BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0157.p (471 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 961 0.0 gi|300310745|ref|YP_003774837.1| amidase family protein [Herbasp... 328 7e-91 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 110 3e-25 gi|300311162|ref|YP_003775254.1| amidase family protein [Herbasp... 97 4e-21 gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln a... 96 7e-21 >gi|300313752|ref|YP_003777844.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 471 Score = 961 bits (2484), Expect = 0.0, Method: Compositional matrix adjust. Identities = 471/471 (100%), Positives = 471/471 (100%) Query: 1 MIHSFDLRIDALTAAYRNGSLDPRTVMQRLRESAAALNPTYRLYIHLLSQAETEPYLQAL 60 MIHSFDLRIDALTAAYRNGSLDPRTVMQRLRESAAALNPTYRLYIHLLSQAETEPYLQAL Sbjct: 1 MIHSFDLRIDALTAAYRNGSLDPRTVMQRLRESAAALNPTYRLYIHLLSQAETEPYLQAL 60 Query: 61 EQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLVAQLIALGAIPVGKC 120 EQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLVAQLIALGAIPVGKC Sbjct: 61 EQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLVAQLIALGAIPVGKC 120 Query: 121 NLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVP 180 NLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVP Sbjct: 121 NLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGRVP 180 Query: 181 AALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEY 240 AALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEY Sbjct: 181 AALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEY 240 Query: 241 SRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQMEAIGGVAVPIDFS 300 SRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQMEAIGGVAVPIDFS Sbjct: 241 SRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQMEAIGGVAVPIDFS 300 Query: 301 PFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQ 360 PFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQ Sbjct: 301 PFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQ 360 Query: 361 QLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPM 420 QLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPM Sbjct: 361 QLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAVATPM 420 Query: 421 NFMKNGLPWGITVFGRAFSDQALLGVADALQRAHGRPLVGQRQIEDRRRAA 471 NFMKNGLPWGITVFGRAFSDQALLGVADALQRAHGRPLVGQRQIEDRRRAA Sbjct: 421 NFMKNGLPWGITVFGRAFSDQALLGVADALQRAHGRPLVGQRQIEDRRRAA 471 >gi|300310745|ref|YP_003774837.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 611 Score = 328 bits (840), Expect = 7e-91, Method: Compositional matrix adjust. Identities = 203/470 (43%), Positives = 281/470 (59%), Gaps = 48/470 (10%) Query: 9 IDALTAAYRNGSLDP----------------------RTVMQRLRESAAALNPTYRLYIH 46 I + AAYRNGS P R ++L++ AAAL+ Sbjct: 16 IASTLAAYRNGSSTPGKTVASLLALIEQADREEVWISRVPAKQLKQQAAALD-------- 67 Query: 47 LLSQAETEPYLQALEQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESATLV 106 L+ Q + E L+ S+PL+G+PF++KDNID+AG+PTTAACP FS V SAT+V Sbjct: 68 LMWQIKGETVLE--------SMPLFGIPFAVKDNIDVAGMPTTAACPEFSRVAETSATVV 119 Query: 107 AQLIALGAIPVGKCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSS 166 +L A GA+ VGK NLDQFATGL G RSPYGA RN++ PDY SGGSSSGS++AVALG Sbjct: 120 QRLQAAGALLVGKTNLDQFATGLVGVRSPYGAVRNAIAPDYVSGGSSSGSAVAVALGQVM 179 Query: 167 FALGTDTAGSGRVPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLA 226 FALGTDTAGSGR+PAA N+LVGLK ++GL+S G+VPACRTLDC++ F A+A H+L Sbjct: 180 FALGTDTAGSGRIPAAFNHLVGLKPSRGLISAQGLVPACRTLDCISIFAHDVADAWHVL- 238 Query: 227 LCTQSGQPDERDEYSRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVEALAQ 286 S + + LP +G R +P EF G + + LA+ Sbjct: 239 ---HSAAGYDAADAYARKLPMLGLKR-----RGYRIAIPAATEFYGDLAAQDAWDRTLAR 290 Query: 287 MEAIGGVAVP-IDFSPFLAAARLLYEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAP 345 + + GV + I F+PF AARLLY+GPWVAER + + ++P + PV+R++ +A Sbjct: 291 IAELPGVTLARIPFAPFADAARLLYQGPWVAERRAALGDFFVKQPQAVHPVVREITAQAA 350 Query: 346 QYSAVDTFRAQYRLQQLKAECDRILATVDCVVTPAYPRPVTLEELREQPVQRNSDLGWYT 405 Y AV F+ QYRL +L+ + +++L D ++ P T+ E++ PV+RNS LG+YT Sbjct: 351 DYDAVAAFQGQYRLAELRRQAEQLLEGADLMLVPTTTGMPTIAEVQADPVRRNSQLGYYT 410 Query: 406 NFMNMLDYAAVATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQRAHG 455 NF+N++D +A+A P + +GLP G+T+ G A +D L LQ G Sbjct: 411 NFVNLMDMSALAIPGLWRNDGLPAGVTLIGPAGADHLLAEAGARLQHQLG 460 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 110 bits (274), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 121/415 (29%), Positives = 187/415 (45%), Gaps = 40/415 (9%) Query: 60 LEQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAF-SYVPRESATLVAQLIALGAIPVG 118 L++Q H PL G+P +IKDNID+AG+ +TA A + VP+ AT+V L GA+ G Sbjct: 57 LDEQGGHG-PLSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHAG 115 Query: 119 KCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGSGR 178 K N+ + A G+ +G CRN + +GGSS G +A L+ A+GTDT GS R Sbjct: 116 KANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSVR 175 Query: 179 VPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERD 238 +PAAL +VGL+ T S GVVP T D + A+ + L + S Sbjct: 176 IPAALCGVVGLRPTSRRYSQHGVVPISHTRDTPGPMARSVADVALLDQIMAGS------- 228 Query: 239 EYSRANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPSLFVE-ALAQMEAIGGVAVPI 297 + A PP LR GV R + G E AL Q+ G V + Sbjct: 229 --------RDSAPLLALPPSQLRLGVLRNPSWQGLEEEVEANANAALMQLREAGVELVDV 280 Query: 298 D---FSPFLAAA-------RLLYEGP---WVAERYSTIEALLQERPD-DILPVIRDVLRK 343 D +P AA L + P ++ I+ +L E D+ +I+ L + Sbjct: 281 DLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQATLDQ 340 Query: 344 -APQYSAVDTFRAQYRLQQLKAECDRILATVDCVVTPA---YPRPVTLEE---LREQPVQ 396 P+++ + +Q L +C R VD ++ P RP+ ++ LR + V Sbjct: 341 PVPEHAYRLALEQRRTMQALYRDCFRSQG-VDALIFPTTALTARPLGEDDTVALRGERVP 399 Query: 397 RNSDLGWYTNFMNMLDYAAVATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQ 451 + ++ +++ P+ + GLP G+ + G SD+ LL VA +L+ Sbjct: 400 TFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASLE 454 >gi|300311162|ref|YP_003775254.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 450 Score = 96.7 bits (239), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 109/395 (27%), Positives = 172/395 (43%), Gaps = 29/395 (7%) Query: 69 PLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRESA--TLVAQLIALGAIPVGKCNLDQFA 126 PL GVP S+KD D+AG T A P SA +V L+ GAI +GK N+ +FA Sbjct: 68 PLEGVPVSVKDLFDVAGETTLAGSVVLRGKPVASAHAAVVQNLLKAGAILIGKTNMTEFA 127 Query: 127 TGLNGTRSPYGACRNSVHPDYPSG----GSSSGSSLAVALGLSSFALGTDTAGSGRVPAA 182 G YG +N+ + G GSSSG++++V G++ A+G+DT GS R+P+A Sbjct: 128 YSGLGINPHYGTPQNAWERNIEGGRIPGGSSSGAAISVTDGMAFAAVGSDTGGSVRIPSA 187 Query: 183 LNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDERDEYSR 242 LN L G K T +S GV+P LD + + + C ++Y Sbjct: 188 LNGLTGFKPTAARVSMQGVLPLSAYLDSIGPLAVS-------VQCCAIMDAVLAGEDYV- 239 Query: 243 ANLPHNDASAFQPPPQGLRFGVPRQLEFLGCSESPS-LFVEALAQMEAIGGVAVPIDFSP 301 A L H P +GLR P + G ++ + AL ++ A G + V + Sbjct: 240 AVLAH--------PLRGLRLLAPTNVVLDGMDDTVGQAYRTALGKLAAAGAMIVEAEVPA 291 Query: 302 FLAAARLLYEGPWV-AERYSTIEALLQERPDDILPVIRDVLRKAPQYSAVDTFRAQYRLQ 360 F A A + +G + AE Y+ AL+ E + + + SA D + Sbjct: 292 FNALAAINAKGGFTAAEAYAWHRALIAENAAGYDQRVVSRILRGKDMSAADLLDVLHARP 351 Query: 361 QLKAECDRILATVDCVVTPAY----PRPVTLEELREQPVQRNSDLGWYTNFMNMLDYAAV 416 + A+ + LA D +V P PR L+ + N + +N LD A+ Sbjct: 352 RWIAQVEAQLAGYDALVMPTVPIVAPRIAELQASDDAYYGANGKMLRNPTLINFLDGCAL 411 Query: 417 ATPMNFMKNGLPWGITVFGRAFSDQALLGVADALQ 451 + P + P G+++ D+ LL + A++ Sbjct: 412 SLPCH-APGSAPVGLSLAACGGQDKRLLSIGLAVE 445 >gi|300309904|ref|YP_003773996.1| aspartate tRNAAsn/Glu-tRNAGln amidotransferase subunit A-like protein [Herbaspirillum seropedicae SmR1] Length = 651 Score = 95.5 bits (236), Expect = 7e-21, Method: Compositional matrix adjust. Identities = 123/475 (25%), Positives = 212/475 (44%), Gaps = 44/475 (9%) Query: 5 FDLRIDALTAAYRNGSLDPRTV----MQRLR---ESAAALNPTYRLYIHLLSQAETEPYL 57 F+ I L +A G R++ + R+R + +LN L L +A+ L Sbjct: 50 FEASITHLQSAQDAGRATSRSLVLAYLARIRAYDQQGPSLNAIVTLNPKALEEADQ---L 106 Query: 58 QALEQQSPHSLPLYGVPFSIKDNIDLAGVPTTAACPAFSYVPRES-ATLVAQLIALGAIP 116 +QS PL+G+P +KDN D +PTT A + + ++ A V +L GA+ Sbjct: 107 DRERRQSGPRGPLHGIPILVKDNYDTVDMPTTGGTLALATLQAQADAFQVKRLREAGAVI 166 Query: 117 VGKCNLDQFATGLNGTRSPYGACRNSVHPDYPSGGSSSGSSLAVALGLSSFALGTDTAGS 176 +GK + + A G+ S G RN P GGSS G+ AVA ++ +G+DT GS Sbjct: 167 LGKTTMHELAAGVTTVSSLTGFTRNPYDPRRAPGGSSGGTGAAVAASFAAAGMGSDTCGS 226 Query: 177 GRVPAALNNLVGLKATKGLLSTAGVVPACRTLDCVTFFTATAAEASHLLALCTQSGQPDE 236 R+PAA NL GL+ T+GL S +GV+P T D + + + +L S D Sbjct: 227 IRIPAAHQNLFGLRTTRGLASRSGVMPLSSTQDVAAPLARSVEDLAIMLDATVGSDPQDS 286 Query: 237 RDEYSRANLPHNDASAFQPPP-QGLRFGVPRQLEFLGC----SESPSLFVEALAQMEAIG 291 + ++P + + QG R GV R L G +E + +AL Q++ G Sbjct: 287 STVDANGHIPKSYRDGLRADSLQGARIGVLRAL--FGAAPEDAEVSAAINKALQQLKDQG 344 Query: 292 GVAVPI---DFSPFLAAARLL-YEGPWVAERYSTIEALLQERPDDILPVIRDVLRKAPQY 347 + + + L+ + ++ YE + + A LQ P + + ++L + ++ Sbjct: 345 AIVTDVTIPELDGLLSGSSIIPYEFKY------DLGAYLQSHPGAPVGSLGEILARGMEH 398 Query: 348 SAVDT---FRAQYRLQ--QLKAECDRIL---ATVDCVVTPAYPRPVTLEELREQPVQRNS 399 ++ R LQ + K E ++++ + + ++T + L+ L +QR + Sbjct: 399 ELLEAGLRLRNSVDLQNPKDKEELEKVMLKRSALKSLMTDVMQKN-HLDTLVYPTIQRKA 457 Query: 400 DL-----GWYTN--FMNMLDYAAVATPMNFMKNGLPWGITVFGRAFSDQALLGVA 447 L G N A+A P+ F ++GLP + + F++ ALLG+A Sbjct: 458 ALIGEPQGGAMNCQLSATTGLPALALPVGFTEDGLPVSLELLAPEFAEPALLGLA 512 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,456,828 Number of extensions: 60973 Number of successful extensions: 218 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 204 Number of HSP's successfully gapped: 11 Length of query: 471 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 382 Effective length of database: 1,198,455 Effective search space: 457809810 Effective search space used: 457809810 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 132 (55.5 bits)