BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0022.0112.p
         (261 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone hydro...    75   4e-15
gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamil...    59   4e-10
gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone hydr...    59   5e-10

>gi|300313276|ref|YP_003777368.1| 3-oxoadipate enol-lactone
           hydrolase/4-carboxymuconolactone decarboxylase
           [Herbaspirillum seropedicae SmR1]
          Length = 316

 Score = 75.5 bits (184), Expect = 4e-15,   Method: Compositional matrix adjust.
 Identities = 62/227 (27%), Positives = 104/227 (45%), Gaps = 4/227 (1%)

Query: 22  VLCIHGLGGSSNTWTPILPALAGR-KVIRLDLPGSGRSKLPAQALTIEAYVLTVRQVLKA 80
           V+  H LG     W  +   LA   +VI  D  G G S+      ++        ++L+ 
Sbjct: 77  VVLSHALGTDLMMWDGLANQLAADCRVIAYDHRGHGSSEKADGLYSMADLADDAARLLRE 136

Query: 81  LKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLFGPLVAPPEPARPAIRARAELARQGA 140
           L    V  +  SMG +V Q  A+ +P  V +LVL     A P+ AR A + R    R  A
Sbjct: 137 LDSGPVVWVGLSMGGMVGQELALRHPGLVRALVLANTTSAYPDAAREAWQQRIATVR--A 194

Query: 141 EKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCEALASAKSAP-IEE 199
           E ++ IADA++     +  +++    +   R+ ++     GY   C A+ +  +A  +  
Sbjct: 195 EGIEVIADAVMGRYFHEGFRSAQAATVARYRQRLVTTDAVGYVGCCHAVGTVDTAARLGS 254

Query: 200 IAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLEGCGHWTTFEK 246
           I VPTL++ G+ D   P A   A+   I G+++ V+ G  H +  E+
Sbjct: 255 IRVPTLVIAGELDQGTPVAMAQALVDGISGARLAVIAGASHVSAVEQ 301


>gi|300313233|ref|YP_003777325.1| alpha/beta hydrolase superfamily
           protein [Herbaspirillum seropedicae SmR1]
          Length = 289

 Score = 58.9 bits (141), Expect = 4e-10,   Method: Compositional matrix adjust.
 Identities = 45/126 (35%), Positives = 61/126 (48%), Gaps = 6/126 (4%)

Query: 15  GDGDGAPVLCIHGLGGSSNTWTPILPALA-GRKVIRLDLPGSGRSKLPAQA-LTIEAYVL 72
           GD  G P+L +HG+ G   T+  +   L  G + I LD  G G+S   A A    +AYV 
Sbjct: 21  GDPQGFPLLLLHGIRGYGETFGDLAQRLPPGYRCIALDQRGRGQSDWDAGANYYTDAYVA 80

Query: 73  TVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVL--FGPLVAPPEPARPAIR 130
            V  ++  L +   D+L HSMG I A  +A  +P  V  L++   GP      P   A R
Sbjct: 81  DVEALVARLNLRQFDLLGHSMGGINAIVYAARHPAKVRRLIIEDAGPGAFENSPG--ATR 138

Query: 131 ARAELA 136
            R ELA
Sbjct: 139 IRRELA 144


>gi|300310635|ref|YP_003774727.1| 3-ketoadipate enol-lactone
           hydrolase [Herbaspirillum seropedicae SmR1]
          Length = 261

 Score = 58.5 bits (140), Expect = 5e-10,   Method: Compositional matrix adjust.
 Identities = 64/230 (27%), Positives = 102/230 (44%), Gaps = 12/230 (5%)

Query: 12  EVEGDGDGAPVLCI-HGLGGSSNTWTPILPA-LAGRKVIRLDLPGSGRSKLPAQALTIEA 69
           ++EGD    PVL + + LG S + W P +P  L   +V+R D  G G+S++     +I  
Sbjct: 14  QLEGDAS-LPVLVLSNSLGTSLSMWDPQMPQWLQHFRVLRYDTRGHGQSEVTPGECSIAQ 72

Query: 70  YVLTVRQVLKALKIESVDILAHSMGTIVAQHFAVAYPDSVTSLVLF--GPLVAPPEPARP 127
               V  +L  L I        SMG       AV +P  +  L+L   G  +   E    
Sbjct: 73  LGSDVIALLDHLGIAQAHFCGLSMGGSTFMWLAVHHPQRINKLILCNTGAKIGTAE---- 128

Query: 128 AIRARAELARQGAEKVQEIADAIVAGATSKETKASNPMALTLVRESVMRQPPEGYAQSCE 187
           A  +R E  R+  E +  IA A+V+   + E   ++P  +  +   ++  P EGYA +C 
Sbjct: 129 AWNSRIETVRR--EGLGAIAGAVVSRWLTPEYAQAHPQQVQALTAMLLATPAEGYAAACA 186

Query: 188 ALASAK-SAPIEEIAVPTLLVTGDQDGVAPAANVNAMAQRIRGSKVLVLE 236
           A+        I  I  PTL++ G  D   P A+   M   I G++ +  +
Sbjct: 187 AVRDHDLREAIAGIRAPTLIIAGSGDVPTPPADAQFMRATIPGAQYVEFD 236


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.316    0.131    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 666,186
Number of extensions: 23935
Number of successful extensions: 151
Number of sequences better than 1.0e-08: 3
Number of HSP's gapped: 151
Number of HSP's successfully gapped: 3
Length of query: 261
Length of database: 1,619,870
Length adjustment: 83
Effective length of query: 178
Effective length of database: 1,226,865
Effective search space: 218381970
Effective search space used: 218381970
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 129 (54.3 bits)