BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0022.0001.p (625 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300313600|ref|YP_003777692.1| methyl-accepting chemotaxis pro... 1275 0.0 >gi|300313600|ref|YP_003777692.1| methyl-accepting chemotaxis protein [Herbaspirillum seropedicae SmR1] Length = 882 Score = 1275 bits (3300), Expect = 0.0, Method: Compositional matrix adjust. Identities = 621/625 (99%), Positives = 622/625 (99%) Query: 1 LLLDGENRVIASADALWIPPGSEVPVNIDGQPRLMMHSGRQYLVRTFASEGYQGYPGPAG 60 LLLDGENRVIASADALWIPPGSEVPVNIDGQPRLMMHSGRQYLVRTFASEGYQGYPGP G Sbjct: 258 LLLDGENRVIASADALWIPPGSEVPVNIDGQPRLMMHSGRQYLVRTFASEGYQGYPGPTG 317 Query: 61 WQGQVMIPVDVAFNDSASDTLSQLAPEVAEGLLAHAHSFCPPLHDIMTAAKTIQRIVWNG 120 WQGQVMIPVDVAFNDSASDTLSQLAPEVAEGLLAHAHSFCPPLHDIMTAAKTIQRIVWNG Sbjct: 318 WQGQVMIPVDVAFNDSASDTLSQLAPEVAEGLLAHAHSFCPPLHDIMTAAKTIQRIVWNG 377 Query: 121 QVMTAGQRGEMIKLKTILDQISETGNRSNELFAQSIHDLYQTVLVSSMRDAEFTTSLMVD 180 QVMTAGQRGEMIKLKTILDQISETGNRSNELFAQSIHDLYQTVLVSSMRDAEFTTSLMVD Sbjct: 378 QVMTAGQRGEMIKLKTILDQISETGNRSNELFAQSIHDLYQTVLVSSMRDAEFTTSLMVD 437 Query: 181 LLDRNLYERSDDCRWWALTPELRSMLAQDQVSADDSAKISSILAAINRLYTVYTRLFVYD 240 LLDRNLYERSDDCRWWALTPELRSMLAQDQVSADDSAKISSILAAINRLYTVYTRLFVYD Sbjct: 438 LLDRNLYERSDDCRWWALTPELRSMLAQDQVSADDSAKISSILAAINRLYTVYTRLFVYD 497 Query: 241 RHGRIVASTGDALESVLGSHIDQTTLEAVSTLRSAQQYHVSPFGPSNLYDGRHTYIYHAA 300 RHGRIVASTGDALESVLGSHIDQTTLEAVSTLRSAQQYHVSPFGPSNLYDGRHTYIYHAA Sbjct: 498 RHGRIVASTGDALESVLGSHIDQTTLEAVSTLRSAQQYHVSPFGPSNLYDGRHTYIYHAA 557 Query: 301 IRALDQDAVVGGIGIVFDAEPELRNMLVAGLSGKQRMSAFYVSRDGRILSSTDAARPVGG 360 IRALDQDAVVGGIGIVFDAEPELRNMLVAGLSGKQRMSAFYVSRDGRILSSTDAARPVGG Sbjct: 558 IRALDQDAVVGGIGIVFDAEPELRNMLVAGLSGKQRMSAFYVSRDGRILSSTDAARPVGG 617 Query: 361 TLEIDATMLQLDHGAHRSGVTLHDGQYAIVATSASSGYREFKTIDGYQEDVIAVVFQCFG 420 TLEIDATMLQLDHGAHRSGVTLHDGQYAIVATSASSGYREFKT DGYQEDVIAVVFQCFG Sbjct: 618 TLEIDATMLQLDHGAHRSGVTLHDGQYAIVATSASSGYREFKTSDGYQEDVIAVVFQCFG 677 Query: 421 AVNEGGTSNSSSAFQLEQDVAVNRNGEDLEYATFFSDHSLFAIAAVHVMEAVPYSEVLPT 480 A+NEGGTSNSSSAFQLEQDVAVNRNGEDLEYATFFSDHSLFAIAAVHVMEAVPYSEVLPT Sbjct: 678 AINEGGTSNSSSAFQLEQDVAVNRNGEDLEYATFFSDHSLFAIAAVHVMEAVPYSEVLPT 737 Query: 481 SMGSRPEQIGILGLEREGQHRDFIWVFDLGFLIRGTRSAITSSSQVMIVQHGAHTIGLLA 540 SMGSRPEQIGILGLEREGQHRDFIWVFDLGFLIRGTRSAITSSSQVMIVQHGAHTIGLLA Sbjct: 738 SMGSRPEQIGILGLEREGQHRDFIWVFDLGFLIRGTRSAITSSSQVMIVQHGAHTIGLLA 797 Query: 541 DELHAVPQFGQAQIIHTPFALQGESMLVSHVIKANGGQLLIQAIAVDRLFAWVVDGSVPH 600 DELHAVPQFGQAQIIHTPFALQGESMLVSHVIKANGGQLLIQAIAVDRLFAWVVDGSVPH Sbjct: 798 DELHAVPQFGQAQIIHTPFALQGESMLVSHVIKANGGQLLIQAIAVDRLFAWVVDGSVPH 857 Query: 601 PNALELVPQDTGAAPPPQVEALLAA 625 NALELVPQDTGAAPPPQVEALLAA Sbjct: 858 ANALELVPQDTGAAPPPQVEALLAA 882 Score = 59.3 bits (142), Expect = 9e-10, Method: Compositional matrix adjust. Identities = 87/376 (23%), Positives = 156/376 (41%), Gaps = 47/376 (12%) Query: 76 SASDTLSQLAPEVAEGLLAHAHSFCPPLHDIMTAAKTIQRIVWNGQVMTAGQRGEM-IKL 134 +A+D L PE H +F P + D+ +++Q + +++ A + + Sbjct: 8 NATDKDRILPPET------HTETFLPYMRDVARCEQSLQELNQMWRIIEASAKMNCPAEA 61 Query: 135 KTILDQISET----GNRSNELFAQSIHDLYQTVLVSSMRDAEFTTSLMVDLLDRNLYERS 190 K+IL ++ T EL + H+ + VL A + ++D++ RNL+ER+ Sbjct: 62 KSILPTMAATRSGFSRLERELVSSLAHEKVRNVLAEIGTKARY----VIDIVVRNLFERT 117 Query: 191 DDCRWWALTPELRSMLAQDQVSADDSAKISSI-LAAINRLYTVYTRLFVYDRHGRIVAST 249 D + A EL + +A DS + + L YTVY + + D G ++ Sbjct: 118 ADVGFLATDRELCNFVA----GLTDSVEATRYRLREYRNKYTVYDEIILLDTEGNVLVQI 173 Query: 250 GDA--LESVLGSHIDQTTLEAVSTLRSAQQYHVSPFGPSNLYDGR-HTYIYHAAIRALDQ 306 LE I Q TLE+ + +V F ++L + +Y + Sbjct: 174 DPTTPLEHSADPLIRQ-TLESDT--------YVETFRATDLRPAKERALVYSRRMHHPQT 224 Query: 307 DAVVGGIGIVFDAEPELRNMLVAGLSGKQRMSAFYVSRDGRILSSTDAAR-PVGGTLEID 365 AV+G + + F E E+ + A + R + + + R+++S DA P G + ++ Sbjct: 225 GAVIGVLCLCFHFEEEMAGIFAAHGDPQGRSNMLLLDGENRVIASADALWIPPGSEVPVN 284 Query: 366 ATMLQLDHGAHRSGVTLHDGQYAIVATSASSGYREFKTIDGYQEDVIAVVFQCFGAVNEG 425 G R + +H G+ +V T AS GY+ + G+Q V+ V F Sbjct: 285 ID------GQPR--LMMHSGRQYLVRTFASEGYQGYPGPTGWQGQVMIPVDVAF------ 330 Query: 426 GTSNSSSAFQLEQDVA 441 S S + QL +VA Sbjct: 331 NDSASDTLSQLAPEVA 346 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,039,405 Number of extensions: 87139 Number of successful extensions: 249 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 247 Number of HSP's successfully gapped: 2 Length of query: 625 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 534 Effective length of database: 1,188,985 Effective search space: 634917990 Effective search space used: 634917990 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 133 (55.8 bits)