BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0142.p (431 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum serope... 862 0.0 gi|300313738|ref|YP_003777830.1| major facilitator superfamily p... 339 3e-94 gi|300311567|ref|YP_003775659.1| major facilitator superfamily p... 313 2e-86 gi|300313516|ref|YP_003777608.1| major facilitator superfamily p... 300 1e-82 gi|300311164|ref|YP_003775256.1| major facilitator superfamily p... 299 3e-82 >gi|300313734|ref|YP_003777826.1| permease [Herbaspirillum seropedicae SmR1] Length = 431 Score = 862 bits (2227), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/431 (99%), Positives = 430/431 (99%) Query: 1 METIQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF 60 METIQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF Sbjct: 1 METIQNEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGF 60 Query: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLVSACTALVQGPNSLYFVRFILG 120 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGL+SACTALVQGPNSLYFVRFILG Sbjct: 61 ASSLFFIAYFIFEVPSNLALQKFGARKWLARIMITWGLISACTALVQGPNSLYFVRFILG 120 Query: 121 AAEAGFFPGVVLYLTYWVPSTYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 AAEAGFFPGVVLYLTYWVPS YRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW Sbjct: 121 AAEAGFFPGVVLYLTYWVPSAYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGW 180 Query: 181 HWLFMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLW 240 HWLFMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLW Sbjct: 181 HWLFMLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLW 240 Query: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW 300 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW Sbjct: 241 KLFSQPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVW 300 Query: 301 WGRHSDRTGERRWHTAIPLMLIGGGLALASLAHSLAPVVLLLTMVLIGAYSFKGPFWAMA 360 WGRHSDRTGERRWHTAIPLMLIGGGLALASLA+SLAPVVLLLTMVLIGAYSFKGPFWAMA Sbjct: 301 WGRHSDRTGERRWHTAIPLMLIGGGLALASLANSLAPVVLLLTMVLIGAYSFKGPFWAMA 360 Query: 361 SSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGIL 420 SSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGIL Sbjct: 361 SSWLATGSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGIL 420 Query: 421 LFLSRKSRAGH 431 LFLSRKSRAGH Sbjct: 421 LFLSRKSRAGH 431 >gi|300313738|ref|YP_003777830.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 445 Score = 339 bits (869), Expect = 3e-94, Method: Compositional matrix adjust. Identities = 173/418 (41%), Positives = 259/418 (61%), Gaps = 1/418 (0%) Query: 11 EQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYF 70 E T++KV RL+PF++LCY+ +++DR N+G AAL MN DLGL+P FG + LFFI YF Sbjct: 20 EDATMKKVMRRLIPFILLCYVVSYLDRINVGFAALTMNKDLGLTPSQFGLGAGLFFIGYF 79 Query: 71 IFEVPSNLALQKFGARKWLARIMITWGLVSACTALVQGPNSLYFVRFILGAAEAGFFPGV 130 FE+PSNLAL +FGAR W++RIMI+WGL+S TA V GP S RF+LG AEAGF PG+ Sbjct: 80 FFEIPSNLALHRFGARMWISRIMISWGLISMATAFVVGPKSFALARFLLGMAEAGFTPGI 139 Query: 131 VLYLTYWVPSTYRARIVALFMVAIPAASFVASPVSALLLQMDGIGGLRGWHWLFMLEGLP 190 LY T W P +R + A F++ IP A+ + SP+S L+++ G+ G +GW L +LE LP Sbjct: 140 YLYFTQWFPGAWRGKATAFFLIGIPVANIIGSPLSGALMELHGMWGFKGWQVLLLLEALP 199 Query: 191 TVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWG 250 V+LG++CL L DRP +A+WLS +++QWL ++ E AA+ L F+ V+ Sbjct: 200 AVLLGVMCLFLLPDRPAKAKWLSADEKQWLENELSTEQNVLAARHGNKLKDAFTNWRVFA 259 Query: 251 MALVCACASAAGSVLNVWQPQLLKSFGLSVMQTGLVNSIPYLIASVLMVWWGRHSDRTGE 310 +A C + +W PQ+++ FGL Q GLV +IPYL+ +V M W R ++R+ Sbjct: 260 LAGANFCGIIGSLSIGLWLPQIIREFGLPSHQVGLVAAIPYLVGAVAMTLWARLANRSER 319 Query: 311 RRWHTAIPLMLIGGGLALASLAHSLAPVVLLLTMVLIGAYSFKGPFWAMASSWLATGSAA 370 R + A ++L L +++ H+ +L +T+ + SF+ FWA+ S +L +AA Sbjct: 320 RLFFVAGAIVLAALSLGISAFLHTPLLKMLAITVAVASILSFQATFWAIPSGFLTGRAAA 379 Query: 371 AGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLSRKSR 428 GLA I ++ NL GG + +V G++++AT + L+ + G ++ I L L SR Sbjct: 380 GGLALIVSIGNL-GGFVGPSVIGFIREATQGFTYPLIFVAGALLLGAVITLALGDPSR 436 >gi|300311567|ref|YP_003775659.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 435 Score = 313 bits (802), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 165/395 (41%), Positives = 246/395 (62%), Gaps = 7/395 (1%) Query: 10 LEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAY 69 E T KVTWRLLP L LCY+ +++DR N+G A LQM DL S ++G + +FF+ Y Sbjct: 16 FEDATYSKVTWRLLPLLFLCYVASYLDRVNVGFAKLQMLQDLKFSDTVYGLGAGIFFLGY 75 Query: 70 FIFEVPSNLALQKFGARKWLARIMITWGLVSACTALVQGPNSLYFVRFILGAAEAGFFPG 129 F+FEVPSN+ L K GAR W+ARIMITWG++S A V P Y +RF+LG AEAGFFPG Sbjct: 76 FVFEVPSNMILHKVGARLWIARIMITWGIISGAMAYVTTPEMFYIMRFLLGVAEAGFFPG 135 Query: 130 VVLYLTYWVPSTYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFMLEG 188 V+LYLTYW P+ R +I ALFM I + + P+S ++ M G+ G GW W+F+LE Sbjct: 136 VILYLTYWYPAARRGKITALFMTGIALSGVIGGPLSGWIMHAMPGVWGHTGWQWMFILEA 195 Query: 189 LPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHV 248 +P+++LG++ + ++ DR A WLS+E+++ L ++ E + K+H+ L +F+ V Sbjct: 196 IPSLILGVMVILYMKDRIRDADWLSEEEKRLLEANISHE---ESQKEHLSLGAMFANGRV 252 Query: 249 WGMALVCACASAAGSVLNVWQPQLLKSFGLSV-MQTGLVNSIPYLIASVLMVWWGRHSDR 307 W AL+ C ++ W P ++K+ G+S + GL+ +IPY A+ MV GR +D Sbjct: 253 WLGALIYFCFVMGLYGVSFWLPTIIKATGVSDPLNVGLLTAIPYAFAAAAMVLIGRSADA 312 Query: 308 TGERRWHTAIPLMLIGGGLALAS-LAHSLAPVVLLLTMVLIGAYSFKGPFWAMASSWLAT 366 ERRWH AIP + GL L++ H+ + LT+ +G + FW++ +++L+ Sbjct: 313 RRERRWHVAIPAFIGCIGLLLSTQYGHNTTLAMASLTLASVGILTTLPLFWSLPTAFLSG 372 Query: 367 GSAAAGLAGINAVSNLIGGGLMVNVYGWVKDATGS 401 +AAAG+A IN++ NL G + + GW+KDAT S Sbjct: 373 TAAAAGIALINSLGNL-AGFVSPFLVGWLKDATQS 406 >gi|300313516|ref|YP_003777608.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 300 bits (769), Expect = 1e-82, Method: Compositional matrix adjust. Identities = 167/416 (40%), Positives = 256/416 (61%), Gaps = 16/416 (3%) Query: 6 NEKRLEQETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLF 65 N+K + +KV WR++PFLMLCY+ A++DR N+G A LQM+ DLG S +FG + LF Sbjct: 14 NDKS-KDGLYKKVFWRIMPFLMLCYVIAYLDRVNVGFAKLQMSQDLGFSETVFGLGAGLF 72 Query: 66 FIAYFIFEVPSNLALQKFGARKWLARIMITWGLVSACTALVQGPNSLYFVRFILGAAEAG 125 FI YF+FEVPSN+ + K GAR W+ARIMITWG++SA VQ Y +RF+LG AEAG Sbjct: 73 FIGYFLFEVPSNILMHKVGARIWIARIMITWGILSAAFMFVQNATQFYILRFLLGLAEAG 132 Query: 126 FFPGVVLYLTYWVPSTYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLF 184 F+PG++LYLTYW PS RA+++A+FM IP A + +P+S ++ GGL GW W+F Sbjct: 133 FYPGIILYLTYWFPSHRRAKVIAVFMSGIPVAGILGNPLSGWIMDAFHQNGGLEGWQWMF 192 Query: 185 MLEGLPTVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFS 244 ++E +P V++G+ + +L + A+WL+DE++ L ++ +A + +K + Sbjct: 193 LIEAIPAVLIGVATVLYLDNDVKSAKWLNDEEKASLQADIDGDAKGKESKHGFG--AIVK 250 Query: 245 QPHVWGMALVCACASAAGSVLNVWQPQLLKSFGLS-VMQTGLVNSIPYLIASVLMVWWGR 303 VW M L+ L +W P L+K+ G+ ++ GL+++IP+ A + M GR Sbjct: 251 DARVWLMCLIYFSFVMGQYGLTLWMPTLVKATGVKGNLEIGLLSAIPFGCAIIAMNLIGR 310 Query: 304 HSDRTGERRWHTAIPLMLIGGGLALASL-AHSLAPVVLLLTMVLIGAYSFKGPFWAMASS 362 +DR ERRWH IP ++ G G A+L A + A + L++ G + FW++ ++ Sbjct: 311 SADRMRERRWHLVIPALMGGVGFVGAALFADNTAVSIASLSLAAAGVLTCAPLFWSLPTA 370 Query: 363 WLATGSAAAGLAGINAVSNLIGGGLMVNVY--GWVKD-----ATGSYAMALLPMVG 411 +L+ +AA G+A IN+V NL G V+ Y G++KD ATG Y +A++ +VG Sbjct: 371 FLSGAAAAVGIAAINSVGNLAG---FVSPYLIGYLKDLTHNNATGMYMLAVMLVVG 423 >gi|300311164|ref|YP_003775256.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 437 Score = 299 bits (765), Expect = 3e-82, Method: Compositional matrix adjust. Identities = 170/418 (40%), Positives = 254/418 (60%), Gaps = 6/418 (1%) Query: 12 QETVRKVTWRLLPFLMLCYLFAFIDRGNIGMAALQMNHDLGLSPKMFGFASSLFFIAYFI 71 + T RK+ WRL+PFL+ ++ A+IDR N+G A LQM DL S ++G + +FFI YFI Sbjct: 22 EVTYRKIAWRLIPFLVFLFVLAWIDRVNVGFAKLQMLQDLQFSEAVYGLGAGIFFIGYFI 81 Query: 72 FEVPSNLALQKFGARKWLARIMITWGLVSACTALVQGPNSLYFVRFILGAAEAGFFPGVV 131 FEVPSNL L+K GARK LARI I WGL S A V P S Y +RF+LG EAGFFPGVV Sbjct: 82 FEVPSNLLLEKIGARKTLARITILWGLASMAMAYVTTPTSFYILRFLLGIFEAGFFPGVV 141 Query: 132 LYLTYWVPSTYRARIVALFMVAIPAASFVASPVSALLLQ-MDGIGGLRGWHWLFMLEGLP 190 LYLTYW P+ RAR+ LFM + A V P++ L++ M+G+G L W WLF+LEG+P Sbjct: 142 LYLTYWFPAQRRARVNGLFMTSFAIAGAVGGPIAGLIMSGMEGVGHLANWQWLFILEGIP 201 Query: 191 TVVLGLVCLSFLTDRPDQARWLSDEQRQWLTETMNKEAASRAAKQHIPLWKLFSQPHVWG 250 +++ G L++L +RP A+WLS+ ++ +T+ + E + A +H + VW Sbjct: 202 SILAGFAVLAWLPERPANAKWLSEAEKNAVTQVLAAE--NSAPGKHFSFKDACTNYRVWI 259 Query: 251 MALVCACASAAGSVLNVWQPQLLKSFGLS-VMQTGLVNSIPYLIASVLMVWWGRHSDRTG 309 A V C + + + W P ++K G+ +Q GL+++IP++ ++ M+W G HSD+TG Sbjct: 260 CAAVYFCVVSGNATIAFWAPSIIKEIGVKGNLQIGLISAIPFIAGTLAMIWNGFHSDKTG 319 Query: 310 ERRWHTAIPLMLIGGGLALASLAHSLAPVVLL-LTMVLIGAYSFKGPFWAMASSWLATGS 368 ERR H+AI ++ GL + A + L LT+ IG + FW++ ++LA + Sbjct: 320 ERRLHSAIATLIACIGLVATGMTLGNATLALCSLTLAAIGILAAFPVFWSIPGAFLAGTA 379 Query: 369 AAAGLAGINAVSNLIGGGLMVNVYGWVKDATGSYAMALLPMVGLSVVSVGILLFLSRK 426 AA G+A IN++ NL G + + G +K +TGS + L + L +V+ +++ RK Sbjct: 380 AAGGIALINSIGNL-AGFVAPYMIGALKTSTGSLSSGLYFVAALELVASFLVVVFIRK 436 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.138 0.442 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,132,952 Number of extensions: 40692 Number of successful extensions: 273 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 19 Length of query: 431 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 343 Effective length of database: 1,203,190 Effective search space: 412694170 Effective search space used: 412694170 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)