BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0076.p (255 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogena... 508 e-145 gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herb... 101 7e-23 gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herb... 80 2e-16 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 76 2e-15 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 72 5e-14 >gi|300313682|ref|YP_003777774.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 255 Score = 508 bits (1309), Expect = e-145, Method: Compositional matrix adjust. Identities = 252/255 (98%), Positives = 253/255 (99%) Query: 1 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIQALGRRAL 60 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREI+ LGRRAL Sbjct: 1 MTSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIETLGRRAL 60 Query: 61 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV 120 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV Sbjct: 61 AVQAELGDETQVRSLLPQAAPLGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV 120 Query: 121 LLAQALYEATPPGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 180 LLAQALYEATP GQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV Sbjct: 121 LLAQALYEATPQGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPKVRV 180 Query: 181 VGVAPGLTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 240 VGVAPGLTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG Sbjct: 181 VGVAPGLTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARAVTGTTLLVDGG 240 Query: 241 QHLLPLSRDVMFVAK 255 QHLLPLSRDVMFVAK Sbjct: 241 QHLLPLSRDVMFVAK 255 >gi|300311122|ref|YP_003775214.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 101 bits (251), Expect = 7e-23, Method: Compositional matrix adjust. Identities = 81/246 (32%), Positives = 127/246 (51%), Gaps = 14/246 (5%) Query: 9 IALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIQALGRRALAVQAELGD 68 + LVTG +R IG AL Q G+ VAV+Y +R AD +V +IQA G RA+AV ++ Sbjct: 7 VILVTGGSRGIGAATALLAGQRGYAVAVNYVTNRAAADAVVAQIQAAGGRAIAVGGDVAR 66 Query: 69 ETQVRSLLPQA-APLGRISCVVNNASLFDYDDA-ASFGNERLLRHMSANVGAPVLLAQAL 126 E V L LGR++ +VNNA + D+ ER+ R + ANV +L A+ Sbjct: 67 EEDVLQLFATVDGELGRVTALVNNAGVLDHQSRLEDMSAERINRVLIANVTGSLLCAREA 126 Query: 127 YEATPP---GQQAVVINL--LDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRV 180 + GQ ++NL + KL P +++ Y SK A+ + T LA+ +A + +RV Sbjct: 127 VKRMSTRHGGQGGAIVNLSSMAAKLGGPG-EYVDYAASKGAIDAFTIGLAKEVAAEGIRV 185 Query: 181 VGVAPGLTMVSGDQTEEQFGKAHQV---TPLGRGSTPEDIAAAVCYVASARA--VTGTTL 235 V PGL + + G+ ++ P+ RG + ++A A+ ++ S A TGT + Sbjct: 186 NAVRPGLIYTDIHASGGEAGRVDRLKDAVPMKRGGSAAEVANAILWLLSEEASYATGTFI 245 Query: 236 LVDGGQ 241 V GG+ Sbjct: 246 DVSGGR 251 >gi|300313186|ref|YP_003777278.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 257 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 79/248 (31%), Positives = 114/248 (45%), Gaps = 14/248 (5%) Query: 2 TSPSSAGIALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIQALGRRALA 61 TS + +A V G +R IG I LA G VA Y S ++A LV +QA G RALA Sbjct: 12 TSTLVSKVAFVQGGSRGIGAAIVQRLAAEGATVAFTYVSSPDKAQALVSSVQAKGGRALA 71 Query: 62 VQAELGDETQVRSLLPQAAP-LGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPV 120 +QA+ D ++ + AA GR+ +VNNA + F E L R ++ NV + Sbjct: 72 IQADSADAAALQQAIRLAAETFGRLDILVNNAGVLAMGPLDQFKLEDLDRTLAVNVRSVF 131 Query: 121 LLAQALYEATPPGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VR 179 + Q G + + I + P Y +SK+AL T LA+ L P+ + Sbjct: 132 VATQEAARYMGEGGRVISIGSTNADRI-PFAGGAVYAMSKSALVGLTKGLARDLGPRGIT 190 Query: 180 VVGVAPG-----LTMVSGDQTEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARA--VTG 232 V V PG + SGD G + L R E+IA+ V Y+A + +TG Sbjct: 191 VNNVQPGPVDTDMNPASGDFAASLTG----LMALPRYGKAEEIASFVAYLAGPESGYITG 246 Query: 233 TTLLVDGG 240 L++DGG Sbjct: 247 ANLMIDGG 254 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 76.3 bits (186), Expect = 2e-15, Method: Compositional matrix adjust. Identities = 76/244 (31%), Positives = 117/244 (47%), Gaps = 12/244 (4%) Query: 9 IALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIQALGRRA-LAVQAELG 67 +ALVTGA+R IGR IA LA+ G V + S A + + ALG A + Sbjct: 10 VALVTGASRGIGRAIATELARQGAKV-IGTATSESGAAAITEYLAALGPEAGRGAVLNVN 68 Query: 68 DETQVRSLLPQAAP-LGRISCVVNNASLFDYDDAASFGNERLLRHMSANVGAPVLLAQAL 126 D +L+ Q G +S +VNNA + A +E ++ N+ A L++A+ Sbjct: 69 DAEASVALVEQVQKDFGSLSILVNNAGITQDQLAMRMKDEEWDSVIATNLTAVGRLSRAV 128 Query: 127 YEATPPGQQAVVINLLDQKLYNPNPDFLSYTLSKAALHSATTLLAQALAPK-VRVVGVAP 185 + +IN+ NP ++Y +KA + + LA+ + + + V VAP Sbjct: 129 LRGMMKARTGRIINITSVVGEAGNPGQMNYAAAKAGVAGMSRALAREIGSRNITVNCVAP 188 Query: 186 GLTMVSGDQT----EEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARA--VTGTTLLVDG 239 G + D T E+Q Q PLGR E+IA AV ++AS +A +TGTTL V+G Sbjct: 189 GF--IDTDMTRALNEQQSAAILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNVNG 246 Query: 240 GQHL 243 G ++ Sbjct: 247 GMYM 250 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 71.6 bits (174), Expect = 5e-14, Method: Compositional matrix adjust. Identities = 70/245 (28%), Positives = 123/245 (50%), Gaps = 10/245 (4%) Query: 9 IALVTGAARRIGRHIALALAQSGWDVAVHYNRSREEADTLVREIQALGRRALAVQAELGD 68 + ++GA+ IG A A A G + VHYN S +EA+ + E++A G +++ + A++ D Sbjct: 8 VVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGADVRD 67 Query: 69 ETQVRSLLPQA-APLGRISCVVNNA-SLFDYDDAASFGNERLLRHMSANVGAPVLLAQAL 126 + + + + +A A +GRI ++NNA +L + +E ++ N + V +A+ Sbjct: 68 TSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAFTRAV 127 Query: 127 YEATPPGQQAVVINLLD-QKLYNPNPDFLSYTLSKAALHSATTLLAQA-LAPKVRVVGVA 184 +IN+ + P L Y SK + + T +A+ LA K+RV VA Sbjct: 128 IPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRVNAVA 187 Query: 185 PGLTMVSGDQ---TEEQFGKAHQVTPLGRGSTPEDIAAAVCYVASARA---VTGTTLLVD 238 PG+ M + T EQ + P+GR P++ + A Y+AS + VTG + V+ Sbjct: 188 PGVIMTPFQERFTTPEQLEGFRKTIPMGRIGEPDECSGAFLYLASEQMSGYVTGQIIDVN 247 Query: 239 GGQHL 243 GGQ++ Sbjct: 248 GGQYM 252 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.318 0.131 0.369 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 792,450 Number of extensions: 32304 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 186 Number of HSP's successfully gapped: 18 Length of query: 255 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 172 Effective length of database: 1,226,865 Effective search space: 211020780 Effective search space used: 211020780 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)