BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0097.p (283 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310365|ref|YP_003774457.1| galactose-1-epimerase [Herbaspi... 151 5e-38 >gi|300310365|ref|YP_003774457.1| galactose-1-epimerase [Herbaspirillum seropedicae SmR1] Length = 324 Score = 151 bits (382), Expect = 5e-38, Method: Compositional matrix adjust. Identities = 97/279 (34%), Positives = 148/279 (53%), Gaps = 22/279 (7%) Query: 12 LGGAIASASWRGQ----PVLRPSVEAQLQQGLVRRSACYPLLPFSNRIGNAQFAFDGQTY 67 LGGA+A W+ + P+LRP + +AC+PLLP+SNRI F FDG+ Y Sbjct: 35 LGGALAGWEWQRERQWLPLLRP---WSADPAALYATACFPLLPWSNRITGGGFDFDGKHY 91 Query: 68 ALIANFDNEPHAIHGLGFQRAWQVQSSSAESLTMQLTHASPSPGQWPFALRATQVIAIEG 127 L+ N EP+ IHG +++AW + + + + +T++L + G P+ RA Q++ + Sbjct: 92 PLVPNRLGEPYPIHGDSWRQAWTITAQAEDRMTLEL-ESDRYDGN-PYVYRARQLLTLRE 149 Query: 128 DDLVLRLEVENTDHRRAPCGLGWHPFFPLDSAAQPTQLQTHWQAMLVNGPDKLPCGST-- 185 + L + L V NT P GLG HP+F D+ T+LQ + + ++GPD++P T Sbjct: 150 NALEIELRVTNTGTETLPYGLGLHPYFLRDAQ---TRLQFRSRGVWLSGPDRIPTSFTRQ 206 Query: 186 --APPDTTQ---LDTLVIDNCFTGWSGQAVVTGPHHRITLTASPTLRC--AVLFRPPGQP 238 A D + L+ +IDNCF GW G A++ P + L T R ++FRP Q Sbjct: 207 LPAGWDYNRPATLEDKLIDNCFEGWDGLAMIEDPTRGLRLQMHTTDRSGYVLMFRPVKQA 266 Query: 239 FFAFEPVSHPNNALHG-VAPAMHILEPGQCLAGEMRLSL 276 FF EPV+HP +A H P + L PG+ + R S+ Sbjct: 267 FFCLEPVTHPIDAFHRPKRPGLIDLAPGEDMVLLTRFSV 305 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.134 0.430 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 993,328 Number of extensions: 42729 Number of successful extensions: 103 Number of sequences better than 1.0e-08: 1 Number of HSP's gapped: 99 Number of HSP's successfully gapped: 1 Length of query: 283 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 199 Effective length of database: 1,222,130 Effective search space: 243203870 Effective search space used: 243203870 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)