BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0123.p (1073 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance ... 2147 0.0 gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump pr... 278 1e-75 gi|300313244|ref|YP_003777336.1| multidrug efflux system transme... 278 2e-75 gi|300309819|ref|YP_003773911.1| acriflavin resistance transmemb... 277 3e-75 gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump pr... 274 4e-74 >gi|300313716|ref|YP_003777808.1| cobalt-zinc-cadmium resistance cation efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1073 Score = 2147 bits (5564), Expect = 0.0, Method: Compositional matrix adjust. Identities = 1065/1073 (99%), Positives = 1069/1073 (99%) Query: 1 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE Sbjct: 1 MFERIIRFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLE 60 Query: 61 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQQARSQ 120 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQ ARSQ Sbjct: 61 AEQRISYPIETLMSGLPNLEQVRSLSRYGLSQVTVIFKDGTDIYFARQLVNERIQLARSQ 120 Query: 121 LPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKLQLRLVPG 180 LPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIK QLRLVPG Sbjct: 121 LPPGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKPQLRLVPG 180 Query: 181 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIRA 240 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIRA Sbjct: 181 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIERRGEQYLIRA 240 Query: 241 PGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLIGE 300 PGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLIGE Sbjct: 241 PGQVASIDDIGNIVLANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLIGE 300 Query: 301 NSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVLFV 360 NSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVLFV Sbjct: 301 NSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVLFV 360 Query: 361 FLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDFGIIIDGAVVIVENCVRR 420 FLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDFGIIIDGAVVIVENCVRR Sbjct: 361 FLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGALDFGIIIDGAVVIVENCVRR 420 Query: 421 LAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPM 480 LAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPM Sbjct: 421 LAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPM 480 Query: 481 AFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILMLWAKRLYEPMLKTIMENKPVV 540 AFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILMLWAKRLYEPMLKTIMENKPVV Sbjct: 481 AFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILMLWAKRLYEPMLKTIMENKPVV 540 Query: 541 LTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIEKTLKQK 600 LTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIEKTLKQK Sbjct: 541 LTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEMQKQIEKTLKQK 600 Query: 601 FPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSREQLLEAIQDEVAKIP 660 FPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSRE+LLEAIQDEVAKIP Sbjct: 601 FPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSREELLEAIQDEVAKIP 660 Query: 661 GNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLNDTAGKISAVLEKIPGATEVKIE 720 GNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLNDTA KISAVLEKIPGATEVKIE Sbjct: 661 GNSYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLNDTASKISAVLEKIPGATEVKIE 720 Query: 721 QTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQA 780 QTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQA Sbjct: 721 QTTGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQA 780 Query: 781 RTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSANVRG 840 RTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSANVRG Sbjct: 781 RTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSANVRG 840 Query: 841 RDLGGFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAM 900 RDLGGFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAM Sbjct: 841 RDLGGFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAM 900 Query: 901 FSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRS 960 FSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRS Sbjct: 901 FSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRS 960 Query: 961 LREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGIL 1020 LREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGIL Sbjct: 961 LREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGIL 1020 Query: 1021 SSTALTLLVLPLLYKLAHDKDEEDNPAGPLSRPPFWKFWLRQSSTVKGNHHGY 1073 SSTALTLLVLPLLYKLAHDKDEEDNPAGP SRPPFWKFW++QSST+KGNHHGY Sbjct: 1021 SSTALTLLVLPLLYKLAHDKDEEDNPAGPPSRPPFWKFWVKQSSTLKGNHHGY 1073 >gi|300313893|ref|YP_003777985.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1051 Score = 278 bits (712), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 273/1069 (25%), Positives = 493/1069 (46%), Gaps = 85/1069 (7%) Query: 7 RFAIEH---RWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQ 63 RF I+ W++ + ++ +G G+ S + L ++ PDI +V I + G S E Sbjct: 3 RFFIDRPIFAWVIAIIIM-LG--GMMSIRSLSLEQYPDIAPPKVNIKATYTGASAKTLED 59 Query: 64 RISYPIETLMSGLPNLEQVRSLSRYGLS-QVTVIFKDGTDIYFARQLVNERIQQARSQLP 122 ++ IE M GL NL+ + + S S V++ F GT+ A+ V ++QQ ++LP Sbjct: 60 SVTQVIEQQMKGLDNLQYMSASSSSAGSATVSLTFTAGTNPDVAQMQVQNKLQQVTARLP 119 Query: 123 PGIDPGMGPISTGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWVIKLQLRLVPGVT 182 + ++ +I + + DD R T+ D+ + + Q+ V GV Sbjct: 120 QAVQNNGVTVTKASTDILMVLSLTSDDPR------ITSIDIGDFISSTLTDQISRVEGVG 173 Query: 183 EINTIG-GFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYI----ERRGEQY- 236 ++ G +A + P+ KL Y + I AL+ N+ V AG I + G+Q Sbjct: 174 DVTAFGTNYAMRIWIDPA--KLQKYALMPSDITSALQAQNTEVSAGEIGALPAQPGQQIN 231 Query: 237 -LIRAPGQVASIDDIGNIVL-ANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTV 294 I A ++ + D+ NI++ ++V G +++ DVA VE+G E A+ +NG+ + Sbjct: 232 ATITARSKLTTPDEFRNIIVKSSVDGSVVKLSDVARVELGGESYQVASQQNGKPSSALAI 291 Query: 295 FMLIGENSRKVSQAVARKMEEINRSLPKGVEAVT--VYDRTVLVDKAIATVKKNLVEGAL 352 + G N+ + AV +K+ E+ P ++ T YD T V ++ V K L+E + Sbjct: 292 QLATGANALATADAVKKKLAELEPYFPASMKLKTNIAYDTTPFVRVSLEEVVKTLIEAIV 351 Query: 353 LVVAVLFVFLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGA--LDFGIIIDGA 410 LVV ++++FL NIRA I A+ +P+ +L TF + S N L++ L G+++D A Sbjct: 352 LVVLIMYLFLQNIRATFIPAITVPVVLLGTFGILAMFGYSINTLTMFGMVLAIGLLVDDA 411 Query: 411 VVIVENCVRRLAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALT 470 +V+VEN V RL + + TR + E+ A L+ +++ V++PM Sbjct: 412 IVVVEN-VERLMSEEKLSPKEATR-KSMQEITGA-------LVGIAMVLSAVFIPMAFFG 462 Query: 471 GVEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFIGEKVAE--KENILMLWAKRL--- 525 G G ++ + T+V+A+ ++++++T PA A + ++ W R Sbjct: 463 GSTGVIYRQFSITIVSAMALSVMVALTLTPALCATLLKPHAGGHVQQGRFFTWFNRSFDR 522 Query: 526 ----YEPMLKTIMENKPVVLTFAGALILVCGVVASRLGSEFVPSLNEGDMAIQALRIPGT 581 Y+ + +++ L + + V+ RL + F+P ++G + Q G Sbjct: 523 SATRYQGGVAGLLKRGKRGLLMYALIAVAMAVLFMRLPTSFLPEEDQGVLMAQIQLPTGA 582 Query: 582 SLSQSLEMQKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEP 641 + Q+ + K +++ K VE V A G A V + N + +I LK EQ Sbjct: 583 TAEQTATVVKTVQEYFMNKPESVESVLAIVG-AGVGGNSQ--NGARAFIRLKDWEQRTGA 639 Query: 642 KKSREQLLEAIQDEVAKIPGNSYEFSQPIQLR-------FN-EL--ISGVRSDVAVKVFG 691 +S +L+ +++KI G P +R F+ EL + GV D ++ Sbjct: 640 GQSASELVRRANADLSKIRGARVFLMNPPAVRGLGQSSGFDLELKDVGGVGHDALLQARD 699 Query: 692 DDMKVLNDTAGKISAVLEKIPGATEVKIEQTTGL---PMLTVEIDREKTARYGLNVSDIQ 748 +++ + +A+L + +TTGL P L V ID K + SDI Sbjct: 700 QFLQL-----ARSNALLANV---------RTTGLDDTPQLRVAIDDRKADALSIATSDIN 745 Query: 749 DTVATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVSYVPL 808 T+ATA+GG R + V+ R + + R + + A+++ VP Sbjct: 746 ATLATAMGGTYVNDFLSNGRVKKVYVQGDSAFRMQADDLNRWYV----RNAASQM--VPF 799 Query: 809 GEVASLTIAPGPNQISREDGKRRI-VVSANVRGRDLGGFVAEAERQLQSAVKVPAGYWTT 867 S GP+ + R +G +V G G +AE E+ + ++PAG Sbjct: 800 AAFMSSQWTYGPSLLERYNGVSSFEIVGEPAAGVSSGTAMAEVEKLV---AQLPAGIGFE 856 Query: 868 WGGQFENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGILALAM 927 W G + + + ++ +++L VF+ L A++ + V +P + G +L + Sbjct: 857 WTGSSYQERLSGNQAPMLYAISILFVFLCLAALYESWSVPFAVILAVPLGIIGTVLLTGI 916 Query: 928 RGIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTA 987 G+ + VG + G++ N ++++ F + L E G++L A E RLRP+LMT+ Sbjct: 917 FGMSNDVYFQVGLLTTVGLSAKNAILIVEFAKQLHESGKSLWEATLEAVQLRLRPILMTS 976 Query: 988 LVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLYKL 1036 L G +P+A+A+G G+ +R + T V+GG+LS+T L + +PL Y L Sbjct: 977 LAFMFGVLPLAVASGAGSASRRAIGTGVLGGMLSATLLGIFFVPLFYYL 1025 >gi|300313244|ref|YP_003777336.1| multidrug efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1033 Score = 278 bits (711), Expect = 2e-75, Method: Compositional matrix adjust. Identities = 291/1079 (26%), Positives = 493/1079 (45%), Gaps = 110/1079 (10%) Query: 7 RFAIEHRWLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQRIS 66 RF I+ + L + +GV + +LPI P + V IN + PG +P + Sbjct: 3 RFFIDRPIFAWVVALFIVLFGVIAMSQLPIARYPSVAPPTVSINATYPGATPQTMNDGVI 62 Query: 67 YPIETLMSGLPNLEQVRSLS-RYGLSQVTVIFKDGTDIYFARQLVNERIQQARSQLPPGI 125 IE +SG+ NL S + G +Q++V FK GTD A+ V +++ ++LP + Sbjct: 63 SLIERELSGVKNLLYFESSADTSGTAQISVTFKPGTDPELAQVDVQNKLKAIEARLPQTV 122 Query: 126 DPG----MGPISTGLGEIYLWTVESR-DDARKPDGTPYTATDLREIQDWVIKLQLRLVPG 180 S+ L + L +V+ R D+ R D Y A ++ E +LR + G Sbjct: 123 RQNGVTVESAASSFLLMVSLTSVDGRYDEVRLND---YLARNIVE--------ELRRMEG 171 Query: 181 VTEINTIGGFAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYIER----RGEQY 236 V + G + + KL +YG+T+ I A+ + N+ + G + RG+Q Sbjct: 172 VGRVQLFGA-EQAMRIWLDPFKLNAYGLTVTDISTAISQQNAQIAPGRLGDAPAVRGQQI 230 Query: 237 L--IRAPGQVASIDDIGNIVL-ANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGT 293 + GQ+ S IVL A G + + DVA VE+G + A ENG+ + Sbjct: 231 AMPLTVQGQLQSPAQFAAIVLKAKADGSRVLLGDVARVELGAQSYAFANRENGKPAGVAA 290 Query: 294 VFMLIGENSRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALL 353 V + G N+ + + A+ +MEE+ SLP+G+ Y+ V +I V + L+E +L Sbjct: 291 VQLSPGANAVRTAAAIQARMEELRPSLPQGMAFSLPYNTAPFVKISIQKVVQTLIEAMVL 350 Query: 354 VVAVLFVFLGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGA--LDFGIIIDGAV 411 V V+++FL N+R +I A+V P+A+L T M S N+L++ L GII+D A+ Sbjct: 351 VFLVMYLFLQNVRYTVIPAIVAPIALLGTLAVMQLAGYSVNVLTMFGMVLAIGIIVDDAI 410 Query: 412 VIVENCVRRLAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTG 471 V+VEN R +AH + R T A E +I L++ V++PM G Sbjct: 411 VVVENVERIMAH-EGLGPREAT--------IKAMGEITGAIIGITLVLTAVFIPMALAGG 461 Query: 472 VEGKMFTPMAFTVVAALIGAMILSVTFIPAAIALFIGEKVAEKENILMLWA------KRL 525 G ++ + + +++ + L+++ PA A + A + + RL Sbjct: 462 SVGVIYRQFSVAMAVSILFSAFLALSLTPALCATLLRPVPAGHHDKRGFFGAFNRAFNRL 521 Query: 526 ---YEPMLKTIMENKPVVLTFAGAL--ILVCGVVASRLGSEFVPSLNEGDMAIQALRIPG 580 YE L ++ ++ AL +LV G+ A L S F+P ++G + + ++P Sbjct: 522 TARYESALGRLIARGLRMMLVYAALSGLLVVGMQA--LPSAFLPEEDQGYF-MTSFQLPA 578 Query: 581 TSLSQ-SLEMQKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWP 639 + ++ + Q E+ LK + E + A PN + + LK W Sbjct: 579 DATTERTARAVDQFERYLKGR----EDIGANLSVLGFGFSGSGPNAAMAFTELK---DWK 631 Query: 640 EPKKSRE--------------------QLLEAIQDEVAKIPGNSYEFSQPIQLRFNELIS 679 E K S LL +E+ GNS F+ +Q R N+ Sbjct: 632 ERKGSTSAAEAMAAQAAMESGVDGMVMSLLPPAIEEL----GNSSGFAMRLQDRANQ--- 684 Query: 680 GVRSDVAVKVFGDDMKVLNDTAGKISAVL-EKIPGATEVKIEQTTGLPMLTVEIDREKTA 738 A+K + L + K+S V + +P V++E IDR K A Sbjct: 685 ---GYAALKAAEAQLLALAAKSRKLSGVYSDGLPAGASVQLE-----------IDRHKAA 730 Query: 739 RYGLNVSDIQDTVATAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQE 798 G++ S I DT++ A+G R ++++ AR LE + +L + Q Sbjct: 731 ALGVSFSSISDTLSAAMGSFYVNDFPNAGRMQQVILQAQASARMQLEDVMKLYVR--NQS 788 Query: 799 GATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSANVR-GRDLGGFVAEAERQLQSA 857 G VPL E+ + P Q+ R G + +S + G G +AE ER Q Sbjct: 789 GGM----VPLAELVKASWREAPTQLQRYQGFPAVRISGSAAPGVASGEAMAEMERLAQ-- 842 Query: 858 VKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFA 917 ++P G+ W GQ + + + +++ +++L+VF++L A++ + L V +P Sbjct: 843 -QLPPGFAVAWTGQSLQERLSAAQAPMLMALSMLVVFLVLAALYESWSIPLSVMLVVPLG 901 Query: 918 LTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAM 977 L G + A+ +R +P + VG I + G++ N ++++ F R LR EG L A + ++ Sbjct: 902 LVGAVAAVILRDMPNDVFFKVGMITVIGLSAKNAILIVEFARQLRSEGMGLVEAAIKASV 961 Query: 978 TRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLYKL 1036 RLRP+LMT+L +LG VP+ LA+G AE QR + T V GG++S+T L +L +P+ + L Sbjct: 962 LRLRPILMTSLAFALGVVPLMLASGASAETQRAIGTGVFGGMVSATVLAVLFVPVFFVL 1020 >gi|300309819|ref|YP_003773911.1| acriflavin resistance transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 1048 Score = 277 bits (709), Expect = 3e-75, Method: Compositional matrix adjust. Identities = 268/1063 (25%), Positives = 483/1063 (45%), Gaps = 97/1063 (9%) Query: 14 WLVLLAVLGMGAYGVYSYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQRISYPIETLM 73 W++ + ++ GA + LP+ P+I + + + PG + + ++ IE M Sbjct: 13 WVLAIFIMLAGALAITG---LPVSQYPNIAPPTIVVTATYPGATAQTLDDSVTSLIEQEM 69 Query: 74 SGLPNLEQVRSLSR-YGLSQVTVIFKDGTDIYFARQLVNERIQQARSQLPPGIDPGMGPI 132 +G L+ + S S+ G Q+T+ F T+ A V R+++ ++LP + + Sbjct: 70 NGADGLQYIESQSQANGQVQITITFSPETNPDLAAVDVQNRLKRVEARLPAAVTQQGVQV 129 Query: 133 STGLGEIYLWTVESRDDARKPDGTPYTATDLREIQDWV---IKLQLRLVPGVTEINTIGG 189 + L+ S D + D + D++ + +++ VPGV + G Sbjct: 130 TKSRSNFLLFIGLSSTDGK---------LDPVALGDYLSRNVLNEIKRVPGVGQAQLFGT 180 Query: 190 -FAKEYLVAPSAEKLASYGMTMDTIIKALEKNNSNVGAGYI---ERRGEQYL---IRAPG 242 A + P+ KL +T + A++ N+ V G + G Q + + G Sbjct: 181 ERAMRIWIDPN--KLIGLNLTPADVTAAIQAQNALVAGGTLGALPSPGTQQIAATVVVNG 238 Query: 243 QVASIDDIGNIVL-ANVKGVPIRIRDVAEVEIGRELRTGAATENGREVVLGTVFMLIGEN 301 Q+ S + GNI+L AN G +R+RDVA VE+G + + +G+ V + N Sbjct: 239 QLTSPEQFGNIILRANANGATVRLRDVARVEVGGQSYDTSGRLDGKPSSFIGVQLSPTAN 298 Query: 302 SRKVSQAVARKMEEINRSLPKGVEAVTVYDRTVLVDKAIATVKKNLVEGALLVVAVLFVF 361 + + AV +KMEE+ + P GV+ YD + V +I V K L+E +LV V+F+F Sbjct: 299 ALGTATAVHKKMEELAKYFPTGVKYTIPYDTSKFVKISIEEVVKTLIEAIVLVFLVMFLF 358 Query: 362 LGNIRAAIITAMVIPLAMLFTFTGMVSNKVSANLLSLGA--LDFGIIIDGAVVIVENCVR 419 L NIR II +V+P+A+L TF ++ S N+L++ L GI++D A+V+VEN V Sbjct: 359 LQNIRYTIIPTIVVPVALLGTFATLLLFGFSINVLTMFGMVLAIGILVDDAIVVVEN-VE 417 Query: 420 RLAHAQAHHGRTLTRSERFHEVFAAAKEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTP 479 R+ + TR A + LI L+++ V++PM G G ++ Sbjct: 418 RIMSEEGLSPADATRK--------AMGQISGALIGITLVLIAVFIPMAFFGGAVGAIYRQ 469 Query: 480 MAFTVVAALIGAMILSVTFIPAAIALFIG--EKVAEKENI-LMLWAKRLYEPMLKTI--- 533 + ++VA+++ +++++ T PA A + EK E W R + Sbjct: 470 FSLSMVASMVFSVLMAFTLTPALCATILKPVEKGHHHEKKGFFGWFNRKFNSTASGYQGI 529 Query: 534 ---MENKPVVLTFAGALILVC-GVVASRLGSEFVPSLNEGDMAIQALRIPGTSLSQSLEM 589 M NK LI+ C G++ RL + F+P+ ++G + PG S S++ + Sbjct: 530 IGGMLNKAGRYMIVYVLIVACVGLLYVRLPASFLPNEDQGYIITNVQLPPGASTSRTTAV 589 Query: 590 QKQIEKTLKQKFPEVERVFARTGTAEVASDPMPPNISDGYIMLKPQEQWPEPKKSREQ-- 647 KQIE+ K P VE A G + N + ++ P + W + KS+ Sbjct: 590 VKQIEE-YYLKDPAVEHAIAVLGYS------FSGNGQNAALVFTPLKDWSQRDKSQSADA 642 Query: 648 -------LLEAIQDEVA------KIP--GNSYEFSQPIQLRFNELISGVRSDVAVKVFGD 692 I+D + IP GN+ F+ +Q R +G+ V+ Sbjct: 643 IAGKAFGAFSQIKDAIVFPLNPPPIPELGNATGFTFRLQDR-----AGLGHTALVQARNQ 697 Query: 693 DMKVLNDTAGKISAVLEKIPGATEVKIEQTTGLPMLTVEIDREKTARYGLNVSDIQDTVA 752 + + + S VL V+ E P L ++IDR+K G+ S I ++ Sbjct: 698 ILGMASQ-----SKVLAG------VRPEGLEDAPQLQLDIDRDKANALGVPFSTINSVLS 746 Query: 753 TAIGGKEAGTMFQGDRRFGIVVRLPDQARTDLEAIRRLPIALPPQEGATRVSYVPLGEVA 812 TA+G R+ ++V+ + R E + L + + VP Sbjct: 747 TALGSSYVNDFPNLGRQQRVIVQADAKRRLQPEDLTNL------YARNSSGTMVPFSAFV 800 Query: 813 SLTIAPGPNQISREDGKRRIVVSANVR-GRDLGGFVAEAERQLQSAVKVPAGYWTTWGGQ 871 + GP Q+ R +G + ++ GR G +AE E + K+P G+ W GQ Sbjct: 801 TAKWINGPVQLIRYNGYPAMKIAGGAAPGRSTGEAMAEMESFV---AKLPPGFGFEWTGQ 857 Query: 872 FENLQSATQRLQIVIPVALLMVFVLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIP 931 +++ + + ++L+ VF++L A++ + L V +P + G +L + +R +P Sbjct: 858 SLEEKTSGNQAPALYALSLIAVFLVLAALYESTSIPLAVMLVVPLGILGALLGVTLRAMP 917 Query: 932 MSISAAVGFIALSGVAVLNGLVMISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTALVAS 991 + VG IA+ G++ N +++I F + L+ +G+ L A E R RP++MT+L Sbjct: 918 NDVYFKVGMIAVVGLSAKNAILIIEFAKDLQAQGKGLIEATLEAVHLRFRPIIMTSLAFI 977 Query: 992 LGFVPMALATGTGAEVQRPLATVVIGGILSSTALTLLVLPLLY 1034 LG +P+A+A+G G+ QR + T V+GG++++T L + ++P+ + Sbjct: 978 LGVLPLAIASGAGSASQRAIGTGVMGGMITATVLAVFLVPIFF 1020 >gi|300313900|ref|YP_003777992.1| cation/multidrug efflux pump protein [Herbaspirillum seropedicae SmR1] Length = 1063 Score = 274 bits (700), Expect = 4e-74, Method: Compositional matrix adjust. Identities = 273/1046 (26%), Positives = 492/1046 (47%), Gaps = 65/1046 (6%) Query: 30 SYQKLPIDAVPDITNVQVQINTSAPGYSPLEAEQRISYPIET-LMSGLPNLEQVRSLSRY 88 + + + D P+I V I + G S E QRI+ E L + + ++E + S S Sbjct: 28 TLKNMATDIFPEINIPVVSIIWNYNGLSAQEMGQRIAAQNERGLTTVVSDIEHIESQSLA 87 Query: 89 GLSQVTVIFKDGTDIYFARQLVNERIQQARSQLPPGIDPGMGPISTGLGEIYLWTVESRD 148 G+S + V F+ +I A V +Q QLPPGI P + I I + + + Sbjct: 88 GVSVIKVFFQPKANIQTAIAQVVASVQTQVRQLPPGITPPL-VIKYSASSIPVMQL-ALS 145 Query: 149 DARKPDGTPYTATDLREIQDWVIKLQLRLVPGVTEINTIGGFAKEYLVAPSAEKLASYGM 208 P+ T + A +Q ++ QL +PGV GG ++ V + L + G+ Sbjct: 146 SPTLPEQTVFDAA----VQ--TLRPQLVTIPGVAVPYPYGGKSRLISVDLDTQALQARGL 199 Query: 209 TMDTIIKALEKNNSNVGAGYIERRGEQYLIRAPGQVASIDDIGNIVLANVKGVPIRIRDV 268 ++ A+ N + +G + +Y ++ G ++ + + + G I ++DV Sbjct: 200 APIDVVNAINTQNLILPSGTAKFGATEYSVKINGSPEAVAGLNELPVRTTNGATIYLKDV 259 Query: 269 AEVEIGRELRTGAATENGREVVLGTVFMLIGENSRKVSQAVARKMEEINRSLPKGVEAVT 328 A V G +T ++G VL ++ G ++ + + + + ++LP+ ++ Sbjct: 260 ANVRDGFSPQTNVVRQDGARGVLLSILKNGGASTLDIVDNLRKLLPTAAQTLPEDIKITP 319 Query: 329 VYDRTVLVDKAIATVKKNLVEGALLVVAVLFVFLGNIRAAIITAMVIPLAMLFTFTGMVS 388 ++D++V V A+ V + A L A++ +FLGN R+ +I + IPL+++ + + Sbjct: 320 LFDQSVFVKAAVTGVISEALIAAGLTAAMVLLFLGNWRSTLIIGLTIPLSIMAAILILAA 379 Query: 389 NKVSANLLSLG--ALDFGIIIDGAVVIVENCVRRLAHAQAHHGRTLTRSERFHE-VFAAA 445 + NL++LG AL GI++D A+V +EN R L H G+ L H+ + A Sbjct: 380 LGETLNLMTLGGLALSVGILVDQAIVTIENIERHL-----HLGKPL------HDAIIVGA 428 Query: 446 KEARRPLIFGQLIIMIVYLPMFALTGVEGKMFTPMAFTVVAALIGAMILSVTFIPAAIAL 505 E P L I IV++PMF L+GV +F P+A VV A+ + +LS T +P + L Sbjct: 429 GEIGVPAFVATLCICIVFVPMFFLSGVARFLFVPLAEAVVFAMAASYLLSRTLVPTLVML 488 Query: 506 FIGE--KVAEKENILMLWAKRLYEPMLKTIMENKPVVLT------------FAGALILVC 551 +G +V + L+ R ++ + + +VL+ F G +L C Sbjct: 489 LMGSHGQVDSAKQSLLQRLYRAFDNRFERVRHGYTLVLSSLLAHRRRFALAFLGFCLLSC 548 Query: 552 GVVASRLGSEFVPSLNEGDMAIQALRIP-GTSLSQSLEMQKQIEKTLKQKFP--EVERVF 608 ++ LG +F P+++ G + + +R+P GT + ++ ++E+ ++ P E+E + Sbjct: 549 -LLYPFLGRDFFPNVDGGQIRLH-MRMPTGTRIEETARAADEVERVIRTLIPASELETIL 606 Query: 609 ARTGTAEVASDPMPPNIS-----DGYIMLKPQEQWPEPKKSREQLLEAIQDEVAK-IPGN 662 G + N DG I++ +E + E L+ ++ E+ + PG Sbjct: 607 DNLGVPNSGINLSYSNAGTIGTLDGEILMALKEG----HRPTEALVSMLRAELPRRFPGI 662 Query: 663 SYEFSQPIQLRFNELISGVRSDVAVKVFGDDMKVLNDTAGKISAVLEKIPGATEVKIEQT 722 + F QP + L G+ + + V+ G +M+ A +++ + +IPGA + + Q Sbjct: 663 EF-FFQPADIVTQILNFGLPAAIDVQFTGPNMEANARLAAELTKQIRQIPGAVDAHVHQR 721 Query: 723 TGLPMLTVEIDREKTARYGLNVSDIQDTVATAIGGK-EAGTMFQGDRRFGIV----VRLP 777 P L + +DR + ++GL+ S++ + A+ G + F + G+V V+ P Sbjct: 722 LDGPSLDLRMDRTRLQQFGLSASNVGQNLLIALAGSSQTQPAFWLNPNNGVVYNIAVQSP 781 Query: 778 DQARTDLEAIRRLPIALPPQEGATRVSYVPLGEVASLTIAPGPNQISREDGKRRIVVSAN 837 L+A+ +P++ P A+ LG + + SR + I V + Sbjct: 782 QYQVDSLDALLNIPVS--PGANASTTPPQLLGNLVDVHAGRQMAVASRYNIAPAIDVYVS 839 Query: 838 VRGRDLG---GFVAEAERQLQSAVKVPAGYWTTWGGQFENLQSATQRLQIVIPVALLMVF 894 V+G DL G V+ Q++ K+ G GQ E +QS+ L + + +A+++V+ Sbjct: 840 VQGTDLASVAGKVSALVEQIKP--KLARGSQVVIRGQVETMQSSFTGLGVGLAMAIVLVY 897 Query: 895 VLLFAMFSNVKDGLLVFTGIPFALTGGILALAMRGIPMSISAAVGFIALSGVAVLNGLVM 954 +L+ F + D ++ +P AL G L + G +S+ A G I GVA N +++ Sbjct: 898 LLVVVNFQSWIDAAIIIAALPAALAGIAWMLFITGTTLSVPALTGAIMTMGVATANSILI 957 Query: 955 ISFIRSLREEGRTLDAAIHEGAMTRLRPVLMTALVASLGFVPMALATGTGAEVQRPLATV 1014 ++F R REEG T AA E TR+RPVLMTAL +G +PMAL G GAE PL Sbjct: 958 VAFARQRREEGATPLAAALEAGSTRIRPVLMTALAMIIGMIPMALGLGEGAEQNAPLGRA 1017 Query: 1015 VIGGILSSTALTLLVLPLLYKLAHDK 1040 VIGG++ +T TL +P+++ H + Sbjct: 1018 VIGGLMLATVSTLFFVPVVFAGVHQR 1043 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.137 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 3,106,541 Number of extensions: 127836 Number of successful extensions: 583 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 504 Number of HSP's successfully gapped: 13 Length of query: 1073 Length of database: 1,619,870 Length adjustment: 95 Effective length of query: 978 Effective length of database: 1,170,045 Effective search space: 1144304010 Effective search space used: 1144304010 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 136 (57.0 bits)