BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0076.p (386 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 775 0.0 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 500 e-143 gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 67 2e-12 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 66 5e-12 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 775 bits (2001), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/386 (99%), Positives = 384/386 (99%) Query: 1 LNPTLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG 60 +NPTLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG Sbjct: 1 MNPTLPATSIETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGG 60 Query: 61 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDAL 120 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDAL Sbjct: 61 LAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDAL 120 Query: 121 ARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQ 180 ARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQ Sbjct: 121 ARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQ 180 Query: 181 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAAGNHDGLR 240 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAA NHDGLR Sbjct: 181 QDCAHVAAIKRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLR 240 Query: 241 RLRQKNRIAIMADESLHGPQDAYQLARVGAADVFAVKINQSGGLAGAQQVAAIASAAQVE 300 RLRQKNRIAIMADESLHGPQDAYQLAR GAADVFAVKINQSGGLAGAQQVAAIASAAQVE Sbjct: 241 RLRQKNRIAIMADESLHGPQDAYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVE 300 Query: 301 LYGGTMLEGAIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGP 360 LYGGTMLEGAIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGP Sbjct: 301 LYGGTMLEGAIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGP 360 Query: 361 GLGITLDEDRVNALRRDRKTPLVSLV 386 GLGITLDEDRVNALRRDRKTPLVSLV Sbjct: 361 GLGITLDEDRVNALRRDRKTPLVSLV 386 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 500 bits (1288), Expect = e-143, Method: Compositional matrix adjust. Identities = 251/370 (67%), Positives = 296/370 (80%), Gaps = 2/370 (0%) Query: 10 IETVEVLLLDVPTIRPHRLSMTTMHRQTLVLVRITGNDGVAGWGEATTIGGLAYGEESPE 69 I+ VE L+D+PTIRPH+LSM TM+ QTL+LVR+ +DG G GE TTIGGLAYG ESPE Sbjct: 16 IDRVETFLVDLPTIRPHQLSMATMNGQTLMLVRLYCSDGSIGVGEGTTIGGLAYGAESPE 75 Query: 70 SIKVNIDTYITPLLLGADANQVAARMHHIDEHVQGNRFAKCAIETALSDALARRLGVPLS 129 +K+ IDTY PLLLGADA+Q+ A M +++ + NRFAKCAIETAL DAL +R G+P+S Sbjct: 76 GMKLAIDTYFAPLLLGADASQIPAIMARLNKAICDNRFAKCAIETALFDALGQRTGLPVS 135 Query: 130 TLFGGRVRDSLPIAWTLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAI 189 L GGR R+SLPIAWTLASGDTA+DIDEAE+MLAQRRHN+FKLKIG R V D AHVAAI Sbjct: 136 QLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRHNIFKLKIGRREVDADVAHVAAI 195 Query: 190 KRALGERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAAGNHDGLRRLRQKNRIA 249 K+ALG+RG+VRVDVN AW+E EA G+ L DAG +LVEQP+A + L RL+ ++ IA Sbjct: 196 KKALGDRGAVRVDVNMAWSELEAQRGLAGLVDAGCELVEQPVA--SPQALARLKGRHAIA 253 Query: 250 IMADESLHGPQDAYQLARVGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEG 309 IMADESL GP A+ LAR+G ADVFA+KI QSGGL AQQVAAI AA +ELYGGTMLEG Sbjct: 254 IMADESLTGPASAFALARIGGADVFAIKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEG 313 Query: 310 AIGTMASAQLFATFGTLAWGSELFGPLLLTEEILATPLRYRDFALELPTGPGLGITLDED 369 AIGT+ASA FATF L WG+ELFGPLLLTEEILA PL+Y+DF L +P PGLGITLDE+ Sbjct: 314 AIGTIASAHAFATFRELQWGTELFGPLLLTEEILAEPLQYQDFQLAIPHRPGLGITLDEE 373 Query: 370 RVNALRRDRK 379 R+ RRDRK Sbjct: 374 RLQYFRRDRK 383 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 67.4 bits (163), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 82/363 (22%), Positives = 149/363 (41%), Gaps = 51/363 (14%) Query: 39 VLVRITGNDGVAGWGEATTIGGLAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHI 98 ++I ++GV GWGE E S+ ++ ++ L+G D + H Sbjct: 16 CFLKIETDEGVVGWGEPIV-------EGRAHSVAAAVEE-LSDYLIGKDPRNIE---DHW 64 Query: 99 DEHVQGNRF--------AKCAIETALSDALARRLGVPLSTLFGGRVRDSLPI-AW----- 144 +G + A I+ AL D + LGVP+ L GG VR+S+ + +W Sbjct: 65 TVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWIGGDR 124 Query: 145 ----------TLASGDTARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAIKRALG 194 +A G TA ++ E++ H+ +L + A+V A++ A+G Sbjct: 125 PADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTL---------ANVQAVREAVG 175 Query: 195 ERGSVRVDVNQAWTETEAMLGMQWLADAGVDLVEQPIAAGNHDGLRRLRQKNRIAIMADE 254 + VD + + A ++ L + +E+P+ + N + L+ + I E Sbjct: 176 PNVGIGVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGE 235 Query: 255 SLHGPQDAYQLARVGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEGAIGTM 314 L+ D ++ G D+ ++ +GG+ +++A +A A V L L G I Sbjct: 236 RLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPL-GPIALA 294 Query: 315 ASAQLFATFGTLAWGSELFGPLLLTEEILATPLR------YRDFALELPTGPGLGITLDE 368 + Q+ A + G L +R Y D +++P GPGLGI ++E Sbjct: 295 SCLQVDAGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINE 354 Query: 369 DRV 371 + V Sbjct: 355 EYV 357 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 65.9 bits (159), Expect = 5e-12, Method: Compositional matrix adjust. Identities = 74/295 (25%), Positives = 126/295 (42%), Gaps = 36/295 (12%) Query: 108 AKCAIETALSDALARRLGVPLSTLFGGRVRDSLPIAWTLASGDTARDID----------- 156 A A E A DA AR L VPL L GG VR +P + L A+ ID Sbjct: 110 AYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAYLFF-KYAQHIDSPYAPDAWGEA 168 Query: 157 --------EAEKMLAQRRHNLFKLKIGARTVQQDCAHVAAIKRALGERGSVRVDVNQAWT 208 +A KM+ + KLK GA + + A + A+++A + +R+D N W+ Sbjct: 169 LSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIKALRKAFPD-APLRIDPNGNWS 227 Query: 209 ETEAMLGMQWLADAGVDLVEQPIAAGNHDGLRRLRQKNRIAIMADESLHGPQDAYQLARV 268 A+ + L D + E P +G+ L ++ + + + + D +L R Sbjct: 228 LQTAIRMAELLGD-DLQYYEDPTPG--LEGMAELHRRTGLPLATNMVV---TDFDELRRS 281 Query: 269 GAADVFAVKI---NQSGGLAGAQQVAAIASAA--QVELYGGTMLEGAIGTMASAQLFATF 323 A D + + + GGL QQ+A + V ++ + L I MA + + A Sbjct: 282 VALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNSHL--GISLMAMSHVAAAV 339 Query: 324 GTLAWGSELFGPLLLTEE--ILATPLRYRDFALELPTGPGLGITLDEDRVNALRR 376 L++ + P +E I L R+ +++ PGLG+ +D+D++ L + Sbjct: 340 ENLSYACDTHYPWQEPDEEVIQGGKLPIRNGCVQITDAPGLGVEVDQDQLRKLHQ 394 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.134 0.389 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,198,402 Number of extensions: 50097 Number of successful extensions: 200 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 199 Number of HSP's successfully gapped: 4 Length of query: 386 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 299 Effective length of database: 1,207,925 Effective search space: 361169575 Effective search space used: 361169575 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)