BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0169.p (482 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein ... 972 0.0 gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein ... 440 e-124 gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-de... 338 4e-94 gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspir... 300 1e-82 gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herb... 253 2e-68 >gi|300313762|ref|YP_003777854.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 973 bits (2514), Expect = 0.0, Method: Compositional matrix adjust. Identities = 480/482 (99%), Positives = 480/482 (99%) Query: 1 MMKPLILIPSLLLTAALAGAAAFPLALAEPSLSVPIPPQPPVGARLLNREIKPLRPHATP 60 MMKPLILIPSLLLTAALAGAAAFPLALAEPSLSVPIPPQPPVGARLLNREIKPLRPHATP Sbjct: 1 MMKPLILIPSLLLTAALAGAAAFPLALAEPSLSVPIPPQPPVGARLLNREIKPLRPHATP 60 Query: 61 AADTALAQRIAAQGSASAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI 120 AADTALAQRIAAQGSASAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI Sbjct: 61 AADTALAQRIAAQGSASAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI 120 Query: 121 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF 180 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF Sbjct: 121 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF 180 Query: 181 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAPGSDQELARGAYLVEGL 240 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPA GSDQELARGAYLVEGL Sbjct: 181 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAAGSDQELARGAYLVEGL 240 Query: 241 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE 300 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE Sbjct: 241 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE 300 Query: 301 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYDPATAQA 360 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYD ATAQA Sbjct: 301 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYDSATAQA 360 Query: 361 AYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQ 420 AYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQ Sbjct: 361 AYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQ 420 Query: 421 PSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRKTAVPVAER 480 PSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRKTAVPVAER Sbjct: 421 PSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRKTAVPVAER 480 Query: 481 AP 482 AP Sbjct: 481 AP 482 >gi|300312638|ref|YP_003776730.1| cytochrome c precursor protein [Herbaspirillum seropedicae SmR1] Length = 441 Score = 440 bits (1131), Expect = e-124, Method: Compositional matrix adjust. Identities = 211/397 (53%), Positives = 278/397 (70%), Gaps = 5/397 (1%) Query: 77 SAEDQQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAIYSTNITPDKQHGIGDW 136 SA+ Q + RG YLA+AGDCVACHT+KGG PFAGGLA+A+PIG +YS+NITPDK++GIG++ Sbjct: 31 SADQQLVQRGEYLAKAGDCVACHTAKGGKPFAGGLAIATPIGTVYSSNITPDKENGIGNY 90 Query: 137 SYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYFSKAVPPSAQENRANDI 196 S EDF R +R G+ K G ++YPAMPYPSY+++ D++ALYAYF V NR DI Sbjct: 91 SEEDFDRALRHGIRKDGASLYPAMPYPSYAKVKPADVKALYAYFMHGVQADPAPNRGVDI 150 Query: 197 PWPLSMRWPLALWRKVFAPTPAPYTPAPGSDQELARGAYLVEGLGHCGSCHSPRAVTMQE 256 WPLSMRWPL++WRKVFAP A P + L RG YLVEGLGHCG+CH+PR V MQE Sbjct: 151 TWPLSMRWPLSIWRKVFAPAVA--VDGPEDNSPLVRGQYLVEGLGHCGACHTPRGVGMQE 208 Query: 257 KALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVEFLRTGRNQYTASFGAM 316 KAL D + FLSGG V+DG+ +LR + G+ WS+AD+V FL+TGRN ++A+FG M Sbjct: 209 KALSNDSSQ-FLSGG-VIDGYLANNLRGDGRDGLGNWSEADIVAFLKTGRNSHSAAFGGM 266 Query: 317 NDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAP-YRYDPATAQAAYDGRPDGPGARIYL 375 DV+ +S QYM++ DL+AMA+YL SL P + P YD T QA G PGA +L Sbjct: 267 ADVVANSTQYMTEEDLSAMAKYLKSLKPVKDGTPALAYDDKTHQALRKGSDQSPGAMAFL 326 Query: 376 DRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQPSTASATAGLVMAPY 435 + CAACHRS+G GY + FP+LA +P + + S IRI+L+G P T A M + Sbjct: 327 NNCAACHRSSGKGYDETFPSLALSPTVNAENPASLIRIVLEGAEMPWTHKAPTQFAMPAF 386 Query: 436 AQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRK 472 L DQ+VAEV ++I+++WGN+ + +A++VAKVRK Sbjct: 387 GSRLSDQEVAEVVTFIRSSWGNQASSVSASDVAKVRK 423 >gi|300313847|ref|YP_003777939.1| membrane-bound D-gluconate 2-dehydrogenase cytochrome c subunit [Herbaspirillum seropedicae SmR1] Length = 423 Score = 338 bits (868), Expect = 4e-94, Method: Compositional matrix adjust. Identities = 183/415 (44%), Positives = 250/415 (60%), Gaps = 16/415 (3%) Query: 65 ALAQRIAAQGS---ASAEDQQIL-RGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAI 120 ALA + A G+ A A DQ ++ RG YLARAGDC+ACH++ G ++GGLA+ S G I Sbjct: 10 ALAWSLGALGTLTTAQAADQDLVARGQYLARAGDCMACHSAAGKPAYSGGLAIDSGHGII 69 Query: 121 YSTNITPDKQHGIGDWSYEDFARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYF 180 YSTNITPDK+HGIG++S F+ +R GV G +YPAMPYPSY++++DED+ ALY YF Sbjct: 70 YSTNITPDKEHGIGNYSEAQFSAAVRHGVRADGTQLYPAMPYPSYAKVSDEDIHALYTYF 129 Query: 181 SKAVPPSAQENRANDIPWPLSMRWPLALWRKVFAPTPAPYTPAPGSDQELARGAYLVEGL 240 + V P A A+ + +P ++RW + LW VF P+ G E+ RGAYLVEGL Sbjct: 130 MQGVQPVASTPPASSMSFPFNIRWGMKLW-NVFFANDKPFREQDGWSAEIKRGAYLVEGL 188 Query: 241 GHCGSCHSPRAVTMQEKALKEDGGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVE 300 GHCGSCH+PR V M EKA + FLSGG ++GW+VPSLR G+ WS D+V+ Sbjct: 189 GHCGSCHTPRGVAMNEKASDSSQAQ-FLSGGD-LNGWAVPSLR-----GMPHWSAQDIVD 241 Query: 301 FLRTGRNQYTASFGAMNDVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYDP----A 356 +L+TGRN+ + G M+ V+E S ++ DL AMA YL +L P + R P A Sbjct: 242 YLQTGRNKTASVAGEMSLVVEHSTSHLKREDLQAMAAYLKTLSPVASSGSGRVIPQGVDA 301 Query: 357 TAQAAYDGRPDGPGARIYLDRCAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQ 416 T + G R+YLD CAACH G G FP L G V+ + ++ + +IL Sbjct: 302 TVSKLTAAKDLTLGERLYLDNCAACHFVTGRGAPGIFPVLDGATVVNAENPSALLHVILA 361 Query: 417 GGRQPSTASATAGLVMAPYAQLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVR 471 G R PST A + LVM +A L D++ A + ++++ WGN G + EV K+R Sbjct: 362 GARTPSTEKAPSILVMPGFAHRLSDEEAAALATFVRQGWGNHAGKVSEREVGKLR 416 >gi|300310719|ref|YP_003774811.1| cytochrome c protein [Herbaspirillum seropedicae SmR1] Length = 431 Score = 300 bits (769), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 170/396 (42%), Positives = 235/396 (59%), Gaps = 16/396 (4%) Query: 81 QQILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAIYSTNITPDKQHGIGDWSYED 140 +Q+ +G YLARAG+C+ACHT++GGAP+AGG A+ASP G +Y++NITPDK GIG WS +D Sbjct: 46 RQLAQGQYLARAGNCMACHTARGGAPYAGGRAIASPFGTLYTSNITPDKTTGIGQWSADD 105 Query: 141 FARLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYFSKAVPPSAQENRANDIPWPL 200 F R + G K G +YPA PY SY+RL+ D AL+AY +++PP A+ N+A+ + +P Sbjct: 106 FWRALHHGRGKEGQFLYPAFPYTSYTRLSRADSDALFAYL-QSLPPVARANQAHTLRFPY 164 Query: 201 SMRWPLALWRKVFAPTPAPYTPAPGSDQELARGAYLVEGLGHCGSCHSPRAVTMQEKALK 260 R L LWR ++ TP Y P P D + RGAYLV+G GHC +CHS R L Sbjct: 165 DQRMLLGLWRALYF-TPQSYQPDPRQDAQWNRGAYLVQGAGHCSACHSAR------NRLG 217 Query: 261 EDGGRLFLSGGQV-VDGWSVPSLRNEHGG--GIAGWSQADLVEFLRTGRNQYTASFGAMN 317 L L+GG + W P L HGG G+ WS AD+ L+TG + + + G M+ Sbjct: 218 ASIEPLALAGGVIPALQWYAPPL---HGGADGLQDWSTADIAALLKTGVSPHAVTLGPMS 274 Query: 318 DVIEDSMQYMSDADLNAMARYLLSLPPRQQAAPYRYDPATAQAAYDGRPDGPGARIYLDR 377 +++ S+QY++DAD++AMA YL SLP AA PA+ + A R G ++Y Sbjct: 275 EIVGRSLQYLNDADVSAMAVYLKSLP--ASAADDAARPASVEPAEAERIMKQGKQLYGTL 332 Query: 378 CAACHRSNGTGYGKAFPALAGNPVLQTSDATSAIRIILQGGRQPSTASATAGLVMAPYAQ 437 C CH G G +P LAGN + +A+R +L GG PSTA M P+ Sbjct: 333 CVDCHGGKGQGSAPDYPPLAGNRAIAGPHPANAVRAVLNGGFPPSTAGNPYPFGMPPFGP 392 Query: 438 LLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRKT 473 L DQ+VA V SY++ +WGN+GG +AAEV + R Sbjct: 393 QLSDQEVAAVVSYVRNSWGNQGGLVSAAEVNRYRSV 428 >gi|300313053|ref|YP_003777145.1| gluconate 2-dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 499 Score = 253 bits (646), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 158/412 (38%), Positives = 219/412 (53%), Gaps = 32/412 (7%) Query: 83 ILRGAYLARAGDCVACHTSKGGAPFAGGLALASPIGAIYSTNITPDKQHGIGDWSYEDFA 142 I RG YLA A DC+ACHT+ G P AGGLA+ASP+G I +TNITP K HGIG+++ E FA Sbjct: 53 IERGHYLAIASDCIACHTAPRGKPMAGGLAIASPVGEIIATNITPSKTHGIGNYTEEQFA 112 Query: 143 RLMRTGVTKAGYTVYPAMPYPSYSRLTDEDMQALYAYFSKAVPPSAQENRANDIPWPLSM 202 +R GV G +YPAMPY +Y+ L+D D+QALYAYF K V P R D+P+P+++ Sbjct: 113 AALRRGVRADGANLYPAMPYTAYAALSDADVQALYAYFMKGVAPVDVATRPTDLPFPMNL 172 Query: 203 RWPLALWRKVFAPTPAPYTPAPGSDQELARGAYLVEGLGHCGSCHSPRAVTMQEKALKED 262 RW + W +F P P P + RG YL EG HC +CH+PR MQEK Sbjct: 173 RWSMKAWNALFL-KEQPLPPQPQRSAQWLRGRYLAEGPAHCSTCHTPRGWLMQEKP---- 227 Query: 263 GGRLFLSGGQVVDGWSVPSLRNEHGGGIAGWSQADLVEFLRTGRNQYTA-SFGAMNDVIE 321 L L+G Q V W P++ + GI WSQ DLV +LRTGR + A + G+M + I Sbjct: 228 --DLQLAGAQ-VGPWYAPNITSHPSAGIGSWSQDDLVAYLRTGRLEGRAQAAGSMAEAIT 284 Query: 322 DSMQYMSDADLNAMARYLLSLPP----RQQAAPYRYD---PATAQAAYDGRP---DGP-- 369 S +++ DL A+A Y+ P Q + R+D A A + GR + P Sbjct: 285 HSFSRLTEEDLRAIAVYIKDRPAIATGDAQGSGSRFDQGKAGNALAQFRGRTLSAESPEE 344 Query: 370 --GARIYLDRCAACHRSNGTGYGKA-FPALAGNPVLQTSDATSAIRIILQGGRQPSTASA 426 GARI+ CA+CH NG G +P+L N +A + I IL G + + Sbjct: 345 IRGARIFSASCASCHGYNGQGSRDGYYPSLFRNSATAGVNANNLIATILYGVDRETEQGG 404 Query: 427 TAGLVMAPYA------QLLDDQQVAEVTSYIQTAWGNRGGTTTAAEVAKVRK 472 + M P+ L + VA +++Y+ +G T A +V +R+ Sbjct: 405 H--VFMPPFGDQPNALNKLSNADVAALSNYLLKYYGKPAATVKAEDVEVIRQ 454 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,430,039 Number of extensions: 60063 Number of successful extensions: 264 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 218 Number of HSP's successfully gapped: 8 Length of query: 482 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 393 Effective length of database: 1,198,455 Effective search space: 470992815 Effective search space used: 470992815 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)