BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0171.p (326 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane ... 633 0.0 gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane ... 181 1e-46 gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane ... 155 5e-39 gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane ... 122 4e-29 gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [H... 114 8e-27 >gi|300313764|ref|YP_003777856.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 633 bits (1633), Expect = 0.0, Method: Compositional matrix adjust. Identities = 322/326 (98%), Positives = 324/326 (99%) Query: 1 VRSPARAVDGIDEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERTWSRAALLQ 60 +RSPARAVDGIDEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHER WSRAALLQ Sbjct: 1 MRSPARAVDGIDEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERAWSRAALLQ 60 Query: 61 ETFAALPPGGATALKQVGPAGRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIETAIG 120 ETFAALPPGGATALKQVGPAGRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARI TAIG Sbjct: 61 ETFAALPPGGATALKQVGPAGRRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIATAIG 120 Query: 121 ERREVQLDDGSRLWLNTDTRITLDFSPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQ 180 ERREVQLDDGSRLWLNTDTRITLDF+PAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQ Sbjct: 121 ERREVQLDDGSRLWLNTDTRITLDFTPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQ 180 Query: 181 GSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPC 240 GSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPC Sbjct: 181 GSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPC 240 Query: 241 DDTQAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRL 300 DDTQAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRL Sbjct: 241 DDTQAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRL 300 Query: 301 LAQTLPVRVQQLTRYWVRVLPATPAG 326 LAQTLPVRVQQLTRYWVRVLPATPAG Sbjct: 301 LAQTLPVRVQQLTRYWVRVLPATPAG 326 >gi|300309380|ref|YP_003773472.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 336 Score = 181 bits (458), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 115/306 (37%), Positives = 167/306 (54%), Gaps = 6/306 (1%) Query: 15 ILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERTWSRAALLQETFAAL--PPGGAT 72 + A WL+E+ PL + WR P+HER W R ++ AL P A Sbjct: 20 VAERALEWLLELQTQPLAPSTLEAWEAWRSAHPDHERAWRRIESVRGRLQALASPVNAAL 79 Query: 73 ALKQVGPAG-RRKAIKLLAYALTLGPAGWLAYHALSGPEQGARIETAIGERREVQLDDGS 131 A + P+G RR+ IK LA + G W + + A + T IG++R + L+DGS Sbjct: 80 AQAALTPSGNRRQVIKTLALLVFGGGVAWGLTRSEPWQQWRADLRTDIGQQRRLVLEDGS 139 Query: 132 RLWLNTDTRITLDFSPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQGSLRPLGTRFT 191 +L LNT + + L FS +R LRL GE++V T + RPFLV T QG LGTR+ Sbjct: 140 QLVLNTHSAVNLAFSAEERRLRLISGELLVTTG--KAGGGRPFLVETAQGLAYALGTRYA 197 Query: 192 VRERDQAVEVAVLQGAVEITPASSAQQT-VLPSGMQTRFTRSGSAAPRPCDDTQAAWSRG 250 VR+R EV+V +GAV + P +AQQ VL +G Q F+ G D+T+ AW+ G Sbjct: 198 VRQRAGRTEVSVWEGAVRLVPRQAAQQARVLQAGQQAAFSAQGVGDVTTADETRMAWTDG 257 Query: 251 LLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLLAQTLPVRVQ 310 +++A M L F+ ELGRY + L PA++G+ +SG +P+AD+D V+ LA L ++ Q Sbjct: 258 VIVARGMRLDAFLAELGRYTTDRLSCAPAVSGVRISGSYPVADSDAVIATLAAALGLQAQ 317 Query: 311 QLTRYW 316 R+W Sbjct: 318 LRQRFW 323 >gi|300311747|ref|YP_003775839.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 155 bits (391), Expect = 5e-39, Method: Compositional matrix adjust. Identities = 117/335 (34%), Positives = 169/335 (50%), Gaps = 26/335 (7%) Query: 11 IDEAILTEAATWLMEMHEGPLDAA----RRSKLAQWRQRSPEHERTWSRAALLQETFAAL 66 + + +L +A WL+++ P DAA ++ +A WR P HE+ WS +E F L Sbjct: 8 LQDPVLDQAIAWLVKLRAFPGDAAVAPELQAGIAAWRAADPRHEQVWSELMASEEQFQRL 67 Query: 67 PPGGATALKQVGPAGRRKAIKLLAYALT--LGPA----GWLAYHALSGPE-------QGA 113 A + GP R + + + +G A G +A A++ + GA Sbjct: 68 VALPVPASQWHGPLKRLNQRRQHSQSRRRWMGNAMLGLGAIALGAVAARQAGLFEALDGA 127 Query: 114 RIETAIGERREVQLDDGSRLWLNTDTRITLDFSPAQRLLRLQRGEIMVLTAPDRQQPP-R 172 T G++R+++L DG+ L LNT + + L F +RLL L++GEI + T D Q P R Sbjct: 128 SYATRTGDQRQLRLGDGTALALNTSSSVQLRFDDTRRLLLLRQGEIFIDTGADAQWPHHR 187 Query: 173 PFLVATRQGSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGM-----QT 227 PF V T L+ LGTRF VR+ ++ VL+G V + A P + Sbjct: 188 PFWVQTAHARLQALGTRFDVRQHGDGTQLTVLEGRVAVHVAGVDPLVAQPGEIVDIHPDG 247 Query: 228 RFTRSGSAAPRPCDDTQAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSG 287 R R + A D T AW L+ +M LA + +L RYRSG LQ DPA+A +VSG Sbjct: 248 RLVRRAARAS-AMDPT--AWLEQSLVVRQMRLADVVAQLARYRSGWLQCDPAVADWQVSG 304 Query: 288 VFPLADTDRVLRLLAQTLPVRVQQLTRYWVRVLPA 322 VF L DTD L LAQ LP+ +++ TR+WVRV A Sbjct: 305 VFQLQDTDVALEALAQALPLTIERRTRFWVRVAAA 339 >gi|300312829|ref|YP_003776921.1| Fe2+-dicitrate sensor membrane protein [Herbaspirillum seropedicae SmR1] Length = 316 Score = 122 bits (306), Expect = 4e-29, Method: Compositional matrix adjust. Identities = 95/317 (29%), Positives = 143/317 (45%), Gaps = 13/317 (4%) Query: 10 GIDEAILTEAATWLMEMHEGPLDAARRSKLAQWRQRSPEHERTWSRAALLQETF------ 63 G DE I+ +A W++++ +G L R+ QW+ P H ++ + + F Sbjct: 2 GKDEKIVEQAIEWMLQLEQGDLPPGERAAFEQWKAADPRHAMAYANLSQSVQQFDVPRQV 61 Query: 64 AALPPGGATALKQVGPAGRRKAIKLLAYALTLGPAGWLAYHALS-GPEQGARIETAIGER 122 A P L+Q + RRKA+K +A L ++ A + T G+R Sbjct: 62 GAAPVILRNTLQQ--KSKRRKALKRIAALAGLAVGAAALLDRVTPVAALTADLHTGTGQR 119 Query: 123 REVQLDDGSRLWLNTDTRITLDFSPAQRLLRLQRGEIMVLTAPDRQQPPRPFLVATRQGS 182 + L DGS + LN + I SP R +RL GEI V A PF + T GS Sbjct: 120 QRTTLADGSIITLNARSAIDHLISPVLRQVRLLEGEIQVQIAAAN---ALPFSIETVHGS 176 Query: 183 LRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQTVLPSGMQTRFTRSGSAAPRPCDD 242 + + + ++ V Q A + + Q L +G +T F + APR Sbjct: 177 VSATAGTLMLGLQPLRTDL-VAQQAAALLSTRNGQHATLAAGQRTWFDQDEIGAPRAAGG 235 Query: 243 TQAAWSRGLLIAERMPLAQFIGELGRYRSGLLQADPAIAGLEVSGVFPLADTDRVLRLLA 302 + +W G A L + + L YR GLL+ DPA+A L +SG FPL DTDR L LA Sbjct: 236 AENSWVEGYYTASDNSLEEVVAALRPYRKGLLRLDPAVAQLRISGAFPLDDTDRALSALA 295 Query: 303 QTLPVRVQQLTRYWVRV 319 LP+ V +++ YWV + Sbjct: 296 SALPITVNRVSPYWVSI 312 >gi|300313795|ref|YP_003777887.1| transmembrane sensor protein [Herbaspirillum seropedicae SmR1] Length = 342 Score = 114 bits (286), Expect = 8e-27, Method: Compositional matrix adjust. Identities = 85/233 (36%), Positives = 120/233 (51%), Gaps = 13/233 (5%) Query: 100 WLAYHALSGPEQGARIETAIGERREVQLDDGSRLWLNTDTRITLDFSPAQRLLRLQRGEI 159 WL A A + TA G L DGSRL L+ + F +R + L +GE+ Sbjct: 114 WLGLQAYPARYLLADMRTATGHWDARTLADGSRLLLSGSSAANFHFDAQRRTVELVQGEL 173 Query: 160 MVLTAPDRQQPPRPFLVATRQGSLRPLGTRFTVRERDQAVEVAVLQGAVEITPASSAQQT 219 +V A D P RPF++ T GS+R LGTRF VR V++L+ V I P + Q Sbjct: 174 LVEVARD---PARPFVIQTADGSIRALGTRFLVRRDRAGTLVSMLESKVSIQPGADTTQA 230 Query: 220 --VLPSGMQTRFTRSGSAAPRPCDDTQA---AWSRGLLIAERMPLAQFIGELGRYRSGLL 274 VL +G + R G+A P D A AW R L++ PL + + EL R+R G + Sbjct: 231 PLVLEAGQRVRL-HGGAAGPLEHIDAAALERAWRRHQLVSNARPLTEVLDELNRHRPGRI 289 Query: 275 QADPA-IAGLEVSGVFPLADTDRVLRLLAQTLP-VRVQQLTRY--WVRVLPAT 323 D A +AGL ++ V PL DT R L+LL +P +RV+Q T Y ++ + PA+ Sbjct: 290 LYDQAQLAGLHMTVVLPLDDTQRALQLLVDGVPGLRVRQFTPYLTYIDLAPAS 342 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,141,287 Number of extensions: 50641 Number of successful extensions: 192 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 178 Number of HSP's successfully gapped: 6 Length of query: 326 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 241 Effective length of database: 1,217,395 Effective search space: 293392195 Effective search space used: 293392195 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)