BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0161.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313756|ref|YP_003777848.1| nitrate/sulfonate/bicarbonate A... 519 e-148 gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [H... 156 2e-39 gi|300313547|ref|YP_003777639.1| nitrate/sulfonate/bicarbonate A... 154 8e-39 gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase ... 154 9e-39 gi|300313835|ref|YP_003777927.1| sugar ABC transporter ATPase [H... 152 2e-38 >gi|300313756|ref|YP_003777848.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 519 bits (1336), Expect = e-148, Method: Compositional matrix adjust. Identities = 256/258 (99%), Positives = 256/258 (99%) Query: 1 MSYIEIRNVWQQYDQNVVLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQL 60 MSYIEIRNVWQQYDQNVVLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQL Sbjct: 1 MSYIEIRNVWQQYDQNVVLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQL 60 Query: 61 LLQGQPFPAEPDATRGVVFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGAKRRAREE 120 LLQGQPFPAEPDATRGVVFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGG KRRAREE Sbjct: 61 LLQGQPFPAEPDATRGVVFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGTKRRAREE 120 Query: 121 AAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDAGIRKEMH 180 AAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALD GIRKEMH Sbjct: 121 AAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDPGIRKEMH 180 Query: 181 TLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDKVRVDPQYPQAYGAHITYDIALNEA 240 TLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDKVRVDPQYPQAYGAHITYDIALNEA Sbjct: 181 TLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDKVRVDPQYPQAYGAHITYDIALNEA 240 Query: 241 RAREQAGKLASLITAKES 258 RAREQAGKLASLITAKES Sbjct: 241 RAREQAGKLASLITAKES 258 >gi|300309785|ref|YP_003773877.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 372 Score = 156 bits (394), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 86/225 (38%), Positives = 131/225 (58%), Gaps = 17/225 (7%) Query: 1 MSYIEIRNVWQQYDQNVVLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQL 60 M+ + IRN+ ++YD N V+ +NL +E+G+F VG SGCGKST LR++ G E S G L Sbjct: 1 MAAVSIRNLAKRYDDNEVMRDINLEIEDGEFVVFVGPSGCGKSTLLRMIAGLEEISDGDL 60 Query: 61 LLQGQPFPAEPDATRGV--VFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGAKRR-- 116 + + P + RGV VFQ Y+++PH+++ DN+A GL ++ G +K Sbjct: 61 DIGARRMNEVPASKRGVAMVFQSYALYPHMSLYDNMAFGL---------KIAGKSKAEID 111 Query: 117 -AREEAAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDAGI 175 A + AA++LH + H L+ P +LSGG +QR+AI +A+ +P V L DEP LDA + Sbjct: 112 AAVQHAAKILH---IDHLLDRKPRALSGGQRQRVAIGRAITRQPSVFLFDEPLSNLDAAL 168 Query: 176 RKEMHTLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDKVRVD 220 R +M L D T+ VTHD E TL +++V + R++ Sbjct: 169 RVKMRLEFAKLHDDLKTTMIYVTHDQIEAMTLADKIVVLSEGRIE 213 >gi|300313547|ref|YP_003777639.1| nitrate/sulfonate/bicarbonate ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 154 bits (389), Expect = 8e-39, Method: Compositional matrix adjust. Identities = 93/244 (38%), Positives = 132/244 (54%), Gaps = 11/244 (4%) Query: 3 YIEIRNVWQQYD--QNVVLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQL 60 +++ R+V+ YD +E ++L+ G+F +VG SGCGKSTF++L G ++P++G + Sbjct: 5 FVDFRHVYLAYDGAAEFAVEDIDLQTRAGEFIAIVGPSGCGKSTFMKLATGLKQPNRGSV 64 Query: 61 LLQGQPFPAEPDATRGVVFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGAKRRAREE 120 ++ GQ P G+ FQ ++ P LDNV + LE+ R F K E Sbjct: 65 VIDGQTVTG-PLKFVGMAFQAPTLLPWRTTLDNVLLPLEIVEP--YRRDFKQKKAEYTER 121 Query: 121 AAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDAGIRKEMH 180 A LL VGL + +P LSGGMQQR +I +ALI P++LLLDEPFGALDA R+E+ Sbjct: 122 ALSLLKTVGLDGYADKFPWQLSGGMQQRASICRALIHEPKMLLLDEPFGALDAFTREELW 181 Query: 181 TLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDK------VRVDPQYPQAYGAHITYD 234 +L DLW + V +VTHDL E L + V K VR + P+ TY Sbjct: 182 CVLRDLWAERRFNVILVTHDLREAAFLADTIYVMSKRPGRIVVRRENPLPRPCPLETTYT 241 Query: 235 IALN 238 N Sbjct: 242 DGFN 245 >gi|300311645|ref|YP_003775737.1| taurine ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 154 bits (388), Expect = 9e-39, Method: Compositional matrix adjust. Identities = 85/196 (43%), Positives = 119/196 (60%), Gaps = 10/196 (5%) Query: 19 LERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQLLLQGQPFPAEPDATRGVV 78 L+R++L + G F +G SGCGKST L + G S+G++ L GQP P A RGVV Sbjct: 22 LDRVSLDIPNGGFVVALGTSGCGKSTLLNAMAGFLPLSEGRISLDGQPVSG-PGAERGVV 80 Query: 79 FQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGAKRRAREEAAQLLHRVGLGHALELYP 138 FQ+ S+ P +V DNVA+GL + G +K +RE A +LL VGL P Sbjct: 81 FQKDSLLPWKSVQDNVALGL---------KFAGVSKSDSRERAQELLRLVGLQDFARAAP 131 Query: 139 SSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDAGIRKEMHTLLLDLWKDSGLTVFMVT 198 LSGGM+QR+ +A+AL P++LL+DEPFGALD+ R++M LL+ +W +G VF +T Sbjct: 132 YELSGGMRQRVGLARALAPNPKILLMDEPFGALDSMTREQMQELLVSVWARTGKQVFFIT 191 Query: 199 HDLSEGFTLGTRLLVF 214 H + E LGT ++V Sbjct: 192 HSIEEALFLGTEVIVM 207 >gi|300313835|ref|YP_003777927.1| sugar ABC transporter ATPase [Herbaspirillum seropedicae SmR1] Length = 377 Score = 152 bits (385), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 88/222 (39%), Positives = 127/222 (57%), Gaps = 12/222 (5%) Query: 1 MSYIEIRNVWQQYDQNV-VLERLNLRVEEGQFCTLVGASGCGKSTFLRLLLGQERPSKGQ 59 M+++ I+ + + YD VL LNL + +G+FC LVG SGCGKST LR+L G E S G+ Sbjct: 1 MAHVNIKQLRKTYDGRADVLAGLNLDIRDGEFCVLVGPSGCGKSTLLRMLCGLEEISGGE 60 Query: 60 LLLQGQPFPAEPDATRGV--VFQRYSVFPHLNVLDNVAIGLELPRSALLGRLFGGAKRRA 117 L + GQ P A RG+ VFQ Y+++PH+NV N+A GL++ G R Sbjct: 61 LAIGGQVVNHLPPAERGIAMVFQSYALYPHMNVYKNMAFGLKVA-----GNSKSDIDARI 115 Query: 118 REEAAQLLHRVGLGHALELYPSSLSGGMQQRLAIAQALIVRPRVLLLDEPFGALDAGIRK 177 R AA L + H L+ P LSGG +QR+AI +A++ +PR+ L DEP LDA +R Sbjct: 116 RHAAAIL----KIDHLLQRLPRELSGGQRQRVAIGRAIVRQPRLFLFDEPLSNLDAALRV 171 Query: 178 EMHTLLLDLWKDSGLTVFMVTHDLSEGFTLGTRLLVFDKVRV 219 + + L + T+ VTHD E TLG +++V + R+ Sbjct: 172 QTRLEIAKLHRQLAATIVYVTHDQVEAMTLGDKIVVMHEGRI 213 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.139 0.405 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 811,144 Number of extensions: 34502 Number of successful extensions: 497 Number of sequences better than 1.0e-08: 130 Number of HSP's gapped: 272 Number of HSP's successfully gapped: 146 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)