQGSYYLTTANT--------GGKFGGYT 641 GK++ R + G +++ + LST+ QG + ANT G+ G+ Sbjct: 589 GKDLPFYARTTDTVGTRFKSGPWGFNLSTTH--QGRQFSDEANTIAESADGSIGEIPGFR 646 Query: 642 LVDASASYQVSPTLALEL--QVKNLANRYYEYVWINDQTRHAPGDGRAVYLSAKLTY 696 L +A ++V T ++ + NL +R Y ++ G R VY+ + ++ Sbjct: 647 LWNAQVDWKVPGTKGFDVFAGINNLTDRRYFTRTTDNNLGKLVGAPRTVYVQGRYSF 703 >gi|300310762|ref|YP_003774854.1| outer membrane ferric siderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 717 Score = 61.2 bits (147), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 81/304 (26%), Positives = 123/304 (40%), Gaps = 37/304 (12%) Query: 410 FGAYLQAVIKPVESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIR--QPKLSVVYAP 467 G Y+Q I +K++ G R D+ F SR R G++ P++ VV+ Sbjct: 434 LGLYVQDKITLAAHWKMLAGLRWDR----FEVDSRNRRNGVRSERTAHALSPRIGVVWEA 489 Query: 468 IKQASLYANWGRTFQVGAG-----AAAYKSNNNDLRPSINDGWESGVKFTPVEWLDGR-- 520 + Y +W + F G + N NDL+P + E+G+K EWLD R Sbjct: 490 LPGQHAYLSWSKNFAPVGGDVIGITPDARGNFNDLKPQFSRQVEAGIK---SEWLDRRLS 546 Query: 521 --LAWWAQRASDEVKRRLNDPT--GDSENVGRTKRSGVDLQLNLRAGKRTTMWLA---YS 573 LAW+ + R + DP G E G + G++L + G+ W ++ Sbjct: 547 TTLAWFQL---ELFNRNVADPVRPGFFEQSGLERNRGIELSVE---GELAAPWFVRAGWA 600 Query: 574 RQRSEIITPDPTAPATIGKEIDHVPRYLFSGGVDYQATDYLKLSTSLNGQGSYYLTTANT 633 Q ++I+ +P +GK V S + Y T L +G+ Y N Sbjct: 601 IQSAKIVQAEPK---FVGKRASGVSGRGGSLFLSYAPTLGWFAELGLTVEGARYADRDNL 657 Query: 634 GGKFGGYTLVDASASYQVSPTLALELQVKNLAN-RYYEYVWINDQTRHAPGDGRAVYLSA 692 + YT D Y++ L V NLAN RYYE Q R PG RA+ L+A Sbjct: 658 -LELPAYTRWDGKMGYRLDEA-EFTLAVTNLANRRYYESATGLSQVR--PGAPRALLLTA 713 Query: 693 KLTY 696 + Sbjct: 714 AYKF 717 >gi|300309529|ref|YP_003773621.1| TonB dependent ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 704 Score = 60.8 bits (146), Expect = 3e-10, Method: Compositional matrix adjust. Identities = 75/301 (24%), Positives = 122/301 (40%), Gaps = 34/301 (11%) Query: 386 SPRFNTVNKVRVTQTRDHHFTFDTFGAYLQAVIKPVESFKIIPGYRVDKV--GGSFSDPS 443 +P V+ R+ + T+DTF YLQ + E +K + G R D+ + + Sbjct: 393 NPVLPVVDPNRLNPPTLTYGTYDTFATYLQDSLSLSEQWKALVGLRYDRYRQQSRILNLA 452 Query: 444 RGVRYGIQDYGVIRQPKLSVVYAPIKQASLYANWGRTFQVGAGAAAYKSNNNDLRPSIND 503 G +Q P+ +V+ P S Y +W R+FQ + A +NN DL P Sbjct: 453 NGSTADLQRTDTAVSPRAGLVWQPSLTQSYYLSWSRSFQPSGESFALATNNADLAPEQTR 512 Query: 504 GWESGVKFTPVEWLDGRLAWWAQRAS----------DEVKRRLNDPTGDSENVGRTKRSG 553 E+G K+ +W RAS D +K L P G VG+ + G Sbjct: 513 NTEAGAKYD----------FWEGRASATVSVFRLERDNIKSAL--PNGVVIPVGKQRTDG 560 Query: 554 VDLQL--NLRAGKRTTMWLAYSRQRSEIITPDPTAPATI---GKEIDHVPRYLFSGGVDY 608 +L +L+ G + A+ + +IT + + GK PR+ + + Sbjct: 561 AELSFSADLKQGWKLLAGYAW---LNAVITESSSFDNGVPFKGKNATLTPRHSGNLWLTR 617 Query: 609 QATDYLKLSTSLNGQGSYYLTTANTGGKFGGYTLVDASASYQVSPTLALELQVKNLANRY 668 + + L + N G+ + NT GY VDA A+Y+ S ++L + NL N Sbjct: 618 ELGEGLVVGGGANAVGARFANPGNT-VTLPGYVTVDAMAAYRTS-KYEVQLNIGNLFNTG 675 Query: 669 Y 669 Y Sbjct: 676 Y 676 >gi|300312551|ref|YP_003776643.1| ferrisiderophore receptor protein [Herbaspirillum seropedicae SmR1] Length = 755 Score = 58.5 bits (140), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 75/303 (24%), Positives = 118/303 (38%), Gaps = 20/303 (6%) Query: 408 DTFGAYLQAVIKPVESFKIIPGYRVDKVGGSFSDPSRGVRYGIQDYGVIRQPKLSVVYAP 467 DT Y +K E + + G R D SF D + G + K++VV+ P Sbjct: 459 DTVSLYALDTLKFNERWSLSLGLREDFYRTSFDDTKPNAGGPLHKSGGLFNAKVAVVFKP 518 Query: 468 IKQASLYANWGRTFQVGAGA----AAYKSN--NNDLRPSINDGWESGVKFTPVEWLDGRL 521 +SLYA + Q GA +A +SN + D P D E G K+ E LD +L Sbjct: 519 TSYSSLYALYASAQQPPGGANFNLSASRSNASHTDYAPQKTDTGEIGTKW---ELLDRKL 575 Query: 522 ----AWWAQRASDEVKRRLNDPTGDSENVGRTKRSGVDLQLNLRAGKRTTMWLAYSRQRS 577 A + ++EV+ L+ T G+ G++L + ++ YS +S Sbjct: 576 IATAALYRTEVNNEVE--LDSLTNTYFQTGKKITQGLELGVTGDLSPTVSLTAGYSHMQS 633 Query: 578 EIITPDPTAPATIGKEIDHVPRYLFSGGVDYQATDYLKLSTSLNGQGSYY----LTTANT 633 + ++ AT + + PR F+ V ++ L L G T Sbjct: 634 Q-VSRGALVSATGENFLAYAPRRSFTSWVSWRLPQGFTLGGGLRYSGGLLRGRDTNAIGT 692 Query: 634 GGKFGGYTLVDASASYQVSPTLALELQVKNLANRYYEYVWINDQTRHAPGDGRAVYLSAK 693 G Y + D ASY + ++L V NL N Y R+ PGD R + L+ Sbjct: 693 PKDTGAYWVADVMASYVIHQHAEIQLNVNNLFNNSYVRSINKSGYRYLPGDARNISLTGN 752 Query: 694 LTY 696 + Sbjct: 753 FHF 755 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,303,551 Number of extensions: 103198 Number of successful extensions: 327 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 331 Number of HSP's successfully gapped: 9 Length of query: 696 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 604 Effective length of database: 1,184,250 Effective search space: 715287000 Effective search space used: 715287000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)