BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0124.p (420 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance ... 775 0.0 gi|300313899|ref|YP_003777991.1| RND family efflux transporter M... 62 1e-10 gi|300310689|ref|YP_003774781.1| RND family efflux transporter M... 60 5e-10 >gi|300313717|ref|YP_003777809.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 775 bits (2001), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/396 (96%), Positives = 389/396 (98%) Query: 25 MQAGTPAGAEKSSAHQEERGHKDNEHHGEEKGSEHKNSTEHAEGEHHDAEPSSEEGKIAL 84 MQAGTPAGAEKS AHQEE GHKDNEHHGEEKGSEHK++ EH EGEHHDAEPSSEEGKIAL Sbjct: 1 MQAGTPAGAEKSPAHQEEGGHKDNEHHGEEKGSEHKDAAEHGEGEHHDAEPSSEEGKIAL 60 Query: 85 NPEQIATAGIEVKTAGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVR 144 NPEQIATAGIEVKTAGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVR Sbjct: 61 NPEQIATAGIEVKTAGPMILNSSVQLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVR 120 Query: 145 KGQVLAVINSSAVSEQRSELLNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQVALRE 204 KGQVLAVINSSAVSEQRSE+LNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQ ALRE Sbjct: 121 KGQVLAVINSSAVSEQRSEMLNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQAALRE 180 Query: 205 AEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKHLGLGEAIKEDANAFLISDL 264 AEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKHLGLGEA+KEDANAFLISDL Sbjct: 181 AEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKHLGLGEAVKEDANAFLISDL 240 Query: 265 SSVWAEIIVSPKDMQFVRAGEQVTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPN 324 SSVWAEIIVSPKDMQFVR GE+VTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPN Sbjct: 241 SSVWAEIIVSPKDMQFVRTGEKVTVRATAFDASASGKISYVGSLIGEQSRTAKAHVILPN 300 Query: 325 PDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSVVFVRVPGGFAAQQVVTGRSNG 384 PDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSV+FVRVPGGFAAQQVVTGRSNG Sbjct: 301 PDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTVEEKSVIFVRVPGGFAAQQVVTGRSNG 360 Query: 385 KFVEIRSGLNAGAEYAATGSFAIKAELGKGSAEHSH 420 KFVEIRSGLNAG EYAATGSFAIKAELGKGSAEHSH Sbjct: 361 KFVEIRSGLNAGDEYAATGSFAIKAELGKGSAEHSH 396 >gi|300313899|ref|YP_003777991.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 406 Score = 61.6 bits (148), Expect = 1e-10, Method: Compositional matrix adjust. Identities = 67/276 (24%), Positives = 131/276 (47%), Gaps = 22/276 (7%) Query: 130 GIVESVSAKLGQKVRKGQVLAVINSSAVSEQRSELLNAEQRLA----LARTAYQREKQLW 185 G ++ + +G +V+KG++LA +++ + +Q ++ L A ++ A LAR++ R + L Sbjct: 104 GYLKRWTKDIGAEVKKGELLAELDTPEIDQQLTQALAAREQAASSMELARSSMARWEALR 163 Query: 186 EQKISAEQDYLQAQVALREAEVAVRNAQQKLRAIGATASSGGLNRYEIRAPFDGVVVEKH 245 ++ ++Q+ + + A +A + A +G G R I APF G++ ++ Sbjct: 164 KKDAVSQQELDERRSAFAQASANLAAADAN---VGRLRQVEGFKR--IVAPFSGIITRRN 218 Query: 246 LGLGEAIKEDAN--AFLISDLSSVWAEIIVSPKDMQFVRAGEQVTV-----RATAFDASA 298 + +G+ I A F++S + + V V+ G+QVTV R F+ Sbjct: 219 VDVGDLIDPGAGRALFVLSQTDPLRVYVNVPQSYANLVKPGQQVTVTQSELRGRKFE--- 275 Query: 299 SGKISYVGSLIGEQSRTAKAHVILPNPDDSWRPGLFVNVEVVSDEAQVPVAVSAEALQTV 358 GK++ I +R+ + V L N D + PG FV V + + + V+A+ L Sbjct: 276 -GKVARTAGAIDAATRSMQVEVTLSNADHALLPGAFVQVALPIKPSGA-LLVAADTLMFR 333 Query: 359 EEKSVVFVRVP-GGFAAQQVVTGRSNGKFVEIRSGL 393 + ++V P +++ GR+ G+ VE+ GL Sbjct: 334 RDGTLVAAVDPHNQVRLKKLRLGRNFGQTVEVLDGL 369 >gi|300310689|ref|YP_003774781.1| RND family efflux transporter MFP subunit [Herbaspirillum seropedicae SmR1] Length = 400 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 79/316 (25%), Positives = 132/316 (41%), Gaps = 28/316 (8%) Query: 109 QLPGEIRFNEDRTAHVVPQVAGIVESVSAKLGQKVRKGQVLAVINS----SAVSEQRSEL 164 +LPG R + + A V PQV GI+++ S + G V+ GQVL IN +A + L Sbjct: 56 ELPG--RTSPYQIADVRPQVNGIIKARSFREGSDVKAGQVLYQINPASYQAAYDSSVAAL 113 Query: 165 LNAEQRLALARTAYQREKQLWEQKISAEQDYLQAQVALREAEVAVRNAQQKLRAIGATAS 224 AE + AR R K+L K ++QDY A +L EAE V +A+ ++ S Sbjct: 114 AKAEASVRTARLKASRYKELVAIKAVSQQDYDDAVASLGEAEADVGSARANVQ-----TS 168 Query: 225 SGGLNRYEIRAPFDGVVVEKHLGLGEAIKEDANAFL------------ISDLSSVWAEII 272 L+ + AP G + + + G + + L ++ S+ W + Sbjct: 169 RINLDYARLDAPISGRIGKSSVTPGALVTANQTTALTTIQQLDPIYVDVTQSSTTWLNLK 228 Query: 273 VSPKDMQFVRAGEQVTVRATAFDA---SASGKISYVGSLIGEQSRTAKAHVILPNPDDSW 329 + + Q ++G VR + + G + + + + + I PNP Sbjct: 229 EALANGQLEKSGNGAKVRLVLENGRTYAQEGTLQFSDVTVNQDTGAITLRAIFPNPKTDL 288 Query: 330 RPGLFVNVEVVSDEAQVPVAVSAEAL-QTVEEKSVVFVRVPGGFAAQQ-VVTGRSNGKFV 387 PG++V + Q + V +A+ + K V FV G Q+ +VT R+ G Sbjct: 289 LPGMYVRAVLQEGVRQQGLLVPQQAVSRDGAGKPVAFVVNAQGVVEQRALVTDRAIGDQW 348 Query: 388 EIRSGLNAGAEYAATG 403 + SGL G + G Sbjct: 349 LVSSGLKEGDQLVVDG 364 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.126 0.341 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,162,978 Number of extensions: 44818 Number of successful extensions: 191 Number of sequences better than 1.0e-08: 9 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 9 Length of query: 420 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 332 Effective length of database: 1,203,190 Effective search space: 399459080 Effective search space used: 399459080 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 132 (55.5 bits)