BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0063.p (241 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313671|ref|YP_003777763.1| OmpA family protein [Herbaspiri... 479 e-136 gi|300313316|ref|YP_003777408.1| lipoprotein [Herbaspirillum ser... 84 7e-18 gi|300312990|ref|YP_003777082.1| signal peptide protein [Herbasp... 84 9e-18 gi|300310091|ref|YP_003774183.1| outer membrane protein and rela... 80 2e-16 gi|300310619|ref|YP_003774711.1| OmpA protein [Herbaspirillum se... 56 3e-09 >gi|300313671|ref|YP_003777763.1| OmpA family protein [Herbaspirillum seropedicae SmR1] Length = 241 Score = 479 bits (1232), Expect = e-136, Method: Compositional matrix adjust. Identities = 240/241 (99%), Positives = 240/241 (99%) Query: 1 MKKLSIAAIAGVLALSGCADMSPTQRGTATGAGIGAGLGGIIGAAPGGGGGGRALGGAAI 60 MKKLSIAAIAGVLALSGCADMSPTQRGTATGAGIGAGLGGIIGAA GGGGGGRALGGAAI Sbjct: 1 MKKLSIAAIAGVLALSGCADMSPTQRGTATGAGIGAGLGGIIGAATGGGGGGRALGGAAI 60 Query: 61 GGAIGAVAGNIWSNRMENQKRAMEEATQGTGVQVSQTADNRLKLEIPSDISFDTGRADIK 120 GGAIGAVAGNIWSNRMENQKRAMEEATQGTGVQVSQTADNRLKLEIPSDISFDTGRADIK Sbjct: 61 GGAIGAVAGNIWSNRMENQKRAMEEATQGTGVQVSQTADNRLKLEIPSDISFDTGRADIK 120 Query: 121 PEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQRASRTRDYLSSRGVAAN 180 PEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQRASRTRDYLSSRGVAAN Sbjct: 121 PEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQRASRTRDYLSSRGVAAN 180 Query: 181 RFSIQGRGSHEPLVANTSDANRARNRRVEIFVAEQQAAAPQQAPAGYAPGNYPPPPPPQR 240 RFSIQGRGSHEPLVANTSDANRARNRRVEIFVAEQQAAAPQQAPAGYAPGNYPPPPPPQR Sbjct: 181 RFSIQGRGSHEPLVANTSDANRARNRRVEIFVAEQQAAAPQQAPAGYAPGNYPPPPPPQR 240 Query: 241 Y 241 Y Sbjct: 241 Y 241 >gi|300313316|ref|YP_003777408.1| lipoprotein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 84.3 bits (207), Expect = 7e-18, Method: Compositional matrix adjust. Identities = 58/170 (34%), Positives = 93/170 (54%), Gaps = 15/170 (8%) Query: 56 GGAAIGGAIGAVAGNIWSNRMENQKRAMEEATQ-------GTGVQVSQTA----DNRLKL 104 G I G +++G + S+ + Q+ A + AT G++VS + DN L Sbjct: 102 GQLKIDGTNVSISGEV-SHEAQRQQIASDMATSLNSSYIINNGLRVSTSEQGVLDNTLAN 160 Query: 105 EIPSDISFDTGRADIKPEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQR 164 + +SF++GRA + PE + +LD AAT+++ V I+G+TDN G A N LSL R Sbjct: 161 RV---VSFESGRATLTPEGKRILDEIAATMLQLKGRRVEIIGNTDNEGLRASNISLSLAR 217 Query: 165 ASRTRDYLSSRGVAANRFSIQGRGSHEPLVANTSDANRARNRRVEIFVAE 214 A + YLS++GV + + G+G P+ +N + RARNRR+E +A+ Sbjct: 218 ADAVKAYLSTKGVDDSLLTTSGQGPDRPVASNATSEGRARNRRIEFRIAK 267 >gi|300312990|ref|YP_003777082.1| signal peptide protein [Herbaspirillum seropedicae SmR1] Length = 203 Score = 84.3 bits (207), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 45/111 (40%), Positives = 62/111 (55%) Query: 103 KLEIPSDISFDTGRADIKPEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSL 162 K+ +D FD +A +KP + LD L + VGHTD+ GS A N LS+ Sbjct: 89 KVTYAADAFFDFDKATLKPAGKAKLDELVGNLKAINLEVIIAVGHTDSIGSVAYNQKLSV 148 Query: 163 QRASRTRDYLSSRGVAANRFSIQGRGSHEPLVANTSDANRARNRRVEIFVA 213 +RA + YL S+GV ANR +G+G +P+ N + A RA+NRRVEI V Sbjct: 149 RRAESVKAYLVSKGVEANRVYTEGKGKSQPVADNKTAAGRAKNRRVEIEVV 199 >gi|300310091|ref|YP_003774183.1| outer membrane protein and related peptidoglycan-associated (lipo)proteins [Herbaspirillum seropedicae SmR1] Length = 271 Score = 79.7 bits (195), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 41/105 (39%), Positives = 65/105 (61%) Query: 110 ISFDTGRADIKPEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQRASRTR 169 +SF++G+A + PE + +LD AAT+++ V I+G+TDN G A N LSL RA + Sbjct: 167 VSFESGQATLTPEGKRILDEIAATMLKLKGRHVEIIGNTDNEGLRASNISLSLARADAVK 226 Query: 170 DYLSSRGVAANRFSIQGRGSHEPLVANTSDANRARNRRVEIFVAE 214 YL ++GV + S G+G P+ +N + RA+NRR+E +A+ Sbjct: 227 AYLGAKGVDESLLSTSGQGPDRPVASNATAEGRAKNRRIEFRIAK 271 >gi|300310619|ref|YP_003774711.1| OmpA protein [Herbaspirillum seropedicae SmR1] Length = 190 Score = 55.8 bits (133), Expect = 3e-09, Method: Compositional matrix adjust. Identities = 34/104 (32%), Positives = 49/104 (47%) Query: 109 DISFDTGRADIKPEMRPVLDRFAATLVENPATTVTIVGHTDNTGSDAINDPLSLQRASRT 168 D+ F+ G + P + L + A + + PA + GH DN GS+ ND LSL+RA Sbjct: 86 DLLFELGTDRLTPASQDALKKAIAEIKDFPAGEFVLTGHADNIGSETNNDALSLRRARLI 145 Query: 169 RDYLSSRGVAANRFSIQGRGSHEPLVANTSDANRARNRRVEIFV 212 L V A + G+G+ +P V RNR VEI + Sbjct: 146 ERELRRADVKALSIEVIGKGARDPRVPQKRGVAEPRNRFVEIKI 189 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.313 0.131 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 493,093 Number of extensions: 16552 Number of successful extensions: 50 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 50 Number of HSP's successfully gapped: 5 Length of query: 241 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 158 Effective length of database: 1,226,865 Effective search space: 193844670 Effective search space used: 193844670 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 129 (54.3 bits)