BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0083.p (291 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014... 123 2e-29 gi|300312026|ref|YP_003776118.1| signal transduction protein con... 109 3e-25 gi|300313026|ref|YP_003777118.1| signal transduction protein con... 108 7e-25 gi|300311036|ref|YP_003775128.1| signal transduction protein [He... 107 1e-24 gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [... 105 3e-24 >gi|300309360|ref|YP_003773452.1| hypothetical protein Hsero_0014 [Herbaspirillum seropedicae SmR1] Length = 726 Score = 123 bits (308), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 74/247 (29%), Positives = 126/247 (51%), Gaps = 11/247 (4%) Query: 15 AQLQQALRERQFHPFLQPIVCAAGRWQGAEILMRWQHPQAGLQVPGVFIARLAEQGLLAA 74 A+L+QA+ RQF + QP V G GAE L+RWQHP G+ P FI+ + GL+ Sbjct: 474 AELRQAVARRQFIVYYQPQVTYEGDVVGAEALVRWQHPIRGIVSPTTFISLAEDMGLMDE 533 Query: 75 ATRQMLQQLSM---SWTGAGLALRQPFFLSFNACADQLRGDALVSQCEALLHTMQQASLR 131 RQ+L ++ +W GLA + ++ N A + + D V + ++ S Sbjct: 534 IGRQVLHRICTDLENWQREGLA--RDLVVAINVSAQEFKVDNFVYRFREIMREHHVPSHS 591 Query: 132 LMIEMPERGGDIAPAHCRRLFSQCE---QADIAVALDDFGIGDARLHQLASGRISCLKLD 188 + +E+ E + H + ++ + +++++LDDFG G + L L I +K+D Sbjct: 592 VKVELTE---SMMVDHIEDVITKMQALRSNEVSISLDDFGTGYSSLSYLGRFPIDQIKID 648 Query: 189 RSFVAATPADSLYSRLIEKIVALAAVFNASVTAEGVETLAQWQWLRSIGVRRFQGYYFAR 248 ++FV D+ + +I I+ L N S+ AEGVET Q +L + G +QGY++++ Sbjct: 649 QTFVREMQNDAAMASIIRSIIMLGQSLNLSIIAEGVETEQQRDFLHAQGCLLYQGYWYSK 708 Query: 249 PLAAADF 255 P+ F Sbjct: 709 PIPGEQF 715 >gi|300312026|ref|YP_003776118.1| signal transduction protein containing a membrane domain, an EAL and a GGDEF domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1106 Score = 109 bits (272), Expect = 3e-25, Method: Compositional matrix adjust. Identities = 78/250 (31%), Positives = 119/250 (47%), Gaps = 15/250 (6%) Query: 15 AQLQQALRERQFHPFLQP-IVCAAGRWQGAEILMRWQHPQAGLQVPGVFIARLAEQGLLA 73 A+L++A+ +F QP I A GR GAE L+RWQHP GL PG FI LAE+ L Sbjct: 847 AELRRAIERAEFVVHYQPKIELAEGRIVGAEALVRWQHPLRGLLPPGEFIT-LAEETRLI 905 Query: 74 AATRQMLQQLSMSWTGAGLALRQPFF---LSFNACADQLRGDALVSQCEALLHTMQQASL 130 ++ L ++ GL +Q +S N Q + L+ + ++ Sbjct: 906 DQIGLLV--LDVACRDIGLMRQQQMTCGRISINLTGSQFGDERLLDDIKEVVDRHGTPPS 963 Query: 131 RLMIEMPERGGDIAPAHCRR----LFSQCEQADIAVALDDFGIGDARLHQLASGRISCLK 186 L E+ E H R + +++DDFG G + L L ++ LK Sbjct: 964 CLEFEITES----MVMHNRERAIAIMDGIRAEGFTISIDDFGTGYSSLAYLKRFPVNTLK 1019 Query: 187 LDRSFVAATPADSLYSRLIEKIVALAAVFNASVTAEGVETLAQWQWLRSIGVRRFQGYYF 246 +D+SF+ P + S +++ I+A++ N V EGVET Q Q LR G +QGYYF Sbjct: 1020 IDKSFINDIPDNPNSSAIVQAIIAMSHALNLKVVTEGVETEKQLQALREFGSDEYQGYYF 1079 Query: 247 ARPLAAADFL 256 ++P+ A F+ Sbjct: 1080 SKPVPYAQFV 1089 >gi|300313026|ref|YP_003777118.1| signal transduction protein containing sensor and EAL domains protein [Herbaspirillum seropedicae SmR1] Length = 544 Score = 108 bits (269), Expect = 7e-25, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 122/246 (49%), Gaps = 9/246 (3%) Query: 15 AQLQQALRERQFHPFLQPIV-CAAGRWQGAEILMRWQHPQAGLQVPGVFIARLAEQGLLA 73 A LQ A+++++F QPI+ A+GR GAE L+RW+ P + P FIA E GL+ Sbjct: 271 ADLQLAVKKKEFIVHYQPIIEMASGRCIGAEALLRWRRPDGSIVRPDFFIALAEESGLIL 330 Query: 74 AATRQMLQQLSMSWTGAGLALRQP--FFLSFNACADQLRGDALVSQCEALLHTMQQASLR 131 T ++ + LRQ ++ N A ++ ++ L + Sbjct: 331 PLTDLVVDTVVREMRDT---LRQDRALHIAINVAAQDMKTGRILEVVSQALAGSDIRPEQ 387 Query: 132 LMIEMPERG-GDIAPAHCRRLFSQCEQADIAVALDDFGIGDARLHQLASGRISCLKLDRS 190 L +E ERG DIA A ++ + ++ALDDFG G + L L + LK+D+S Sbjct: 388 LWMEATERGCMDIASASLT--LARAREMGHSMALDDFGTGYSNLQYLQGLPMDALKIDKS 445 Query: 191 FVAATPADSLYSRLIEKIVALAAVFNASVTAEGVETLAQWQWLRSIGVRRFQGYYFARPL 250 FV+ + S +I+ I+A+A + AEGVETL Q +L GV QG+ F+RPL Sbjct: 446 FVSTIGTGAATSAVIDHIIAMAKSLKLYIVAEGVETLEQAAYLAERGVDFAQGWLFSRPL 505 Query: 251 AAADFL 256 +F+ Sbjct: 506 PVEEFV 511 >gi|300311036|ref|YP_003775128.1| signal transduction protein [Herbaspirillum seropedicae SmR1] Length = 750 Score = 107 bits (266), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 86/254 (33%), Positives = 117/254 (46%), Gaps = 21/254 (8%) Query: 16 QLQQALRERQFHPFLQP-IVCAAGRWQGAEILMRWQHPQAGLQVPGVFIARLAEQGLLAA 74 L AL+E QF QP + +G AE L+RW+HP G+ P FI E GL+ Sbjct: 491 DLYDALQEGQFELHFQPALELQSGVIHKAEALVRWRHPAFGMISPDRFIPLAEESGLIFD 550 Query: 75 ATRQMLQQ---LSMSWTGAGLALRQPFF--LSFNACADQLRGDALVSQCEALLHTMQQAS 129 R +LQ+ +S+ W G + PF +S N Q L +A++ M Q S Sbjct: 551 LGRWILQEAARVSVRWNAQGTS---PFRKQISVNLSPVQF----LNGHYDAIV--MDQLS 601 Query: 130 LR------LMIEMPERGGDIAPAHCRRLFSQCEQADIAVALDDFGIGDARLHQLASGRIS 183 L+ + IE+ E R + A I VALDDFG G + + L + Sbjct: 602 LQGCQPGLIAIEITEGLLLDDSEAVRSQLNNLRAAGIQVALDDFGTGYSAMSYLKKFSMD 661 Query: 184 CLKLDRSFVAATPADSLYSRLIEKIVALAAVFNASVTAEGVETLAQWQWLRSIGVRRFQG 243 LK+DRSFV ++E I+ +A V AEGVET+ Q Q LR G QG Sbjct: 662 YLKIDRSFVQDMVDADSNKAIVEAIIVMAHRLGMKVIAEGVETMVQAQLLRDAGCEYVQG 721 Query: 244 YYFARPLAAADFLG 257 Y ++RPL DFL Sbjct: 722 YLYSRPLPEDDFLN 735 >gi|300311871|ref|YP_003775963.1| EAL domain-containing protein [Herbaspirillum seropedicae SmR1] Length = 1036 Score = 105 bits (263), Expect = 3e-24, Method: Compositional matrix adjust. Identities = 86/261 (32%), Positives = 122/261 (46%), Gaps = 14/261 (5%) Query: 4 PTVSTAPPMARAQLQQALRE----RQFHPFLQPIVCAAGRWQGAEILMRWQHPQAGLQVP 59 P + TA + RA L+ LR+ Q QP V GR GAE+L+RWQHPQ G+ P Sbjct: 767 PAMQTAV-LERATLEAGLRKAIDGNQLLLHYQPQVVEGGRVTGAEVLVRWQHPQRGMVPP 825 Query: 60 GVFIARLAEQGLLAAATRQMLQ----QLSMSWTGAGLALRQPFFLSFNACADQLRGDALV 115 G FI E GL+ + +L+ QL+ T A ++ N A QLR V Sbjct: 826 GDFIPLAEETGLILSLGNWVLETACRQLA---TWAAQPQMADLSIAVNVSAPQLREPDFV 882 Query: 116 SQCEALLHTMQQASLRLMIEMPERG-GDIAPAHCRRLFSQCEQADIAVALDDFGIGDARL 174 + RL +E+ E D +++F+ + + +LDDFG G + L Sbjct: 883 QTVLDTIERTGANPRRLKLELTESLLVDNVEDIIQKMFA-LKAYGVEFSLDDFGTGYSSL 941 Query: 175 HQLASGRISCLKLDRSFVAATPADSLYSRLIEKIVALAAVFNASVTAEGVETLAQWQWLR 234 L ++ LK+D+SFV D + + + IV LA V AEGVET AQ +L Sbjct: 942 SYLKRLPLNQLKIDQSFVRDVLIDPNDASIAKTIVNLAQSLGLGVIAEGVETDAQRSFLA 1001 Query: 235 SIGVRRFQGYYFARPLAAADF 255 G +QGY+F RP+ F Sbjct: 1002 DAGCHAYQGYFFCRPVTVEAF 1022 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.134 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 899,546 Number of extensions: 36116 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 18 Number of HSP's gapped: 169 Number of HSP's successfully gapped: 18 Length of query: 291 Length of database: 1,619,870 Length adjustment: 84 Effective length of query: 207 Effective length of database: 1,222,130 Effective search space: 252980910 Effective search space used: 252980910 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 130 (54.7 bits)