BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0015.p (701 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313617|ref|YP_003777709.1| translation elongation factor [... 1434 0.0 gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspir... 1123 0.0 gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 ... 175 1e-44 gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 97 3e-21 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 86 1e-17 >gi|300313617|ref|YP_003777709.1| translation elongation factor [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1434 bits (3713), Expect = 0.0, Method: Compositional matrix adjust. Identities = 701/701 (100%), Positives = 701/701 (100%) Query: 1 MSRKTRIENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERG 60 MSRKTRIENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERG Sbjct: 1 MSRKTRIENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERG 60 Query: 61 ITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQ 120 ITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQ Sbjct: 61 ITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQ 120 Query: 121 PQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFS 180 PQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFS Sbjct: 121 PQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFS 180 Query: 181 GVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEANEELLEKYLSG 240 GVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEANEELLEKYLSG Sbjct: 181 GVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEANEELLEKYLSG 240 Query: 241 NPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPVDVPAIAGHGE 300 NPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPVDVPAIAGHGE Sbjct: 241 NPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPVDVPAIAGHGE 300 Query: 301 DDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNPVKGKKERLGR 360 DDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNPVKGKKERLGR Sbjct: 301 DDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNPVKGKKERLGR 360 Query: 361 ILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFPEPVISQAVEP 420 ILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFPEPVISQAVEP Sbjct: 361 ILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFPEPVISQAVEP 420 Query: 421 KTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDRMKREFNVEAT 480 KTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDRMKREFNVEAT Sbjct: 421 KTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDRMKREFNVEAT 480 Query: 481 VGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYEFVDAIKGGVV 540 VGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYEFVDAIKGGVV Sbjct: 481 VGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYEFVDAIKGGVV 540 Query: 541 PREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRMAGSMAFKEAM 600 PREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRMAGSMAFKEAM Sbjct: 541 PREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRMAGSMAFKEAM 600 Query: 601 KRAGPMLLEPMMQVEVETPEEFMGNVMGDLSSRRGMVQGMEDMVGGGKLVRAEVPLSEMF 660 KRAGPMLLEPMMQVEVETPEEFMGNVMGDLSSRRGMVQGMEDMVGGGKLVRAEVPLSEMF Sbjct: 601 KRAGPMLLEPMMQVEVETPEEFMGNVMGDLSSRRGMVQGMEDMVGGGKLVRAEVPLSEMF 660 Query: 661 GYSTTLRSLSQGRATYSMEFKHYAEAPRQVVEQLSGGKGKG 701 GYSTTLRSLSQGRATYSMEFKHYAEAPRQVVEQLSGGKGKG Sbjct: 661 GYSTTLRSLSQGRATYSMEFKHYAEAPRQVVEQLSGGKGKG 701 >gi|300309453|ref|YP_003773545.1| elongation factor G1 [Herbaspirillum seropedicae SmR1] Length = 701 Score = 1124 bits (2906), Expect = 0.0, Method: Compositional matrix adjust. Identities = 530/701 (75%), Positives = 609/701 (86%), Gaps = 1/701 (0%) Query: 1 MSRKTRIENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERG 60 M+RKT IE YRNIGISAHIDAGKTTTTER+LFYTGVNHKIGEVH+GAATMDWMEQEQERG Sbjct: 1 MARKTPIERYRNIGISAHIDAGKTTTTERVLFYTGVNHKIGEVHDGAATMDWMEQEQERG 60 Query: 61 ITITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQ 120 ITITSAATT +WKGMA N+P H INIIDTPGHVDFTIEVERSMRVLDGA MVY +VGGVQ Sbjct: 61 ITITSAATTCYWKGMANNFPAHHINIIDTPGHVDFTIEVERSMRVLDGACMVYCAVGGVQ 120 Query: 121 PQSETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFS 180 PQSETVWRQANKYKVPR+AFVNKMDR GA+FF+V +Q+ RLK +P+Q+P+GAE++F Sbjct: 121 PQSETVWRQANKYKVPRLAFVNKMDRTGANFFKVYEQMRARLKANPIPMQVPIGAEENFE 180 Query: 181 GVIDLVKMKAIIWDEASQGVLFKYEDIPLELEDTARHWRDLMVEQAAEANEELLEKYLSG 240 GVIDLVKM+AI WD+ASQG+ F Y DIP L+ A+ WR+ +VE AAEA+E+L+ KYL Sbjct: 181 GVIDLVKMRAIYWDDASQGMKFDYRDIPEHLKADAQKWRENLVETAAEASEDLMNKYLEE 240 Query: 241 NPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPVDVPAIAGHGE 300 LTE +IK +R RT++ EIVPM+ G+AFKNKGVQAMLD V++YLPSPVD+P + G E Sbjct: 241 GDLTEAEIKGAIRQRTISGEIVPMMCGTAFKNKGVQAMLDGVVEYLPSPVDIPPVPGLNE 300 Query: 301 DDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFFRVYSGIVNSGDTVYNPVKGKKERLGR 360 DD + R D+E FSALAFKI TDPFVGQL F R YSG +NSGDTV N VK KKER+GR Sbjct: 301 DDEPVVREAKDDEKFSALAFKIATDPFVGQLCFIRCYSGTLNSGDTVLNSVKSKKERIGR 360 Query: 361 ILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERMIFPEPVISQAVEP 420 I+QMHAN+R+EIKE+ AGDIAA VGLKD TTGDTL D + ++LERM+FPEPVISQAVEP Sbjct: 361 IVQMHANQREEIKEMMAGDIAAVVGLKDTTTGDTLCDDKAMVVLERMVFPEPVISQAVEP 420 Query: 421 KTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLEILVDRMKREFNVEAT 480 KTKADQEKMG+ALNRLA EDPSFRV TDEESGQTI++GMGELHL+I+VDRMKREFNVEAT Sbjct: 421 KTKADQEKMGLALNRLAAEDPSFRVRTDEESGQTIIAGMGELHLDIIVDRMKREFNVEAT 480 Query: 481 VGKPQVAYREAIRKAVEDVEGKFVKQSGGRGQYGHVVIKLEPQPAGKGYEFVDAIKGGVV 540 VGKPQVAYRE IRK E+ EGKFVKQSGGRGQYGHVV+K+EPQ AGKG+EFVDAIKGG V Sbjct: 481 VGKPQVAYRETIRKTCEESEGKFVKQSGGRGQYGHVVLKIEPQEAGKGFEFVDAIKGGTV 540 Query: 541 PREFIPAVDKGIQESLKAGVLAGYPVVDVKATLTFGSYHDVDSNENAFRMAGSMAFKEAM 600 PREFIPAV+KG++E+L GVLAGYPVVDVK TL FGSYHDVDSNENAFRMAGSMAFKE Sbjct: 541 PREFIPAVEKGVRETLNTGVLAGYPVVDVKVTLFFGSYHDVDSNENAFRMAGSMAFKEGC 600 Query: 601 KRAGPMLLEPMMQVEVETPEEFMGNVMGDLSSRRGMVQGMEDMV-GGGKLVRAEVPLSEM 659 +RA P++LEPMM VEVETPE++ G VMGDLSSRRGMVQGM+++ GGGK+++AEVPLSEM Sbjct: 601 RRASPVILEPMMAVEVETPEDYAGTVMGDLSSRRGMVQGMDEIAGGGGKIIKAEVPLSEM 660 Query: 660 FGYSTTLRSLSQGRATYSMEFKHYAEAPRQVVEQLSGGKGK 700 FGYSTTLRS +QGRATYSMEFKHY+EAP+ V++ + K K Sbjct: 661 FGYSTTLRSATQGRATYSMEFKHYSEAPKNVIDAIVTSKAK 701 >gi|300312224|ref|YP_003776316.1| peptide chain release factor 3 [Herbaspirillum seropedicae SmR1] Length = 530 Score = 175 bits (443), Expect = 1e-44, Method: Compositional matrix adjust. Identities = 139/524 (26%), Positives = 239/524 (45%), Gaps = 64/524 (12%) Query: 7 IENYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEV----HNGAATMDWMEQEQERGIT 62 ++ R GI +H DAGKTT TE++L ++G G V AT DWME E++RGI+ Sbjct: 6 VKRRRTFGIISHPDAGKTTLTEKLLLFSGAIQLAGTVKARKSGRHATSDWMEIEKQRGIS 65 Query: 63 ITSAATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQ 122 + S+ M Y +H +N++DTPGH DF+ + R + +D A+MV D+ GV+ Q Sbjct: 66 VASSV-------MQFEYRDHVVNLLDTPGHQDFSEDTYRVLTAVDSALMVIDAAKGVEEQ 118 Query: 123 SETVWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERLKGKAVPIQIPVGAEDHFSGV 182 + + P I F+NK+DR D + ++E LK + P+ P+G +F GV Sbjct: 119 TIKLLNVCRMRNTPIITFMNKLDRETRDSLELLDELESVLKIQCAPVTWPIGMGKNFRGV 178 Query: 183 IDLVKMKAIIW---DEASQGVLFKYEDI---------PLELEDTARHWRDLMVEQAAEAN 230 L++ + +++ +E + G + + I PLE+E + Sbjct: 179 YHLLRDEIMLFKAGEERANGNVEIIKGIDNPKLAEMFPLEIE---------------QLK 223 Query: 231 EELLEKYLSGNPLTEDDIKRGLRLRTVANEIVPMLAGSAFKNKGVQAMLDAVIDYLPSPV 290 E+ + + +P DD G++ P+ GSA N GV+ +LDA++D+ P P Sbjct: 224 MEVELVHGASHPFNLDDFLAGVQ--------TPVFFGSAINNFGVREILDALLDWAPGP- 274 Query: 291 DVPAIAGHGEDDSEIERHPSDEEPFSALAFKIMT--DP-FVGQLTFFRVYSGIVNSGDTV 347 G D+ + EEPFS FKI DP ++ F RV SG G + Sbjct: 275 --------GGRDATVREVKPTEEPFSGFVFKIQANMDPAHRDRIAFLRVCSGRFERGMKL 326 Query: 348 YNPVKGKKERLGRILQMHANERKEIKEVFAGDIAAAVGLKDVTTGDTLSDPEHPIILERM 407 + + ++ ++ A+ R++++E +AGDI ++ GD+ S+ E +L+ Sbjct: 327 KHLRLNRDIKVSSVVTFMASSREQVEEAYAGDIIGLPNHGNMQIGDSFSEGE---MLQFT 383 Query: 408 IFP--EPVISQAVEPKTKADQEKMGIALNRLAQEDPSFRVHTDEESGQTIMSGMGELHLE 465 P P + + +++ L +L +E + +V I+ +G L E Sbjct: 384 GIPYFAPDFFRVARIRNPLKIKQLHKGLQQLGEEG-AVQVFKPVTGSDLILGAVGVLQFE 442 Query: 466 ILVDRMKREFNVEATVGKPQVAYREAIRKAVEDVEGKFVKQSGG 509 ++ R+ E+ V+A ++ I + F K S G Sbjct: 443 VVASRLLNEYGVDAVFEGTSISSARWISCDDKKKLADFEKSSAG 486 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 97.4 bits (241), Expect = 3e-21, Method: Compositional matrix adjust. Identities = 62/183 (33%), Positives = 97/183 (53%), Gaps = 12/183 (6%) Query: 7 IENYRNIGISAHIDAGKTTTTERIL-FYTGVNHKIGEVHNGAATMDWMEQEQERGITITS 65 + N RN I AHID GK+T +RI+ G++++ E A +D M+ E+ERGITI + Sbjct: 1 MNNIRNFSIIAHIDHGKSTLADRIIQLCGGLSNREME----AQVLDSMDIERERGITIKA 56 Query: 66 AATTAFWKGMAGNYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQSET 125 +K G + +N+IDTPGHVDF+ EV RS+ +GA++V D+ GV+ Q+ Sbjct: 57 QTAALSYKAKDGQI--YNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVA 114 Query: 126 VWRQANKYKVPRIAFVNKMDRIGADFFRVQKQIEERL---KGKAVPI--QIPVGAEDHFS 180 A V + +NK+D AD + +IE+ + A P + +G ED Sbjct: 115 NCYTALDLGVEVVPVLNKIDLPSADPDNAKSEIEDVIGIDASDATPCSAKTGLGVEDVLE 174 Query: 181 GVI 183 ++ Sbjct: 175 AIV 177 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 85.5 bits (210), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 51/132 (38%), Positives = 73/132 (55%), Gaps = 9/132 (6%) Query: 18 HIDAGKTTTTERILFYTGVNHKIGEVHNGAATMDWMEQEQERGITITSAATTAFWKGMAG 77 H+D GKTT +++L +G +V N MD + E+ERGITI S +KG Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDN--RVMDSNDIEKERGITILSKNCAVEYKGT-- 85 Query: 78 NYPEHRINIIDTPGHVDFTIEVERSMRVLDGAVMVYDSVGGVQPQSETVWRQANKYKVPR 137 INI+DTPGH DF EVER + ++D +++ D+ G PQ+ V R+A + Sbjct: 86 -----HINIVDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKP 140 Query: 138 IAFVNKMDRIGA 149 I +NK+DR GA Sbjct: 141 IVVLNKIDRPGA 152 Score = 66.6 bits (161), Expect = 6e-12, Method: Compositional matrix adjust. Identities = 60/229 (26%), Positives = 101/229 (44%), Gaps = 33/229 (14%) Query: 275 VQAMLDAVIDYLPSPVDVPAIAGHGEDDSEIERHPSDEEPFSALAFKIMTDPFVGQLTFF 334 ++ + DA++ Y+P+ D P + P + +VG++ Sbjct: 200 MEPLFDAILKYVPARKDDP------------------DAPLQLQITSLEYSSYVGKIGVG 241 Query: 335 RVYSGIVNSG-DTVY---NPVKGKKERLGRILQMHANERKEIKEVFAGDIAAAVGLKDVT 390 R+ +G V G D V+ K K R+ ++L ER + E AGDI G++++ Sbjct: 242 RILAGRVKGGQDVVWMNGPDDKPTKARINQVLTFKGLERVLVDEALAGDIVLINGIEEIG 301 Query: 391 TGDTLSDPEHPIILERMIFPEPVISQAVEPKTKADQEKMGIAL------NRLAQE---DP 441 G T+ P+ P L + EP ++ T + G + +RL +E + Sbjct: 302 IGSTICSPDAPNGLPMLKVDEPTLTMNFMVNTSPLAGREGKFVTTRQIRDRLEKELKSNM 361 Query: 442 SFRVHTDEESGQTI-MSGMGELHLEILVDRMKREFNVEATVGKPQVAYR 489 + RV E T +SG GELHL IL++ M+RE E V +P+V +R Sbjct: 362 ALRVVQAENDDSTYEVSGRGELHLTILIENMRRE-GFELAVSRPRVVFR 409 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,196,768 Number of extensions: 94926 Number of successful extensions: 290 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 277 Number of HSP's successfully gapped: 8 Length of query: 701 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 609 Effective length of database: 1,184,250 Effective search space: 721208250 Effective search space used: 721208250 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 134 (56.2 bits)