BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0139.p (708 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase o... 1441 0.0 gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogen... 127 5e-30 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 119 6e-28 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 106 7e-24 gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein... 101 2e-22 >gi|300313731|ref|YP_003777823.1| sugar phosphate dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 708 Score = 1441 bits (3730), Expect = 0.0, Method: Compositional matrix adjust. Identities = 703/708 (99%), Positives = 705/708 (99%) Query: 1 MTIASPTGLAHPKAGQSRIASAWDEAYAATLDEPQLLLYRSNLLGADKEITNFGGGNTSA 60 MTIASPTGLAHPKAGQSRIASAWDEAYAATLDEPQLLLYRSNLLGADKEITNFGGGNTSA Sbjct: 1 MTIASPTGLAHPKAGQSRIASAWDEAYAATLDEPQLLLYRSNLLGADKEITNFGGGNTSA 60 Query: 61 KIAMPDPLTGQMVEVLWVKGSGGDLGSIKLDGFATLYMDKLEALRSRYRGLEHEDEMVGY 120 KIAMPDPLTGQ VEVLWVKGSGGDLGSIKLDGFATLYMDKLEALRSRYRGLEHEDEMVGY Sbjct: 61 KIAMPDPLTGQTVEVLWVKGSGGDLGSIKLDGFATLYMDKLEALRSRYRGLEHEDEMVGY 120 Query: 121 LPHCTFNLNARAASIDTPLHAYIPRRHVDHMHPDAVIAIAACKNSRDLTARIFEGELGWL 180 LPHCT+NLNARAASIDTPLHAYIPRRHVDHMHPDAVIAIAACKNSRDLTARIFEGELGWL Sbjct: 121 LPHCTYNLNARAASIDTPLHAYIPRRHVDHMHPDAVIAIAACKNSRDLTARIFEGELGWL 180 Query: 181 PWQRPGYDLGLKLEQLVRAQPHLKGIVLEGHGLFTWGDSARECYENTLNVIRRADAWLAA 240 PWQRPGYDLGLKLEQLVRAQPHLKGIVLEGHGLFTWGDSARECYENTLNVIRRADAWLAA Sbjct: 181 PWQRPGYDLGLKLEQLVRAQPHLKGIVLEGHGLFTWGDSARECYENTLNVIRRADAWLAA 240 Query: 241 NSRTPAFGGARYQALPADERAALAARIMPLLRGKISKGEYKLGHFDDSQAVLEFVCSNDL 300 NSRTPAFGGARYQALP ERAALAARIMPLLRGKISKGEYKLGHFDDSQAVL+FVCSNDL Sbjct: 241 NSRTPAFGGARYQALPKAERAALAARIMPLLRGKISKGEYKLGHFDDSQAVLDFVCSNDL 300 Query: 301 AALAALGTSCPDHFLRTKIRPLVLDFSPQQPDLAALEASLDAALEAYRQDYAAYYQRCAR 360 AALAALGTSCPDHFLRTKIRPLVLDFSPQQPDLAALEASLDAALEAYRQDYAAYYQRCAR Sbjct: 301 AALAALGTSCPDHFLRTKIRPLVLDFSPQQPDLAALEASLDAALEAYRQDYAAYYQRCAR 360 Query: 361 PNSPAMRDPNPVIYLIPGVGMLSFAKDKATARIAGEFYINAVNVMRGANGVDSYVGLPEQ 420 PNSPAMRDPNPVIYLIPGVGMLSFAKDKATARIAGEFYINAVNVMRGANGVDSYVGLPEQ Sbjct: 361 PNSPAMRDPNPVIYLIPGVGMLSFAKDKATARIAGEFYINAVNVMRGANGVDSYVGLPEQ 420 Query: 421 EAFDIEYWLLEEAKLQRMPKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQ 480 EAFDIEYWLLEEAKLQRMPKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQ Sbjct: 421 EAFDIEYWLLEEAKLQRMPKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQ 480 Query: 481 SALDSAQQSLLKHGGPDAIGVVRADITSEADVTTILNSVALRFGGIDLLVSNAGIASASP 540 SALDSAQQSLLKHGGPDAIGVVRADITSEADVTTILNSVALRFGGIDLLVSNAGIASASP Sbjct: 481 SALDSAQQSLLKHGGPDAIGVVRADITSEADVTTILNSVALRFGGIDLLVSNAGIASASP 540 Query: 541 LQDTSLEVWQRNLSVLATGYFLVSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCT 600 LQDTSLEVWQRNLSVLATGYFLVSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCT Sbjct: 541 LQDTSLEVWQRNLSVLATGYFLVSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCT 600 Query: 601 AKAAEIHLARCIALEGAPHGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEF 660 AKAAEIHLARCIALEGAPHGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEF Sbjct: 601 AKAAEIHLARCIALEGAPHGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEF 660 Query: 661 YRQRSMLKRSVLPEDIAEAVYFFASEKSAKSTGNILNVDAGNAGAFTR 708 YRQRSMLKRSVLPEDIAEAVYFFASEKSAKSTGNILNVDAGNAGAFTR Sbjct: 661 YRQRSMLKRSVLPEDIAEAVYFFASEKSAKSTGNILNVDAGNAGAFTR 708 >gi|300310578|ref|YP_003774670.1| beta-hydroxybutyrate dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 127 bits (318), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 87/263 (33%), Positives = 140/263 (53%), Gaps = 14/263 (5%) Query: 444 LAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDAIGVVR 503 L ++AL+TG GIG+ +A + +GA V++ D+ A + + ++K GG V Sbjct: 3 LKDKVALITGSASGIGKEIAVEYARQGAKVVIADLALEAAKATAEEIVKEGGTAM--AVA 60 Query: 504 ADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFLV 563 ++T E V + +GG+D+++SNAGI SP+ + SLE W++ L++ G FL Sbjct: 61 MNVTDETQVDKGVADAVNTYGGLDIMISNAGIQIISPVAELSLENWRKMLAIHLDGAFLT 120 Query: 564 SRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRV 623 +RAA +M+ QG GGSI+Y+ S + +AS + Y TAK + LA+ +A EGA IR Sbjct: 121 TRAAMKAMIKQGRGGSIIYMGSVHSHLASPLKAPYVTAKHGLLGLAKTVAKEGAKDKIRT 180 Query: 624 NVVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSV-----LPEDIAE 678 NV+ P +R + K E+A I +DV ++ MLK +V +D+A+ Sbjct: 181 NVICP-GFVRTPLVE--KQIPEQAKEFGISEEDV----IKKIMLKDTVDGEFTTTQDVAQ 233 Query: 679 AVYFFASEKSAKSTGNILNVDAG 701 F A+ + TG + V G Sbjct: 234 TAVFLAAFPTNALTGQSVVVSHG 256 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 119 bits (299), Expect = 6e-28, Method: Compositional matrix adjust. Identities = 85/255 (33%), Positives = 135/255 (52%), Gaps = 16/255 (6%) Query: 447 RIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDAIGVVRADI 506 R+ALVTGG GIG ++++QL A+G V++ DI+ A +L+ G A ++ D+ Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAA--PLQMDL 66 Query: 507 TSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVWQRNLSVLATGYFLVSRA 566 S + + +G D+LV+NAG+A D LE W + ++V TG L + Sbjct: 67 GSPESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQH 126 Query: 567 AFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRVNVV 626 A M+ Q G IV ++S +G+ A AG +AY T+KAA I L R +A+E A +GI VN V Sbjct: 127 AARLMVKQQWG-RIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSV 185 Query: 627 NPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPEDIAEAVYFFASE 686 P V + + + ++ E Y + ++R ++IA AV + A+E Sbjct: 186 APGPV-------------DTPMTQAMHSEQTRESYYRLVPMRRYGTTDEIASAVCYLAAE 232 Query: 687 KSAKSTGNILNVDAG 701 +SA TG+++ VD G Sbjct: 233 ESAYITGHVIPVDGG 247 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 106 bits (264), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 86/274 (31%), Positives = 127/274 (46%), Gaps = 28/274 (10%) Query: 430 LEEAKLQRMPKPKSLAGRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQS 489 L ++LQR G++ +VTG GIG+A AR+ EGA V+L D D +AL S Sbjct: 4 LNPSRLQR------FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAAALGKVFDS 57 Query: 490 LLKHGGPDAIGVVRADITSEADVTTILNSVALRFGGIDLLVSNAGIASASPLQDTSLEVW 549 L P+ D++ V +++ RFG +D+LVS+AG+ + + + S E W Sbjct: 58 L----PPERTAARETDVSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDW 113 Query: 550 QRNLSVLATGYFLVSRAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLA 609 R + G F +R A + + G IV VAS +GL A SAY +K A +L Sbjct: 114 HRVQATNVNGVFYGAREALPHL--EKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLT 171 Query: 610 RCIALEGAPHGIRVNVVNPDAVIRGSRIWDGKWKEERAASNKIEADD--VEEFYRQRSML 667 R +AL+ G+R+N V P A ADD + + + +R L Sbjct: 172 RAMALDFGRKGVRINAVCPSLT--------------HTAMTADMADDPPLLDKFAERIAL 217 Query: 668 KRSVLPEDIAEAVYFFASEKSAKSTGNILNVDAG 701 R P +IA + F AS ++ G L VD G Sbjct: 218 GRGADPLEIAAVITFLASPDASFVNGVNLPVDGG 251 >gi|300310595|ref|YP_003774687.1| 3-oxoacyl-(acyl-carrier protein) reductase [Herbaspirillum seropedicae SmR1] Length = 250 Score = 101 bits (252), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 83/259 (32%), Positives = 130/259 (50%), Gaps = 19/259 (7%) Query: 446 GRIALVTGGGGGIGQAVARQLLAEGACVMLTDIDQSALDSAQQSLLKHGGPDAIGVVRAD 505 GR A++TGG G G AVA +LL A V+L D+D+ AL A++ L G + V+ D Sbjct: 8 GRSAIITGGAQGFGYAVAERLLQGKAKVVLWDMDEKALAEARRKLESLGN---VETVKVD 64 Query: 506 ITSEADVTTILNSVALRFGGIDLLVSNAGIA-SASPLQDTSLEVWQRNLSVLATGYFLVS 564 I+S+A+V + + ID+LV +AGIA +P+ + S E W+R + + G F V+ Sbjct: 65 ISSQAEVQQAIEATEKLTQSIDILVHSAGIAGQNNPVAEYSPEEWRRVIDIDLNGAFYVN 124 Query: 565 RAAFSSMLTQGLGGSIVYVASKNGLVASAGASAYCTAKAAEIHLARCIALEGAPHGIRVN 624 + M+ Q G IV +AS G + ASAY AKA I L + + E A I VN Sbjct: 125 QVVVQRMIAQNYG-RIVNIASIAGKEGNPTASAYSAAKAGMIALTKSLGKETATKNIAVN 183 Query: 625 VVNPDAVIRGSRIWDGKWKEERAASNKIEADDVEEFYRQRSMLKRSVLPEDIAEAVYFFA 684 + P A +RI E+ I ++ + R V E++A V + Sbjct: 184 AITPAAA--QTRIL------EQCTQAHI------DYMLSKIPRARFVKVEELAAMVAWLV 229 Query: 685 SEKSAKSTGNILNVDAGNA 703 SE+++ +T ++ ++ G A Sbjct: 230 SEENSFTTASVFDLSGGRA 248 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,086,431 Number of extensions: 84103 Number of successful extensions: 389 Number of sequences better than 1.0e-08: 44 Number of HSP's gapped: 342 Number of HSP's successfully gapped: 44 Length of query: 708 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 616 Effective length of database: 1,184,250 Effective search space: 729498000 Effective search space used: 729498000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)