BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0077.p (303 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herba... 620 e-179 gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herba... 313 2e-86 gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxy... 135 3e-33 gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase... 63 2e-11 >gi|300312960|ref|YP_003777052.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 303 Score = 620 bits (1600), Expect = e-179, Method: Compositional matrix adjust. Identities = 301/303 (99%), Positives = 303/303 (100%) Query: 1 VDKKAIDAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA 60 +DKKAIDAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA Sbjct: 1 MDKKAIDAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSA 60 Query: 61 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPERKGFARMDK 120 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPE+KGFARMDK Sbjct: 61 GKSGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPEQKGFARMDK 120 Query: 121 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQG 180 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQG Sbjct: 121 DPQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQG 180 Query: 181 RYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPY 240 RYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPY Sbjct: 181 RYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPY 240 Query: 241 LWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPR 300 LWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPR Sbjct: 241 LWDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPR 300 Query: 301 LAA 303 LAA Sbjct: 301 LAA 303 >gi|300310639|ref|YP_003774731.1| catechol 1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 311 Score = 313 bits (801), Expect = 2e-86, Method: Compositional matrix adjust. Identities = 154/286 (53%), Positives = 195/286 (68%), Gaps = 3/286 (1%) Query: 21 GNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGKSGEFGLIAAGLGFEHFLD 80 GN R K +V+R++ D+F TIE+L++ +E+WA V Y+ G + E GL++ GLG + FLD Sbjct: 26 GNPRTKQIVHRILSDLFKTIEDLEITSDEYWAGVAYINRLGTAHEAGLLSPGLGLDRFLD 85 Query: 81 LRMDEAEARRGVQGGTPRTIEGPLYVSGAPERKGFARMDKDP-QPGDTLFMQGQVFDEHG 139 +RMD +A G+ GGTPRTIEGPLYV+GAP +GFAR+D + G T+ M G V+ G Sbjct: 86 MRMDAEDAALGIAGGTPRTIEGPLYVAGAPVEQGFARLDDGSDKSGQTIIMHGTVYGADG 145 Query: 140 KPLANALVEVWHANHLGRYSFFDP--DQSPFNLRCSIRTDEQGRYRFRSRVPVGYSVPPG 197 KP+ A VEVWHAN G YS FDP +Q PFN+R +I TD QGRY+FRS +P Y PP Sbjct: 146 KPMPGAQVEVWHANTKGFYSHFDPTGEQKPFNMRRTIITDAQGRYKFRSIMPTAYGCPPA 205 Query: 198 GATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYLWDDFAFATREGLVPEL 257 G T LL LGRHG+RPAHIHFFVT G+RKLTTQINI GDP ++DDFA+ATREGLVP L Sbjct: 206 GPTQGLLNALGRHGNRPAHIHFFVTAEGHRKLTTQINIEGDPLIYDDFAYATREGLVPPL 265 Query: 258 KHVTDPAEIRKHEVDAPFYAIEFNFNLLPEQADLPKADINRPRLAA 303 TD A I+ ++ PF I F+ L ++RPRLAA Sbjct: 266 VERTDAASIKAEGLEGPFAEIVFDIKLTSLVNGADNQIVDRPRLAA 311 >gi|300313016|ref|YP_003777108.1| 6-chlorohydroxyquinol-1,2-dioxygenase [Herbaspirillum seropedicae SmR1] Length = 296 Score = 135 bits (341), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 87/285 (30%), Positives = 139/285 (48%), Gaps = 11/285 (3%) Query: 7 DAVLQKIESTELAEGNQRVKTVVNRLVRDMFYTIEELDVQPEEFWAAVDYLTSAGK---- 62 D + Q + S +QR++ ++ LV+ + E+ + +E++A +D+LT G+ Sbjct: 15 DTITQAVLSRHAWLTDQRLREIMTSLVQHLHAFAREVKLTEKEWFAGIDFLTETGQICSD 74 Query: 63 -SGEFGLIAAGLGFEHFLDLRMDEAEARRGVQGGTPRTIEGPLYVSGAPERKGFARMDKD 121 EF L++ LG + + + A G T T+ GP +V AP+ A + Sbjct: 75 QRQEFILLSDTLGLSTLVMAQQNHKPA-----GCTEATVFGPFHVEDAPQYPLGADIANG 129 Query: 122 PQPGDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQSPFNLRCSIRTDEQGR 181 + G+ F+ G V +G+PLA+A +EVW A+ G Y D + R ++ TD +GR Sbjct: 130 AK-GEPCFVSGSVRGRNGRPLAHARMEVWQADENGFYDVQYADLDQYRARGTLFTDAEGR 188 Query: 182 YRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGYRKLTTQINIAGDPYL 241 Y FRS + Y +P G R+L KLGRH RPAH+HF + GY L T + D YL Sbjct: 189 YHFRSILANPYPIPHDGPVGRMLEKLGRHPWRPAHLHFRIQAEGYETLITHVFRRDDVYL 248 Query: 242 WDDFAFATREGLVPELKHVTDPAEIRKHEVDAPFYAIEFNFNLLP 286 D F R L+ + + + PFY ++++F L P Sbjct: 249 DSDAVFGVRSTLIADWPCHPAGTAPDGTQCEQPFYTLDYDFVLNP 293 >gi|300313273|ref|YP_003777365.1| protocatechuate 3,4-dioxygenase beta chain protein [Herbaspirillum seropedicae SmR1] Length = 230 Score = 63.2 bits (152), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 48/132 (36%), Positives = 66/132 (50%), Gaps = 19/132 (14%) Query: 125 GDTLFMQGQVFDEHGKPLANALVEVWHANHLGRYSFFDPDQ--SPFNLRCS----IRTDE 178 G+ + + G+V DE GKP+ N+L+EVW N GRY F DQ +P + + + TD+ Sbjct: 68 GEKIVVTGRVVDEDGKPVRNSLLEVWQCNAAGRY-FHKKDQHDAPLDPNFTGFGKMLTDD 126 Query: 179 QGRYRFRSRVPVGYSVPPGGATDRLLTKLGRHGSRPAHIHFFVTVPGY-RKLTTQINIAG 237 GRYRF S P Y P G + RPAHIHF + Y ++L TQ+ Sbjct: 127 NGRYRFVSIKPGPY---PWGNHHK--------AWRPAHIHFSLFGNVYAQRLVTQMYFPN 175 Query: 238 DPYLWDDFAFAT 249 DP D F + Sbjct: 176 DPLFAYDPIFQS 187 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.320 0.139 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,083,295 Number of extensions: 50402 Number of successful extensions: 104 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 98 Number of HSP's successfully gapped: 4 Length of query: 303 Length of database: 1,619,870 Length adjustment: 85 Effective length of query: 218 Effective length of database: 1,217,395 Effective search space: 265392110 Effective search space used: 265392110 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 130 (54.7 bits)