BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0168.p (593 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbas... 1235 0.0 gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspi... 643 0.0 gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like prot... 511 e-146 gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspi... 83 6e-17 gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspi... 80 5e-16 >gi|300313761|ref|YP_003777853.1| gluconate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 1235 bits (3196), Expect = 0.0, Method: Compositional matrix adjust. Identities = 590/593 (99%), Positives = 593/593 (100%) Query: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY 60 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY Sbjct: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY 60 Query: 61 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR 120 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR Sbjct: 61 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR 120 Query: 121 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN 180 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN Sbjct: 121 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN 180 Query: 181 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF 240 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF Sbjct: 181 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF 240 Query: 241 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDVN 300 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVD+N Sbjct: 241 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDIN 300 Query: 301 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFDP 360 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFF+P Sbjct: 301 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFEP 360 Query: 361 GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSPIWCNPSGAKPISGIAVPSGTPRW 420 GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSPIWCNPSG+KPISGIAVPSGTPRW Sbjct: 361 GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSPIWCNPSGSKPISGIAVPSGTPRW 420 Query: 421 GRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDIRM 480 GRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDIRM Sbjct: 421 GRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDIRM 480 Query: 481 SRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYL 540 SRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYL Sbjct: 481 SRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYL 540 Query: 541 QSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA 593 QSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA Sbjct: 541 QSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA 593 >gi|300312639|ref|YP_003776731.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 593 Score = 643 bits (1659), Expect = 0.0, Method: Compositional matrix adjust. Identities = 306/595 (51%), Positives = 396/595 (66%), Gaps = 4/595 (0%) Query: 1 MANKNMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEY 60 MA K K VDVV+VG GWTG+IM E+TEAG V+ALERG RDT D YPK ++EL+Y Sbjct: 1 MAIKKDK-VDVVMVGLGWTGSIMGMELTEAGLSVLALERGDMRDTNTDTPYPKAVDELKY 59 Query: 61 QQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELR 120 R LFQ+L++ + T R D A+PYRQ F G+GVGGAG HW+G +R LPEEL Sbjct: 60 SVRGALFQDLSRETVTIRHTLSDVAVPYRQHGSFLLGDGVGGAGFHWNGMMYRNLPEELE 119 Query: 121 MRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGN 180 MR+H+E++YGK FIP+DMT+QD GV+Y+ELEP++DF+EK+M SG A + G++V GN Sbjct: 120 MRTHHEQKYGKKFIPEDMTIQDHGVTYKELEPFYDFSEKVMAVSGKAGNLKGQIV-PGGN 178 Query: 181 PFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTF 240 P E +RSD FP P + QY A+L KA + G HP+ P+ANA+ P+ N YG ++GPC + Sbjct: 179 PLEGERSDEFPTPPLQYQYSASLVEKAMRDVGYHPYPAPAANASQPFTNPYGVRIGPCNY 238 Query: 241 CGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDVN 300 CGFC Y CY YSKASP I+P L + FE+R+ V+++ LD+S K+ATGVTY+D Sbjct: 239 CGFCENYGCYMYSKASPQTTILPVLLKRKNFEVRTKAQVIKVNLDASGKRATGVTYIDAQ 298 Query: 301 GDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFDP 360 G V QPA +V+ + F +N RL LLSGIGKPYDPV+ G VG+N A+Q IN Sbjct: 299 GREVEQPADLVLLTAFQTHNVRLLLLSGIGKPYDPVTGEGVVGKNFAYQYNGGINVIMPK 358 Query: 361 GKNINGFIGAGGNGVAVDDFNGDHMDHGPLGFVGGSPIWCNPSGAKPI--SGIAVPSGTP 418 G N FIG G GV +DD N D DHGPLGF+GG+ I G +PI +G P Sbjct: 359 GTQFNPFIGTGAGGVGMDDLNADQFDHGPLGFIGGASIRHVRYGGRPIKQAGTMPGEKVP 418 Query: 419 RWGRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDI 478 WG WK +V+D+Y T++ + G+ M YRD Y+ LDP Y+DA GQPLLR T +WK+N+ Sbjct: 419 SWGSEWKASVQDAYQRTLTIGMSGSVMAYRDCYLSLDPTYRDANGQPLLRLTLNWKENEG 478 Query: 479 RMSRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNR 538 +M Y+ Q K+ + ++ V+ G++ D R YQ+TH GG MGT P SVVN+ Sbjct: 479 KMLTYIASQMEKVGQAMNPEKVVKAVRKTGSSWDTRVYQSTHLTGGAIMGTTPKNSVVNK 538 Query: 539 YLQSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA 593 YLQSWDV NVF G+ F Q +GYNPTG AL Y SA+AIREQYL +P PLVQA Sbjct: 539 YLQSWDVPNVFVYGACAFPQNMGYNPTGLVTALAYHSAKAIREQYLKNPGPLVQA 593 >gi|300313846|ref|YP_003777938.1| choline dehydrogenase-like protein [Herbaspirillum seropedicae SmR1] Length = 590 Score = 511 bits (1317), Expect = e-146, Method: Compositional matrix adjust. Identities = 257/586 (43%), Positives = 358/586 (61%), Gaps = 5/586 (0%) Query: 9 VDVVIVGFGWTGAIMAKEMTEAGQRVVALERGVYRDTYPDGAYPKTINELEYQQRFKLFQ 68 VDVVIVG GW G++MA E+ +AG +V ALERG R D AYPK ++ Y R K+F Sbjct: 9 VDVVIVGLGWAGSLMAIELAQAGLKVRALERGPDRPP-EDFAYPKPADQYGYATRNKVFA 67 Query: 69 NLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHWRILPEELRMRSHYEER 128 ++ T R T A+P R+ F PG GVGG+GLHW+ R P +L+++++ ++ Sbjct: 68 TPRDAALTVRYNTAQQALPTRKWGAFAPGTGVGGSGLHWTAVLIRATPTDLKLKTYADQA 127 Query: 129 YGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGGKVVDESGNPFEADRSD 188 Y + + +M ++D+ ++ E+EP++DF +K+ G SGT + G++ G+PFE RS+ Sbjct: 128 YKHSNLDPEMRIKDFPFTWNEIEPHYDFFDKVCGLSGTTGNLRGQI-QPGGDPFEGPRSN 186 Query: 189 NFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYGCQMGPCTFCGFCSGYA 248 +PLPA + Y LF A++ G HPF PSAN + + N YG Q+ PC +CGFCS Y Sbjct: 187 PYPLPALHDTYNNTLFADVARKLGYHPFPNPSANVSQAWTNPYGAQIAPCNYCGFCSKYP 246 Query: 249 CYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSRKKATGVTYVDVNGDTVFQPA 308 C NYSKASP I+ +++ F+ + + NV+R++L + +K A GVTYVD N VFQPA Sbjct: 247 CLNYSKASPQTTILDVVKRLPNFDYKVNANVIRVDLHADKKTARGVTYVDENMKEVFQPA 306 Query: 309 SIVIASTFAYNNARLFLLSGIGKPYDPVSNTGAVGRNIAFQMMSTINAFFDPGKNINGFI 368 IVI S+F + N RL LLSGIGKPYDP++ TG +GRN AF FF K+ N F Sbjct: 307 KIVILSSFQFANVRLMLLSGIGKPYDPITETGVIGRNYAFLSNGGATLFFK-DKHFNSFA 365 Query: 369 GAGGNGVAVDDFNGDHMDHG-PLGFVGGSPIWCNPSGAKPISGIAVPSGTPRWGRAWKQA 427 AG G +D + + D G LGF+GG+ I + + PI G A+P GTP WGR WK+ Sbjct: 366 TAGATGEMFNDISPGNFDDGVKLGFIGGAKIHSSQASGAPI-GTALPPGTPSWGRGWKEG 424 Query: 428 VKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKDAFGQPLLRFTFDWKDNDIRMSRYVTDQ 487 + D Y ++M + M YR ++DLDPNYKD +GQPLLR TFDW+ N++++ RY+ D Sbjct: 425 MIDWYDHSMKISITTTCMSYRGHFLDLDPNYKDPWGQPLLRMTFDWRQNELKLQRYLRDI 484 Query: 488 AVKIAHELKAREIKVTVKDFGAAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYLQSWDVHN 547 +KI+ EL + + + D+ Y +TH GG MG P S +N+YLQSWDVHN Sbjct: 485 VLKISKELNPDHMSESFLALDSHWDITKYVSTHNVGGAIMGDSPRDSALNKYLQSWDVHN 544 Query: 548 VFAVGSGVFAQGIGYNPTGAAVALTYWSARAIREQYLNDPRPLVQA 593 VF G F Q NPT A+T +A AI+ QYL +P PLVQA Sbjct: 545 VFVPGGNAFPQNFQANPTATIGAITLMAAHAIKTQYLKNPGPLVQA 590 >gi|300313052|ref|YP_003777144.1| choline dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 543 Score = 83.2 bits (204), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 113/494 (22%), Positives = 192/494 (38%), Gaps = 69/494 (13%) Query: 101 GGAGLHWSGCHWRILPEELRMRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKM 160 GG+ HW+G WR+ PE++R+ S Y +DW Y+ LEPY+ E Sbjct: 103 GGSTWHWAGTCWRLTPEDMRLHSLYG------------VGRDWAFGYDVLEPYYTRVEHQ 150 Query: 161 MGTSGTAYRVGGKVVDESGNPFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPS 220 +G G + + + RS +P+PA F + Q G +P+ Sbjct: 151 LGICGPS---------DPALQWPPQRSAPYPMPALPFSPGEQRFTEVVQALGYR--NIPT 199 Query: 221 A---NAAAPYVNQYGCQMGPCTFCGFCSGYACYNYSKASPNVNIMPALR--QSNLFELRS 275 A N+ PY ++ C C+ C + AL ++ + + Sbjct: 200 AQARNSGMPYDDRPAC----------CANNNCVPVCPVGAKYDAATALARLEAKGATILT 249 Query: 276 SCNVLRIELDSSRKKATGVTYVDVNGDTVFQPASIVIASTFAYNNARLFLLSGIGK-PYD 334 V R++ D++ + V Y D N + I + + +L L+S + P+ Sbjct: 250 DAVVYRVDTDAN-DRVEAVNYFDANKLSHRITGKIFVLACNGIETPKLLLMSANSRNPHG 308 Query: 335 PVSNTGAVGRNI----AFQMMSTINAFFDPGKNINGFIGAGGNGVAVDDFNGDHMDHGPL 390 + + VGRN+ M T++ + G + + +G + F +H Sbjct: 309 LANRSDQVGRNMMDHPQLTMTLTLSEPYWSG--VGPVVNSGIMETSQGAFRSEH------ 360 Query: 391 GFVGGSPIWCNPSGAKPISGIAVPSGTPRWGRAWKQAVKDSYLNTMSFDVHGANMVYRDV 450 G + N + + ++ A+ G G+A + ++ + + D+ A+ V D Sbjct: 361 --AGAYFRYNNFARNRFVTFAALKKGL--VGQALDEEIR--RMTACTADIVLAHEVLPDA 414 Query: 451 YVDLD-PNYKDAFGQPLLRFTFDWKDNDIRMSR-YVTDQAVKIAHELKAREIKVTVKDFG 508 L + KD G P +D D R + Y KIA + A +IKV+ K F Sbjct: 415 ENRLTLSSKKDWLGLPRPAIHYDVGDYTRRSAELYSLPIGRKIAEAMGATDIKVSPK-FA 473 Query: 509 AAPDLRSYQTTHWAGGVAMGTDPSTSVVNRYLQSWDVHNVFAVGSGVFAQGIGYNPTGAA 568 Q+ H GG MG DP+ SVV+ ++ D N++ G G N T + Sbjct: 474 --------QSKHIMGGTIMGLDPANSVVDPDCRAHDHDNLYLPGGGAMPSTACGNSTISM 525 Query: 569 VALTYWSARAIREQ 582 AL +A AI Q Sbjct: 526 AALGLKAADAILRQ 539 >gi|300311000|ref|YP_003775092.1| glucose dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 534 Score = 80.1 bits (196), Expect = 5e-16, Method: Compositional matrix adjust. Identities = 144/602 (23%), Positives = 234/602 (38%), Gaps = 98/602 (16%) Query: 5 NMKPVDVVIVGFGWTGAIMAKEMTEAGQRVVALERG--VYRDTY----------PDGAYP 52 N+ DVV++G G TGA++A + + G+ V+ LE G + RD D P Sbjct: 3 NLNSADVVVIGAGVTGALLATRLAKTGKSVLLLESGPRMGRDQLVERFRSSAFKSDFMAP 62 Query: 53 KTINELEYQQRFKLFQNLNKSSFTFRRKTGDSAIPYRQIAMFKPGEGVGGAGLHWSGCHW 112 + L R+ N + ++ G A + I MF GG HW+ W Sbjct: 63 YPYSPLAPHPRYTPEPN------GYLQQKGPHAFNAQYIRMF------GGTSWHWAAQLW 110 Query: 113 RILPEELRMRSHYEERYGKNFIPKDMTLQDWGVSYEELEPYFDFAEKMMGTSGTAYRVGG 172 R +P + R S Y GK DW +SY++LE Y+ AE + G G+ Sbjct: 111 RYVPNDFRQHSLYG--VGK----------DWPISYDDLEDYYYQAEVIAGVGGSP----- 153 Query: 173 KVVDESGNPFEADRSDNFPLPAQKEQYQAALFRKAAQQAGLHPFTLPSANAAAPYVNQYG 232 ESG+P R FP+ E+ A+ ++ FT+ + N G Sbjct: 154 ----ESGSP----RKHPFPM----ERVPASWLQQRVTARLAPEFTVLDDSTGRNSRNYDG 201 Query: 233 ----CQMGPCTFCGFCSGYACYNYSKASPNVNIMPALRQSNLFELRSSCNVLRIELDSSR 288 C C C A Y+ A+ + + ++ + V R+E D + Sbjct: 202 RPACCGNNNCM--PLCPIDAQYHGGLAAQQA-------EDSGVKVITEAVVYRLEHD-EK 251 Query: 289 KKATGVTYVDVNGDTVFQPASIVIASTFAYNNARLFLLSGIGKPYDPVSNT-GAVGRNIA 347 + Y D N + A + + A +L L+S + ++N VGRN+ Sbjct: 252 GRIAAAHYYDWNKVSHRITAQTFVLAANAIETPKLLLMSISDRFPQGIANARDNVGRNLC 311 Query: 348 FQMMSTINAFFDPGKNI---NGFIGAGGNGVAVD-DFNGDHMDHGPLGFVGGSPIWCNPS 403 I FD + I G + G D DF +H +P + S Sbjct: 312 DH--PAIGVSFDVDEEIWPGRGPVSPCSIGQFRDGDFRAEH-----------APFRIDIS 358 Query: 404 GAKPISGIA---VPSGTPRWGRAWKQAVKDSYLNTMSFDVHGANMVYRDVYVDLDPNYKD 460 A ++ + V G +G+ ++ + +S + R+ V L + KD Sbjct: 359 NASQVAAVTKEVVAEGY--FGKRLEEQILFRAARRLSIKNALEQLPDRNNRVTLG-DKKD 415 Query: 461 AFGQPLLRFTFDWKDNDIRMSRYVTDQAVKIAHELKAREIKVTVKDFGAAPDLRSYQTTH 520 G P+ ++ D I+ + IA +L A IK + GA + + H Sbjct: 416 ELGLPVPALYWNVDDYVIKGTEQTRACYDAIAAKLGATNIKHSKS--GAFSNRQ-----H 468 Query: 521 WAGGVAMGTDPSTSVVNRYLQSWDVHNVFAVGSGVFAQGIGYNPTGAAVALTYWSARAIR 580 G ++MG DP+TSV + + ++ D N+F VG+GV N T A+AL +A I Sbjct: 469 ITGTLSMGLDPATSVTDAWGRAHDHENLFMVGTGVMPTVGTCNVTLTAMALALRTADKII 528 Query: 581 EQ 582 E+ Sbjct: 529 EE 530 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,934,026 Number of extensions: 84453 Number of successful extensions: 233 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 226 Number of HSP's successfully gapped: 5 Length of query: 593 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 502 Effective length of database: 1,188,985 Effective search space: 596870470 Effective search space used: 596870470 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)