BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0147.p (315 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 636 0.0 gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 233 1e-62 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 86 4e-18 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 636 bits (1640), Expect = 0.0, Method: Compositional matrix adjust. Identities = 313/315 (99%), Positives = 315/315 (100%) Query: 1 MQDLPTRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMH 60 MQDLP+RRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMH Sbjct: 1 MQDLPSRRDFLRRSGQLALSLGAAMHLPHALAQADAYPHSSGSQRAASRLPDDACDCHMH 60 Query: 61 IYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGR 120 IYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGR Sbjct: 61 IYDPRFAWAPGAKLVHAPATVAMYRQLQQRLGTTRNVVVTPSAYGVDNSCTLDALAQLGR 120 Query: 121 SARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARRVAPLGWHLQAN 180 SARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARR+APLGWHLQAN Sbjct: 121 SARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVTTVEMMEPLARRMAPLGWHLQAN 180 Query: 181 MPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYL 240 MPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYL Sbjct: 181 MPNEVLLAHRAMLARLPVPLVFDHFARIPLQADATHPVFDFVTGLMRERRASVKLSGAYL 240 Query: 241 YSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEM 300 YSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEM Sbjct: 241 YSKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDARLLDVMISWLGSEEM 300 Query: 301 VRTVLVANPARLYQF 315 VRTVLVANPARLYQF Sbjct: 301 VRTVLVANPARLYQF 315 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 233 bits (594), Expect = 1e-62, Method: Compositional matrix adjust. Identities = 129/288 (44%), Positives = 170/288 (59%), Gaps = 4/288 (1%) Query: 32 AQADAYPHSSGSQRAASRLPDDACDCHMHIYDPRFAWAPGAKLVHAP-ATVAMYRQLQQR 90 A A + P+S+GSQ + P ACD H HIYD RF +P + ATVA YR L + Sbjct: 21 ATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAK 80 Query: 91 LGTTRNVVVTPSAYGVDNSCTLDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRF 150 LG R+VVV PS YG DN C LDAL Q G ARG+ V++D V +L+ + + GVRG+R Sbjct: 81 LGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRV 140 Query: 151 NLAVG---SVTTVEMMEPLARRVAPLGWHLQANMPNEVLLAHRAMLARLPVPLVFDHFAR 207 N VTTVE + A R+APLGWH+Q M + H A+LA LP +V DH R Sbjct: 141 NFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGR 200 Query: 208 IPLQADATHPVFDFVTGLMRERRASVKLSGAYLYSKRGAPDYDDVAPLARALVQLAPTQL 267 IP HP V L+ + +KLS Y + +G PDY D + LA+A +Q AP + Sbjct: 201 IPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPPDYPDTSLLAKAYLQAAPQRT 260 Query: 268 VWGSDWPHPTEQHKPDDARLLDVMISWLGSEEMVRTVLVANPARLYQF 315 +WGSDWPHPTE+ KPDDA L D++ +W + + +LV+NPA L+ F Sbjct: 261 LWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGF 308 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 76/268 (28%), Positives = 114/268 (42%), Gaps = 15/268 (5%) Query: 55 CDCHMHIYDPRFAWAPGAKLVHAPATVAMYRQLQQRL---GTTRNVVVTPSAYGVDNSCT 111 D H H++ A + +AP Q RL G + V+V PS G DN Sbjct: 14 IDTHAHVFTRDLPLADVRR--YAPTYDVTVEQYIARLDTHGMSHGVLVQPSFLGSDNRYL 71 Query: 112 LDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRFNLAVGSVT--TVEMMEPLARR 169 L ALA+ + RG+A+V+ VS+ +L L GV G+RFNL G+ + R Sbjct: 72 LAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDFTGVWRSTLAR 131 Query: 170 VAPLGWHLQANMPNEVLLAHRAM--LARLPVPLVFDHFARIPLQADATHPVFDFVTGLMR 227 +A LGW Q + E + R + L + + +V DHF R+ P F ++ Sbjct: 132 IAQLGW--QVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDPGFRYLLSSAA 189 Query: 228 ERRASVKLSGAYLY--SKRGAPDYDDVAPLARALVQLAPTQLVWGSDWPHPTEQHKPDDA 285 + VKLS +Y +++G PL R +L+WGSDWPH + D Sbjct: 190 GGQVWVKLSASYRNGGAEQGQRFARQAWPLLRE--HFGAQRLLWGSDWPHTQHESLTDYG 247 Query: 286 RLLDVMISWLGSEEMVRTVLVANPARLY 313 + EE R + A+L+ Sbjct: 248 HSWQQFTQLVSDEEQRRIITGQTAAQLF 275 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.134 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,056,512 Number of extensions: 46017 Number of successful extensions: 149 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 3 Length of query: 315 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 230 Effective length of database: 1,217,395 Effective search space: 280000850 Effective search space used: 280000850 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 130 (54.7 bits)