BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0008.p (247 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313611|ref|YP_003777703.1| amino acid ABC transporter perm... 494 e-141 gi|300309410|ref|YP_003773502.1| glutamine ABC transporter perme... 300 9e-83 gi|300311471|ref|YP_003775563.1| amino acid ABC transporter perm... 132 2e-32 gi|300310495|ref|YP_003774587.1| amino acid ABC transporter perm... 123 2e-29 gi|300312812|ref|YP_003776904.1| glutamate/aspartate ABC transpo... 122 2e-29 >gi|300313611|ref|YP_003777703.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 247 Score = 494 bits (1273), Expect = e-141, Method: Compositional matrix adjust. Identities = 246/247 (99%), Positives = 246/247 (99%) Query: 1 MDFRWSIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKGM 60 MDFRWSIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLK M Sbjct: 1 MDFRWSIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKSM 60 Query: 61 RWLAGFYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTV 120 RWLAGFYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTV Sbjct: 61 RWLAGFYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTV 120 Query: 121 ALSLNAGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEA 180 ALSLNAGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEA Sbjct: 121 ALSLNAGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEA 180 Query: 181 ITLLKDSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESK 240 ITLLKDSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESK Sbjct: 181 ITLLKDSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESK 240 Query: 241 YSAPHRR 247 YSAPHRR Sbjct: 241 YSAPHRR 247 >gi|300309410|ref|YP_003773502.1| glutamine ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 250 Score = 300 bits (767), Expect = 9e-83, Method: Compositional matrix adjust. Identities = 149/246 (60%), Positives = 194/246 (78%), Gaps = 1/246 (0%) Query: 3 FRWSIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLK-GMR 61 F+W II Y PLF +G MTL+ ++I ++ GT GL++G+ RLAE +HG WK+ L ++ Sbjct: 5 FQWEIIGEYLPLFVEGTWMTLKAAIICVIAGTCWGLVLGVGRLAEARHGFWKYFLCYCVQ 64 Query: 62 WLAGFYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVA 121 W FYV+F RGTPLFVQI+LIHFA+MP+L+ GL+++G+LAR I+ YGAF S +A Sbjct: 65 WPIRFYVSFLRGTPLFVQILLIHFALMPMLINPSGGLILSGDLAREIRSHYGAFASAVLA 124 Query: 122 LSLNAGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAI 181 ++LN+GAY+SEIFRAGIQSI+RGQS A+ SLGM+Y T+R VVLPQAFRRMLPPLGN AI Sbjct: 125 ITLNSGAYVSEIFRAGIQSIDRGQSEASRSLGMSYLATLRRVVLPQAFRRMLPPLGNNAI 184 Query: 182 TLLKDSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKY 241 ++KDSSL SAIGLAELAYAARTV+G YARYWEPY+TISV+Y+ +T+ L+ +V LE++Y Sbjct: 185 AIVKDSSLASAIGLAELAYAARTVSGAYARYWEPYLTISVIYWLITLALSALVRHLEARY 244 Query: 242 SAPHRR 247 R Sbjct: 245 GKGDAR 250 >gi|300311471|ref|YP_003775563.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 221 Score = 132 bits (332), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 83/241 (34%), Positives = 129/241 (53%), Gaps = 27/241 (11%) Query: 6 SIIQGYAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKGMRWLAG 65 ++Q AP AKGV TL +L S+L G V+G ++ + R+ PW+ + W A Sbjct: 7 ELLQQAAPTLAKGVGYTLLFALASMLGGLVLGFVLAVARIV-----PWR----PIHWPAA 57 Query: 66 FYVAFFRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVALSLN 125 YV+ RGTPL VQI ++++ + G+ I ++ +G + LSLN Sbjct: 58 IYVSMMRGTPLLVQIFVVYYGL--------PGMGI----------EFSPLTAGVLTLSLN 99 Query: 126 AGAYISEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAITLLK 185 A AY+SE R I + RGQ AA S+G+ Y T+RY+++PQA R +P + N I+L+K Sbjct: 100 ASAYLSESLRGAILGVTRGQWNAAFSVGLTYFQTLRYIIVPQAVRIAVPSMSNTLISLIK 159 Query: 186 DSSLVSAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKYSAPH 245 D+SLVS I + EL A + V R Y+ + +Y+ +++C + RLE K H Sbjct: 160 DTSLVSVITVTELMLATKEVIAVTFRPLPLYVAAAAIYWMLSLCFEALQHRLEKKLGKAH 219 Query: 246 R 246 + Sbjct: 220 Q 220 >gi|300310495|ref|YP_003774587.1| amino acid ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 218 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 85/237 (35%), Positives = 129/237 (54%), Gaps = 27/237 (11%) Query: 11 YAPLFAKGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKGMRWLAGFYVAF 70 + P+ KG L+T++V++ S L+ +VIGLL+ +++++ LK + V Sbjct: 9 FLPILLKGALVTVEVTIYSFLLSSVIGLLLALMKVSR---------LKPVALAGATIVNI 59 Query: 71 FRGTPLFVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVALSLNAGAYI 130 RG P+ VQ+ I+F V+P I AF +G V L + AY Sbjct: 60 IRGLPIIVQLFYIYF-VLP-----------------DIGIQLSAFQAGVVGLGIAYSAYQ 101 Query: 131 SEIFRAGIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAITLLKDSSLV 190 +E FRAGI++++ GQ AA S+GM L MR VVLPQAFR LPP GN + +LKDSSLV Sbjct: 102 AENFRAGIEAVDMGQIEAAQSIGMRGPLIMRRVVLPQAFRIALPPYGNTLVMMLKDSSLV 161 Query: 191 SAIGLAELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESKYSAPHRR 247 S I + E+ A + +A + + Y T++VLY +++ L ++ RLE + S R Sbjct: 162 STITVLEMTRAGQMIASSTFQNMTVYTTVAVLYLLLSLPLVFLIRRLEIRNSKGRAR 218 >gi|300312812|ref|YP_003776904.1| glutamate/aspartate ABC transporter permease [Herbaspirillum seropedicae SmR1] Length = 227 Score = 122 bits (307), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 81/224 (36%), Positives = 121/224 (54%), Gaps = 19/224 (8%) Query: 17 KGVLMTLQVSLISILIGTVIGLLVGMLRLAEVQHGPWKWPLKGMRWLAGFYVAFFRGTPL 76 G+L++L+++L++I+ G V G L+ M+RL+ +H P ++W A YV FR PL Sbjct: 20 DGLLVSLKITLVAIVFGMVWGTLLAMMRLS--RHSP-------LQWFAAGYVNLFRSIPL 70 Query: 77 FVQIMLIHFAVMPVLVQQEHGLLITGELARTIKQDYGAFLSGTVALSLNAGAYISEIFRA 136 V ++L F ++P L+Q+ GL +L T S VA +L AY +EI RA Sbjct: 71 -VMVLLWFFLIVPQLLQKLFGLSPANDLRMT---------SALVAFALFEAAYYAEIIRA 120 Query: 137 GIQSIERGQSYAAASLGMNYGLTMRYVVLPQAFRRMLPPLGNEAITLLKDSSLVSAIGLA 196 GI S+ RGQ AA +LG+ Y M V+LPQAFR M+P L + I L +D+SLV LA Sbjct: 121 GINSVSRGQMAAAYALGLTYPQAMGMVILPQAFRNMVPLLLTQGIVLFQDTSLVYVSALA 180 Query: 197 ELAYAARTVAGTYARYWEPYITISVLYFSMTICLALVVARLESK 240 + A ++ R E +YF + ++L+V R K Sbjct: 181 DFFGRAYSIGERNGRIIEMLFVAGAVYFIICFSMSLMVKRYRKK 224 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.327 0.140 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 752,686 Number of extensions: 30790 Number of successful extensions: 212 Number of sequences better than 1.0e-08: 19 Number of HSP's gapped: 187 Number of HSP's successfully gapped: 19 Length of query: 247 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 164 Effective length of database: 1,226,865 Effective search space: 201205860 Effective search space used: 201205860 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 129 (54.3 bits)