BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0141.p (309 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herb... 624 e-180 gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillu... 233 2e-62 gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane h... 127 2e-30 >gi|300313733|ref|YP_003777825.1| metal-dependent hydrolase [Herbaspirillum seropedicae SmR1] Length = 309 Score = 624 bits (1610), Expect = e-180, Method: Compositional matrix adjust. Identities = 309/309 (100%), Positives = 309/309 (100%) Query: 1 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH Sbjct: 1 MLDRRSFIAGAMLACAGVRSATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPH 60 Query: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD Sbjct: 61 WRGGRPDGATVADYRLLMAKLGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDD 120 Query: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA Sbjct: 121 DVDLPQLKEMDRLGVRGVRVNFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIA 180 Query: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP Sbjct: 181 QHEAVLAALPTQVVIDHLGRIPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPP 240 Query: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS Sbjct: 241 DYPDTSLLAKAYLQAAPQRTLWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVS 300 Query: 301 NPAALFGFA 309 NPAALFGFA Sbjct: 301 NPAALFGFA 309 >gi|300313739|ref|YP_003777831.1| amidohydrolase 2 [Herbaspirillum seropedicae SmR1] Length = 315 Score = 233 bits (593), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 129/288 (44%), Positives = 170/288 (59%), Gaps = 4/288 (1%) Query: 21 ATAQSVPNSAGSQPPRLKAPMLACDSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAK 80 A A + P+S+GSQ + P ACD H HIYD RF +P + ATVA YR L + Sbjct: 32 AQADAYPHSSGSQRAASRLPDDACDCHMHIYDPRFAWAPGAKLVHAP-ATVAMYRQLQQR 90 Query: 81 LGIARHVVVQPSTYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRV 140 LG R+VVV PS YG DN C LDAL Q G ARG+ V++D V +L+ + + GVRG+R Sbjct: 91 LGTTRNVVVTPSAYGVDNSCTLDALAQLGRSARGVAVVNDQVSDAELQRLHQAGVRGLRF 150 Query: 141 NFLSAQSWGVTTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGR 200 N VTTVE + A R+APLGWH+Q M + H A+LA LP +V DH R Sbjct: 151 NLAVG---SVTTVEMMEPLARRMAPLGWHLQANMPNEVLLAHRAMLARLPVPLVFDHFAR 207 Query: 201 IPQPDGLDHPGAQAVLRLLAKGNTWIKLSEPYADTLQGPPDYPDTSLLAKAYLQAAPQRT 260 IP HP V L+ + +KLS Y + +G PDY D + LA+A +Q AP + Sbjct: 208 IPLQADATHPVFDFVTGLMRERRASVKLSGAYLYSKRGAPDYDDVAPLARALVQLAPTQL 267 Query: 261 LWGSDWPHPTEKIKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGF 308 +WGSDWPHPTE+ KPDDA L D++ +W + + +LV+NPA L+ F Sbjct: 268 VWGSDWPHPTEQHKPDDARLLDVMISWLGSEEMVRTVLVANPARLYQF 315 >gi|300313878|ref|YP_003777970.1| metal-dependent transmembrane hydrolase [Herbaspirillum seropedicae SmR1] Length = 278 Score = 127 bits (318), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 95/280 (33%), Positives = 131/280 (46%), Gaps = 7/280 (2%) Query: 32 SQPPRLKAPMLACDSHHHIYDARFPVSPHWRGGRPDGATVADYRLLMAKLGIARHVVVQP 91 SQP A +L D+H H++ P++ R TV Y + G++ V+VQP Sbjct: 2 SQPCATPATVLRIDTHAHVFTRDLPLADVRRYAPTYDVTVEQYIARLDTHGMSHGVLVQP 61 Query: 92 STYGSDNRCLLDALTQFGAQARGIVVIDDDVDLPQLKEMDRLGVRGVRVNFL-SAQSWGV 150 S GSDNR LL AL ++ + RGI ++D DV QL + GV G+R N + A Sbjct: 62 SFLGSDNRYLLAALARYPQRLRGIAMVDADVSEQQLLALQAGGVVGIRFNLVGGAPVPDF 121 Query: 151 TTVERLRRTAERIAPLGWHVQVLMSGAQIAQHEAVLAALPTQVVIDHLGRIPQPDGLDHP 210 T V R T RIA LGW V++ + + L A+ +VV+DH GR+ G+D P Sbjct: 122 TGV--WRSTLARIAQLGWQVEIHREAVDLPRVLPPLLAMGLKVVVDHFGRVDAALGVDDP 179 Query: 211 GAQAVLRLLAKGNTWIKLSEPYAD--TLQGPPDYPDTSLLAKAYLQAAPQRTLWGSDWPH 268 G + +L A G W+KLS Y + QG L + + A QR LWGSDWPH Sbjct: 180 GFRYLLSSAAGGQVWVKLSASYRNGGAEQGQRFARQAWPLLREHFGA--QRLLWGSDWPH 237 Query: 269 PTEKIKPDDAVLFDLLAAWAPDPALRQQILVSNPAALFGF 308 + D + D R+ I A LFG Sbjct: 238 TQHESLTDYGHSWQQFTQLVSDEEQRRIITGQTAAQLFGI 277 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.136 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,046,116 Number of extensions: 46187 Number of successful extensions: 136 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 132 Number of HSP's successfully gapped: 3 Length of query: 309 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 224 Effective length of database: 1,217,395 Effective search space: 272696480 Effective search space used: 272696480 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 130 (54.7 bits)