BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0158.p (1207 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum... 2427 0.0 gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylas... 347 3e-96 gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin sub... 338 2e-93 gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspiril... 324 3e-89 gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2... 181 3e-46 >gi|300313753|ref|YP_003777845.1| urea amidolyase [Herbaspirillum seropedicae SmR1] Length = 1207 Score = 2427 bits (6289), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1196/1207 (99%), Positives = 1201/1207 (99%) Query: 1 MFDHLLIANRGAIACRILRTLHTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MFDHLLIANRGAIACRILRTL TLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY Sbjct: 1 MFDHLLIANRGAIACRILRTLRTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFADACEAAGIAFVGPTPAQLRIFGLKHTAR 120 LAVDKILAAARDSGAQAIHPGYGFLSENAAFA+ACEAAGIAFVGPTPAQLRIFGLKHTAR Sbjct: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFAEACEAAGIAFVGPTPAQLRIFGLKHTAR 120 Query: 121 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDAF 180 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAEL DAF Sbjct: 121 ALAREQGVPMLEGTDLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELADAF 180 Query: 181 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEE 240 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEE Sbjct: 181 DSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEE 240 Query: 241 TPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT 300 TPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT Sbjct: 241 TPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVT 300 Query: 301 EQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLTAV 360 EQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLTAV Sbjct: 301 EQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLTAV 360 Query: 361 EFPPADGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVE 420 +FPP DGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVE Sbjct: 361 DFPPVDGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVE 420 Query: 421 TNQHYLRQILADIPFASGQPWTRCLEQLVYRADTVEVLAPGTQTTVQDHPGRIGYWAVGV 480 TNQHYLRQILAD+PFASGQPWTRCLEQLVYRADTVEVLAPGTQTTVQDHPGRIGYWAVGV Sbjct: 421 TNQHYLRQILADVPFASGQPWTRCLEQLVYRADTVEVLAPGTQTTVQDHPGRIGYWAVGV 480 Query: 481 PPSGPMDERALRLGNRLLGNAEEAAGLEVTMSGPILRFNTAAMVVVTGAVIPVLLDGVAQ 540 PPSGPMDERALRLGNRLLGNAEEAAGLEVTMSGPILRFNTAAMVVVTGAVIPVLLDGVAQ Sbjct: 481 PPSGPMDERALRLGNRLLGNAEEAAGLEVTMSGPILRFNTAAMVVVTGAVIPVLLDGVAQ 540 Query: 541 PMATVLHIAAGSTLKLGAISGAGARSYLAVRGGVQAPTYLGSRSTFTLGQFGGHAGRALA 600 PMATVLHIAAGSTLKLGAISGAGARSYLAVRGGVQ PTYLGSRSTFTLGQFGGHAGRALA Sbjct: 541 PMATVLHIAAGSTLKLGAISGAGARSYLAVRGGVQVPTYLGSRSTFTLGQFGGHAGRALA 600 Query: 601 SGDILHLAPLAADAMTQAGQTLPAALYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFAT 660 SGDILHLAPLAADAMTQAGQTLPAALYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFAT Sbjct: 601 SGDILHLAPLAADAMTQAGQTLPAALYPALEAVRQVRVIYGPHGAPEYFTPAYMETFFAT 660 Query: 661 DWEVHFNSSRTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPD 720 DWEVHFNSSRTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPD Sbjct: 661 DWEVHFNSSRTGVRLIGPKPEWVRDSGGEAGLHPSNIHDNPYAVGAVDFTGDMPVILGPD 720 Query: 721 GPSLGGFVCPVTVIEADLWQIGQLKAGDKVRFVPVEVNTARRIALAAQAGVASLQAQPVD 780 GPSLGGFVCPVTVIEADLWQIGQLKAGDKVRFVPVEVNTARRIALAAQAGVASLQ QPVD Sbjct: 721 GPSLGGFVCPVTVIEADLWQIGQLKAGDKVRFVPVEVNTARRIALAAQAGVASLQPQPVD 780 Query: 781 WSPAPLASPIVLDLGQDERRLVARLSGDANLLLEVGSPQLDLVLRFRVHALITALEEQAL 840 WSPAPLASPIVLDLGQDERRLVARLSGDANLLLEVGSPQLDLVLRFRVHALITALEEQAL Sbjct: 781 WSPAPLASPIVLDLGQDERRLVARLSGDANLLLEVGSPQLDLVLRFRVHALITALEEQAL 840 Query: 841 PGVIDLTPGIRSLQVHYQPEALPLPTLLQTIAGLWDAVCASENLTVSSRIVHLPLSWDDP 900 PGVIDLTPGIRSLQVHYQPEALPLPTLLQTIAGLWDAVCASENLTVSSRIVHLPLSWDDP Sbjct: 841 PGVIDLTPGIRSLQVHYQPEALPLPTLLQTIAGLWDAVCASENLTVSSRIVHLPLSWDDP 900 Query: 901 ACQLAIEKYMTTVRRDAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGA 960 ACQLAIEKYMTTVR+DAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGA Sbjct: 901 ACQLAIEKYMTTVRKDAPWCPSNLEFIRRINDLKDIGAVRETVFDASYLVMGLGDVYLGA 960 Query: 961 PVATPLDPRHRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRY 1020 PVATPLDPRHRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRY Sbjct: 961 PVATPLDPRHRLVTTKYNPARTWTAENSVGIGGAYLCIYGMEGPGGYQFVGRTLQMWNRY 1020 Query: 1021 REVAAFRGKPWLLRFFDQIRFYPVTAEELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQ 1080 REVAAFRGKPWLLRFFDQIRFYPVTAEELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQ Sbjct: 1021 REVAAFRGKPWLLRFFDQIRFYPVTAEELQTIRRDFPLGRFEVGIEETQLNLADYQDFLQ 1080 Query: 1081 REAESIAAFRQHQQAAFDAERQRWIASGQANYESEESSAIAQEAAPLQDGQMAVDAPIAG 1140 REA+ IAAFRQHQQAAFDAERQRWIASGQANYESEESSAIAQEAAPLQDGQMAVDAPIAG Sbjct: 1081 READGIAAFRQHQQAAFDAERQRWIASGQANYESEESSAIAQEAAPLQDGQMAVDAPIAG 1140 Query: 1141 NLWQVKVSPGQRVAQGELLMILESMKMEIHIAAPAAGVVAEVRVQPGSPVRAGQCVLVME 1200 NLWQVKVSPGQRVAQGELLMILESMKMEIHIAAPAAGVVAEVRVQPGSPVRAGQCVLVME Sbjct: 1141 NLWQVKVSPGQRVAQGELLMILESMKMEIHIAAPAAGVVAEVRVQPGSPVRAGQCVLVME 1200 Query: 1201 DTEEEVA 1207 DTEEEVA Sbjct: 1201 DTEEEVA 1207 >gi|300313973|ref|YP_003778065.1| acetyl/propionyl-CoA carboxylase subunit alpha [Herbaspirillum seropedicae SmR1] Length = 671 Score = 347 bits (891), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 215/459 (46%), Positives = 284/459 (61%), Gaps = 15/459 (3%) Query: 1 MFDHLLIANRGAIACRILRTLHTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MF +LIANRG IACR+ T L + V VYS+AD + HV D ++ LG PA ++Y Sbjct: 1 MFTKILIANRGEIACRVAATARRLGIATVAVYSDADARAKHVAACDESVRLGPAPARESY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFADACEAAGIAFVGPTPAQLRIFGLKHTAR 120 L +KI+AAA +GAQAIHPGYGFLSENA FA AC AG+ F+GP + + G K A+ Sbjct: 61 LCAEKIIAAALATGAQAIHPGYGFLSENAGFAQACAQAGLVFIGPPASAIEAMGSKAAAK 120 Query: 121 ALAREQGVPMLEGTDLLESIDDAL-AAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDA 179 AL + VP++ G E L + A R+GYPV+LK++AGGGG GMRV + A Sbjct: 121 ALMEQAAVPLVPGYHGEEQESGLLHSQADRIGYPVLLKASAGGGGKGMRVVERSEDFDAA 180 Query: 180 FDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLE 239 S +R +F D V +EKY+ R RH+E+QVF DG G + L RDCSVQRR+QKVLE Sbjct: 181 LASCKREAIASFGDDRVLVEKYLTRPRHIEIQVFADGHGHCVYLFERDCSVQRRHQKVLE 240 Query: 240 ETPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGV 299 E PAP + + A+ AA+ A+AV Y AGTVEF+ + G FYF+E+NTRLQVEH V Sbjct: 241 EAPAPGMSEERRAAMGEAAVAAARAVGYVGAGTVEFIANQ-DGSFYFMEMNTRLQVEHPV 299 Query: 300 TEQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLT- 358 TE + G+DLV W + +AAG PL Q L+ GHAI+AR+YAE+P + F P G LT Sbjct: 300 TEMITGLDLVEWQLRVAAGQALPLKQ--EQLRIHGHAIEARIYAENPEKGFLPAIGTLTR 357 Query: 359 -----AVEF-----PPADGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALD 408 AVEF P D +RID V G I+P++DPMIAKLI W P R+AA + Sbjct: 358 MRSAAAVEFRLGQSAPGDPAHVRIDAGVREGDAISPFYDPMIAKLIVWGPDRDAALRNMQ 417 Query: 409 AALRATLLYGVETNQHYLRQILADIPFASGQPWTRCLEQ 447 AL + G+ TN +L++++A F++ T +E+ Sbjct: 418 RALAQYQVVGLATNIAFLQRLVAGQAFSTAALDTGLIER 456 >gi|300313399|ref|YP_003777491.1| acyl-CoA carboxylase biotin subunit [Herbaspirillum seropedicae SmR1] Length = 462 Score = 338 bits (867), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 202/453 (44%), Positives = 286/453 (63%), Gaps = 7/453 (1%) Query: 4 HLLIANRGAIACRILRTLHTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTYLAV 63 LLIANRG IA RI+R L +T V S+AD SL AD +G A ++YL V Sbjct: 7 KLLIANRGEIAVRIIRAAQALGITTVAACSDADTDSLAARMADEVQRIGPARADRSYLNV 66 Query: 64 DKILAAARDSGAQAIHPGYGFLSENAAFADACEAAGIAFVGPTPAQLRIFGLKHTARALA 123 D +L AARDSGA A+HPGYGFLSENAAFA+A AAG+ FVGP +R G K AR A Sbjct: 67 DALLKAARDSGADALHPGYGFLSENAAFAEAVNAAGLIFVGPQADTIRRMGDKAEARRTA 126 Query: 124 REQGVPMLEGT-DLLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGDAFDS 182 GVP++ G+ LE + ALA A +GYP+++K++AGGGG G+R+ R EL F Sbjct: 127 AAAGVPVVPGSAGELEDLATALACADEIGYPLLIKASAGGGGRGIRMARDATELAREFPL 186 Query: 183 VRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVLEETP 242 + Q F A V++E++I RARH+EVQ+ GDG + L R+CS+QRR QKVLEE P Sbjct: 187 AQAEAQAAFGSAAVYLERFIRRARHIEVQILGDGE-RAVHLFERECSLQRRRQKVLEEAP 245 Query: 243 APNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHGVTEQ 302 +P L +ALC +A++LA+ ++YR AGT+E+++D +G+F+F+E+NTR+QVEH VTE Sbjct: 246 SPALTPAQRQALCDSAVRLAERLHYRGAGTLEYLFDDESGEFFFIEMNTRIQVEHPVTEM 305 Query: 303 VWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLTAVEF 362 + G+DLV+ M+ +A G+ PL + ++ +G A++ R+ AEDP R F PCPG + +++ Sbjct: 306 ITGIDLVQAMLRIAGGE--PLGLQQSDIRMQGAALEMRINAEDPERNFFPCPGTVAELQW 363 Query: 363 PPADGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYGVETN 422 P G+ +R+++ + AG I PY+D ++AKL+ R A AA+ AT + G+ T Sbjct: 364 P--QGEGIRVESHLYAGYRIPPYYDSLLAKLVVHGKDRAQAIARAQAAVLATRITGMATT 421 Query: 423 QHYLRQILADIPFASGQPWTRCLEQ-LVYRADT 454 + +LAD S + T LE L R DT Sbjct: 422 LSLHQWLLADARVQSARFDTGALETWLAERTDT 454 >gi|300309729|ref|YP_003773821.1| biotin carboxylase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 324 bits (831), Expect = 3e-89, Method: Compositional matrix adjust. Identities = 182/449 (40%), Positives = 272/449 (60%), Gaps = 9/449 (2%) Query: 1 MFDHLLIANRGAIACRILRTLHTLDVTGVCVYSEADLGSLHVLQADRAISLGEGPAAQTY 60 MF+ +LIANRG IA RI R L + V V+SEAD + +V AD ++ +G P+ +Y Sbjct: 1 MFEKILIANRGEIALRIQRACRELGIKTVVVHSEADREAKYVKLADESVCIGPAPSTLSY 60 Query: 61 LAVDKILAAARDSGAQAIHPGYGFLSENAAFADACEAAGIAFVGPTPAQLRIFGLKHTAR 120 L + I++AA + AQAIHPGYGFLSENA FA+ E +G F+GP +R+ G K +A+ Sbjct: 61 LNMPAIISAAEVTDAQAIHPGYGFLSENADFAERVEKSGFVFIGPRAENIRMMGDKVSAK 120 Query: 121 ALAREQGVPMLEGTD--LLESIDDALAAASRVGYPVMLKSTAGGGGIGMRVCRSDAELGD 178 GVP + G+D L ++ + + A ++GYPV++K+ GGGG GMRV ++A L + Sbjct: 121 QAMIRAGVPCVPGSDGALPDNPKEIVQIARKIGYPVIIKAAGGGGGRGMRVVHTEAALIN 180 Query: 179 AFDSVRRLGQNNFSDAGVFIEKYIERARHLEVQVFGDGRGEVIALGVRDCSVQRRNQKVL 238 A + F + V++EKY+E RH+E+Q+ D + I LG RDCS+QRR+QKV+ Sbjct: 181 AVTMTKTEAGAAFGNPEVYMEKYLENPRHVEIQILADEHKQAIWLGERDCSMQRRHQKVI 240 Query: 239 EETPAPNLPDGMAEALCAAAIKLAKAVNYRSAGTVEFVYDSLAGQFYFLEVNTRLQVEHG 298 EE PAP +P + E + + + +NYR AGT EF+Y++ +FYF+E+NTR+QVEH Sbjct: 241 EEAPAPGIPRKIIEKIGERCAEACRKMNYRGAGTFEFLYEN--EEFYFIEMNTRVQVEHP 298 Query: 299 VTEQVWGVDLVRWMIELAAGDLPPLAQLAAGLQPRGHAIQARLYAEDPGRQFQPCPGLLT 358 VTE + GVD+V+ I +AAG+ L ++ +GHAI+ R+ AEDP + F P PG +T Sbjct: 299 VTEMITGVDIVQEQIRIAAGE--KLRYRQRDIELKGHAIECRINAEDPFK-FIPSPGRIT 355 Query: 359 AVEFPPADGQALRIDTWVEAGCEIAPYFDPMIAKLISWQPTREAARLALDAALRATLLYG 418 A P G +R+D+ +G + P +D M+ K+I++ TRE A + AL ++ G Sbjct: 356 AWHVP--GGPGIRVDSHAYSGYFVPPNYDSMVGKVIAYGATREQAIRRMQIALSEMVVEG 413 Query: 419 VETNQHYLRQILADIPFASGQPWTRCLEQ 447 + TN R+++ D F G LE Sbjct: 414 ISTNIPLHRELMVDARFFEGGTNIHYLEH 442 >gi|300313401|ref|YP_003777493.1| allophanate hydrolase subunit 2 [Herbaspirillum seropedicae SmR1] Length = 325 Score = 181 bits (460), Expect = 3e-46, Method: Compositional matrix adjust. Identities = 122/310 (39%), Positives = 167/310 (53%), Gaps = 9/310 (2%) Query: 454 TVEVLAPGTQTTVQDHPGRIGYWAVGVPPSGPMDERALRLGNRLLGNAEEAAGLEVTMSG 513 T++V PG T+VQD GR GY+ +G+PPSG +D+ ALR N L+GN + LE ++ G Sbjct: 2 TIKVKQPGLATSVQDG-GREGYYHLGIPPSGALDQYALRSANLLVGNHASTSALECSLLG 60 Query: 514 PILRFNTAAMVVVTGAVIPVLLDGVAQPMATVLHIAAGSTLKLGAISGAGARSYLAVRGG 573 P L F + A+V VTGA + +DG +P+ T + +G L AGARSYLA+ GG Sbjct: 61 PQLEFRSDALVAVTGARMQPRVDGSERPLDTAFMVRSGQVLSFD-YPKAGARSYLAIAGG 119 Query: 574 VQAPTYLGSRSTFTLGQFGGHAGRALASGDILHLAPLAADAMTQAGQTLPAALYPALEAV 633 + P L SRST+ LG GG GR LA D L L + A+ G+TLP +L P + Sbjct: 120 IDVPVVLESRSTYALGALGGWQGRKLAKDDELPLGRASPKAIE--GRTLPLSLVPVYDRE 177 Query: 634 RQVRVIYGPHGAPEYFTPAYMETFFATDWEVHFNSSRTGVRLIGPKPEWV--RDSGGEAG 691 +RV+ G + T +E FF W V + R G R G + R+ AG Sbjct: 178 ATLRVVPGLY--IHRLTDEAVERFFDDTWTVGSEADRIGYRFKGGQAMQFRPREQPFGAG 235 Query: 692 LHPSNIHDNPYAVGAVDFTGDM-PVILGPDGPSLGGFVCPVTVIEADLWQIGQLKAGDKV 750 PSNI D Y +G++ G + P++L D S GG+ TVI ADL IGQL+ K Sbjct: 236 SDPSNIVDACYPIGSIQIPGGLEPIVLLRDAVSGGGYATIGTVISADLDLIGQLQPNHKA 295 Query: 751 RFVPVEVNTA 760 +FV V + A Sbjct: 296 QFVRVTLEQA 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.137 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,891,843 Number of extensions: 172846 Number of successful extensions: 643 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 622 Number of HSP's successfully gapped: 7 Length of query: 1207 Length of database: 1,619,870 Length adjustment: 96 Effective length of query: 1111 Effective length of database: 1,165,310 Effective search space: 1294659410 Effective search space used: 1294659410 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 136 (57.0 bits)