BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0059.p (506 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313659|ref|YP_003777751.1| amidase family protein [Herbasp... 1016 0.0 gi|300309358|ref|YP_003773450.1| amidase family protein [Herbasp... 214 1e-56 gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer h... 158 1e-39 gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Her... 137 2e-33 gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotr... 117 2e-27 >gi|300313659|ref|YP_003777751.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 506 Score = 1016 bits (2628), Expect = 0.0, Method: Compositional matrix adjust. Identities = 501/506 (99%), Positives = 502/506 (99%) Query: 1 MQDKLVEKSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAA 60 MQDKLVEKSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAA Sbjct: 1 MQDKLVEKSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAA 60 Query: 61 EQAVIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIV 120 EQAVIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIV Sbjct: 61 EQAVIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIV 120 Query: 121 AGKTNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGS 180 AGKTNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGS Sbjct: 121 AGKTNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGS 180 Query: 181 LRIPAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDP 240 LRIPAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDP Sbjct: 181 LRIPAAKCGVVGFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDP 240 Query: 241 LGYAVSDSEFASLPPVDLSQLRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYVKLCEPVD 300 LGYAVSDSEFASLP VDLSQLRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYVKLCEPVD Sbjct: 241 LGYAVSDSEFASLPQVDLSQLRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYVKLCEPVD 300 Query: 301 VDMGEAHRAFDVIRAEAFVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWAQSEQT 360 VDMGEAHRAFDVIRAEAFVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWAQSEQT Sbjct: 301 VDMGEAHRAFDVIRAEAFVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWAQSEQT 360 Query: 361 RIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVALENYYRWLALTYVVTLATNP 420 RIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVALENYYRWLALTYVVTLATNP Sbjct: 361 RIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVALENYYRWLALTYVVTLATNP 420 Query: 421 AISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQAFSGNETLCRPRPDLAKLKTVH 480 AISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQAF+GNETL RPRPDL KLKTVH Sbjct: 421 AISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQAFAGNETLRRPRPDLTKLKTVH 480 Query: 481 APLTSIVTDPPLLQGQGMEQSGGPAV 506 APLTSIVTDPPLLQGQG EQSGGPAV Sbjct: 481 APLTSIVTDPPLLQGQGTEQSGGPAV 506 >gi|300309358|ref|YP_003773450.1| amidase family protein [Herbaspirillum seropedicae SmR1] Length = 482 Score = 214 bits (545), Expect = 1e-56, Method: Compositional matrix adjust. Identities = 167/463 (36%), Positives = 238/463 (51%), Gaps = 11/463 (2%) Query: 4 KLVEKSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATC-FERARGEARAAEQ 62 +LV A+ L I SK++S E+++A + IE +NPH+NA+ + E EA+ A+Q Sbjct: 3 QLVTLDALALSAAIRSKEVSCREVMQAYLEHIEKMNPHVNAIVSLRPPEELLDEAKRADQ 62 Query: 63 AVIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAG 122 + G+ LG +HG+P KDL +GL T+ GSPL D VAR+R AGAI G Sbjct: 63 LLAQGRYLGWMHGMPQAPKDLASCKGLPTSKGSPLSSMAPDQSDATCVARVRDAGAIFIG 122 Query: 123 KTNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLR 182 KTNV E G G+NS N ++G T N ++ AGGSSGG+ AALA +LP+ GSD GSLR Sbjct: 123 KTNVSEFGLGSNSYNPLFGVTRNAYDFSRIAGGSSGGAGAALAMRMLPVADGSDMMGSLR 182 Query: 183 IPAAKCGVVGFRPSPGLVP-SERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDPL 241 PAA V GFRP+ G VP L +S GPMGR VAD G PL Sbjct: 183 NPAAFNNVYGFRPTQGRVPYGSGGELFLQQLSTEGPMGRTVADVARLFATQAGYDPQCPL 242 Query: 242 GYAVSDSEFASLPPVDLSQLRIGYTEDF-GVCDVDNGIR---QVFRDKMAAIAPYVKLCE 297 + A+ D RIG+ ++ G ++ + + A I V+ C Sbjct: 243 SSDTDVTGDAAQLKRDFKGARIGWLGNYDGYLPIEEEVMVLCEAALSDFADIGCTVEQCR 302 Query: 298 PVDVDMGEAHRAFDVIRAEAFVAGFDAAYRRDPASLG---PNTRANYEMGAAMSLLDCAW 354 P D M + + V R + V G + Y DPA G P + E G ++ D Sbjct: 303 P-DYPMEKLWETWLVHR-QWLVGGNLSVYFADPARRGLLKPEAQWEVESGQYLTAADVFR 360 Query: 355 AQSEQTRIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVALENYYRWLALTYVV 414 A ++ +R +L+E++D +L P+ + PF + + + V G ++ Y+RW+ + Sbjct: 361 ASMARSDWYRAIHKLFEQFDYLLLPSAQMFPFDVATKWPRNVAGREMDTYHRWMEVVIGP 420 Query: 415 TLATNPAISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALE 457 TLA PA+S+P G G+P G+Q+ GP R D VL A ALE Sbjct: 421 TLAGLPAMSVPVGFGANGLPMGMQIIGPSRDDRGVLQLAYALE 463 >gi|300312309|ref|YP_003776401.1| 6-aminohexanoate-cyclic-dimer hydrolase [Herbaspirillum seropedicae SmR1] Length = 482 Score = 158 bits (399), Expect = 1e-39, Method: Compositional matrix adjust. Identities = 143/466 (30%), Positives = 208/466 (44%), Gaps = 23/466 (4%) Query: 8 KSAVELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAAEQAVIDG 67 + ++L Q IG +LSP E L I R+E +NP +NAV + EQ+V G Sbjct: 13 QDGLDLAQRIGQGELSPGEALAQAIERLEQVNPALNAVAEKLYH-------LGEQSVAQG 65 Query: 68 KPLGLLHGLPIGIKDL-EETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAGKTNV 126 P G G+P+ KDL G + GS L + N+ D LV RL+ AG + G T Sbjct: 66 LPDGPFRGVPLLTKDLFTPLAGARMSNGSLLLKDNVMPVDAELVTRLKKAGFTIFGTTTS 125 Query: 127 PEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLRIPAA 186 PE G + + ++GAT NP++ + GGSSG +AA +A +LP+ G+D GGSLR+PA+ Sbjct: 126 PEFGTSYTTESRLFGATRNPWSLDHSCGGSSGAAAALVAARVLPIAHGNDGGGSLRVPAS 185 Query: 187 KCGVVGFRPSPGLVP-SERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDPLGYAV 245 CGV G +PS GL+P GW +S M V D+ L G P Sbjct: 186 ACGVFGLKPSRGLMPMGPLAGEGWAGMSTSHVMSLSVRDSAAVLDQLAGADLGAPYAAPH 245 Query: 246 SDSEFASLPPVDLSQ-LRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYV-----KLCEPV 299 F + Q LRIG + + + AA+ + + C PV Sbjct: 246 YRQSFLAAATGAAPQGLRIGLVTHLPPWPTHPDCSEAVQ-RTAALCQQLGHHVEETCLPV 304 Query: 300 DVDMGEAHRAFDVIRAEA----FVAGFDAAYRRDPASLGPNTRANYEMGAAMSLLDCAWA 355 D + F +I ++ + A D +L R +S D A A Sbjct: 305 D-GLEFYDTVFTIIGSQTRNLMNLLARMAGQPLDEQALEARHRVILRDKGQLSGADYAAA 363 Query: 356 QSEQTRIFRRFQQLYERYDLILSPTTPVSPFPWSELYLKEVNGVA--LENYYRWLALTYV 413 RR +L +RYDLIL+PT P L ++E VA + ++ + T + Sbjct: 364 VDYLHAFGRRMAELMQRYDLILTPTMAQPPARIGALTVREEQSVAELIHTFHGFSPFTAL 423 Query: 414 VTLATNPAISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAALEQA 459 + PA+S+P + +G+P G F D +LA AA LEQA Sbjct: 424 CNASGQPAMSVPLHWNDQGLPIGSHFCAGFGDDTLLLAVAAQLEQA 469 >gi|300311728|ref|YP_003775820.1| indole acetamide hydrolase [Herbaspirillum seropedicae SmR1] Length = 468 Score = 137 bits (344), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 154/484 (31%), Positives = 231/484 (47%), Gaps = 57/484 (11%) Query: 5 LVEKSAVELRQLIGSKQLSPVELLEACIARIE---DINPHINAVTATCFERARGEARAAE 61 LV+ + +E+RQ + ++ S +E ++A IA E D+N ++ E+ R +AR + Sbjct: 3 LVDMTLIEVRQGLSDRRFSCLEYVDALIAETEAQWDLNCYLQRDP----EQLRSQARMLD 58 Query: 62 QAVIDGKPLGLLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVA 121 + G L G+PI IKD + G+ +T G+ R N+P R +V L AGA+ A Sbjct: 59 EQGGHGP----LSGIPIAIKDNIDVAGIASTAGTAALRNNVPQRHATVVEYLFGAGALHA 114 Query: 122 GKTNVPEMGAGANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSL 181 GK N+ E+ G + N V+G NP++ L AGGSSGG A LA L P G+DTGGS+ Sbjct: 115 GKANMHELAFGITNNNGVFGPCRNPYDAELIAGGSSGGCGALLAARLAPAAVGTDTGGSV 174 Query: 182 RIPAAKCGVVGFRPSPGLVPSERKLLGWTPISVV----GPMGRDVADTVLQLRATLGLHA 237 RIPAA CGVVG RP+ G PIS GPM R VAD L + G Sbjct: 175 RIPAALCGVVGLRPTSRRYSQH----GVVPISHTRDTPGPMARSVADVALLDQIMAGSRD 230 Query: 238 SDPLGYAVSDSEFASLPPVDLSQLRIGYTEDFGVCDVDNGIRQVFRDKMAAIAPYVKL-C 296 S PL +LPP SQLR+G + ++ +V + AA+ + Sbjct: 231 SAPL---------LALPP---SQLRLGVLRNPSWQGLEE---EVEANANAALMQLREAGV 275 Query: 297 EPVDVDMGE----AHRA-FDVIRAE------AFVAGFDA--AYRRDPASLG-PNTRANYE 342 E VDVD+ +H A F ++ E A++ DA +R A +G P+ + Sbjct: 276 ELVDVDLPTLAPLSHAAGFAIVLHEFLIDLPAYLQASDARDVIKRVLAEVGSPDVARIIQ 335 Query: 343 MGAAMSLLDCAWAQSEQTRIFRRFQQLY------ERYDLILSPTTPVSPFPWSELYLKEV 396 + + A+ + + R R Q LY + D ++ PTT ++ P E + Sbjct: 336 ATLDQPVPEHAYRLALEQR--RTMQALYRDCFRSQGVDALIFPTTALTARPLGEDDTVAL 393 Query: 397 NGVALENYYRWLALTYVVTLATNPAISLPCGRDHRGMPFGLQVTGPFRGDARVLACAAAL 456 G + + + ++A P+IS+P G G+P GL++ GP D R+L AA+L Sbjct: 394 RGERVPTFPAFARNADGASIAGLPSISIPVGLSEYGLPVGLELDGPEGSDRRLLQVAASL 453 Query: 457 EQAF 460 E Sbjct: 454 ETVL 457 >gi|300313942|ref|YP_003778034.1| Asp-tRNAAsn/Glu-tRNAGln amidotransferase subunit A [Herbaspirillum seropedicae SmR1] Length = 488 Score = 117 bits (294), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 135/481 (28%), Positives = 213/481 (44%), Gaps = 42/481 (8%) Query: 12 ELRQLIGSKQLSPVELLEACIARIEDINPHINAVTATCFERARGEARAAEQAVIDGKPLG 71 EL + ++++S EL + RI+ +NA E +A+ A+ + D G Sbjct: 8 ELSAQLQARKISATELATLFLDRID--ASDLNAFLHVDRELTLAQAKVADSRLSDNNA-G 64 Query: 72 LLHGLPIGIKDLEETEGLLTTYGSPLYRGNIPARDNALVARLRAAGAIVAGKTNVPEMGA 131 L G+PI KD+ T G TT GS + + D +V + AAG + GK N E Sbjct: 65 QLTGVPIAHKDIFVTRGWRTTAGSRMLDNYVSPFDATVVEQFNAAGMVTLGKLNCDEFAM 124 Query: 132 GANSRNAVWGATGNPFNPLLNAGGSSGGSAAALATDLLPLCSGSDTGGSLRIPAAKCGVV 191 G+ + N+ +G T NP++ GGSSGGSAAA+A L P + +DTGGS+R PA+ CGV Sbjct: 125 GSGNENSYFGPTLNPWDKRAVPGGSSGGSAAAVAARLAPAATATDTGGSIRQPASLCGVT 184 Query: 192 GFRPSPGLVPSERKLLGWTPISVVGPMGRDVADTVLQLRATLGLHASDPLGYAVSDSEFA 251 G +P+ G V + + + GP+ + D L L A G D +F Sbjct: 185 GIKPTYGSVSRYGMIAFASSLDQGGPIAKTAEDCGLLLNAMTGFDERDSTSLQRPKEDFT 244 Query: 252 SLPPVDLSQLRIGYTEDFG----VCDVDNGIRQVFRD------KMAAIA-PYVKLCEPVD 300 L LRIG ++F DV+ +R + ++ I+ P +L PV Sbjct: 245 RSLNDSLQGLRIGVPKEFFGAGLAADVEAAVRAALSEFEKLGAQLVEISLPKTELSIPVY 304 Query: 301 VDMGEAHRAFDVIRAEAFVAGFDAA--------YRRDPAS-LGPNTRANYEMGA---AMS 348 + A + ++ R + G AA YR+ A G + +G+ + Sbjct: 305 YVIAPAEASSNLSRFDGVRYGHRAADYGNLDDMYRKSRAEGFGEEVKRRILVGSYVLSHG 364 Query: 349 LLDCAWAQSEQTR--IFRRFQQLY----ERYDLILSPTTPVSPFPWSELYLKEVNGVALE 402 D + Q+++ R I + FQ + D+I+ P +P W L + + Sbjct: 365 YYDAYYLQAQKIRRLIAQDFQNALAGANRQCDVIMGPVSPT--VAWD---LGDKTNDPVA 419 Query: 403 NYYRWLALTYVVTLATNPAISLPC----GRDHRGMPFGLQVTGPFRGDARVLACAAALEQ 458 NY + T +LA P +S+PC G + P GLQ+ G + +AR+L A +Q Sbjct: 420 NYLADI-FTLSTSLAGLPGMSIPCGFGQGEKNGKRPVGLQIIGNYFDEARLLNVAHQFQQ 478 Query: 459 A 459 A Sbjct: 479 A 479 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.410 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,567,855 Number of extensions: 65816 Number of successful extensions: 216 Number of sequences better than 1.0e-08: 11 Number of HSP's gapped: 203 Number of HSP's successfully gapped: 12 Length of query: 506 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 417 Effective length of database: 1,198,455 Effective search space: 499755735 Effective search space used: 499755735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)