BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0092.p (300 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312965|ref|YP_003777057.1| LysR family transcription regul... 581 e-167 gi|300312750|ref|YP_003776842.1| LysR family transcription regul... 151 6e-38 gi|300313102|ref|YP_003777194.1| LysR family transcription regul... 127 1e-30 gi|300312961|ref|YP_003777053.1| transcription regulator protein... 125 3e-30 gi|300310637|ref|YP_003774729.1| LysR family transcription regul... 120 2e-28 >gi|300312965|ref|YP_003777057.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 581 bits (1498), Expect = e-167, Method: Compositional matrix adjust. Identities = 299/300 (99%), Positives = 299/300 (99%) Query: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA Sbjct: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 Query: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ Sbjct: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 Query: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK 180 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK Sbjct: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK 180 Query: 181 PGAIRSRALAGEDFILPEQELGSREVWRRGGFAPRRVSRPRGLLAVLAEVAVGAGVAVVP 240 PGAIRSRALAGEDFILPEQELGSREVWRRGGFAPRRVSRP GLLAVLAEVAVGAGVAVVP Sbjct: 181 PGAIRSRALAGEDFILPEQELGSREVWRRGGFAPRRVSRPGGLLAVLAEVAVGAGVAVVP 240 Query: 241 GVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERAPAVRNMIAALRASKPVQVQAATMG 300 GVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERAPAVRNMIAALRASKPVQVQAATMG Sbjct: 241 GVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERAPAVRNMIAALRASKPVQVQAATMG 300 >gi|300312750|ref|YP_003776842.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 322 Score = 151 bits (382), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 102/268 (38%), Positives = 140/268 (52%), Gaps = 9/268 (3%) Query: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 ++LR LRYF+ VAEE H +AAE +GI P LS QI+ LER+L LF R R + LT + Sbjct: 19 IDLRLLRYFIAVAEEGHLTKAAERIGIQQPPLSQQIRVLERELGVTLFNRLPRGMELTDS 78 Query: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 G ++ ARA+LA+ + + + RR +G+MGR+ VG+ SA + + AFR P Sbjct: 79 GAALLIDARALLAQLDTTVASVRRIAQGKMGRIAVGFTESASLHPFVHSVIRAFRADAPD 138 Query: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALAS- 179 V + E +L AL +LD+AFVR PV L +L + ALP DH L Sbjct: 139 VAMTVEESNTNELIEALRGKRLDLAFVRSPVGDSGGLTIETMLEEDMVAALPIDHPLVDG 198 Query: 180 -KPGAIRSRALAGEDFILPEQELGSR------EVWRRGGFAPRRVSRPRGLLAVLAEVAV 232 K +I ALA E FI+ + G GF+P+ + R L+ L+ VA Sbjct: 199 GKRKSIALHALAEESFIMTRRPNGPGLYDTIIAACHAAGFSPQVIQEARKNLSTLSLVAA 258 Query: 233 GAGVAVVPGVLTTVVNLPNVSYRALAGA 260 G GV V+P +T VN V Y L GA Sbjct: 259 GVGVTVIPASMTR-VNAEGVVYLKLTGA 285 >gi|300313102|ref|YP_003777194.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 127 bits (319), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 94/288 (32%), Positives = 146/288 (50%), Gaps = 11/288 (3%) Query: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 +ELRHL YF TVAE LHF RAAE+L I+ P L+ QI LE++L AQLF R KRAI LT+A Sbjct: 2 IELRHLTYFRTVAETLHFGRAAELLHISQPPLTRQIAALEKELGAQLFDRSKRAIQLTAA 61 Query: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 G+ F + E+A + G+ G ++VG++ S + IL + +P+ Sbjct: 62 GKYFYRDTTEIFKALERAKRNVSSSSTGKSGALKVGFMMSTAY-NILPSVTRHYSAAYPE 120 Query: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK 180 V + E+ L +E+ K+D+ + P + L SH + + LP DH LA + Sbjct: 121 VDMRLTEYVPNLLATDIEDEKIDVGIMYRPEDC-RRLDSHTIYAEPLLAVLPRDHRLAGR 179 Query: 181 PGAIRSRALAGEDFILPEQELGS------REVWRRGGFAPRRVSRPRGLLAVLAEVAVGA 234 +I + LA + FI + + ++ + GF PR V ++ V G Sbjct: 180 -QSISAAELAEDAFISIPRMIAPVVYDLIQQHCQLHGFRPRIVLETNLQQTIVNLVGEGL 238 Query: 235 GVAVVPGVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERAPAVRNM 282 GVA+VP + ++L ++ L GA +V EVA ++ + P +R Sbjct: 239 GVALVPTSM-QAMHLDTTVFKPLIGAPMV-EVAVIWNKDNTNPCIRTF 284 >gi|300312961|ref|YP_003777053.1| transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 300 Score = 125 bits (315), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 92/282 (32%), Positives = 139/282 (49%), Gaps = 10/282 (3%) Query: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 MELRHL+YF VA L F+RAAE+L IA P LS QI+QLE L +L R R IALT A Sbjct: 1 MELRHLKYFNAVASTLSFSRAAELLHIAQPPLSRQIRQLEEMLGVELIDRASRPIALTMA 60 Query: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 G+ F Q VL+R EQ + R RG +G+V SA++ G+L + + FR P+ Sbjct: 61 GKFFYEQTLQVLSRLEQIEEGTRVIARGRQHWFNIGFVPSALY-GLLPEMIKRFRTEMPE 119 Query: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK 180 + + E ++ AL+ G++D+ F RLP+ + ++ + P H L ++ Sbjct: 120 IEIGFSEIVTMEQVEALKSGRIDVGFGRLPIS-DPDIVCETIVEEPLVAVFPLGHPLLAR 178 Query: 181 PGAIRSRALAGEDFIL------PEQELGSREVWRRGGFAPRRVSRPRGLLAVLAEVAVGA 234 + LA + FIL P +++ P V + + VA G Sbjct: 179 -NKVTLAQLAAQRFILYPARPRPSYADQVLDIFSSRALRPSIVKEANEMQTAIGLVAAGV 237 Query: 235 GVAVVPGVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERA 276 GV +VP + ++ +V YR L+ V S V +R +R+ Sbjct: 238 GVTLVPRSVQG-LHRSDVVYRPLSNKGVHSPVIMNFRANDRS 278 >gi|300310637|ref|YP_003774729.1| LysR family transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 326 Score = 120 bits (300), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 91/307 (29%), Positives = 146/307 (47%), Gaps = 14/307 (4%) Query: 1 MELRHLRYFLTVAEELHFARAAEVLGIAPPTLSVQIQQLERQLQAQLFVRGKRAIALTSA 60 M++R L+YF+ VA +F RA+E L IA P LS QIQ LE +L QL +R R + LT A Sbjct: 1 MDIRQLKYFVAVANTRNFTRASEQLHIAQPPLSRQIQLLEEELGVQLLLRNSRPLRLTEA 60 Query: 61 GEDFVVQARAVLARFEQAIDAGRRAGRGEMGRVEVGYVGSAVFGGILQQQLSAFRQRWPQ 120 G F QA +L R +Q +A ++ G + + +G+V S ++GG L + RQ +P+ Sbjct: 61 GRVFYEQALQILNRLDQLKNATQQIGLNQRQTLSIGFVASTLYGG-LPMLVRKLRQHYPE 119 Query: 121 VLVHAREWPMLQLGAALEEGKLDIAFVRLPVPLGQTLQSHVLLRDRFCLALPTDHALASK 180 V + E +Q AAL+ G++D+ F R+ T+ VL ++ LA+P ALA Sbjct: 120 VDIQLLELTSMQQFAALKSGRIDVGFGRIRSN-DATVARIVLREEKLVLAIPPSFALAGS 178 Query: 181 PGAIRSRALAGEDFIL----PEQELGSR--EVWRRGGFAPRRVSRPRGLLAVLAEVAVGA 234 + + + G+ I+ P + P V R + L VA + Sbjct: 179 NQRVSLKEVHGQKLIVYPKEPRPSFADHILSLLNDQDIHPAEVHEVREIQTALGLVAAES 238 Query: 235 GVAVVPGVLTTVVNLPNVSYRALAGAAVVSEVAAVYRRFERA---PAVRNMIAALRASKP 291 G+ ++P +L YR + A S + +R + A AV+ ++A + A P Sbjct: 239 GICLIPASARLRNDL---HYRLIDDARATSPIILTHRINDNAWYIEAVKRLVADMYAEHP 295 Query: 292 VQVQAAT 298 + T Sbjct: 296 AWLDIDT 302 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.324 0.137 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 699,517 Number of extensions: 24055 Number of successful extensions: 190 Number of sequences better than 1.0e-08: 31 Number of HSP's gapped: 180 Number of HSP's successfully gapped: 31 Length of query: 300 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 215 Effective length of database: 1,217,395 Effective search space: 261739925 Effective search space used: 261739925 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 130 (54.7 bits)