BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0058.p (404 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313658|ref|YP_003777750.1| transport transmembrane protein... 805 0.0 gi|300309906|ref|YP_003773998.1| major facilitator superfamily p... 277 1e-75 gi|300312451|ref|YP_003776543.1| D-galactonate transporter prote... 152 6e-38 gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspir... 131 1e-31 gi|300309918|ref|YP_003774010.1| major facilitator superfamily p... 113 3e-26 >gi|300313658|ref|YP_003777750.1| transport transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 404 Score = 805 bits (2080), Expect = 0.0, Method: Compositional matrix adjust. Identities = 404/404 (100%), Positives = 404/404 (100%) Query: 1 MDAAVRKGKYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLG 60 MDAAVRKGKYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLG Sbjct: 1 MDAAVRKGKYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLG 60 Query: 61 YSLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPA 120 YSLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPA Sbjct: 61 YSLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPA 120 Query: 121 ASVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVF 180 ASVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVF Sbjct: 121 ASVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVF 180 Query: 181 ALVYWFCVRPVRPGVGGAGAINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYL 240 ALVYWFCVRPVRPGVGGAGAINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYL Sbjct: 181 ALVYWFCVRPVRPGVGGAGAINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYL 240 Query: 241 MTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMY 300 MTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMY Sbjct: 241 MTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMY 300 Query: 301 SAASVEGVIAWQCLAYFFKSFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLV 360 SAASVEGVIAWQCLAYFFKSFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLV Sbjct: 301 SAASVEGVIAWQCLAYFFKSFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLV 360 Query: 361 IGFLVSAFANYDYAFSFLIGAAFFSVLVSLFIRTHKSDSQMQPA 404 IGFLVSAFANYDYAFSFLIGAAFFSVLVSLFIRTHKSDSQMQPA Sbjct: 361 IGFLVSAFANYDYAFSFLIGAAFFSVLVSLFIRTHKSDSQMQPA 404 >gi|300309906|ref|YP_003773998.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 414 Score = 277 bits (708), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 150/385 (38%), Positives = 236/385 (61%), Gaps = 2/385 (0%) Query: 11 KYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGW 70 K + L L+L W V Y DR ++ A I+ EF L G++LS+F++ Y+L+QL GGW Sbjct: 10 KMICLSALFLAWAVGYADRIVMSTAIIPISKEFALDAQQAGMVLSAFYVSYALMQLMGGW 69 Query: 71 LADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKGVAENF 130 L+DRYGSR V+V+ ++ WSLFTG+T MAWS SL++IRF+FG GEG F AS VAE F Sbjct: 70 LSDRYGSRIVVVMCVVAWSLFTGLTSMAWSFASLLLIRFLFGAGEGCFSPASSVTVAEVF 129 Query: 131 SRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYWFCVRP 190 + ER + S L+S+ ++G+ + ++A +++ GWR FH + G++ A + W +R Sbjct: 130 PQQERARAKSFLISTVFLGNAVGSGMVALTVVYLGWRGTFHILAFVGMIVAAILWLALRS 189 Query: 191 -VRPGVGGAGAINKQAFMALMKMPLMWQIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLK 249 + + A K L+K P ++ A+WF SI+ G+ +WMP++L+ V ++L Sbjct: 190 GMAAQQARSAARPKNQLRELVKNPTALRLTAIWFCTSIVYIGMISWMPSFLLKVYQIDLL 249 Query: 250 AVGLLLPLPYIMAGLSTAIGGWVMVRFFDGREKLLLIGSSLLTAVFVYCMYSAASVEGVI 309 +G+ +PY++A L T + GW++ + GREK ++G +L AVF+ M S +E ++ Sbjct: 250 HIGIASAIPYLLAFLGTNVVGWLLDKHGKGREKYFMMGGALACAVFLALMISTTVIELLV 309 Query: 310 AWQCLAYFFKSFVLATCIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFA 369 + L +FV AT ++P K + L+GS G++N GGQLAG I+P+V+G L+ AF Sbjct: 310 VYWTLCLLSFNFVYATVFSVPLKHFPSHLIGSATGLMNFGGQLAGSIAPVVMGSLIVAFN 369 Query: 370 N-YDYAFSFLIGAAFFSVLVSLFIR 393 Y AF FL+G+A S L+++ R Sbjct: 370 GAYLAAFWFLVGSAMMSFLIAMTWR 394 >gi|300312451|ref|YP_003776543.1| D-galactonate transporter protein [Herbaspirillum seropedicae SmR1] Length = 439 Score = 152 bits (383), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 126/438 (28%), Positives = 210/438 (47%), Gaps = 54/438 (12%) Query: 11 KYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQLPGGW 70 +YL+L LL++ V+Y DRA ++ A + + +E L P +G + S+F Y L QLPGGW Sbjct: 12 RYLILFLLFVVTTVNYADRATLSIAGSAMKTELSLDPVMMGYIFSAFSWAYVLAQLPGGW 71 Query: 71 LADRYGSRPVIVISILLWSLFT----GVTGM--AWSVTSLVMIRFIFGVGEG-AFPAASV 123 L DR+GS+ + + SI LWS+FT GVT M A +V SL ++RF+ G+ E +FPA Sbjct: 72 LLDRFGSKKIYMWSIALWSVFTFLQAGVTWMSTAAAVASLFLLRFMVGLAEAPSFPANG- 130 Query: 124 KGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALV 183 + VA F ER S++ S+ Y ++L L+A ++ Y W VF +G+ G+ AL+ Sbjct: 131 RIVAAWFPTKERGTASAIFNSAQYFAAVLFTPLMAWIVHTYSWHHVFTVMGVVGIALALI 190 Query: 184 YWFCV-----------------------------RPVRPGVGGAGAINKQAFMALMKMPL 214 + + + P G G I + L+ + Sbjct: 191 WGRIIYSPKDHPRINQAELAHIESGGGLVDMDNRKVAAPSTKGRGYIRQ-----LLSNRM 245 Query: 215 MWQIVAVWFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWVMV 274 + + + +++I TW P YL+ RG+++ G + +P + + +GG + Sbjct: 246 LLGVYIGQYCINVITYFFLTWFPVYLVQERGMSILKAGFVASIPAVCGFIGGVVGGLISD 305 Query: 275 RFFDG------REKLLLIGSSLLTAVFVYCMYSAAS--VEGVIAWQCLAYFFKSFVLATC 326 R K+ ++G LL+ + C Y A V G++A LA+F K V A Sbjct: 306 RLIKRGLSVTWARKIPIVGGMLLSMTMILCNYVEAQWMVVGIMA---LAFFGKG-VGALG 361 Query: 327 IALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAAFFSV 386 A+ Q+VG G+ N+ G +AG +P+VIG++V ++ A F+ A +V Sbjct: 362 WAVVADTAPKQIVGLSGGLFNMFGNVAGITTPIVIGYIVKETGSFSGALVFVGINALVTV 421 Query: 387 LVSLFIRTHKSDSQMQPA 404 + L I +++ A Sbjct: 422 ISYLVIVKEIKRVELKDA 439 >gi|300313744|ref|YP_003777836.1| D-glucarate permease [Herbaspirillum seropedicae SmR1] Length = 441 Score = 131 bits (329), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 108/428 (25%), Positives = 201/428 (46%), Gaps = 42/428 (9%) Query: 6 RKGKYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGYSLLQ 65 +K ++++V L+++ + V+ DRA + A + EF + ++ G L S F YSL Q Sbjct: 11 KKTHFRWVVGALIFIVYTVAAADRANLGVALPFLRKEFVMSNTEAGALASIFLFAYSLAQ 70 Query: 66 LPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAASVKG 125 LP G R+G V +++L S+FTG+ G A S+ +L + RF G+ EG P Sbjct: 71 LPSGLAYTRFGIPRVFSLAMLATSVFTGLIGTASSLLALKIFRFGLGLAEGPLPIGITST 130 Query: 126 VAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFALVYW 185 + F E+ M+ + +++ G +L P + A ++ ++GWR +F++ + GV+ ++V W Sbjct: 131 INNWFPAREKGTMTGIFLAAAKFGPVLVPPVCAVIMYYWGWRHIFYFFAVPGVILSVV-W 189 Query: 186 FCVRPVRPGVG---------------GAGA-----------------INKQAFMALMKMP 213 V RP GA A I +++ +A ++ Sbjct: 190 LMVVKNRPSESPHVSAEELEHINDSQGAAAGQDADPAVTRDFALLDRIIRRSKVATIETT 249 Query: 214 ----LMWQIVAV---WFGLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLST 266 W I+ +F ++ I L W+PTYL++V+G ++ +G++ P++ A + Sbjct: 250 KGVFCSWNIMGAALGYFFMTAIVNVLLAWIPTYLISVKGYSIANMGMVAAAPWVGAVIGN 309 Query: 267 AIGGWVMVRFFDGREKLLLIGSSLLTAV-FVYCMYSAASVEGVIAWQCLAYFFKSFVLAT 325 +GG + R+ + R K ++ S+ T + V + S A A L + +T Sbjct: 310 LLGGAMTDRWLEKRRKPAMMMSAFFTTIMMVILINSPADPLLYGALLLLTGILLNIGYST 369 Query: 326 CIALPTKMLQAQLVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAAFFS 385 + P M ++ MVN GQL G +PL GFL+ ++ ++++ + F+ + Sbjct: 370 YMVYPMGMTSKKVYPMACAMVNTLGQLGGACAPLAAGFLLDSY-SWNHVWIFMAVCSILC 428 Query: 386 VLVSLFIR 393 + L IR Sbjct: 429 FITILTIR 436 >gi|300309918|ref|YP_003774010.1| major facilitator superfamily protein [Herbaspirillum seropedicae SmR1] Length = 428 Score = 113 bits (283), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 107/423 (25%), Positives = 191/423 (45%), Gaps = 25/423 (5%) Query: 2 DAAVRKGKYKYLVLVLLYLGWCVSYIDRAAITFAATQIASEFHLKPSDLGILLSSFFLGY 61 + + R + + + L L L V+Y+DR+ ++ A ++ E +L S +G+LLS+F L Y Sbjct: 6 EKSPRLKRIQVVALSFLMLAGIVNYLDRSTLSIANHSVSQELNLSASQMGLLLSAFSLAY 65 Query: 62 SLLQLPGGWLADRYGSRPVIVISILLWSLFTGVTGMAWSVTSLVMIRFIFGVGEGAFPAA 121 + QLP G + D++G+R ++ + +WS+ G ++ ++ R G+GE A Sbjct: 66 AFSQLPIGAMLDKFGARVMLGAGMFIWSVAQLAGGFVNNLQQFLVARVFLGLGEAPQFPA 125 Query: 122 SVKGVAENFSRDERPKMSSLLMSSNYIGSMLAPLLIAPLILHYGWRAVFHYIGIAGVVFA 181 K V+E F+ ER + + ++S+ IG LAP ++ L+L +GWR +F +G+ G+ + Sbjct: 126 GAKVVSEWFAMRERGGPTGIFVASSTIGPALAPPILTILMLSFGWRMMFVIMGVLGIAVS 185 Query: 182 LVYWFCVRPVRPGV------------------GGAGAINKQAFMALMKMPLMWQIVAVWF 223 V W+ R V ++ + +L W ++ + Sbjct: 186 -VGWYLFYRNRNEVQLTPSEVLYLADDHDNDPNANAKLSWSEWKSLFTYRTTWGMLFGFM 244 Query: 224 GLSIINKGLDTWMPTYLMTVRGLNLKAVGLLLPLPYIMAGLSTAIGGWV----MVRFFDG 279 G+ + TW+P YL R L++ G ++ +PYI L G++ M R Sbjct: 245 GVIYMVWLYLTWLPAYLEHERHLSIARTGWVVVIPYIFGTLGMLSSGYIADGLMKRGMPA 304 Query: 280 -REKLLLIGSSLLTA-VFVYCMYSAASVEGVIAWQCLAYFFKSFVLATCIALPTKMLQAQ 337 R + I + L+ A F S I + LA F + AL + + + Sbjct: 305 IRSRKWPICAGLIGAGAFTIPAAYTPSTTMAIVYISLAMAFVNMASGGAWALVSVAIPRR 364 Query: 338 LVGSGVGMVNLGGQLAGFISPLVIGFLVSAFANYDYAFSFLIGAAFFSVLVSLFIRTHKS 397 LV S M N GG L G ++P++ G +V ++ A AF + LV +F+ Sbjct: 365 LVASLGSMQNFGGYLGGSLAPVITGVVVDQTHSFVNALLISAAVAFAAALVYMFVVDKPI 424 Query: 398 DSQ 400 +S Sbjct: 425 ESH 427 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.142 0.447 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,059,825 Number of extensions: 40291 Number of successful extensions: 335 Number of sequences better than 1.0e-08: 32 Number of HSP's gapped: 307 Number of HSP's successfully gapped: 36 Length of query: 404 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 317 Effective length of database: 1,207,925 Effective search space: 382912225 Effective search space used: 382912225 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)