BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0007.p (271 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/sign... 543 e-156 gi|300309409|ref|YP_003773501.1| glutamine ABC transporter perip... 206 1e-54 gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/sign... 115 3e-27 gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signa... 114 9e-27 gi|300311601|ref|YP_003775693.1| glutamine ABC transporter perip... 112 2e-26 >gi|300313610|ref|YP_003777702.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 271 Score = 543 bits (1400), Expect = e-156, Method: Compositional matrix adjust. Identities = 270/271 (99%), Positives = 270/271 (99%) Query: 1 MIKMFTRHARALALTAGFVGMLGLAACGKQEDKSAAAAAPQAAAPARVYVVGVESAYAPF 60 MIKMFTRHARALALTAGFVGMLGLAACGKQEDKSA AAAPQAAAPARVYVVGVESAYAPF Sbjct: 1 MIKMFTRHARALALTAGFVGMLGLAACGKQEDKSATAAAPQAAAPARVYVVGVESAYAPF 60 Query: 61 SSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDLLISAITITDER 120 SSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDLLISAITITDER Sbjct: 61 SSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDLLISAITITDER 120 Query: 121 KKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGDELVQQVLGKNSAKI 180 KKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGDELVQQVLGKNSAKI Sbjct: 121 KKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQSATSGDELVQQVLGKNSAKI 180 Query: 181 KRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPSFPKEDYGIAVRKD 240 KRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPSFPKEDYGIAVRKD Sbjct: 181 KRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPSFPKEDYGIAVRKD 240 Query: 241 DPELLAKINKGLAEMKADGSFAAISAQYFGK 271 DPELLAKINKGLAEMKADGSFAAISAQYFGK Sbjct: 241 DPELLAKINKGLAEMKADGSFAAISAQYFGK 271 >gi|300309409|ref|YP_003773501.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 259 Score = 206 bits (525), Expect = 1e-54, Method: Compositional matrix adjust. Identities = 107/228 (46%), Positives = 149/228 (65%), Gaps = 1/228 (0%) Query: 42 AAAPARVYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNF 101 A A + YVVG Y PF E +K++VGFDID++KA+++ +++ V TP+EG F Sbjct: 23 ALAADQTYVVGAGGTYRPFEFETPKKELVGFDIDLIKAISQASNFKIQLVSTPWEGIFAT 82 Query: 102 LAQGDRDLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQS 161 L QGDRD++IS ITITD+R + V FS PYF A Q I + AD K++ + DLK V + Sbjct: 83 LGQGDRDIIISGITITDKRAQMVDFSLPYFPAEQVI-ITNADAKITSLSDLKKTQVAVVN 141 Query: 162 ATSGDELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLR 221 +T+GD +V + LGK S I RFD+T L L+EL GGVDA V D V+K YI ++P K + Sbjct: 142 STAGDIVVSEELGKASTAIHRFDNTVLMLEELYRGGVDAAVGDVGVIKFYIKSHPEKKFK 201 Query: 222 TVTDPSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYF 269 V D F ++ +GIAV+K + ELL KIN GL ++ ADG++A I +F Sbjct: 202 VVGDSKFVRQYFGIAVKKGNKELLDKINAGLKKIVADGTYAKIYKTWF 249 >gi|300311472|ref|YP_003775564.1| amino acid ABC transporter/signal transduction system periplasmic protein/domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 267 Score = 115 bits (289), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 74/222 (33%), Positives = 125/222 (56%), Gaps = 7/222 (3%) Query: 51 VGVESAYAPFSSENEQK-DVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNFLAQGDRDL 109 V +E Y PF+ ++ + ++ GF++D+ K LA K+G++ F T + G L G D+ Sbjct: 41 VALEGNYPPFNFKDPKTGELTGFEVDVAKLLAAKLGVKPVFTTTEWSGILAGLGAGKYDV 100 Query: 110 LISAITITDERKKSVAFSEPYFVAT-QTIALPAADTKVSKMEDLKPLTVGTQSATSGDEL 168 +I+ + ITDER+K+ FS+PY +++ Q I + +EDLK +G T+ ++ Sbjct: 101 IINQVGITDERQKAFDFSDPYTLSSAQLIVRKDEKREFKSLEDLKGKKLGLGQGTNFEQK 160 Query: 169 VQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLRTVTDPSF 228 + V G + ++ + +P L +L +G +DA + D +V YI + N L+ + P Sbjct: 161 AKAVPGID---VRTYPGSPEYLADLAAGRIDAALNDSLLV-GYILKSTNLPLKAGS-PIG 215 Query: 229 PKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYFG 270 + GI RK +PE A +NK LA++KADGSF A S ++FG Sbjct: 216 AVDKIGIPFRKGNPEFKAALNKALADIKADGSFKAASEKWFG 257 >gi|300312810|ref|YP_003776902.1| glutamine ABC transporter/signal transduction system periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 249 Score = 114 bits (285), Expect = 9e-27, Method: Compositional matrix adjust. Identities = 75/229 (32%), Positives = 121/229 (52%), Gaps = 6/229 (2%) Query: 42 AAAPARVYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFEGFFNF 101 A+A A VV ++A+ PF ++ + VGFDID+ A+AK IG+ K P F G Sbjct: 21 ASARAETLVVACDTAFVPFEFKDRNQ-YVGFDIDLWDAIAKDIGVTYKLQPMDFNGIIPA 79 Query: 102 LAQGDRDLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLKPLTVGTQS 161 L + D+ ++ ITI DERKK + FS+ Y+ + ++ +P + + + DL + +S Sbjct: 80 LQTKNVDVALAGITIKDERKKVIDFSDGYYDSGFSLMVPVG-SSIKSVADLAGKALAVKS 138 Query: 162 ATSGDELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADEPVVKNYIANNPNSKLR 221 TS + + + ++ +F + A EL++G VDA + D P V YIA + + Sbjct: 139 GTSAFDYAKANF--KATELHQFPNIDNAYLELQTGRVDAAMHDTPNVLYYIATAGKGRAK 196 Query: 222 TVTDPSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAISAQYFG 270 V + YGI K P L+ K+N LA++KADG +A I ++FG Sbjct: 197 VVGTQMMAHQ-YGIGFPKGSP-LVPKVNAALAKIKADGRYAEIYRKWFG 243 >gi|300311601|ref|YP_003775693.1| glutamine ABC transporter periplasmic protein [Herbaspirillum seropedicae SmR1] Length = 251 Score = 112 bits (281), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 82/246 (33%), Positives = 123/246 (50%), Gaps = 27/246 (10%) Query: 37 AAAPQAAAPARVYVVGVESAYAPFSSENEQKDVVGFDIDIMKALAKKIGIEVKFVPTPFE 96 AA P A A V VG ++ + P + K GFD+++++ALAK +G +V+++ F+ Sbjct: 20 AAVPAAHANGEVIRVGTDATFPPMEFVKDGKRT-GFDVELIEALAKTMGKKVQWIDIDFK 78 Query: 97 GFFNFLAQGDRDLLISAITITDERKKSVAFSEPYFVATQTIALPAADTKVSKMEDLK--- 153 G L D+ SAI +TDER+K V FS+PY+ + + D + EDL Sbjct: 79 GLIPGLVSNRFDIAASAIYMTDERRKVVNFSDPYYRGGLAVLVRRDDNSIKVPEDLNKGK 138 Query: 154 --PLTVGTQSATS------GDELVQQVLGKNSAKIKRFDSTPLALKELESGGVDAVVADE 205 + VGT+S G E V+ + KN A FD L +G V+AVV Sbjct: 139 RVSVQVGTKSVGYLKDNFPGVERVE--VEKNQAM---FDL-------LATGRVNAVVTGR 186 Query: 206 PVVKNYIANNPNSKLRTVTDPSFPKEDYGIAVRKDDPELLAKINKGLAEMKADGSFAAIS 265 P Y P L V D E YG A+RK D EL A++NK L ++ +G++ A++ Sbjct: 187 PAAVEYARTQP---LVRVLDKGLTTELYGFALRKSDTELTAQLNKALQTLRMNGTYTALT 243 Query: 266 AQYFGK 271 ++FGK Sbjct: 244 DKWFGK 249 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.315 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 740,958 Number of extensions: 27501 Number of successful extensions: 138 Number of sequences better than 1.0e-08: 17 Number of HSP's gapped: 118 Number of HSP's successfully gapped: 17 Length of query: 271 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 187 Effective length of database: 1,222,130 Effective search space: 228538310 Effective search space used: 228538310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 130 (54.7 bits)