BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0072.p (444 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine ... 887 0.0 gi|300309980|ref|YP_003774072.1| two component transmembrane sen... 191 9e-50 gi|300312679|ref|YP_003776771.1| periplasmic sensor signal trans... 171 7e-44 gi|300311751|ref|YP_003775843.1| signal transduction histidine k... 170 2e-43 gi|300313819|ref|YP_003777911.1| two component sensor histidine ... 164 1e-41 >gi|300313679|ref|YP_003777771.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 444 Score = 887 bits (2292), Expect = 0.0, Method: Compositional matrix adjust. Identities = 441/444 (99%), Positives = 443/444 (99%) Query: 1 MNSVRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP 60 MNSVRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP Sbjct: 1 MNSVRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASLPRQAFSPISP 60 Query: 61 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST 120 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST Sbjct: 61 GQQFPELEDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWRVYSAQIGST 120 Query: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM Sbjct: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 Query: 181 NSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQL 240 NSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQL Sbjct: 181 NSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQL 240 Query: 241 AERAVDEEERRAAFADLKNGLERTTHLVHQLLTLARQEPDAMSFTQVDLRALLGSVVGDL 300 AERAVDEEERRAAFADLKNGLERTTHLVHQLLTLARQEPDAMSFTQVDLRALLGSVVGDL Sbjct: 241 AERAVDEEERRAAFADLKNGLERTTHLVHQLLTLARQEPDAMSFTQVDLRALLGSVVGDL 300 Query: 301 SSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLS 360 SSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLS Sbjct: 301 SSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGVDLS 360 Query: 361 TAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHGQEGSGSRAAFGSGLGLAIVRQIADLH 420 TAGYCTVVVQDSGPGIAESELPRVFDRFYRAQH QEGSGSRAAFGSGLGLAIVRQIADLH Sbjct: 361 TAGYCTVVVQDSGPGIAESELPRVFDRFYRAQHSQEGSGSRAAFGSGLGLAIVRQIADLH 420 Query: 421 RVPVQLRNTGHGLQARVVFPLAPA 444 RVPV+LRNTGHGLQARVVFPL+PA Sbjct: 421 RVPVELRNTGHGLQARVVFPLSPA 444 >gi|300309980|ref|YP_003774072.1| two component transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 390 Score = 191 bits (485), Expect = 9e-50, Method: Compositional matrix adjust. Identities = 127/336 (37%), Positives = 184/336 (54%), Gaps = 21/336 (6%) Query: 121 VVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRKAAAEVAARDM 180 VQV + R ++A ++ ++P ++ P+ L W A+SRGL+P+ + + AR Sbjct: 54 TVQVGETLEKRAQLANEIIKGVILPQFIILPVALALVWFALSRGLSPLSELQQRIRARRP 113 Query: 181 NSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTPLTALRLQTQL 240 + L P+ +P+EI PL +LND+LARL Q+ F+ADAAH+++TPL +R+Q++L Sbjct: 114 DDLSPIDSGQVPEEISPLVRSLNDMLARLSQTIAIQKRFIADAAHQMKTPLAGMRMQSEL 173 Query: 241 AERAVDEEERRAAFADLKNGLERTTHLVHQLLTLAR---QEPDAMSFTQVDLRALLGSVV 297 A R D+ + R + L E T LV+QLL+LAR Q+ + V+L L SVV Sbjct: 174 ALRQDDQVDVRRSLEQLAKSSETATRLVNQLLSLARAENQQSQSGGMIPVELNELARSVV 233 Query: 298 GDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNALRYTPAGGVVDVGV 357 D ++ IDLG +Q D P+ +TGN LR L +NL+DNAL YTP G V V Sbjct: 234 QDWVPMSFTQRIDLGF-EQPD--HPILITGNPVMLRELLSNLLDNALHYTPHDGNGRVTV 290 Query: 358 DLST---AGYCTVVVQDSGPGIAESELPRVFDRFYRAQHGQEGSGSRAAFGSGLGLAIVR 414 T G + V+D+GPGI +E VF+RFYR G +GS G GLGLAIVR Sbjct: 291 RARTDEGGGLAILEVEDNGPGIPVAERANVFERFYRI-LGTPSTGS----GLGLGLAIVR 345 Query: 415 QIADLHRVPVQLRNTGH-------GLQARVVFPLAP 443 +IA H +++ + G RV F + P Sbjct: 346 EIAQQHEAALEITSNPRSTDPKYPGCVFRVSFRMMP 381 >gi|300312679|ref|YP_003776771.1| periplasmic sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 459 Score = 171 bits (434), Expect = 7e-44, Method: Compositional matrix adjust. Identities = 147/472 (31%), Positives = 215/472 (45%), Gaps = 51/472 (10%) Query: 2 NSVRNKLLLWLGLGLFSIIVLAGVALYFQARGEANQIFDYQMRQIAASL-----PRQAFS 56 +S+R LL WL L + ++ Y+ A A FD + L RQ + Sbjct: 6 SSIRRDLLKWLIAPLLLVNLIGAGLTYWLAWLPAQHAFDQNLMDSTWGLYAQVRHRQERT 65 Query: 57 PISPGQQFPEL-----EDEIMMQIWDSRGAVIYHSHARGAMPRQAELGFANVSGPNGMWR 111 QQ ++ D + ++ G ++ +P A F +G R Sbjct: 66 TAELTQQAEQILRSNHSDTTFFAVRNTEGQIVVGDKGFPQLP--ASDVFDRPINYDGEMR 123 Query: 112 -------VYSAQIGSTVVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSW------ 158 ++ + V+ VA ++ R A + LL L G L++ Sbjct: 124 GEPVRIAAMQVRVKNGVIWVAVAETIRKRDRAHYTI-----LLSFLVLDGALTFASIFVV 178 Query: 159 -VAVSRGLAPVRKAAAEVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQR 217 VAV RGL P++ + RD L + G P E+QPL A+N+L+AR+ + Q+ Sbjct: 179 LVAVRRGLRPLKSLQRNLEQRDHGKLGAIDGRGAPVELQPLIVAMNELMARINKGEQAQQ 238 Query: 218 AFVADAAHELRTPLTALRLQTQ-LAERAVDEEERRAAFADLKNGLERTTHLVHQLLTLAR 276 F+AD AH+LRTPLT L+LQ + L ++ D+ + + A + + +ER QLL LAR Sbjct: 239 NFMADVAHQLRTPLTGLKLQLELLHDKHQDQPDTARSLAMMNSSVERMIRQSRQLLALAR 298 Query: 277 QEP---DAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADALR 333 EP ++ ++ L L+ V A+ IDLG D RP R+ G+ L Sbjct: 299 SEPGLFESRKLEELSLDKLVEESVQHFIEEADKKRIDLGF-----DLRPARLRGDRFLLS 353 Query: 334 ILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDRFYRAQH 393 L +NL+DNA+RY+P G V V L + V+DSGPGIA +FDRFYRA Sbjct: 354 DLIDNLIDNAVRYSPPHGTVTVRC-LEEQDATVLSVEDSGPGIAPEHRELIFDRFYRA-- 410 Query: 394 GQEGSGSRAAFGSGLGLAIVRQIADLH--RVPVQLRNTGHGLQARVVFPLAP 443 + GSGLGLAIVR+IA H + V + G G V FPLAP Sbjct: 411 ------NDKVAGSGLGLAIVREIAHDHGGVISVDCKEQGQGTIFTVRFPLAP 456 >gi|300311751|ref|YP_003775843.1| signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 446 Score = 170 bits (431), Expect = 2e-43, Method: Compositional matrix adjust. Identities = 138/359 (38%), Positives = 190/359 (52%), Gaps = 25/359 (6%) Query: 92 MPRQAEL--GFANVSGPNGMWRVY-SAQIGSTVVQVAQPQSARDEIAAQMAVKTVVPLLL 148 +P A+L GF + + +RVY A + +AQ R IA + A + + P ++ Sbjct: 101 LPLPADLAEGFHTLLLHHEYFRVYVQALPNGKRLALAQETDLRQHIAQRSAWRALWPFVV 160 Query: 149 LFPLIGVLSWVAVSRGLAPVRKAAAEVAARDMNSLQPLADVGMPQEIQPLTHALNDLLAR 208 L PL+ + + R AP++ E+ +R + LQP+A+ +P+E+Q A+N LLAR Sbjct: 161 LAPLLLLAVQRIIKRLFAPLQALRQEIDSRGEDDLQPIAERLLPREVQAFVAAINGLLAR 220 Query: 209 LKHATETQRAFVADAAHELRTPLTALRLQTQLAERAVDEEERRAAFADLKNGLERTTHLV 268 ++ QR FVADAAHELRTPLTAL LQ + RA E R L+ GL+R LV Sbjct: 221 VEAGMAAQRRFVADAAHELRTPLTALSLQAERLGRAPMSETARERLTRLETGLQRARQLV 280 Query: 269 HQLLTLAR-QEPDAMSFTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTG 327 QLL LAR Q P + L +L V+ +L +A+ IDLGL D R V Sbjct: 281 TQLLALARAQTPAGAALQPCSLHQVLREVIEELLPLAQCKQIDLGL----DGERDACVLT 336 Query: 328 NADALRILFNNLVDNALRYTPAGGVVDVGVDLSTAGYCTVVVQDSGPGIAESELPRVFDR 387 L L NL+DNA+ YTPAGG V + ++ A + + + D+GPGIA SE RVF Sbjct: 337 QPQELAQLLRNLIDNAIAYTPAGGAVSLRME-RHAQWVALEICDTGPGIAASERERVFQP 395 Query: 388 FYRAQHGQEGSGSRAAFGSGLGLAIVRQIADLHRVPVQLRNTG------HGLQARVVFP 440 FYR + SG GSGLGLAIV+ IA R+ V LR GL+ R++ P Sbjct: 396 FYRI----DPSGQ----GSGLGLAIVQSIA--RRLGVTLRLEAADPAQQSGLRVRLLIP 444 >gi|300313819|ref|YP_003777911.1| two component sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 485 Score = 164 bits (416), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 130/345 (37%), Positives = 180/345 (52%), Gaps = 28/345 (8%) Query: 112 VYSAQI-GSTVVQVAQPQSARDEIAAQMAVKTVVPLLLLFPLIGVLSWVAVSRGLAPVRK 170 VY I G ++QV + R+ + ++ T+ L + +L W AV L P+ + Sbjct: 144 VYDDSIRGIALIQVGETMEGRNGMTRKILFDTLWRQGTLVTVAALLVWFAVRFVLRPLMQ 203 Query: 171 AAAEVAARDMNSLQPLADVGMPQEIQPLTHALNDLLARLKHATETQRAFVADAAHELRTP 230 EV +R L + +E++PL A+N + RL+ QR F+ADA+H+LRTP Sbjct: 204 LKGEVESRAPTDLSGFDTALVHKEMRPLVQAMNGYMGRLQALISAQRRFIADASHQLRTP 263 Query: 231 LTALRLQTQLAERAVDEEERRAA-FADLKNGLERTT----HLVHQLLTLARQEPDAMS-- 283 LT L+ Q++L R + E+ AA DL G+ RTT HL ++LLTLAR E A Sbjct: 264 LTVLKTQSELTLRELRREQVDAAELRDLVEGMARTTDAAVHLANRLLTLARAEHGAAEGG 323 Query: 284 FTQVDLRALLGSVVGDLSSVAEHGGIDLGLSDQGDDGRPLRVTGNADALRILFNNLVDNA 343 + L V +LS A I L ++GD + + NA L L NL+DNA Sbjct: 324 TMPLSLSDAARQVALELSQQAVSREIGLAFEEEGD----VSIEANALLLHELMVNLLDNA 379 Query: 344 LRYTPAGGVVDVGVDL-STAGYCTVV---VQDSGPGIAESELPRVFDRFYRA---QHGQE 396 +RYTPAGG V + V S+ G TV V+DSGPGI +E +VF+ FYRA QH Sbjct: 380 IRYTPAGGHVTLRVRAQSSPGQPTVALLEVEDSGPGIPAAEREKVFEPFYRAGATQHVNP 439 Query: 397 GSGSRAAFGSGLGLAIVRQIADLHRVPVQLR--NTGHGLQARVVF 439 G G+GLGL+IVR+IA +HR V L G GL R+VF Sbjct: 440 G-------GTGLGLSIVREIAAMHRASVTLDAPAQGSGLVVRIVF 477 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.135 0.390 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,289,657 Number of extensions: 54394 Number of successful extensions: 323 Number of sequences better than 1.0e-08: 37 Number of HSP's gapped: 234 Number of HSP's successfully gapped: 37 Length of query: 444 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 356 Effective length of database: 1,203,190 Effective search space: 428335640 Effective search space used: 428335640 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 132 (55.5 bits)