BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0086.p (338 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [H... 695 0.0 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 104 1e-23 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 101 1e-22 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 91 2e-19 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 82 7e-17 >gi|300313693|ref|YP_003777785.1| dTDP-glucose 4,6-dehydratase [Herbaspirillum seropedicae SmR1] Length = 338 Score = 695 bits (1794), Expect = 0.0, Method: Compositional matrix adjust. Identities = 336/338 (99%), Positives = 336/338 (99%) Query: 1 MLFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLVSVAGDARYLFSQTDIC 60 MLFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNL SVAGDARYLFSQTDIC Sbjct: 1 MLFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLASVAGDARYLFSQTDIC 60 Query: 61 DTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPA 120 DTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPA Sbjct: 61 DTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPA 120 Query: 121 AEQAAFRFLHISTDEVFGSLAAHAPPFTETSPYAPNSPYSASKAASDHLVRAFHSTYGLP 180 AEQAAFRFLHISTDEVFGSLAA APPFTETSPYAPNSPYSASKAASDHLVRAFHSTYGLP Sbjct: 121 AEQAAFRFLHISTDEVFGSLAADAPPFTETSPYAPNSPYSASKAASDHLVRAFHSTYGLP 180 Query: 181 VLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWLHVADHCRAIELVLA 240 VLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWLHVADHCRAIELVLA Sbjct: 181 VLITHCSNNYGPFHFPEKLIPLVITNALAGQPLPIYGDGQQVRDWLHVADHCRAIELVLA 240 Query: 241 RARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICFVQDRPGHDRRYAVD 300 RARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICFVQDRPGHDRRYAVD Sbjct: 241 RARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICFVQDRPGHDRRYAVD 300 Query: 301 TSKIQRELGWSPQHAFRQGLRQTVQWFLAARHASGARP 338 TSKIQRELGWSPQHAFRQGLRQTVQWFLAARHASGARP Sbjct: 301 TSKIQRELGWSPQHAFRQGLRQTVQWFLAARHASGARP 338 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 104 bits (259), Expect = 1e-23, Method: Compositional matrix adjust. Identities = 97/342 (28%), Positives = 143/342 (41%), Gaps = 39/342 (11%) Query: 3 FVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNL------VSVAGDARYLFSQ 56 +TG AGFIG+N + L + V +D + G NL V A A + F + Sbjct: 19 LITGVAGFIGSNLLARLLTL--GQQVTGLDNFS-TGRQANLEEVRAEVGAAAWANFRFIE 75 Query: 57 TDICDTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWS 116 DICD A R A V+H AA V RSI P NVNG +ML AA D Sbjct: 76 GDICDAATCLR--ACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARD--- 130 Query: 117 TLPAAEQAAFRFLHISTDEVFGSLAAH-APPFTETSPYAPNSPYSASKAASDHLVRAFHS 175 RF++ ++ +G H P E P SPY+ +K ++ F Sbjct: 131 ------AGVARFVYAASSSTYGD---HPGLPKVEEVIGKPLSPYAVTKYVNELYAEVFAR 181 Query: 176 TYGLPVLITHCSNNYGPFHFPE----KLIPLVITNALAGQPLPIYGDGQQVRDWLHVADH 231 YG+ + N +G P +IP +T + L I GDGQ RD+ ++ + Sbjct: 182 CYGMESIGLRYFNVFGRRQDPHGAYAAVIPQWVTAMIRNGELRINGDGQTSRDFCYIDN- 240 Query: 232 CRAIELVLARARPGES-----YNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRDQICF 286 ++ L A GE YN+ N + ++ + +LL + P Q + F Sbjct: 241 --VVQANLLAATAGEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPHLQEHRPSYVGF 298 Query: 287 VQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQWFL 328 R G R D K ++ LG++P H R GL Q + W++ Sbjct: 299 ---RAGDIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSWYI 337 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 101 bits (251), Expect = 1e-22, Method: Compositional matrix adjust. Identities = 98/346 (28%), Positives = 143/346 (41%), Gaps = 39/346 (11%) Query: 3 FVTGGAGFIGANYILHFLAAPHDEAVL---------NIDKLTYAGNPD---NLVSVAGDA 50 VTG AGFIG+N + L L N+D++ P+ N V GD Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 51 RYLFSQTDICDTAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSA 110 R L D C A + V+H AA V RSI P Q N++G +ML A Sbjct: 80 RKL----DDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVA 135 Query: 111 AYDYWSTLPAAEQAAFRFLHISTDEVFGSLAAHAPPFTETSPYAPNSPYSASKAASDHLV 170 A D RF++ ++ +G P E P SPY+ +K ++ Sbjct: 136 ARD---------ARVKRFVYAASSSTYGDHPG--LPKVEDRIGKPLSPYAVTKFVNELYA 184 Query: 171 RAFHSTYGLPVLITHCSNNYGPFHFPE----KLIPLVITNALAGQPLPIYGDGQQVRDWL 226 F TYGL + N +G P+ +IP I A+ G+ + I GDG+ RD+ Sbjct: 185 DVFARTYGLQTIGLRYFNIFGRRQNPDGAYAAVIPKWIAAAIKGEQIYINGDGETSRDFC 244 Query: 227 HVADHCRAIEL---VLARARPGESYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQYRD- 282 +V + +A L L + YN+ T E+ L D L P RD Sbjct: 245 YVDNAVQANLLAACTLQDEAANQVYNVAVGDRTTLKELYQFLRDNLSATFP----HLRDA 300 Query: 283 QICFVQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQWFL 328 Q F + R G R D K +R LG+ P H +GL++++ W++ Sbjct: 301 QPIFREFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNWYV 346 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 90.5 bits (223), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 95/347 (27%), Positives = 141/347 (40%), Gaps = 44/347 (12%) Query: 2 LFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLVSVAGDARYLFSQTDICD 61 + VTGGAG+IG++ + + A + VL+ + A D + ++G R F Q DI D Sbjct: 6 ILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQ-RPHFVQGDIRD 64 Query: 62 TAAIARLFAQHRPRAVVHCAAESHVDRSIAGPAVFIQTNVNGTFSMLSAAYDYWSTLPAA 121 A + +F HR AV+H A V S+A P + NV+G+ + A A Sbjct: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEA---------MA 115 Query: 122 EQAAFRFLHISTDEVFGSLAAHAPPFTETSPYAPNSPYSASKAASDHLVRAFHST----- 176 + S+ V+G A + P TE P + +PY SK + ++ H Sbjct: 116 AHGVKNIVFSSSATVYGDPA--SVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWR 173 Query: 177 -----YGLPVLITHCSNNYG--PFHFPEKLIPLVITNALAGQ-PLPIYG------DGQQV 222 Y PV H S G P P L+P + A + L +YG DG V Sbjct: 174 IALLRYFNPVG-AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGV 232 Query: 223 RDWLHVADHCRAIELVLARARPGE---SYNIGGNSEKTNLEVVLTLCDLLDELAPKAQGQ 279 RD++HV D L + G +YN+G + LE++ E A + Sbjct: 233 RDYIHVVDLALGHLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAF-----EQACGNKLP 287 Query: 280 YRDQICFVQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQW 326 Y+ V RPG T + +RELGW Q T +W Sbjct: 288 YQ----LVDRRPGDIACCYAATERAERELGWRAQRGIEAMCADTWRW 330 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 82.0 bits (201), Expect = 7e-17, Method: Compositional matrix adjust. Identities = 88/346 (25%), Positives = 145/346 (41%), Gaps = 60/346 (17%) Query: 2 LFVTGGAGFIGANYILHFLAAPHDEAVLNIDKLTYAGNPDNLVSVAGDARYLF------- 54 + V GGAG IG++ + H + E ++ + L G+ +NL + D R Sbjct: 8 IVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLR--GSRENLQNALKDPRVKIYDVGGDI 65 Query: 55 SQTDICDTAAIARLFAQHRPRAVVHCAA----ESHVDRSIAGPAVFIQTNVNGTFSMLSA 110 QTDI +A V H AA + H P NV GTF+++ A Sbjct: 66 MQTDILQSAL-------EGADGVFHLAALWLLQCH-----EYPRSAFDVNVRGTFNVMEA 113 Query: 111 AYDYWSTLPAAEQAAFRFLHISTDEVFGSLAAHAPPFTETSPYAPNSPYSASKAASDHLV 170 + R ++ S+ V+G A P E PY + Y A+K A + ++ Sbjct: 114 C---------VAKGVKRLVYSSSASVYGD--AVREPMDEDHPYNNKNFYGATKIAGETML 162 Query: 171 RAFHSTYGLPVLITHCSNNYGP---FHFPEKLIPLVITNAL-AGQPLPIYGDGQQVRDWL 226 R+FH YGL + N YGP +H + + + +A+ G+ I GDG + D++ Sbjct: 163 RSFHHRYGLNYVGLRYMNVYGPRQDYHGAYIAVIMKMLDAIDKGESPTIMGDGSEAFDFV 222 Query: 227 HVADHCRAIELVLARARPGESYNIGGNSEKTN------LEVVLTLCDLLDELAPKAQGQY 280 V D C + +A + Y G ++T+ L + +T CD AP Sbjct: 223 AVED-CGLANVCAMKAETADQYYNVGTGKRTSLKELAELLLEITGCDKPITFAP------ 275 Query: 281 RDQICFVQDRPGHDRRYAVDTSKIQRELGWSPQHAFRQGLRQTVQW 326 R Q V++R G + K E+G++ R+GL++ ++W Sbjct: 276 RSQATLVRNRIGCPK-------KASSEIGFTATIDLREGLKRLIEW 314 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.135 0.421 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,098,212 Number of extensions: 44544 Number of successful extensions: 151 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 138 Number of HSP's successfully gapped: 7 Length of query: 338 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 252 Effective length of database: 1,212,660 Effective search space: 305590320 Effective search space used: 305590320 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 131 (55.1 bits)