BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0146.p (445 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 877 0.0 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 358 e-100 gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 338 7e-94 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 328 5e-91 gi|300313342|ref|YP_003777434.1| major facilitator superfamily p... 300 2e-82 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 877 bits (2266), Expect = 0.0, Method: Compositional matrix adjust. Identities = 443/445 (99%), Positives = 445/445 (100%) Query: 1 MSQASISPAASAVATAAVSEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDL 60 MSQASISPAASAVATAAVSEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDL Sbjct: 1 MSQASISPAASAVATAAVSEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDL 60 Query: 61 GLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKS 120 GLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKS Sbjct: 61 GLTPSQFGLGAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKS 120 Query: 121 FALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL 180 FALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL Sbjct: 121 FALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL 180 Query: 181 HGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVL 240 HGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVL Sbjct: 181 HGMWGFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVL 240 Query: 241 AARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAVPY 300 AARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAA+PY Sbjct: 241 AARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPY 300 Query: 301 LVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLGVSAFLHTPLLKMLAITVAVASILS 360 LVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLG+SAFLHTPLLKMLAITVAVASILS Sbjct: 301 LVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILS 360 Query: 361 FQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGA 420 FQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGA Sbjct: 361 FQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGA 420 Query: 421 LLLGAVITLALGDPSRVPPTSKETA 445 LLLGAVITLALGDPSRVPPTSKETA Sbjct: 421 LLLGAVITLALGDPSRVPPTSKETA 445 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 358 bits (918), Expect = e-100, Method: Compositional matrix adjust. Identities = 191/415 (46%), Positives = 271/415 (65%), Gaps = 6/415 (1%) Query: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 EDAT KV RL+P + LCYV SYLDR+NVGFA L M +DL + + +GLGAG+FF+GYF Sbjct: 17 EDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGYF 76 Query: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 FE+PSN+ LH+ GAR+WI+RIMI+WG+IS A A+V P+ F + RFLLG+AEAGF PG+ Sbjct: 77 VFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPGV 136 Query: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEAL 198 LY T W+P A RGK TA F+ GI ++ +IG PLSG +M + G+WG GWQ + +LEA+ Sbjct: 137 ILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEAI 196 Query: 199 PAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVF 258 P+++LGVM + + DR A WLS +EK+ LE +S E++ + L F N RV+ Sbjct: 197 PSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHEES---QKEHLSLGAMFANGRVW 253 Query: 259 ALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAVPYLVGAVAMTLWARLANRS 317 A FC ++G + WLP II+ G+ VGL+ A+PY A AM L R A+ Sbjct: 254 LGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADAR 313 Query: 318 ERRLFFVAGAIVLAALSLGVSA-FLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGR 376 R + VA + + L +S + H L M ++T+A IL+ FW++P+ FL+G Sbjct: 314 RERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSGT 373 Query: 377 AAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLAL 431 AAA G+ALI S+GNL GFV P ++G++++ATQ + +A +L++G ++TL L Sbjct: 374 AAAAGIALINSLGNLAGFVSPFLVGWLKDATQSTNAGMYVLAASLVIGGLLTLTL 428 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 338 bits (866), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 173/418 (41%), Positives = 260/418 (62%), Gaps = 1/418 (0%) Query: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 E T++KV RL+PF++LCY+ +++DR N+G AAL MN DLGL+P FG + LFFI YF Sbjct: 11 EQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYF 70 Query: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 FE+PSNLAL +FGAR W++RIMI+WGLIS TA V GP S RF+LG AEAGF PG+ Sbjct: 71 IFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILGAAEAGFFPGV 130 Query: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 LY T W P A+R + A F++ IP A+ + SP+S L+++ G+ G +GW L +LE LP Sbjct: 131 VLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLEGLP 190 Query: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 V+LG++CL L DRP +A+WLS +++QWL ++ E AA+ L F+ V+ Sbjct: 191 TVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWG 250 Query: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAVPYLVGAVAMTLWARLANRSER 319 +A C + +W PQ+++ FGL Q GLV ++PYL+ +V M W R ++R+ Sbjct: 251 MALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRHSDRTGE 310 Query: 320 RLFFVAGAIVLAALSLGVSAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAA 379 R + A ++L L +++ ++ +L +T+ + SF+ FWA+ S +L +AA Sbjct: 311 RRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMASSWLATGSAA 370 Query: 380 GGLALIVSIGNL-GGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSR 436 GLA I ++ NL GG + +V G++++AT + L+ + G ++ I L L SR Sbjct: 371 AGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLSRKSR 428 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 328 bits (841), Expect = 5e-91, Method: Compositional matrix adjust. Identities = 180/417 (43%), Positives = 272/417 (65%), Gaps = 9/417 (2%) Query: 19 SEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGY 78 S+D KKV R++PF++LCYV++YLDR+NVGFA L M++DLG + + FGLGAGLFFIGY Sbjct: 17 SKDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLFFIGY 76 Query: 79 FFFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPG 138 F FE+PSN+ +H+ GAR+WI+RIMI+WG++S A FV F + RFLLG+AEAGF PG Sbjct: 77 FLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAGFYPG 136 Query: 139 IYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALME-LHGMWGFKGWQVLLLLEA 197 I LY T WFP R K A F+ GIPVA I+G+PLSG +M+ H G +GWQ + L+EA Sbjct: 137 IILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMFLIEA 196 Query: 198 LPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARH--GNKLKDAFTNW 255 +PAVL+GV + L + AKWL+ +EK L+ ++ + ++H G +KDA Sbjct: 197 IPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFGAIVKDA---- 252 Query: 256 RVFALAGANFCGIIGSLSIGLWLPQIIREFGLPSH-QVGLVAAVPYLVGAVAMTLWARLA 314 RV+ + F ++G + LW+P +++ G+ + ++GL++A+P+ +AM L R A Sbjct: 253 RVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGRSA 312 Query: 315 NR-SERRLFFVAGAIVLAALSLGVSAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFL 373 +R ERR V A++ +G + F + + ++++A A +L+ FW++P+ FL Sbjct: 313 DRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTAFL 372 Query: 374 TGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLA 430 +G AAA G+A I S+GNL GFV P +IG++++ T + +A L++G++ TLA Sbjct: 373 SGAAAAVGIAAINSVGNLAGFVSPYLIGYLKDLTHNNATGMYMLAVMLVVGSIATLA 429 >gi|300313342|ref|YP_003777434.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 300 bits (767), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 186/448 (41%), Positives = 266/448 (59%), Gaps = 16/448 (3%) Query: 10 ASAVATAAVSEDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGL 69 A+A +AV D KKV +++P++LL YV++YLDR+NVGFA L+M DLG++ + +GL Sbjct: 2 ANASQGSAVQFDGLYKKVTWKILPWLLLGYVIAYLDRVNVGFAKLSMLSDLGMSEAAYGL 61 Query: 70 GAGLFFIGYFFFEIPSNLALHRFGARMWISRIMISWGLISMATAF---------VVGPKS 120 GAG+FF+GYF FE+PSNL LHR GA++WI+RIMI+WG+IS AF V Sbjct: 62 GAGIFFLGYFIFEVPSNLILHRVGAKIWIARIMITWGMISSLMAFTAPIAQWLGVSNETV 121 Query: 121 FALARFLLGMAEAGFTPGIYLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMEL 180 F RFLLG +EAGF PG+ LY T W+P A + A L+G P + I+G P+SGALM+ Sbjct: 122 FYTLRFLLGASEAGFFPGVILYMTYWYPAAMHSRVMAVVLLGNPFSYILGGPISGALMQF 181 Query: 181 HGMW-GFKGWQVLLLLEALPAVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNV 239 G GWQ + ++EALPA LLG++ LF+L + A AKWL+ +EK L L+ E Sbjct: 182 MGSGDSLAGWQWMFVIEALPACLLGIVILFVLSNDIASAKWLAPEEKAELARRLAAENK- 240 Query: 240 LAARHGNKLKDAFTNWRVFALAGANFCGIIGSLSIGLWLPQIIREFGLPS-HQVGLVAAV 298 + LK + ++ LA +IGS I W P IIR+ G+ S +G+++A+ Sbjct: 241 --EKVNVPLKSLYALPILWFLALVYLLMMIGSYGINFWAPTIIRDAGVKSVLDIGMISAI 298 Query: 299 PYLVGAVAMTLWARLANRSERRLFFVAGAIVLAALSLGVSA-FLHTPLLKMLAITVAVAS 357 PY++GA AM R A ++ + + ++A L+L + F H + + I +AV Sbjct: 299 PYILGATAMVFITRHAEKAGKHRSYSVAVSMVAGLALVLCTLFPHNIYITLAGIAIAVGG 358 Query: 358 ILSFQATFWAIPSGFLTGRAAAGGLALIVSIGNLGGFVGPSVIGFIREATQGFTYPLIFV 417 L+ A FW P + TG AA G+A+I S+GNLGGFVGP ++G + A Y L+ + Sbjct: 359 SLTSIALFWGFPGAYFTGATAAAGIAIINSVGNLGGFVGPYLLGALTTAFGSPRYGLMML 418 Query: 418 AGALLLGAVITLALGDPSRVPPTSKETA 445 GAL L V+ +ALG R P +A Sbjct: 419 GGALFLAGVL-IALGGRKRGPAAVTASA 445 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.435 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,338,404 Number of extensions: 57676 Number of successful extensions: 404 Number of sequences better than 1.0e-08: 20 Number of HSP's gapped: 366 Number of HSP's successfully gapped: 20 Length of query: 445 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 357 Effective length of database: 1,203,190 Effective search space: 429538830 Effective search space used: 429538830 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)