BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0093.p (519 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312966|ref|YP_003777058.1| major facilitator superfamily p... 979 0.0 gi|300310323|ref|YP_003774415.1| methylenomycin A resistance pro... 154 2e-38 gi|300309872|ref|YP_003773964.1| major facilitator superfamily p... 129 4e-31 gi|300314020|ref|YP_003778112.1| major facilitator superfamily p... 114 2e-26 gi|300311185|ref|YP_003775277.1| major facilitator superfamily p... 112 6e-26 >gi|300312966|ref|YP_003777058.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 544 Score = 979 bits (2532), Expect = 0.0, Method: Compositional matrix adjust. Identities = 515/519 (99%), Positives = 515/519 (99%) Query: 1 MSLRLSTSAGRILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFA 60 MSLRLSTSAGRILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFA Sbjct: 26 MSLRLSTSAGRILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFA 85 Query: 61 ALLLPAGATADRYGRRRVLLGGIALFALTSLACALAPSAQALYGAPALQGVGAAFLLAPA 120 ALLLPAGATADRYGRRRVLLGGIALFALTSLACALAPSAQALYGA ALQGVGAAFLLAPA Sbjct: 86 ALLLPAGATADRYGRRRVLLGGIALFALTSLACALAPSAQALYGARALQGVGAAFLLAPA 145 Query: 121 LAVIGHGFHDEDQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLA 180 LAVIGH FHDEDQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLA Sbjct: 146 LAVIGHAFHDEDQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLA 205 Query: 181 VAVWRHVPESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALL 240 VAVWRHVPESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALL Sbjct: 206 VAVWRHVPESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALL 265 Query: 241 LVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAW 300 LVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAW Sbjct: 266 LVLFIAWQRRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAW 325 Query: 301 QTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTA 360 QTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTA Sbjct: 326 QTGLAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTA 385 Query: 361 LGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLADTA 420 LGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLADTA Sbjct: 386 LGMAVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLADTA 445 Query: 421 RHAMQNALAALDASASAAAFVERAMAGDLAQAPALLPQHGQAAAMLARQAYAQGFAHAFA 480 RHAMQNALAALDASASAAAFVERAMAGDLAQA ALLPQHGQAA MLARQAYAQGFAHAFA Sbjct: 446 RHAMQNALAALDASASAAAFVERAMAGDLAQAAALLPQHGQAAVMLARQAYAQGFAHAFA 505 Query: 481 AAALLAVATALLVLWSIRRTARPAARPGAAARLRRPPGA 519 AAALLAVATALLVLWSIRRTARPAARPGAAARLRRPPGA Sbjct: 506 AAALLAVATALLVLWSIRRTARPAARPGAAARLRRPPGA 544 >gi|300310323|ref|YP_003774415.1| methylenomycin A resistance protein [Herbaspirillum seropedicae SmR1] Length = 478 Score = 154 bits (389), Expect = 2e-38, Method: Compositional matrix adjust. Identities = 110/297 (37%), Positives = 151/297 (50%), Gaps = 11/297 (3%) Query: 17 SFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRR 76 + G M +LD VV + L +A L A S + W+V Y L FAA+LL GA ADR+G R Sbjct: 36 AIGFVMAMLDVTVVNVGLSNIAGSLDAPLSTLVWIVDGYTLTFAAMLLVGGALADRFGAR 95 Query: 77 RVLLGGIALFALTSLACALAPSAQALYGAPALQGVGAAFLLAPALAVIGHGFHDEDQRER 136 R+ L G+ LF L SL C AP+ L A LQG+GAAF + +L+++ H + D+ R R Sbjct: 96 RLYLTGLFLFVLASLLCGAAPNGPFLIVARLLQGLGAAFFMPSSLSLLTHVYEDDRVRAR 155 Query: 137 AWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPLPR 196 +W +GL + PL+GGV+ LGWR F +N+P L+ V + +P+ R Sbjct: 156 MLGVWSATVGLAAAVGPLVGGVLIHWLGWRSVFLINVPVGLVGLVMARKLIPQVGGH-AR 214 Query: 197 PLDPLGILSSSSAVFLLTWALITGPEHGWTSVAC-----VARGLGGALLLVLFIAWQRRA 251 L+ L+ + L++ LI GP +GWTS VA G AL I + R Sbjct: 215 ALNLSSHLAGVVMLAGLSFVLIQGPVYGWTSPRILAGLAVALSAGAAL-----IRHELRG 269 Query: 252 AHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLP 308 P+L +LF F AA F L LFLQ RG A QTGL ++P Sbjct: 270 IAPLLPKALFATPQFAAANGIGFLINFGVYGNLFYLALFLQQGRGADALQTGLQLVP 326 >gi|300309872|ref|YP_003773964.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 493 Score = 129 bits (325), Expect = 4e-31, Method: Compositional matrix adjust. Identities = 131/410 (31%), Positives = 187/410 (45%), Gaps = 23/410 (5%) Query: 22 MTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRRRVLLG 81 M V D ++ + LP +++ L S + WV ++Y+L A L A + R G R Sbjct: 40 MAVFDGTIINVALPQISQALQTSAAVSIWVSNAYLLATAMTLAAFAALSSRIGFRAQFSA 99 Query: 82 GIALFALTSLACALAPSAQALYGAPALQGVGAAFLLAPALAVIGHGFHDEDQRERAWALW 141 G+A+F L SL CAL+ S L LQG+G A +L+ A A+ F + L Sbjct: 100 GLAVFTLASLGCALSGSVTLLILMRILQGLGGAAMLSIAPAIHRTIFPNR--------LL 151 Query: 142 GGIMGLTMVLA-------PLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPL 194 G I+GL VL P++GG + +LGW W FA+N+P +L W VP++R P Sbjct: 152 GRILGLNAVLVAASTAVGPVLGGTLLAMLGWEWIFAINVPLGVLAVWLAWNAVPDTRKPA 211 Query: 195 PRPLDPLGILSSSSAVFLL-----TWALITGPEHGWTSVACVARGLGGALLLVL-FIAWQ 248 D G L S+ A+ L T A P G + A ALL L F+ Q Sbjct: 212 QAAFDLAGALWSALAMGALIMAADTCARFAEPGQGSGAAFTAAAYAVTALLAGLAFVRGQ 271 Query: 249 RRAAHPMLELSLFRHAGFVAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTGLAMLP 308 RRAA P+L L +F + F A A + LP QNA G +A+++ P Sbjct: 272 RRAADPLLPLDIFANPRFSLAALTSLASFVGQGIAFVALPFLFQNAYGYTAFESAALFTP 331 Query: 309 FALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTALGMA-VLG 367 + L ++L+ RLA SP +L+ L+++ A W A V G Sbjct: 332 WPLGIVLVAPHAGRLAD-RHSPALLSSLGLALFALGLVLLALLPDQAQPWDIGWRALVCG 390 Query: 368 SGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLA 417 G G + ++GNV RER+G ASG+ R G G A LG +LA Sbjct: 391 MGFGFFQSPNNREMLGNVSRERSGNASGVLAIMRTFGQCLGAALLGVILA 440 >gi|300314020|ref|YP_003778112.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 475 Score = 114 bits (286), Expect = 2e-26, Method: Compositional matrix adjust. Identities = 123/401 (30%), Positives = 186/401 (46%), Gaps = 15/401 (3%) Query: 10 GRILLTGSFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGAT 69 GR +L + LDT + LP +A DL + + W+V++Y L LLP A Sbjct: 30 GRAILALGIAVGLATLDTAIANTALPAMALDLQTTPAASVWIVTAYQLAMMVALLPLAAL 89 Query: 70 ADRYGRRRVLLGGIALFALTSLACALAPSAQALYGAPALQGVGAAFLLAPALAVIGHGFH 129 + G RR+ + G+ LF SL CA+A S L A LQG+G A +++ A+I + Sbjct: 90 GEIIGYRRIYIWGLVLFTAASLLCAVAWSLPTLVMARFLQGLGGAGIMSVNTALI-RFIY 148 Query: 130 DEDQRERAWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLL---LAVAVWRH 186 R L I+ + + P + + + W W FAVN+P +L LAV H Sbjct: 149 PTRSLGRGVGLNTLIVAIAFTVGPSVASGILAIAPWPWLFAVNVPLGVLAVVLAVPSLPH 208 Query: 187 VPESRNPLPRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIA 246 +R+ D L +++A LL A+ G SV V L A+L LF+ Sbjct: 209 TSLARH----AFDLRSALLNAAAFGLLILAIGEGAHQAQASV--VGLELAAAILCGLFL- 261 Query: 247 WQRRAAH--PMLELSLFRHAGF-VAAVAAMFAYAASAQVMASLLPLFLQNARGLSAWQTG 303 +R +H PML + LFRH F ++AV A+ ++AA S LP F + G S + G Sbjct: 262 LKRELSHPAPMLPVDLFRHPMFSLSAVTAVCSFAAQGLAFVS-LPFFFHHDLGRSQVEIG 320 Query: 304 LAMLPFALAMLLLPQVGSRLARWLSSPQMLALGLGVTALGNLLMIQAALSGAAAWTALGM 363 L M P+ +A+ ++ + R++ + +GL + G L M S + + M Sbjct: 321 LLMTPWPVAVAIMAPIAGRMSDRYPVGMLGGVGLALLCAGLLSMTFMPASPSVWEISWRM 380 Query: 364 AVLGSGGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTG 404 + G G G KA+M + P R+G ASGI TAR G Sbjct: 381 LLCGMGFGFFQSPNLKALMTSAPPHRSGGASGIVATARLLG 421 >gi|300311185|ref|YP_003775277.1| major facilitator superfamily permease [Herbaspirillum seropedicae SmR1] Length = 532 Score = 112 bits (281), Expect = 6e-26, Method: Compositional matrix adjust. Identities = 133/496 (26%), Positives = 208/496 (41%), Gaps = 20/496 (4%) Query: 17 SFGCAMTVLDTNVVGIILPTVARDLGASFSQIEWVVSSYVLCFAALLLPAGATADRYGRR 76 F + LD +VG LPT+ +L F WV ++Y+L + G D +GR+ Sbjct: 28 CFVIMLVALDQTIVGTALPTIVAEL-KGFELYAWVATAYLLTSVITVPIFGRLGDYFGRK 86 Query: 77 RVLLGGIALFALTSLACALAPSAQALYGAPALQGVGAAFLLAPALAVIGHGFHDEDQRER 136 ++ I +F S C +A S L A LQG+G L+ A + F D R R Sbjct: 87 PFVIASIIVFVGASALCGMANSMLFLVLARGLQGIGGGMLVGTCFASVADLFPDPRVRLR 146 Query: 137 AWALWGGIMGLTMVLAPLIGGVVNTLLGWRWAFAVNLPACLLLAVAVWRHVPESRNPL-- 194 + G+ + P +GG + LGWRW F NLP ++ VWR VP R+ Sbjct: 147 WQIILSVSFGIATAVGPSLGGFLTQGLGWRWVFYCNLPIGVISLFFVWRFVPHIRHMQHA 206 Query: 195 -PRPLDPLGILSSSSAVFLLTWALITGPEHGWTSVACVARGLGGALLLVLFIAWQRRAAH 253 LD G L S ++ L + P+ G TS V + A L+ W++RAA Sbjct: 207 GKMRLDWPGALLISLSLGSLQLLVELLPKRGVTSGMLVLLAVSVAAFYALW-KWEQRAAQ 265 Query: 254 PMLELSLFRHAGFVAAVAAMFAYAASAQV-MASLL---PLFLQNARGLSAWQTGLAMLPF 309 P+L + R A+A++F + A M S+L PL Q GLS + GL + P Sbjct: 266 PILPFEMLRD----RALASLFLLSVLAGFAMFSMLMYAPLLFQGGFGLSPREAGLLITPL 321 Query: 310 ALAMLLLPQVGSRLARWLSSPQ-MLALGLGVTALGNLLMIQAALSGAAAWTALGMAVLGS 368 + + V R+ + +P M+ LG + A L ++ + M G Sbjct: 322 VACITVASIVNGRIITRIPNPNLMMNLGFALIAASCLGIVLCDRVTGKGFLLAVMLAGGF 381 Query: 369 GGGLLNGQTQKAIMGNVPRERAGMASGISTTARFTGVLAGFAGLGAVLADTARHAMQNAL 428 G GL+ RE G+A+ + + R G + G A G +++ ++ AL Sbjct: 382 GLGLVLPNLTVFAQQAAAREHLGIATALLQSLRMIGGMLGTAITGTLVSQMYGAGVEKAL 441 Query: 429 AALDASASAAAFVERAMAGDLAQAPALLPQHGQA----AAMLARQAYAQGFAHAFAAAAL 484 A+ F + + + LL Q QA +A+L +A + A Sbjct: 442 EGDHAAQWLKDFSDPEVLVNHDIQSVLLAQLMQAGHDGSALL--EAAREALVGAIHMGIA 499 Query: 485 LAVATALLVLWSIRRT 500 +A+ AL+ LW +RR Sbjct: 500 IAIVAALVGLWMVRRV 515 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.325 0.135 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,248,465 Number of extensions: 49816 Number of successful extensions: 466 Number of sequences better than 1.0e-08: 22 Number of HSP's gapped: 443 Number of HSP's successfully gapped: 22 Length of query: 519 Length of database: 1,619,870 Length adjustment: 89 Effective length of query: 430 Effective length of database: 1,198,455 Effective search space: 515335650 Effective search space used: 515335650 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 133 (55.8 bits)