BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0090.p (329 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313697|ref|YP_003777789.1| GDP-4-keto-6-deoxy-D-mannose-3,... 667 0.0 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 69 5e-13 gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 67 2e-12 >gi|300313697|ref|YP_003777789.1| GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [Herbaspirillum seropedicae SmR1] Length = 329 Score = 667 bits (1720), Expect = 0.0, Method: Compositional matrix adjust. Identities = 324/329 (98%), Positives = 325/329 (98%) Query: 1 MNFPIPAALPADMPSRDASIFVAGHAGLIGSAILRALQQAGYRKLITRTRAQLELRDKQQ 60 M PAALPADMPSRDASIFVAGHAGLIGSAILRALQQAGYRKLITRTRAQLELRDKQQ Sbjct: 1 MTLSTPAALPADMPSRDASIFVAGHAGLIGSAILRALQQAGYRKLITRTRAQLELRDKQQ 60 Query: 61 VDAFFEAERPDYVMLAAGKVGGILENQSFPADFINANLAISLNVLEAAHRCGVKRLVMFG 120 VDAFFEAERPDYVMLAAGKVGGILENQSFPADFINANLAISLNVLEAAHRCGVKRLVMFG Sbjct: 61 VDAFFEAERPDYVMLAAGKVGGILENQSFPADFINANLAISLNVLEAAHRCGVKRLVMFG 120 Query: 121 SSCMYPRETAQPMAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLIPNS 180 SSCMYPRETAQPMAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLIPNS Sbjct: 121 SSCMYPRETAQPMAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLIPNS 180 Query: 181 AYGPNDNFDPKSGHVLSSLMARFHQAKLDGAEAVTLWGSGTPRREFIHADDIAAAALLMM 240 AYGPNDNFDPKSGHVLSSLMARFHQAKLDGAEAVTLWGSGTPRREFIHADDIAAAALLMM Sbjct: 181 AYGPNDNFDPKSGHVLSSLMARFHQAKLDGAEAVTLWGSGTPRREFIHADDIAAAALLMM 240 Query: 241 REQLPALTLPLNVGSGSDISIRELAQAIAGVVGYEGRLEWDSSKPDGALRKLLDSSLMHQ 300 REQLPALTLPLNVGSGSDISIRELAQAIAGVVGYEGRLEWDSSKPDGALRKLLDSSLMHQ Sbjct: 241 REQLPALTLPLNVGSGSDISIRELAQAIAGVVGYEGRLEWDSSKPDGALRKLLDSSLMHQ 300 Query: 301 QGWRPQVGFAEGLRDTYAWYVQNKAGRAI 329 QGWRPQVGFAEGLRDTYAWYVQNKAGRA+ Sbjct: 301 QGWRPQVGFAEGLRDTYAWYVQNKAGRAV 329 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 68.9 bits (167), Expect = 5e-13, Method: Compositional matrix adjust. Identities = 77/331 (23%), Positives = 140/331 (42%), Gaps = 33/331 (9%) Query: 19 SIFVAGHAGLIGSAILRALQQAGYRKLI--------TRTRAQLELRD--KQQVDAFFEAE 68 I V G AGLIGS + L + ++++ +R Q L+D + D + Sbjct: 7 KIVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIM 66 Query: 69 RPDYVMLAAGKVGGI--------LENQSFPADFINANLAISLNVLEAAHRCGVKRLVMFG 120 + D + A G+ L+ +P + N+ + NV+EA GVKRLV Sbjct: 67 QTDILQSALEGADGVFHLAALWLLQCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVYSS 126 Query: 121 SSCMYPRETAQPMAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLIPNS 180 S+ +Y +PM E HP Y K+AG + +++ +YG ++ L + Sbjct: 127 SASVYGDAVREPMDED-----HPYNNKNFYGATKIAGETMLRSFHHRYGLN-YVGLRYMN 180 Query: 181 AYGPNDNFDPKSGHVLSSLMARFHQAKLDGAEAVTLWGSGTPRREFIHADDIAAAALLMM 240 YGP ++ G ++ +M +D E+ T+ G G+ +F+ +D A + M Sbjct: 181 VYGPRQDY---HGAYIAVIMKMLDA--IDKGESPTIMGDGSEAFDFVAVEDCGLANVCAM 235 Query: 241 REQLPALTLPLNVGSGSDISIRELAQAIAGVVGYEGRLEWDSSKPDGALRKLLDS--SLM 298 + + NVG+G S++ELA+ + + G + + + +R + Sbjct: 236 KAETA--DQYYNVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVRNRIGCPKKAS 293 Query: 299 HQQGWRPQVGFAEGLRDTYAWYVQNKAGRAI 329 + G+ + EGL+ W +KA A+ Sbjct: 294 SEIGFTATIDLREGLKRLIEWRANHKAEVAV 324 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 67.0 bits (162), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 75/347 (21%), Positives = 138/347 (39%), Gaps = 54/347 (15%) Query: 19 SIFVAGHAGLIGSAILRALQQAGYRKLI---------------------TRTRAQLELRD 57 +I V G AG IGS L +AG+ ++ T + + ++R+ Sbjct: 2 NILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 Query: 58 KQQVDAFFEAERPDYVMLAAGKVGGILENQSFPADFINANLAISLNVLEAAHRCGVKRLV 117 ++Q+++ + + V+ AG + + E+ P + + N+ ++ +LEA GVK +V Sbjct: 62 REQLESVLREFKCEAVIHFAG-LKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 Query: 118 MFGSSCMYPRETAQPMAETALLSGHPEPTSLPYAIAKLAGVQLCLAYNRQYGQQRFIPLI 177 S+ +Y P+ E HP S PY +K+ + Y R + + L Sbjct: 121 FSSSATVYGDPQYLPLDEA-----HPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLR 175 Query: 178 PNSAYGPNDN--FDPKSGHVLSSLMARFHQAKLDGAEAVTLWG------SGTPRREFIHA 229 + G +++ G + ++LM Q + E + +WG GT R+FIH Sbjct: 176 YFNPVGAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHV 235 Query: 230 DDIAAAALLMMREQLPALTLPLNVGSGSDISIRELAQAIAGVVGYE----------GRLE 279 D+A + ++ +N+G+G S+ ++ +A G G + Sbjct: 236 VDLARGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIA 295 Query: 280 WDSSKPDGALRKLLDSSLMHQQGWRPQVGFAEGLRDTYAWYVQNKAG 326 + P A RKL GW +G D++ W N G Sbjct: 296 SCYANPAEAKRKL---------GWEATLGLEAMCADSWKWQQMNPNG 333 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.135 0.402 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,048,343 Number of extensions: 42337 Number of successful extensions: 170 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 167 Number of HSP's successfully gapped: 3 Length of query: 329 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 243 Effective length of database: 1,212,660 Effective search space: 294676380 Effective search space used: 294676380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)