BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0085.p (336 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbas... 697 0.0 gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbas... 448 e-127 gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydra... 126 3e-30 gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herb... 100 2e-22 gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbas... 99 6e-22 >gi|300313692|ref|YP_003777784.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 336 Score = 697 bits (1798), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/336 (99%), Positives = 336/336 (100%) Query: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR 60 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR Sbjct: 1 MNILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIR 60 Query: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV Sbjct: 61 NREQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIV 120 Query: 121 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV 180 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV Sbjct: 121 FSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPV 180 Query: 181 GAHESGLIGEDPGGIPNNLIPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 GAHESGLIGEDPGGIPNNL+PFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR Sbjct: 181 GAHESGLIGEDPGGIPNNLMPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLAR 240 Query: 241 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN Sbjct: 241 GHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 Query: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFKA 336 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFKA Sbjct: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQMNPNGFKA 336 >gi|300311327|ref|YP_003775419.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 343 Score = 448 bits (1153), Expect = e-127, Method: Compositional matrix adjust. Identities = 211/329 (64%), Positives = 256/329 (77%), Gaps = 1/329 (0%) Query: 2 NILVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 ILVTGGAGYIGSHTCVEL+ AG+ V+V DN CNS + R+ QI+G+ V+GDIR+ Sbjct: 5 TILVTGGAGYIGSHTCVELIHAGYAVVVLDNLCNSRASVIDRIAQISGQRPHFVQGDIRD 64 Query: 62 REQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVF 121 R L+ + + + EAVIHFAGLKAVGESV QPL YYDNNV G+ L EAM A GVK IVF Sbjct: 65 RAVLDGIFKSHRIEAVIHFAGLKAVGESVAQPLRYYDNNVHGSNVLFEAMAAHGVKNIVF 124 Query: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVG 181 SSSATVYGDP +P+ E PLSA+NPYGRSK+++E +L D + A P W+IALLRYFNPVG Sbjct: 125 SSSATVYGDPASVPITEEFPLSATNPYGRSKLMVEQILGDLHVADPSWRIALLRYFNPVG 184 Query: 182 AHESGLIGEDPGGIPNNLIPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHVVDLARG 241 AHESGLIGEDP GIPNNL+PF+AQVA GRR L+++G DYPTPDGTGVRD+IHVVDLA G Sbjct: 185 AHESGLIGEDPSGIPNNLLPFIAQVADGRRAALSVYGSDYPTPDGTGVRDYIHVVDLALG 244 Query: 242 HVAALQALD-APQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIASCYAN 300 H+ L+ L+ P + NLGTG G SVL++L A+E+ACG LPY + +RRPGDIA CYA Sbjct: 245 HLKTLRKLEQGPGVYTYNLGTGRGNSVLEMLAAFEQACGNKLPYQLVDRRPGDIACCYAA 304 Query: 301 PAEAKRKLGWEATLGLEAMCADSWKWQQM 329 A+R+LGW A G+EAMCAD+W+WQ + Sbjct: 305 TERAERELGWRAQRGIEAMCADTWRWQAL 333 >gi|300313483|ref|YP_003777575.1| NAD_dependent epimerase/dehydratase [Herbaspirillum seropedicae SmR1] Length = 333 Score = 126 bits (316), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 106/329 (32%), Positives = 161/329 (48%), Gaps = 25/329 (7%) Query: 3 ILVTGGAGYIGSHTCVELLKAG-HEVIVFDNFCNSSPESVKRVQQITGKTLSLVKGDIRN 61 I+V GGAG IGSHT L+K E++++DNF S E+++ + + V GDI Sbjct: 8 IVVIGGAGLIGSHTVDHLVKEDVKEILIYDNFLRGSRENLQNALKDPRVKIYDVGGDIMQ 67 Query: 62 REQLESVLREFKCEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKTIVF 121 + L+S L + V H A L + + E P +D NV GT ++EA A GVK +V+ Sbjct: 68 TDILQSALE--GADGVFHLAALWLL-QCHEYPRSAFDVNVRGTFNVMEACVAKGVKRLVY 124 Query: 122 SSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLRYFNPVG 181 SSSA+VYGD P+DE HP + N YG +KI E MLR ++ + + LRY N G Sbjct: 125 SSSASVYGDAVREPMDEDHPYNNKNFYGATKIAGETMLRSFHHRYGLNYVG-LRYMNVYG 183 Query: 182 AHESGLIGEDPGGIPNNLIPFVAQVAIGRRERLNIWGGDYPT--PDGTGVRDFIHVVDLA 239 +D G A +A+ + I G+ PT DG+ DF+ V D Sbjct: 184 PR------QDYHG---------AYIAVIMKMLDAIDKGESPTIMGDGSEAFDFVAVEDCG 228 Query: 240 RGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRPGDIA-SCY 298 +V A++A A Q + N+GTG S+ ++ + + G P A R + + Sbjct: 229 LANVCAMKAETADQYY--NVGTGKRTSLKELAELLLEITGCDKPITFAPRSQATLVRNRI 286 Query: 299 ANPAEAKRKLGWEATLGLEAMCADSWKWQ 327 P +A ++G+ AT+ L +W+ Sbjct: 287 GCPKKASSEIGFTATIDLREGLKRLIEWR 315 >gi|300313479|ref|YP_003777571.1| UDP-galactose-4-epimerase [Herbaspirillum seropedicae SmR1] Length = 357 Score = 100 bits (248), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 96/346 (27%), Positives = 152/346 (43%), Gaps = 36/346 (10%) Query: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITG----KTLSLVKGDI 59 LVTG AG+IGS+ LLK V+ DNF ++ V+ + V+GDI Sbjct: 20 LVTGVAGFIGSNLLETLLKLNQRVVGLDNFSTGFQHNLDEVKALVTPEQWANFRFVEGDI 79 Query: 60 RNREQLESVLREFK-----CEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQAT 114 R + + + E V+H A L +V S+E P+ NN+ G + +L A + Sbjct: 80 RKLDDCRAAMHHEALGSNIVEYVLHQAALGSVPRSIEDPITTNQNNIDGFLNMLVAARDA 139 Query: 115 GVKTIVFSSSATVYGDPQYLPLDEAHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALL 174 VK V+++S++ YGD LP E +PY +K V E + R + I L Sbjct: 140 RVKRFVYAASSSTYGDHPGLPKVEDRIGKPLSPYAVTKFVNELYADVFARTYGLQTIG-L 198 Query: 175 RYFNPVGAHESGLIGEDPGGIPNNLIPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIH 234 RYFN G ++P G +IP AI + E++ I G DG RDF + Sbjct: 199 RYFNIFGRR------QNPDGAYAAVIPKWIAAAI-KGEQIYING------DGETSRDFCY 245 Query: 235 VVDLARGHVAA---LQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPY------A 285 V + + ++ A LQ A Q + N+ G ++ ++ + T P+ Sbjct: 246 VDNAVQANLLAACTLQDEAANQVY--NVAVGDRTTLKELYQFLRDNLSATFPHLRDAQPI 303 Query: 286 IAERRPGDIASCYANPAEAKRKLGWEATLGLEAMCADSWKW--QQM 329 E R GD+ A+ + +R LG+E + + +S W QQ+ Sbjct: 304 FREFRAGDVRHSLADIGKGRRLLGYEPSHRIGRGLKESMNWYVQQL 349 >gi|300312049|ref|YP_003776141.1| UDP-glucose 4-epimerase [Herbaspirillum seropedicae SmR1] Length = 347 Score = 98.6 bits (244), Expect = 6e-22, Method: Compositional matrix adjust. Identities = 97/338 (28%), Positives = 149/338 (44%), Gaps = 36/338 (10%) Query: 4 LVTGGAGYIGSHTCVELLKAGHEVIVFDNFCNSSPESVKRVQQITGK----TLSLVKGDI 59 L+TG AG+IGS+ LL G +V DNF +++ V+ G ++GDI Sbjct: 19 LITGVAGFIGSNLLARLLTLGQQVTGLDNFSTGRQANLEEVRAEVGAAAWANFRFIEGDI 78 Query: 60 RNREQLESVLREFK-CEAVIHFAGLKAVGESVEQPLMYYDNNVVGTVRLLEAMQATGVKT 118 + + LR + V+H A L +V S+E P+ NNV G + +L A + GV Sbjct: 79 CD---AATCLRACDGADYVLHQAALGSVSRSIEDPVRTNLNNVNGFLNMLVAARDAGVAR 135 Query: 119 IVFSSSATVYGDPQYLPLDE---AHPLSASNPYGRSKIVIEDMLRDYYRAHPDWKIALLR 175 V+++S++ YGD LP E PLS PY +K V E + R + I LR Sbjct: 136 FVYAASSSTYGDHPGLPKVEEVIGKPLS---PYAVTKYVNELYAEVFARCYGMESIG-LR 191 Query: 176 YFNPVGAHESGLIGEDPGGIPNNLIPFVAQVAIGRRERLNIWGGDYPTPDGTGVRDFIHV 235 YFN G +DP G +IP I R L I G DG RDF ++ Sbjct: 192 YFNVFGRR------QDPHGAYAAVIPQWVTAMI-RNGELRING------DGQTSRDFCYI 238 Query: 236 VDLARGHVAALQALDAPQCFAVNLGTGVGYSVLDVLKAYEKACGKTLPYAIAERRP---- 291 ++ + ++ A A + N+ S+ D+ + + P+ + E RP Sbjct: 239 DNVVQANLLAATAGEEAVNQVYNVALNDRTSLNDLYAMMRELLVQRFPH-LQEHRPSYVG 297 Query: 292 ---GDIASCYANPAEAKRKLGWEATLGLEAMCADSWKW 326 GDI A+ +A+++LG+ T L A + W Sbjct: 298 FRAGDIRDSQADIGKARQRLGYAPTHRLRAGLEQAMSW 335 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.137 0.420 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,157,849 Number of extensions: 51825 Number of successful extensions: 188 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 185 Number of HSP's successfully gapped: 8 Length of query: 336 Length of database: 1,619,870 Length adjustment: 86 Effective length of query: 250 Effective length of database: 1,212,660 Effective search space: 303165000 Effective search space used: 303165000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 131 (55.1 bits)