BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0150.p (249 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogena... 487 e-139 gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogena... 251 3e-68 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 129 2e-31 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 129 2e-31 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 125 4e-30 >gi|300313745|ref|YP_003777837.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 487 bits (1253), Expect = e-139, Method: Compositional matrix adjust. Identities = 247/249 (99%), Positives = 248/249 (99%) Query: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA Sbjct: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 Query: 61 DASVLPDLDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTV 120 DASVL +LDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTV Sbjct: 61 DASVLSELDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTV 120 Query: 121 QKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVS 180 QKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVS Sbjct: 121 QKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNVVS 180 Query: 181 PGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIEL 240 PGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIEL Sbjct: 181 PGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGIEL 240 Query: 241 FVDGGMAQV 249 FVDGGMAQV Sbjct: 241 FVDGGMAQV 249 >gi|300312792|ref|YP_003776884.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 251 bits (642), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 134/251 (53%), Positives = 175/251 (69%), Gaps = 4/251 (1%) Query: 1 MNKQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRA 60 M K+ K+ +VTGG+ GIGLATA+ AA+GA+V+++GRR +L+ AV IG A G+ Sbjct: 3 MAKRFEDKVVVVTGGSEGIGLATAKLFAAEGARVYVSGRRADKLEQAVREIGNGAVGVPG 62 Query: 61 DASVLPDLDTVYAQIAKSAGRLDILFANAGGG--DMMPLGAITEEHFDRIFGTNVRGVLF 118 DA+ L DLD +YA++ GRLD+LFANAG + PLG I EE FD +F NVRG+LF Sbjct: 63 DAAKLDDLDRLYARVQADHGRLDVLFANAGTASTEAKPLGMIGEEDFDGLFNLNVRGLLF 122 Query: 119 TVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRAIRVNV 178 +VQKALPL+ G +++L S V G S+Y+ASKAAVR+FARSW DLK+R IRVNV Sbjct: 123 SVQKALPLMSQGGAIVLNGSVAGVKGFPGQSLYNASKAAVRSFARSWTADLKERGIRVNV 182 Query: 179 VSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNGI 238 V+PG T + + + +AR G+ D L VPLGRLGEP EIG+AV FLAS +S++ G+ Sbjct: 183 VAPGGTETRLMRDYL--EARPGMEDVLKQVVPLGRLGEPDEIGRAVLFLASAESSYIAGV 240 Query: 239 ELFVDGGMAQV 249 ELFVDGG V Sbjct: 241 ELFVDGGSVAV 251 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 89/251 (35%), Positives = 137/251 (54%), Gaps = 16/251 (6%) Query: 5 LNGKIALVTGGT--SGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIRADA 62 L K+A++TGG +G+G +TA+ +AAQGA++ I +AE A A +GA G+ AD Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGAQHLGLVADV 62 Query: 63 SVLPDLDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITEEHFDRIFGTNVRGVLFTVQK 122 + + A + K GR+DIL NAG L IT + +DRI ++RG L+ Q Sbjct: 63 TDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLYMSQA 122 Query: 123 ALPLL--VDGASVILTSSTTS-----VLGTANFSVYSASKAAVRNFARSWALDLKDRAIR 175 LP + S++ SS ++ +LG + YSA+KA V AR+ A + + IR Sbjct: 123 VLPAMQAQQSGSIVCISSVSAQRGGGILGGPH---YSAAKAGVLGLARAMAREFGPQGIR 179 Query: 176 VNVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFV 235 VN ++PG I T +++ + + +A +PLGRLG P ++G FLASD + + Sbjct: 180 VNCITPGLISTDINKDILSAEKKA----EIAQTIPLGRLGGPDDVGGVCVFLASDLSQYC 235 Query: 236 NGIELFVDGGM 246 G+ L V+GGM Sbjct: 236 TGVTLDVNGGM 246 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 129 bits (325), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 89/249 (35%), Positives = 129/249 (51%), Gaps = 11/249 (4%) Query: 3 KQLNGKIALVTGGTSGIGLATAQELAAQGAQVFITGRRQAELDAAVASIGAAATGIR-AD 61 ++ GK+ +VTG SGIG ATA+ + +GA+V + R A L S+ T R D Sbjct: 10 QRFEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDSLPPERTAARETD 69 Query: 62 ASVLPDLDTVYAQIAKSAGRLDILFANAGGGDMMPLGAITE---EHFDRIFGTNVRGVLF 118 S + + + G+LD+L ++AG + G +TE E + R+ TNV GV + Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAG---VFAEGNVTEVSPEDWHRVQATNVNGVFY 126 Query: 119 TVQKALPLLVDGASVILTSSTTSVLGT-ANFSVYSASKAAVRNFARSWALDLKDRAIRVN 177 ++ALP L I+ ++ S L N S Y+ASK AV N R+ ALD + +R+N Sbjct: 127 GAREALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRIN 186 Query: 178 VVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAASFVNG 237 V P T ++ D L D A ++ LGR +P EI + FLAS ASFVNG Sbjct: 187 AVCPSLTHTAMTADMADDPP---LLDKFAERIALGRGADPLEIAAVITFLASPDASFVNG 243 Query: 238 IELFVDGGM 246 + L VDGG+ Sbjct: 244 VNLPVDGGL 252 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 125 bits (313), Expect = 4e-30, Method: Compositional matrix adjust. Identities = 85/252 (33%), Positives = 128/252 (50%), Gaps = 17/252 (6%) Query: 3 KQLNGKIALVTGGTSGIGLATAQELAAQGAQVFIT------GRRQAELDAAVASIGAAAT 56 ++L GK+AL+TGG SGIG A A A +GA++ I RQ + D V ++ A Sbjct: 121 RRLAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKD--VQALDAPCL 178 Query: 57 GIRADASVLPDLDTVYAQIAKSAGRLDILFANAGGGDMMP---LGAITEEHFDRIFGTNV 113 + D + + + GRLD+L NA P + I++ + F TN+ Sbjct: 179 LLPGDVGSPEFCRSAVRAVMDAYGRLDVLVNNAA--QQYPAQSIEEISQAQLEHTFRTNI 236 Query: 114 RGVLFTVQKALPLLVDGASVILTSSTTSVLGTANFSVYSASKAAVRNFARSWALDLKDRA 173 + + V++A+P L GA +I T+S T+ G+ + Y+A+K A+ F RS + L Sbjct: 237 FSMFYMVKEAMPFLTRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAG 296 Query: 174 IRVNVVSPGPIRTPGLGELVPDDARQGLFDYLASQVPLGRLGEPQEIGKAVAFLASDAAS 233 I VN V+PGPI TP L+P VPLGR G+P E+ A FLA++ AS Sbjct: 297 IHVNAVAPGPIWTP----LIPASFSAEEVASFGKNVPLGRPGQPDEVAPAYVFLATEGAS 352 Query: 234 FVNGIELFVDGG 245 ++ G L +GG Sbjct: 353 YMTGQVLHPNGG 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.135 0.374 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 752,620 Number of extensions: 30299 Number of successful extensions: 282 Number of sequences better than 1.0e-08: 51 Number of HSP's gapped: 184 Number of HSP's successfully gapped: 51 Length of query: 249 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 166 Effective length of database: 1,226,865 Effective search space: 203659590 Effective search space used: 203659590 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)