BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0006.p (690 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep ... 1420 0.0 gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Her... 313 4e-86 gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Her... 211 2e-55 >gi|300313605|ref|YP_003777697.1| ATP-dependent DNA helicase rep [Herbaspirillum seropedicae SmR1] Length = 690 Score = 1420 bits (3676), Expect = 0.0, Method: Compositional matrix adjust. Identities = 685/690 (99%), Positives = 688/690 (99%) Query: 1 MSAPSFGLNKPQSEAVHYMAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRNIAALTF 60 MSAPSFGLNKPQSEAVHYMAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRNIAALTF Sbjct: 1 MSAPSFGLNKPQSEAVHYMAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRNIAALTF 60 Query: 61 TNKAALEMQERIAKLLKDPKQAKHLTVSTFHSLGVKILRQESQHLGLKDRFSIMDSDDCF 120 TNKAALEMQERIAKLLKDPKQAKHLTVSTFHSLGVKILRQESQHLGLKDRFSIMDSDDCF Sbjct: 61 TNKAALEMQERIAKLLKDPKQAKHLTVSTFHSLGVKILRQESQHLGLKDRFSIMDSDDCF 120 Query: 121 SLVQELAVTTDKAIIRGIQNSISLWKNGLVDPDLAEKNARTEDEAQAARIYRSYVATLKA 180 SLVQELAVTTDKAIIRG+QNSISLWKNGLVDPDLAEKNARTEDEAQAARIYRSYVATLKA Sbjct: 121 SLVQELAVTTDKAIIRGVQNSISLWKNGLVDPDLAEKNARTEDEAQAARIYRSYVATLKA 180 Query: 181 YQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYLLIDEYQDTNTCQYELVKLLVTGIGKK 240 YQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYLLIDEYQDTNTCQYELVKLLVTGIGKK Sbjct: 181 YQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYLLIDEYQDTNTCQYELVKLLVTGIGKK 240 Query: 241 PMFTAVGDDDQAIYAWRGATIENLKLLGVDFPNLHLIKLEQNYRSSTRILQAANAVISNN 300 PMFTAVGDDDQAIYAWRGATIENLKLLGVDFPNLHLIKLEQNYRSSTRILQAANAVISNN Sbjct: 241 PMFTAVGDDDQAIYAWRGATIENLKLLGVDFPNLHLIKLEQNYRSSTRILQAANAVISNN 300 Query: 301 PKLFDKTLWSEHGLGDPITVMAMDDEEAEADQIAITLSAHRFERRAKFADYAILYRGNHQ 360 PKLFDKTLWSEHGLGDPITVMAMDDEEAEADQIAITLSAHRFERRAKFADYAILYRGNHQ Sbjct: 301 PKLFDKTLWSEHGLGDPITVMAMDDEEAEADQIAITLSAHRFERRAKFADYAILYRGNHQ 360 Query: 361 ARILEKSLRRERIPYVMSGGQSYFDRAEIKDIIAYLRLIANQDDDPAFIRAVTTPRRGVG 420 ARILEKSLRRERIPYVMSGGQSYFDRAEIKDIIAYLRLIANQDDDPAFIRAVTTPRRGVG Sbjct: 361 ARILEKSLRRERIPYVMSGGQSYFDRAEIKDIIAYLRLIANQDDDPAFIRAVTTPRRGVG 420 Query: 421 QATLEVLGTLAGQWQCSLFEAVYKGGLEDKLTDRQLLPLRKFCDFINALESRATRPGPSG 480 QATLEVLGTLAGQWQCSLFEAVYKGGLEDKLTDRQLLPLRKFCDFINALESRATRPGPSG Sbjct: 421 QATLEVLGTLAGQWQCSLFEAVYKGGLEDKLTDRQLLPLRKFCDFINALESRATRPGPSG 480 Query: 481 SGENAGKVLDDMMEAINYEFYLYDSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEK 540 SGENAGKVLDDMMEAINYEFYLYDSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEK Sbjct: 481 SGENAGKVLDDMMEAINYEFYLYDSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEK 540 Query: 541 NLLEITQMVALMSMLEGRDEEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTP 600 NLLEITQMVALMSMLEGRDEEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTP Sbjct: 541 NLLEITQMVALMSMLEGRDEEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTP 600 Query: 601 PEEAAARVEEERRLMYVGITRAQRSLHLSWCKRRKRAKEVIECQISRFVKEMRLDEGEAV 660 PEEAAARVEEERRLMYVGITRAQRSLHLSWCKRRKRAKEVIECQISRFVKEMRLDEGEAV Sbjct: 601 PEEAAARVEEERRLMYVGITRAQRSLHLSWCKRRKRAKEVIECQISRFVKEMRLDEGEAV 660 Query: 661 PQESEKITPQARLANLKALLQKPRPTAEEN 690 PQESEKITPQARLANLKALLQKPR T E++ Sbjct: 661 PQESEKITPQARLANLKALLQKPRNTPEQS 690 >gi|300309775|ref|YP_003773867.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 759 Score = 313 bits (801), Expect = 4e-86, Method: Compositional matrix adjust. Identities = 241/694 (34%), Positives = 355/694 (51%), Gaps = 54/694 (7%) Query: 15 AVHYMAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRNIAALTFTNKAALEMQERIAK 74 AV A L+LAGAGSGKTRV+T +IA LI+ I A+TFTNKAA EM R++ Sbjct: 15 AVTLPAQSALILAGAGSGKTRVLTTRIAWLIQTGQVSPSGILAVTFTNKAAKEMTARLSG 74 Query: 75 LLKDPKQAKHLTVSTFHSLGVKILRQESQHLGLKDRFSIMDSDDCFS----LVQELAVTT 130 +L P + + + TFH L ++LR + GL F I+D+ D S L++++ V Sbjct: 75 ML--PINTRGMWIGTFHGLCNRLLRAHHKDAGLPQTFQILDTQDQLSAIKRLLKQMNVDD 132 Query: 131 DKAIIRGIQNSISLWKNGLVDPDLAEKNARTEDEA--QAARIYRSYVATLKAYQAVDFDD 188 +K R + I N D L K+ D+ + +Y Y + VDF + Sbjct: 133 EKYPPRNLMYFI----NSAKDQGLRAKDVDAYDDYNRKFVELYELYDQQCQREGVVDFAE 188 Query: 189 LIRLPVELFRSNEEVRDRWQRKLRYLLIDEYQDTNTCQYELVKLLVTGIGKKPMFTAVGD 248 L+ EL N+ +R+ +Q + R++L+DE+QDTN QY+ +KL+ G+ AVGD Sbjct: 189 LLLRTYELLSRNQPLREHYQSRFRHILVDEFQDTNDLQYKWLKLMA---GQHNAVFAVGD 245 Query: 249 DDQAIYAWRGATIENLKLLGVDFPNLHLIKLEQNYRSSTRILQAANAVISNNPKLFDKTL 308 DDQ+IYA+RGA + N++ +F +LIKLEQNYRS IL AN +I+NN K K L Sbjct: 246 DDQSIYAFRGANVGNMQAFEQEFHVQNLIKLEQNYRSHGHILDTANELIANNSKRLGKNL 305 Query: 309 WSEHGLGDPITVMAMDDEEAEADQIAITLSAHRFERRAKFADYAILYRGNHQARILEKSL 368 ++ G G+ + V + EA I + E A+ ++ AILYR N Q+R+LE +L Sbjct: 306 RTDAGHGEQVRVYEATSDLQEAQWIIEQVKDLIAEGWAR-SEIAILYRSNAQSRVLEHAL 364 Query: 369 RRERIPYVMSGGQSYFDRAEIKDIIAYLRLIANQDDDPAFIRAVTTPRRGVGQATLEVLG 428 IPY + GGQ +F+RAEIK IAYL+L+ N +D AF+R V P RG+G +E L Sbjct: 365 FSSAIPYRVYGGQRFFERAEIKHAIAYLQLMDNLHNDSAFLRVVNFPTRGIGAKAMESLQ 424 Query: 429 TLAGQWQCSLFEAV-YKGG------------LEDKLTDRQLLPLRKFCDFINALESRATR 475 A Q+ CSL+ AV Y G +E + Q LPL + + L T Sbjct: 425 DAARQYGCSLYAAVPYVAGKAGSSLNAFVKLIEGARFETQHLPLPEMVKVVLDLSGLMTH 484 Query: 476 PGPSGSGENAGKVLDDMMEA----INYEFYLYDSFEERAAQARWQNVVDFINWLKERACG 531 G + + L+ ++ A ++ E Y D A A Sbjct: 485 YRNEKEGADRIENLEQLVSAATLFVSEEGYGLD------APAFLGPQAQATAGAAITTAD 538 Query: 532 GRDGEGEEKNLLEI-TQMVALMSM--LEGRDEE----QDAVRMSTLHASKGLEFPHVFLV 584 G + + L+ I + + A +S LE D + QDA+++ T+H++KGLEF VF+ Sbjct: 539 GVEVLDADAPLVTIMSPLSAFLSHASLEAGDNQAQAGQDALQLMTVHSAKGLEFDAVFIT 598 Query: 585 GVEEGILPHKGDPDTPPEEAAARVEEERRLMYVGITRAQRSLHLSWCKRRKRAKEVIECQ 644 G+EEG+ PH+ + VEEERRLMYV ITRA++ L +++ + R + Sbjct: 599 GMEEGLFPHENSA-----KEEGGVEEERRLMYVAITRARKRLFMTFSQTRMLHGQTRYNM 653 Query: 645 ISRFVKEMRLDEGEAVPQESEKITPQARLANLKA 678 SRF EM +++ S KI AN K+ Sbjct: 654 RSRFFDEM---PEQSIKWLSPKIAQHHWFANPKS 684 >gi|300312090|ref|YP_003776182.1| ATP-dependent DNA helicase [Herbaspirillum seropedicae SmR1] Length = 707 Score = 211 bits (537), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 203/676 (30%), Positives = 326/676 (48%), Gaps = 98/676 (14%) Query: 2 SAPSFGLNKPQSEAVHY-------MAGPCLVLAGAGSGKTRVITQKIAHLIENCGYESRN 54 S P LN Q EAV + P L++AGAGSGKT + ++A L+ G + + Sbjct: 20 SDPFARLNAQQREAVEHGGKLPPSQQPPLLIVAGAGSGKTNTLAHRVARLVLE-GADPQR 78 Query: 55 IAALTFTNKAALEMQERIAKLL--------KDPKQAKHLTVSTFHSLGVKILRQESQHLG 106 I L+F+ +AA +M R+ +++ D H + TFH++G ++LR+ ++ +G Sbjct: 79 ILLLSFSRRAAADMGRRVGQVIAQVLGLAASDTPPQLHWS-GTFHAIGARLLREYAKVIG 137 Query: 107 LKDRFSIMD---SDDCFSLVQE---LAVTTDKAIIRGIQNSISLWKNGL-VDPDLAEKNA 159 L++ F+I D S+D L++ L+ T + ++ ++++ + DL E Sbjct: 138 LEEDFTIQDRGDSEDLMGLLRHDMGLSATEKRFPLKA--TCLAIYSRAVNTRQDLGEVLQ 195 Query: 160 R-----TEDEAQAARIYRSYVATLKAYQAVDFDDLIRLPVELFRSNEEVRDRWQRKLRYL 214 + E E ++ YV + +D+DDL+ ++ + ++ ++ ++ Sbjct: 196 QHFPWCCEWERPLGELFGRYVDAKEEQHVLDYDDLLLFWADML-GDAQIAQSVGQRFDHV 254 Query: 215 LIDEYQDTNTCQYELV-KLLVTGIGKKPMFTAVGDDDQAIYAWRGATIENLKLLGVDFPN 273 L+DEYQDTN Q E++ ++ G G TAVGDD QAIY++RGAT+ N+ +DFP Sbjct: 255 LVDEYQDTNRLQAEILQRMKPDGAG----VTAVGDDAQAIYSFRGATVRNI----LDFPG 306 Query: 274 L-----HLIKLEQNYRSSTRILQAANAVISNNPKLFDKTLWSEHGLGDPITVMAMDDEEA 328 L L+ LEQNYRS+ IL A+NAVI++ P+ K LWS+ ++ + DE Sbjct: 307 LFTQPVRLLTLEQNYRSTQPILHASNAVIADAPERHVKHLWSDKPSSQKPQLVVIPDEAE 366 Query: 329 EADQIAITLSAHRFERRAKFADYAILYRGNHQARILEKSLRRERIPYVMSGGQSYFDRAE 388 +A +A + AHR E A+L+R + LE L R IP+V GG + + Sbjct: 367 QARWVANRILAHR-EEGLTLKSQAVLFRTATHSAALELELARRNIPFVKFGGLKFLESGH 425 Query: 389 IKDIIAYLRLIANQDDDPAFIRAVT-TPRRGVGQATLEVLGTLAGQWQCSLFEAVYKGGL 447 IKD++A LRL N A +RA+ P GVG AT + L + G Sbjct: 426 IKDLLALLRLAHNPSGRMAGLRALQLIP--GVGPATARKVLDLMAAAADPADALLNFGAP 483 Query: 448 EDKLTDRQLLPLRKFCDFINALESRATRPGPSGSGENAGKVLDDMMEAINYEFYL----- 502 + L + F D AL RA+ P+ D++ A ++ YL Sbjct: 484 ANI-----ALQWQAFAD-TYALMRRASASWPT-----------DILAARDW--YLPQLQE 524 Query: 503 -YDSFEERAAQARWQNVVDFINWLKERACGGRDGEGEEKNLLEIT----QMVALMSMLEG 557 YD + RAA +++ A EK L E+T + + S Sbjct: 525 RYDDAQARAAD------------IEQLAVLANGYASREKFLTELTLDPPEASSDFSGPPL 572 Query: 558 RDEEQDAVRMSTLHASKGLEFPHVFLVGVEEGILPHKGDPDTPPEEAAARVEEERRLMYV 617 RDE D + +ST+H++KG E+ V ++ V +G +P D T E A + EERRL+YV Sbjct: 573 RDE--DYLTLSTIHSAKGQEWRSVHILNVVDGCIP--SDLATGSE---AEIAEERRLLYV 625 Query: 618 GITRAQRSLHLSWCKR 633 +TRA++ LHL +R Sbjct: 626 AMTRARQYLHLVVPQR 641 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,050,824 Number of extensions: 82484 Number of successful extensions: 247 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 240 Number of HSP's successfully gapped: 4 Length of query: 690 Length of database: 1,619,870 Length adjustment: 92 Effective length of query: 598 Effective length of database: 1,184,250 Effective search space: 708181500 Effective search space used: 708181500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 134 (56.2 bits)