BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0029.p (189 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313632|ref|YP_003777724.1| oxidoreductase [Herbaspirillum ... 384 e-108 gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein... 97 7e-22 gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogena... 96 1e-21 gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herb... 86 2e-18 gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herb... 82 3e-17 >gi|300313632|ref|YP_003777724.1| oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 260 Score = 384 bits (987), Expect = e-108, Method: Compositional matrix adjust. Identities = 189/189 (100%), Positives = 189/189 (100%) Query: 1 LKDWGGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSG 60 LKDWGGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSG Sbjct: 72 LKDWGGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSG 131 Query: 61 VIIHVSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASV 120 VIIHVSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASV Sbjct: 132 VIIHVSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASV 191 Query: 121 AFTQRTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYR 180 AFTQRTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYR Sbjct: 192 AFTQRTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYR 251 Query: 181 IDGGTVPTV 189 IDGGTVPTV Sbjct: 252 IDGGTVPTV 260 >gi|300311249|ref|YP_003775341.1| 3-oxoacyl-[acyl-carrier-Protein] reductase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 251 Score = 97.4 bits (241), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 63/183 (34%), Positives = 99/183 (54%), Gaps = 17/183 (9%) Query: 2 KDWGGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSGV 61 KD+G + IL+N G ++ + DE W + NL R+ RA+L M+ +G Sbjct: 82 KDFGSLSILVNNAGITQDQLA--MRMKDEEWDSVIATNLTAVGRLSRAVLRGMMKARTGR 139 Query: 62 IIHVSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASVA 121 II+++S+ P YAAAKA + S++LARE+ S+ + V VAPG+I+T+ + A Sbjct: 140 IINITSVVGEAGNPGQMN-YAAAKAGVAGMSRALAREIGSRNITVNCVAPGFIDTDMTRA 198 Query: 122 FTQRTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYRI 181 ++ +A L IP+GR +E+A +AFLASP++A ITGT + Sbjct: 199 LNEQQSAA--------------ILQQIPLGRLGAAEEIAYAVAFLASPQAAYITGTTLNV 244 Query: 182 DGG 184 +GG Sbjct: 245 NGG 247 >gi|300313172|ref|YP_003777264.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 254 Score = 96.3 bits (238), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 60/180 (33%), Positives = 95/180 (52%), Gaps = 5/180 (2%) Query: 5 GGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSGVIIH 64 G +D+L N G G D+ W FNLN RA+LP MLA+ +G I++ Sbjct: 77 GTIDVLFNCAG--YVAAGNILECDDKAWDFSFNLNAKAMFHTIRAVLPGMLAKKAGSIVN 134 Query: 65 VSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASVAFTQ 124 ++S + + AY A+KAA+ +KS+A + ++G+R ++ PG IE S + Q Sbjct: 135 IASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVAQGIRCNAICPGTIE---SPSLNQ 191 Query: 125 RTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYRIDGG 184 R + A + +++ ++G P+GR K +EVA L +LAS S TG+ + IDGG Sbjct: 192 RISTQAKETGKSEEEVRAAFVGRQPMGRIGKAEEVAALALYLASDESNFTTGSIHMIDGG 251 >gi|300312302|ref|YP_003776394.1| acetoacetyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 246 Score = 85.5 bits (210), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 55/180 (30%), Positives = 95/180 (52%), Gaps = 17/180 (9%) Query: 5 GGVDILINALGGSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQGSGVIIH 64 G VD+L+N G T G F +S + W A + NL + + ++ M +G G II+ Sbjct: 80 GEVDVLVNNAG--ITRDGQFRKMSKDDWDAVIDTNLNSLFNVTKQVIEGMCERGFGRIIN 137 Query: 65 VSSIQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETEASVAFTQ 124 +SS+ T Y+ AKA + ++ +LA+EV +KGV V +V+PG+I T+ A Sbjct: 138 ISSVNG-QKGQFGQTNYSTAKAGIHGFTMALAQEVATKGVTVNTVSPGYIGTDMVRAIR- 195 Query: 125 RTAAHAGTDYEGGKKIIMDWLGGIPVGRPAKPQEVADLIAFLASPRSASITGTEYRIDGG 184 +++ + GIPV R +P+E+A ++A++AS TG+++ ++GG Sbjct: 196 -------------PEVLEKIVSGIPVKRLGEPEEIASIVAWIASSEGGYATGSDFSLNGG 242 >gi|300311900|ref|YP_003775992.1| short chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 82.0 bits (201), Expect = 3e-17, Method: Compositional matrix adjust. Identities = 63/189 (33%), Positives = 91/189 (48%), Gaps = 25/189 (13%) Query: 1 LKDWGGVDILINALG--GSKTPGGGFAAISDEHWFAEFNLNLMPAVRMDRALLPNMLAQG 58 L ++GG+DI IN G G+ P A IS + W + NL A R +P MLA+G Sbjct: 78 LDEFGGLDIAINNAGAVGACKP---LAEISPQQWQDTLHANLTSAFLGARCQIPAMLARG 134 Query: 59 SGVIIHVSS-IQRVLPLPESTTAYAAAKAALTTYSKSLAREVTSKGVRVLSVAPGWIETE 117 +G I+ SS + LP AY AAKA L K +A + +G+R ++ PG ++T+ Sbjct: 135 AGAIVFTSSFVGSSAGLP-GMAAYGAAKAGLMGLVKGIAADYAVQGIRANALLPGGVDTD 193 Query: 118 ASVAFTQRTAAHAGTDYEGGKKIIMDWLGGI-PVGRPAKPQEVADLIAFLASPRSASITG 176 GG W G+ + R A+P+E+A FLASP ++ +TG Sbjct: 194 -----------------MGGDPQQKQWAAGLHAMKRIAQPEEIAAAALFLASPMASFVTG 236 Query: 177 TEYRIDGGT 185 T DGG Sbjct: 237 TALYADGGN 245 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 601,325 Number of extensions: 22907 Number of successful extensions: 186 Number of sequences better than 1.0e-08: 35 Number of HSP's gapped: 156 Number of HSP's successfully gapped: 35 Length of query: 189 Length of database: 1,619,870 Length adjustment: 80 Effective length of query: 109 Effective length of database: 1,241,070 Effective search space: 135276630 Effective search space used: 135276630 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)