BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0143.p (308 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogen... 602 e-174 gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid... 160 1e-40 gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [H... 145 4e-36 gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogen... 144 1e-35 gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbas... 142 4e-35 >gi|300313735|ref|YP_003777827.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 308 Score = 602 bits (1553), Expect = e-174, Method: Compositional matrix adjust. Identities = 302/308 (98%), Positives = 305/308 (99%) Query: 1 VSAAKKEVVIVTGVDLAPAAVELLRDYELVYAGAKPTEDDMVKLAQQHQPVAIIVRYGGV 60 +SAAKKEVVIVTGVDLA AAVELLRDYELVYAGAKPTEDDMVKLAQ HQPVAIIVRYGGV Sbjct: 1 MSAAKKEVVIVTGVDLAQAAVELLRDYELVYAGAKPTEDDMVKLAQLHQPVAIIVRYGGV 60 Query: 61 SARVMDAAPSLRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAK 120 SARVMDAAP LRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAK Sbjct: 61 SARVMDAAPILRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAK 120 Query: 121 NVVGLDQRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAYDPYAK 180 NVVGLDQRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLA+DPYAK Sbjct: 121 NVVGLDQRMREGHWDKSTHKSLELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAHDPYAK 180 Query: 181 EAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDE 240 EAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDE Sbjct: 181 EAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDE 240 Query: 241 QALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTADAYIAMGTGAAS 300 QALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVT+DAYIAMGTGAAS Sbjct: 241 QALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTSDAYIAMGTGAAS 300 Query: 301 NVLAVLGA 308 NVLAVLGA Sbjct: 301 NVLAVLGA 308 >gi|300310832|ref|YP_003774924.1| D-isomer specific 2-hydroxyacid dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 319 Score = 160 bits (405), Expect = 1e-40, Method: Compositional matrix adjust. Identities = 95/253 (37%), Positives = 142/253 (56%), Gaps = 9/253 (3%) Query: 60 VSARVMDAAPSLRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 ++ +++AAP L+++S G+D+ D +QRG+ + G A A+ +ALI+A A Sbjct: 56 ITNAMLEAAPQLKIVSTISVGVDNFDLDYFRQRGLMLAHTPGVLTEATADTIFALILASA 115 Query: 120 KNVVGLDQRMREGHWDKSTHKS---LELQGRTLGLVGLGAIGRRVAAVAA-ALGMPVLAY 175 + VV L + ++ G W S ++ + + G+TLGL+G+G IG VA A GMP+L + Sbjct: 116 RRVVELAEYVKAGRWKGSIGEAQFGVNVHGKTLGLIGMGRIGSAVARRAHHGFGMPILYH 175 Query: 176 ----DPYAKEAPQGVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILVN 231 DP A E G + V + L AQ+D V + PLT + + M A A MK AI +N Sbjct: 176 NRRPDPEA-ERELGARYVSKEDLLAQADFVCVMLPLTPQTERMFGAPEFALMKRSAIFIN 234 Query: 232 TARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTADAY 291 +RG ++DE ALIAAL + AGLD FE EP PL ++ N V PHIG T + Sbjct: 235 ASRGRIVDEAALIAALQDKTIHGAGLDVFEVEPLPLQSPLLKLPNVVALPHIGSATHETR 294 Query: 292 IAMGTGAASNVLA 304 +AM A +N++A Sbjct: 295 LAMAELAVTNLIA 307 >gi|300310960|ref|YP_003775052.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 326 Score = 145 bits (367), Expect = 4e-36, Method: Compositional matrix adjust. Identities = 91/257 (35%), Positives = 134/257 (52%), Gaps = 11/257 (4%) Query: 60 VSARVMDAAPSLRVISKHGTGIDSIDSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACA 119 V+A + A P L+ + G ++ID AA Q G+ A+ WAL+MA A Sbjct: 57 VTAALFAANPQLKAVCNMAVGYNNIDVAAATQAGVMATNTPDVLNETTADFGWALMMAAA 116 Query: 120 KNVVGLDQRMREGHWDKSTHKSL---ELQGRTLGLVGLGAIGRRVAAVAAALGMPVLAYD 176 + + + +R G W K ++ S ++ G TLG++G+G IG+ +A + M VL Y Sbjct: 117 RRITESEHFLRAGKWKKWSYDSFVGPDIHGATLGIIGMGRIGQAIARRSLGFDMQVL-YH 175 Query: 177 PYAKEAPQ------GVQLVDLDTLFAQSDVVSLHCPLTAENKHMINAQSLARMKDGAILV 230 ++ AP+ + V + L Q+D V L P + + H I A LA MK A L Sbjct: 176 NRSRLAPELEARANHARYVSKEELLRQADHVILVLPYSKDTHHTIAAAELALMKPTATLT 235 Query: 231 NTARGGLIDEQALIAALDSGKLRAAGLDSFEKEPFTAPHPLQRVGNAVLSPHIGGVTADA 290 N ARGG++D+ ALIAAL G++ AAG+D FE EP P L + N VL+PHI + Sbjct: 236 NIARGGIVDDAALIAALREGRIAAAGVDVFENEPAFKPEFLD-LSNVVLTPHIASASTPT 294 Query: 291 YIAMGTGAASNVLAVLG 307 +AM AA N++A L Sbjct: 295 RLAMANCAADNLIAALS 311 >gi|300311988|ref|YP_003776080.1| D-3-phosphoglycerate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 310 Score = 144 bits (362), Expect = 1e-35, Method: Compositional matrix adjust. Identities = 88/266 (33%), Positives = 146/266 (54%), Gaps = 5/266 (1%) Query: 27 YELVYAGAKPTEDDMVKLAQQHQPVAIIVRYG--GVSARVMDAAPSLRVISKHGTGIDSI 84 Y+++YA + D+M+ A + VA+++ G G++A M A P L+++ G G +++ Sbjct: 23 YDILYAPDRQRRDEMI--AGPARKVAVVLTNGSTGLTAEEMRALPHLQLVCTLGAGFENV 80 Query: 85 DSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLEL 144 D A+ GI + AG N VA+H L++A +N+ LD+ R+G W ++ +L Sbjct: 81 DVAHAEAHGIEIATGAGTNEDCVADHALGLLLAILRNIPVLDRYTRDGGWRETIPLQPQL 140 Query: 145 QGRTLGLVGLGAIGRRVAAVAAALGMPVLAYDPYAKEAPQGVQLVDLDTLFAQSDVVSLH 204 G+ +G+VG+G IG+++A AAA + + ++ D+ L +D + + Sbjct: 141 AGKRVGIVGMGNIGKKIARRAAAFDAEIAYCNRKKRDDVDYHYFPDVAQLAGWADCLIVA 200 Query: 205 CPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEKEP 264 P A+ +H+INA+ L + LVN RG ++D AL AAL SG+L AGLD +E EP Sbjct: 201 APGGAQTRHLINARVLEELGPQGYLVNIGRGSIVDTDALGAALSSGRLAGAGLDVYEGEP 260 Query: 265 FTAPHPLQRVGNAVLSPHIGGVTADA 290 P L + N VL+PHI G + +A Sbjct: 261 -QPPAALIALPNVVLTPHIAGWSPEA 285 >gi|300311826|ref|YP_003775918.1| D-lactate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 330 Score = 142 bits (358), Expect = 4e-35, Method: Compositional matrix adjust. Identities = 91/241 (37%), Positives = 133/241 (55%), Gaps = 6/241 (2%) Query: 27 YELVYAGAKPTEDDMVKLAQQHQPVAIIVR--YGGVSARVMDAAPSLRVISKHGTGIDSI 84 +ELV+ + E +LA Q VA V GGV + A +R ++ TG + I Sbjct: 25 HELVFLQDR-LEAGTAQLAAGCQAVAAFVNDDLGGVVLEKL-AELGVRFVTTRSTGFNHI 82 Query: 85 DSQAAQQRGIAVKAAAGANAPAVAEHTWALIMACAKNVVGLDQRMREGHWDKSTHKSLEL 144 D AA+ G+ V A + +VAE L+ A + + R REG+++ ++L Sbjct: 83 DVAAARALGLTVARVADYSPYSVAEFAVGLLQAVNRKIARASMRTREGNFELDGLMGVDL 142 Query: 145 QGRTLGLVGLGAIGRRVAAVAAALGMPVLAYDPYAKEAPQ--GVQLVDLDTLFAQSDVVS 202 G+T+G++G G IG A + A G +L D + G + V+ LFA SDV+S Sbjct: 143 HGKTVGVIGTGKIGVLFARIMAGFGCQILGSDVHRNPDFDALGGRYVERTELFAASDVIS 202 Query: 203 LHCPLTAENKHMINAQSLARMKDGAILVNTARGGLIDEQALIAALDSGKLRAAGLDSFEK 262 LHCPLT ++++NAQSLA K G ILVNT+RGGL+D +A IAAL SG+LR +D +E+ Sbjct: 203 LHCPLTEATRYLVNAQSLALAKPGCILVNTSRGGLVDTEAAIAALKSGRLRGLAIDVYEQ 262 Query: 263 E 263 E Sbjct: 263 E 263 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.317 0.132 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 839,051 Number of extensions: 30748 Number of successful extensions: 165 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 152 Number of HSP's successfully gapped: 11 Length of query: 308 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 223 Effective length of database: 1,217,395 Effective search space: 271479085 Effective search space used: 271479085 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)