BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0070.p (609 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium... 1045 0.0 gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium... 239 4e-64 gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux trans... 195 8e-51 gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, m... 106 5e-24 >gi|300313677|ref|YP_003777769.1| glutathione-regulated potassium-efflux system transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 606 Score = 1045 bits (2703), Expect = 0.0, Method: Compositional matrix adjust. Identities = 538/544 (98%), Positives = 538/544 (98%) Query: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF Sbjct: 1 MDEQLLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEF 60 Query: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAAGVPWRIGLIAALG 120 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAA VPWRIGLI ALG Sbjct: 61 GVVLLLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAASVPWRIGLIGALG 120 Query: 121 MSLSSTAIALATLGERKLTATPAGSAARPILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 MSLSSTAIALATLGERKLTATPAGSAA ILLFQDIAAIPMMAAVPLLGVAASQGSGQGW Sbjct: 121 MSLSSTAIALATLGERKLTATPAGSAAFSILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 Query: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA Sbjct: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 Query: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG 300 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG Sbjct: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLG 300 Query: 301 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV 360 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV Sbjct: 301 FLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVV 360 Query: 361 VVALSMLVTPLLLLVHDRLLVRWMEGGKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAA 420 VVALSMLVTPLLLLVHDRLLVRWMEGGKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAA Sbjct: 361 VVALSMLVTPLLLLVHDRLLVRWMEGGKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAA 420 Query: 421 NGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM 480 NGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM Sbjct: 421 NGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEM 480 Query: 481 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA 540 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA Sbjct: 481 VDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESALHMGRQVLQQLDFGAYRA 540 Query: 541 RARA 544 R A Sbjct: 541 REAA 544 >gi|300313254|ref|YP_003777346.1| glutathione-regulated potassium-efflux system K+/H+ antiporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 663 Score = 239 bits (610), Expect = 4e-64, Method: Compositional matrix adjust. Identities = 185/550 (33%), Positives = 280/550 (50%), Gaps = 27/550 (4%) Query: 5 LLIALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVL 64 L + L L AAV V + + L +LGYL+ GI+IGP GLG + + EFGVV Sbjct: 5 LELTLFLLAAAVLGVVAFRMMQLPPMLGYLVVGIIIGPHGLGFAEDNETTHALGEFGVVF 64 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAA--------GVPWRIGLI 116 L+F IGLE +L A+R +FG G QVA A + A + W+ Sbjct: 65 LMFSIGLEFSLAKLSAMRHIVFGLGVAQVATTIAATMVLGWGAATFLPQFFNISWQAAFA 124 Query: 117 AALGMSLSSTAIALATLGERKLTATPAGSAARPILLFQDIAAIPMMAAVPLLGVAASQGS 176 +++SSTAI L ER T G +LLFQD+A +P++ VP A + S Sbjct: 125 LGGALTMSSTAIVSKLLTERLELETEHGRRILGVLLFQDLALVPLLIVVP----ALANES 180 Query: 177 GQ-----GWLHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLL 231 G GW AG+ +V+ALL+F G LMR RI+ K +E+F LL+ + + Sbjct: 181 GNIMVTLGW-AAGKALVVLALLLFFGSKLMRGWFRIVVKRRSQELFMLNLLLITLGAAWI 239 Query: 232 MQSVGMSMALGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQP 291 + G+S+ALG F+AG+L++++E++H +E D++ F+ +LLGLFFI +GM ++ Sbjct: 240 TEKAGLSLALGAFIAGMLISETEFKHQVEEDIKSFRDVLLGLFFITIGMLLNVRTVIDHW 299 Query: 292 WRILGMVLGFLAIKTCVMWALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLT 351 + +L ++ G + +K ++ AL R FG G L L+Q GEF F++ A K++ Sbjct: 300 FLVLLLLTGPVLLKFGLIAALARLFGSTPGVALRVGLGLAQAGEFGFVLLSQAGGLKLVD 359 Query: 352 PETSSLLVVVVALSMLVTPLLLLVHDRLLVR-----WMEGG---KRRPEDKIDPQDHAVI 403 P +++ + LSML TP +L D ++++ WM + + Q H VI Sbjct: 360 PMLIQVILAAMVLSMLATPFILAKSDAIVMKFSANEWMLQSLALTQLATRTMGVQKH-VI 418 Query: 404 IAGFGRFGQIIGRLLAANGIGATVLDHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADK 463 IAGFGR GQ + RLL GI L+ DP+ + R G V YGDA R + L AG + Sbjct: 419 IAGFGRSGQSLARLLEEEGIAYHALEMDPELVQDARNGGANVSYGDAARRESLVAAGIHR 478 Query: 464 ARVLVIAIDDAQAALEMVDKVREEFPHLQIAARARNVTHYYDLMDRGVTVLERETFESAL 523 A LV+ +AL+++ E P L + R+ + L+ G T + E E +L Sbjct: 479 AAALVVTYASTPSALKVLHHAAELEPELPVIVRSHDDADLEKLLAAGATEVVPEALEGSL 538 Query: 524 HMGRQVLQQL 533 + L L Sbjct: 539 MLASHALVML 548 >gi|300311286|ref|YP_003775378.1| Kef-type potassium efflux transporter NAD-binding protein [Herbaspirillum seropedicae SmR1] Length = 546 Score = 195 bits (496), Expect = 8e-51, Method: Compositional matrix adjust. Identities = 167/520 (32%), Positives = 263/520 (50%), Gaps = 17/520 (3%) Query: 21 IAKRLGLGAVLGYLLAGIVIGPWGLGLITEVKHILEFSEFGVVLLLFLIGLELDPKRLWA 80 +A RL + ++GYL+AGI IGP+ G + + E +E GV+LL+F +GL K L + Sbjct: 24 LAHRLKMPPLVGYLVAGIAIGPFTPGFVGDADLAQELAEIGVILLMFGVGLHFSLKDLLS 83 Query: 81 LRRSIFGWGSIQVAAVSLALLAAALAAGVPWRIGLIAALGMSLSSTAIALATLGERKLTA 140 ++ Q+ +L +A G P G + L +S++ST + L L ER+L Sbjct: 84 VKNIAIPGAVAQITIATLLGMALGWLLGWPVGEGFLFGLALSVASTVVLLKALQERRLVE 143 Query: 141 TPAGSAARPILLFQDIAAIPMMAAVPLL-GVAASQG---SGQGWLHA-----GQVALVIA 191 T G A L+ +DIA + + +P L G+ +G SG L G+V A Sbjct: 144 TQRGRIAVGWLIVEDIAMVLALVLIPALSGILGGKGEALSGNAVLLTLGITLGKVVAFAA 203 Query: 192 LLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSV-GMSMALGTFLAGVLL 250 +++ GR ++ IL IA T RE+F L + + L V G+S ALG F AG++L Sbjct: 204 VMLIVGRRVIPWILERIADTGSRELFRLAVLAIALGFALGSAYVFGVSFALGAFFAGMIL 263 Query: 251 ADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVFFAQPWRILGMVLGFLAIKTCVMW 310 ++SE H + P + LFF++VGM D + +P +L VL + K+ Sbjct: 264 SESELSHRAAEESLPLRDAFSVLFFVSVGMLFDPSIVAREPLLVLATVLIIVVGKSLGAL 323 Query: 311 ALGRCFGIPRGQQLFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSLLVVVVALSMLVTP 370 + R FG P G L + L+Q GEF+FI+ ++ +L+P L++ LS+L+ P Sbjct: 324 LIVRAFGYPNGTALTISASLAQIGEFSFILATLGLSLDLLSPMARDLILGGAILSILLNP 383 Query: 371 LLLLVHDRLLVRWMEG--GKRRPEDKIDPQDHAVIIAGFGRFGQIIGRLLAANGIGATVL 428 LL + DR R + G P+ K++ QDH V++ GFGR G+ IG L A G V+ Sbjct: 384 LLFSLLDRYEARQPKAPQGGDEPQ-KVELQDH-VVLVGFGRVGRGIGEQLRAQGKPFVVI 441 Query: 429 DHDPDQIDFLRKFGFKVFYGDATRIDLLRTAGADKARVLVIAIDDAQAALEMVDKVREEF 488 + + ++ LR G YG+A + +LL+ A D+AR L++AI + A ++++ E Sbjct: 442 EAQLENLEALRAAGVPALYGNAAQSELLQAAAIDRARWLLVAIPEVFEAGQVIENALELN 501 Query: 489 PHLQIAARARNVTHYYDLMDRG---VTVLERETFESALHM 525 P LQ+ ARA + L G V + ERE L + Sbjct: 502 PGLQVVARAHSDAEIEHLEKHGAQRVIMGEREIARGMLEL 541 >gi|300312759|ref|YP_003776851.1| Kef-type K+ transport system, membrane component protein [Herbaspirillum seropedicae SmR1] Length = 587 Score = 106 bits (265), Expect = 5e-24, Method: Compositional matrix adjust. Identities = 107/382 (28%), Positives = 190/382 (49%), Gaps = 17/382 (4%) Query: 7 IALVYLLAAVAAVPIAKRLGLGAVLGYLLAGIVIGPWG--LGLITEVKHILEFSEFGVVL 64 +A++ L+A + V + +L VLGY++AG++IGP LI + K + SE GV+ Sbjct: 11 MAIIMLIAGIVTV-VFNKLKQPVVLGYIVAGVIIGPHTPPYDLIQDEKTVHILSELGVIF 69 Query: 65 LLFLIGLELDPKRLWALRRSIFGWGSIQVAAVSLALLAAALAAGVP--WRIGLIAALG-- 120 LLF +GLE K+L + + F I AA L ++ G+ W+ LG Sbjct: 70 LLFSLGLEFSLKKLAKVGATAF----IGAAAEILLMIWIGYEIGLYFGWKRMDAIFLGAM 125 Query: 121 MSLSSTAIALATLGERKLTATPAGSAARPILLFQDIAAIPMMAAVPLLGVAASQGSGQGW 180 +++SST I + L E + IL+ +DI AI M+A + + + S S + + Sbjct: 126 LAVSSTTIIVKALNELGMKNEKFAQIIFGILIVEDILAIGMIALLSGVATSGSVDSTEVF 185 Query: 181 LHAGQVALVIALLIFGGRVLMRPILRIIAKTDMREIFTAFALLLVIATCLLMQSVGMSMA 240 G++ L + + + G + + +L I++ E+ L ++ CLL+ + S+A Sbjct: 186 TTVGKLVLFMTVSLVVGILAVPRLLSFISRFKSNEMLLVAVLGVLFGFCLLVMKLQYSVA 245 Query: 241 LGTFLAGVLLADSEYRHALESDLEPFKGLLLGLFFIAVGMSVDFGVF--FAQPWRILGMV 298 LG FL G ++A+S + H +E +EP + + +FF+A+G+ D V + P ++ + Sbjct: 246 LGAFLVGAVMAESRHLHRIERLVEPIRDMFSAIFFVAIGLLFDPNVLVKYWMPITVITLA 305 Query: 299 LGFLAIKTCVMWALGRCFGIPRGQQ-LFFAFLLSQGGEFAFIVFGAAVTAKVLTPETSSL 357 + F + +C LG G+ + L+Q GEF+FI+ + KV + + Sbjct: 306 VVFGKLISC---GLGTFIAGQGGRTPMRVGMGLAQIGEFSFIIAALGQSLKVTSDFLYPI 362 Query: 358 LVVVVALSMLVTPLLLLVHDRL 379 +V V A++ L+TP L+ D L Sbjct: 363 VVAVSAVTALLTPYLIKAADPL 384 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.144 0.431 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,702,967 Number of extensions: 72808 Number of successful extensions: 422 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 415 Number of HSP's successfully gapped: 4 Length of query: 609 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 518 Effective length of database: 1,188,985 Effective search space: 615894230 Effective search space used: 615894230 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 133 (55.8 bits)