BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0162.p (271 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313757|ref|YP_003777849.1| nitrate/sulfonate/bicarbonate A... 521 e-149 gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate A... 85 8e-18 gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporte... 84 2e-17 gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate A... 81 8e-17 gi|300310541|ref|YP_003774633.1| nitrate transporter permease [H... 80 2e-16 >gi|300313757|ref|YP_003777849.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 271 Score = 521 bits (1342), Expect = e-149, Method: Compositional matrix adjust. Identities = 269/271 (99%), Positives = 269/271 (99%) Query: 1 MRFLNRHPGRSGQWLLTLLPFLILALLYAVGSATRLADNPNDKLLPSLMQMADAVRHMAF 60 MRFLNRHPGRSGQWLLTLLPFLILALLYAVGSATRLADNPNDKLLPSLMQM DAVRHMAF Sbjct: 1 MRFLNRHPGRSGQWLLTLLPFLILALLYAVGSATRLADNPNDKLLPSLMQMVDAVRHMAF 60 Query: 61 MEDPRTGDYLFWADTLASLQRVLLGLGIAALAGLVLGIANGVFPVLRAGFSPLLTVMSMV 120 MEDPRTGDYLFWADTLASLQRVLLGLGIAALAGLVLGI NGVFPVLRAGFSPLLTVMSMV Sbjct: 61 MEDPRTGDYLFWADTLASLQRVLLGLGIAALAGLVLGIVNGVFPVLRAGFSPLLTVMSMV 120 Query: 121 PPLAILPILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWT 180 PPLAILPILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWT Sbjct: 121 PPLAILPILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWT 180 Query: 181 VVLRVALPQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLAMDVILPY 240 VVLRVALPQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLAMDVILPY Sbjct: 181 VVLRVALPQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLAMDVILPY 240 Query: 241 VVWITLLAWLMDWLLRAVHRRFFPWAEGGRA 271 VVWITLLAWLMDWLLRAVHRRFFPWAEGGRA Sbjct: 241 VVWITLLAWLMDWLLRAVHRRFFPWAEGGRA 271 >gi|300309985|ref|YP_003774077.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 278 Score = 84.7 bits (208), Expect = 8e-18, Method: Compositional matrix adjust. Identities = 85/263 (32%), Positives = 128/263 (48%), Gaps = 14/263 (5%) Query: 8 PGRSGQWLLTLLPFLILALLYAVGSATRLADNPNDKLLPSLMQMADAVRHMAFMEDPRTG 67 PGR W++ L A L V + RL P +LLP ++A + +A + G Sbjct: 23 PGRFTGWIVPL------AFLLLVEAGARLELIPA-RLLPPPSELAHTLWSLA-----QRG 70 Query: 68 DYLFWADTLASLQRVLLGLGIAALAGLVLGIANGVFPVLRAGFSPLLTVMSMVPPLAILP 127 + L ASL RV G I + + LG+ G+ A P + +P LA +P Sbjct: 71 ELL--PHIAASLARVAAGFVIGSALAVALGLLVGISRRAEALLEPSFQALRAIPSLAWIP 128 Query: 128 ILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWTVVLRVAL 187 +L + +G+DE KV+LI IG + +L R I +L+ T G N+ ++ + L Sbjct: 129 LLLLWLGIDETPKVVLIAIGAFFPVYLNLVAGIRDIDHKLIEVGTTSGLNTQALIGHILL 188 Query: 188 PQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLAMDVILPYVVWITLL 247 P L L LR L AW+FL++AE I+AT G+GY + R D++L ++ + LL Sbjct: 189 PASLPYLFAGLRGGLSLAWMFLVAAELIAATKGVGYLLSDGRESSRPDLVLAAILILALL 248 Query: 248 AWLMDWLLRAVHRRFFPWAEGGR 270 L D LLRAV ++ W E R Sbjct: 249 GKLSDGLLRAVEQKSLGWREARR 271 >gi|300310979|ref|YP_003775071.1| alkanesulfonates ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 279 Score = 83.6 bits (205), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 53/155 (34%), Positives = 85/155 (54%) Query: 114 LTVMSMVPPLAILPILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQT 173 L ++ +P LA++P++ + G+DE +K+ L+ +GV + + H R++ L+ A++ Sbjct: 123 LQMVRNIPALALIPLVILWFGIDETAKLFLVSVGVFFPVYLNTFHGIRSVDQGLIEMAKS 182 Query: 174 LGANSWTVVLRVALPQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLA 233 G + W + V LP L +LV LR +LG W+ LI AE ISA AG+GY R +L Sbjct: 183 YGLSGWPLYRDVILPGALPAILVGLRFALGLVWVLLIVAETISAQAGIGYMTMNAREFLQ 242 Query: 234 MDVILPYVVWITLLAWLMDWLLRAVHRRFFPWAEG 268 DV+L ++ LL L D R + R + W G Sbjct: 243 TDVVLVGILLYALLGKLADTAARWLERFWLRWHPG 277 >gi|300310817|ref|YP_003774909.1| nitrate/sulfonate/bicarbonate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 256 Score = 81.3 bits (199), Expect = 8e-17, Method: Compositional matrix adjust. Identities = 56/194 (28%), Positives = 97/194 (50%) Query: 68 DYLFWADTLASLQRVLLGLGIAALAGLVLGIANGVFPVLRAGFSPLLTVMSMVPPLAILP 127 D LAS R+L G G + G+ LGI G F +R P + +PP+A + Sbjct: 57 DGTLQQSVLASSGRILAGWGAGVVIGVPLGIFMGRFETVRQLLDPYIEFFRFIPPIAFVT 116 Query: 128 ILFIVMGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWTVVLRVAL 187 + I +G ELSK+ LI ++ + +A+ L A +LGA W ++ V L Sbjct: 117 LAVIWLGPGELSKIALIFYTTVFIVTLNTIAGVQAVNPLRLRAAASLGAGQWKILSTVIL 176 Query: 188 PQMLARLLVSLRLSLGAAWLFLISAEAISATAGLGYRIFLVRRYLAMDVILPYVVWITLL 247 P + ++ R+++G ++L ++SAE +SA GLG I+ R + + + ++ + +L Sbjct: 177 PSTVPFMVTGARIAMGNSFLTVVSAEIVSAREGLGALIWTARNFSRTEWVFVGIIALGIL 236 Query: 248 AWLMDWLLRAVHRR 261 +L D +LR R+ Sbjct: 237 GYLFDRVLRVATRK 250 >gi|300310541|ref|YP_003774633.1| nitrate transporter permease [Herbaspirillum seropedicae SmR1] Length = 307 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 49/154 (31%), Positives = 86/154 (55%), Gaps = 2/154 (1%) Query: 74 DTLASLQRVLLGLGIAALAGLVLGIANGVFPVLRAGFSPLLTVMSMVPPLAILPI-LFIV 132 + LASLQRV G G+AAL G+ LG A G L F P+++++ V PLA LPI L + Sbjct: 112 NVLASLQRVGTGFGLAALVGIPLGFAIGRVEFLARMFGPIISLLRPVSPLAWLPIGLLVF 171 Query: 133 MGLDELSKVMLIVIGVTPLLARDLEHTGRAIPAELLIKAQTLGANSWTVVLRVALPQMLA 192 + + + + + P++ R +P + + A+ L + W V+ ++ P +L Sbjct: 172 KSANPAAIWSIFICSIWPMIINTAVGVQR-VPQDYMNVARVLNLSEWKVLTKILFPSVLP 230 Query: 193 RLLVSLRLSLGAAWLFLISAEAISATAGLGYRIF 226 +L +RLS+G AWL +++AE ++ G+G+ ++ Sbjct: 231 YMLTGVRLSIGTAWLVIVAAEMLTGGVGIGFWVW 264 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.330 0.143 0.441 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 739,691 Number of extensions: 25442 Number of successful extensions: 201 Number of sequences better than 1.0e-08: 13 Number of HSP's gapped: 197 Number of HSP's successfully gapped: 13 Length of query: 271 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 187 Effective length of database: 1,222,130 Effective search space: 228538310 Effective search space used: 228538310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 130 (54.7 bits)