BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0090.p (429 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylas... 871 0.0 gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylas... 233 3e-62 gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herba... 130 2e-31 >gi|300312963|ref|YP_003777055.1| o-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 429 Score = 871 bits (2250), Expect = 0.0, Method: Compositional matrix adjust. Identities = 428/429 (99%), Positives = 428/429 (99%) Query: 1 MADNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 M DNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR Sbjct: 1 MPDNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 Query: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF Sbjct: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 Query: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV Sbjct: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 Query: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR Sbjct: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKR 240 Query: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI Sbjct: 241 LNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRI 300 Query: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF 360 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF Sbjct: 301 TANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGGARF 360 Query: 361 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA 420 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA Sbjct: 361 QDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDDLLA 420 Query: 421 DLEQALQSA 429 DLEQALQSA Sbjct: 421 DLEQALQSA 429 >gi|300312582|ref|YP_003776674.1| O-acetylhomoserine sulfhydrylase [Herbaspirillum seropedicae SmR1] Length = 413 Score = 233 bits (594), Expect = 3e-62, Method: Compositional matrix adjust. Identities = 136/431 (31%), Positives = 223/431 (51%), Gaps = 21/431 (4%) Query: 1 MADNPQWKFETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSR 60 M D + F T +H ++ PI+ +V F ++D + A++F K G Y R Sbjct: 1 MNDKKTYGFTTTILHSDRQKGIEHGSLHKPIHTSVTFGYEDARQLAEVFQGKQPGYRYGR 60 Query: 61 IMNPTQDVLEKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLF 120 NPT LE ++ +E G + A+G AA+ +Q + GD++VS++ L+G T +L+ Sbjct: 61 QGNPTVAALEDKITKMEDGKSTICFATGMAAIGAIVQGLLREGDHVVSSAFLFGNTNSLW 120 Query: 121 AHTLPQYGVQTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGV 180 T+ G + D + E I T+ +++E+I NP + D+ + ++ G+ Sbjct: 121 M-TVGAQGAKVSMVDATDVKNVEAAITANTRLVFVETIANPRTQVADLKRIGELCRERGI 179 Query: 181 PLIVDNTVATPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKH---KQR 237 +VDNT+ +PYL RP GA +VV+SLTK +GGHG LGGA+ D+G+F W ++ + Sbjct: 180 LYVVDNTMTSPYLFRPKTVGAGLVVNSLTKSIGGHGNALGGALTDTGEFDWTRYPHIAEN 239 Query: 238 FKRLNEPDVSYHGVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRL 297 +K+ P + + R LR+ G +L P A I G ET+ LR Sbjct: 240 YKKNPAPQWG---------------MAQIRAKALRDFGGSLGPEAAHHIAVGAETIALRQ 284 Query: 298 DRITANTLAVAQYLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLTFGVANGREGG 357 +R N LA+AQ L+ +V V Y GLE HP HAL++ F+ S +++F + +G + Sbjct: 285 ERECKNALALAQMLQADERVAAVYYPGLESHPQHALSKALFRSFGS-LMSFELKDGID-C 342 Query: 358 ARFQDALQLFTRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTEDTVRLSIGIEHIDD 417 + + L+L N+GD ++L A T ++ G+ E +R+S+G+E DD Sbjct: 343 FDYLNRLRLAIPTSNLGDTRTLVIPVAHTIFYEMGAERRASMGIAESLIRVSVGLEDTDD 402 Query: 418 LLADLEQALQS 428 L+AD QAL + Sbjct: 403 LVADFRQALDA 413 >gi|300312294|ref|YP_003776386.1| cystathionine beta-lyase [Herbaspirillum seropedicae SmR1] Length = 392 Score = 130 bits (328), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 116/420 (27%), Positives = 179/420 (42%), Gaps = 45/420 (10%) Query: 10 ETISVHGGYDPDPTTRAVAVPIYQTVAFAFDDTQHGADLFDLKVQGNIYSRIMNPTQDVL 69 +T VH Y A V I+ F D + G Y PT VL Sbjct: 12 QTSLVHSSYRAPDGFEAFPVGIHHASTVLFPDVAAMRSRDWREKLGYTYGLHGTPTSFVL 71 Query: 70 EKRLAALEGGIGALALASGQAAVTYAIQTIAEAGDNIVSASTLYGGTYNLFAHTLPQYGV 129 E RLA +EGG SG AA+ ++GD+++ +YG + +L + + Sbjct: 72 EARLAEIEGGKYCRVTPSGLAAIAMVNFAFLKSGDDVLLPDNVYGPSKDLGQWLERDFQI 131 Query: 130 QTRFADPRQPASFEPLIDDRTKAIYIESIGNPLGNITDIAAVADIAHRHGVPLIVDNTVA 189 RF DP LI T+ ++ E+ G+ + DI A+ AH GV + +DNT + Sbjct: 132 TARFYDPMIGEGLAELIRPTTRLVWTEAPGSVSMEVPDIPALCRAAHAKGVLVAIDNTWS 191 Query: 190 TPYLLRPFEHGADIVVHSLTKYLGGHGTTLGGAIVDSGKFPWAKHKQRFKRLNEPDVSYH 249 + FEHG DIV+ ++TKY G L GA+ Sbjct: 192 AGIAFKAFEHGVDIVMQAVTKYQSGGSDVLMGAV-------------------------- 225 Query: 250 GVVYTEALGEAAYIGRARVVPLRNMGAALSPFNAFQILQGIETLGLRLDRITANTLAVAQ 309 +V +AL E + R +G + + + +L+ + ++ LR + A VA Sbjct: 226 -IVRDQALNEKLELAHMR------LGFGVGMDDVYMVLRSLSSMRLRFEAHDAAARKVAA 278 Query: 310 YLKRHPKVRWVNYAGLEDHPDHALAQTYFKGRASGVLT--FGVANGREGGARFQDALQLF 367 +LK+ P++ V + ED P HA + F G A G+ + F RF D+L+LF Sbjct: 279 WLKQRPEIVAVLHPAFEDCPGHAHWKRDFSG-AGGLFSVIFDPRYVEAQTDRFVDSLRLF 337 Query: 368 TRLVNIGDAKSLATHPASTTHRQLSPTELEKAGVTE-DTVRLSIGIEHIDDLLADLEQAL 426 + G A SL +R + +A + E VR +IG+E +DL+AD+EQAL Sbjct: 338 KIGYSWGGAHSLC-----VPYRM---KGMRRAWMREGQLVRFNIGLESPEDLIADIEQAL 389 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.399 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,353,436 Number of extensions: 57630 Number of successful extensions: 169 Number of sequences better than 1.0e-08: 3 Number of HSP's gapped: 165 Number of HSP's successfully gapped: 3 Length of query: 429 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 341 Effective length of database: 1,203,190 Effective search space: 410287790 Effective search space used: 410287790 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 132 (55.5 bits)