BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0063.p (650 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite s... 1309 0.0 gi|300311886|ref|YP_003775978.1| multi-sensor signal transductio... 89 8e-19 gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine ... 71 3e-13 gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine ... 70 4e-13 >gi|300312939|ref|YP_003777031.1| transmembrane nitrate/nitrite sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 650 Score = 1309 bits (3387), Expect = 0.0, Method: Compositional matrix adjust. Identities = 648/650 (99%), Positives = 649/650 (99%) Query: 1 MDPAAAPFAPLPPHKRLTFRILLMTLIGLGLTMTAIGYTLLLSWQLEGGGTVINEAGSLR 60 MDPAAAPFAPLPPHKRLTFRILLMTLIGLGLTMTAIGYTLLLSWQLEGGGTVINEAGSLR Sbjct: 1 MDPAAAPFAPLPPHKRLTFRILLMTLIGLGLTMTAIGYTLLLSWQLEGGGTVINEAGSLR 60 Query: 61 MRSYQLGLALEMHQRDSAVLPEIERFDQILRSLHTGQGDQSGQGGQPSFVPPRGPIHEQL 120 MRSYQLGLALEMHQRDSAVLPEIERFDQILRSLHTGQGDQSGQGGQPSFVPPRGPIHEQL Sbjct: 61 MRSYQLGLALEMHQRDSAVLPEIERFDQILRSLHTGQGDQSGQGGQPSFVPPRGPIHEQL 120 Query: 121 LRVQDGWTHRMQVHARAALETADPALRRQAITLYLQELPDFVDDINLLVALVEADLAGKT 180 LRVQDGWTHRMQVHARAALETADP LRRQAITLYLQELPDFVDDINLLVALVEADLAGKT Sbjct: 121 LRVQDGWTHRMQVHARAALETADPVLRRQAITLYLQELPDFVDDINLLVALVEADLAGKT 180 Query: 181 TWLRLCQTALIFLALAASIAVLYLLYLWIVGPVRRMQAGIARMSMNDLDVRLPIESADEF 240 TWLRLCQTALIFLALAASIAVLYLLYLWIVGPVRRMQAGIARMSMNDLDVRLPIESADEF Sbjct: 181 TWLRLCQTALIFLALAASIAVLYLLYLWIVGPVRRMQAGIARMSMNDLDVRLPIESADEF 240 Query: 241 GQLACAFNQMADHVRSVHRTLEQRVAEKTAQLQEQNDEVSTLYEVTGFLSGAHAIEALCG 300 GQLACAFNQMADHVRSVHRTLEQRVAEKTAQLQEQNDEVSTLYEVTGFLSGAHAIEALCG Sbjct: 241 GQLACAFNQMADHVRSVHRTLEQRVAEKTAQLQEQNDEVSTLYEVTGFLSGAHAIEALCG 300 Query: 301 GFLQRIMARLQADGGTVRILDERGHLHLTVHEGVSEAIIEAERCLKVDDCLCGSATQQGV 360 GFLQRIMARLQADGGTVRILDERGHLHLTVHEGVSEAIIEAERCLKVDDCLCGSATQQGV Sbjct: 301 GFLQRIMARLQADGGTVRILDERGHLHLTVHEGVSEAIIEAERCLKVDDCLCGSATQQGV 360 Query: 361 IIVRDFHELGERRRYRCKEEGARSIGIFQILVREQVIGSFSLHFSHPREISADERRLLET 420 IIVRDFHELGERRRYRCKEEGARSIGIFQILVREQVIGSFSLHFSHPREISADERRLLET Sbjct: 361 IIVRDFHELGERRRYRCKEEGARSIGIFQILVREQVIGSFSLHFSHPREISADERRLLET 420 Query: 421 LGRHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDR 480 LGRHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDR Sbjct: 421 LGRHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDR 480 Query: 481 LDEIRDIAPLLRTGVQENYEDVRELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQIT 540 LDEIRDIAPLLRTGVQENYEDVRELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQIT Sbjct: 481 LDEIRDIAPLLRTGVQENYEDVRELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQIT 540 Query: 541 LTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQDFRMTVTDDGHGFTLE 600 LTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQDFRMTV+DDGHGFTLE Sbjct: 541 LTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQDFRMTVSDDGHGFTLE 600 Query: 601 SARAKGLEHVGLRIMQERAERLGANLEIDSQPGHGTRIALTLLRQERLVA 650 SARAKGLEHVGLRIMQERAERLGANLEIDSQPGHGTRIALTLLRQERLVA Sbjct: 601 SARAKGLEHVGLRIMQERAERLGANLEIDSQPGHGTRIALTLLRQERLVA 650 >gi|300311886|ref|YP_003775978.1| multi-sensor signal transduction histidine kinase [Herbaspirillum seropedicae SmR1] Length = 475 Score = 89.4 bits (220), Expect = 8e-19, Method: Compositional matrix adjust. Identities = 68/227 (29%), Positives = 115/227 (50%), Gaps = 15/227 (6%) Query: 423 RHLGSAIENQRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLD 482 R L AI+ Q+L++ ER LA+GLHD + Q L L + ++ ++ + + Sbjct: 248 RDLELAIQRQQLLSSRVIEVQERERQHLARGLHDDVGQSLTALKINLETIKKMGSLE--- 304 Query: 483 EIRDIAPLLRTG---VQENYEDVRELLLNFRT-RLQDSNLDSEMRKALDKLQRQSGLQGQ 538 P L+ G V VR L L+ R +L + L + +R +++ + + + G Sbjct: 305 -----GPSLQNGIDIVTAVLAQVRSLSLDLRPPQLDNLGLIAALRSYVEQKSKLANVNGW 359 Query: 539 ITLTGDGPPLAPEQQLQVLFILQEALSNIRKHARAQQVQISVDNRQD-FRMTVTDDGHGF 597 +G L + + I+QEA++NI +HAR Q + + +D + D +++ DDG GF Sbjct: 360 FESSGHDEQLHHDIENTAFRIVQEAVTNILRHARCQNIWVKIDRQHDQVYLSIRDDGRGF 419 Query: 598 TLESARAKGLE--HVGLRIMQERAERLGANLEIDSQPGHGTRIALTL 642 +E AR+ + GL M+ERA +G ++EI S PG GT I + L Sbjct: 420 DIERARSNAISGTSFGLLNMEERAVLVGGSMEITSAPGAGTEIVMHL 466 >gi|300311282|ref|YP_003775374.1| transmembrane sensor histidine kinase transcription regulator protein [Herbaspirillum seropedicae SmR1] Length = 464 Score = 70.9 bits (172), Expect = 3e-13, Method: Compositional matrix adjust. Identities = 69/228 (30%), Positives = 106/228 (46%), Gaps = 27/228 (11%) Query: 432 QRLIAREKEFAIANERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDIAPLL 491 QR++ ++E ER L++ LHD I+Q L + LQ++ A RL+ + A Sbjct: 240 QRVVRSQEE-----ERARLSRDLHDGISQWLVSIKLQIEA-----ALVRLNGTPEQAATA 289 Query: 492 RTGVQENYEDVRELLLNFR--------TRLQDSNLDSEMRKALDKLQRQSGLQGQITLTG 543 R G ++ + +L R L D L + + + + +G +I Sbjct: 290 RAGFEKTATQINGVLAEVRRISHDLRPAILDDLGLAAALEHLCGEWRDAAGAGVEIVFEN 349 Query: 544 -----DGPPLAPEQQLQVLF-ILQEALSNIRKHARAQQVQISVDNRQDFRM-TVTDDGHG 596 DG L P+ VLF I QEAL+NI +HA A ++++ + + M V D+GHG Sbjct: 350 QAQADDGATL-PDVVNTVLFRIAQEALTNIARHAGASRIKVRLYQGHNLLMLQVQDNGHG 408 Query: 597 FTLESARAKGLEHVGLRIMQERAERLGANLEIDSQPGHGTRIALTLLR 644 F L +GLR M+ER E +G L IDS P GT I+ + R Sbjct: 409 FDLGQVDDHSRHGIGLRNMRERIEAVGGELGIDSSPA-GTSISAVIAR 455 >gi|300310534|ref|YP_003774626.1| transmembrane sensor histidine kinase [Herbaspirillum seropedicae SmR1] Length = 677 Score = 70.5 bits (171), Expect = 4e-13, Method: Compositional matrix adjust. Identities = 59/207 (28%), Positives = 103/207 (49%), Gaps = 8/207 (3%) Query: 446 ERNLLAQGLHDSIAQGLNFLNLQVQMLEDSLARDRLDEIRDI--APLLRTGVQENYEDVR 503 E+ +A+ LHD++ L L++ ++ + L D R + L+++ V Sbjct: 472 EKRAIARELHDNMGASLTALSMHLEGVYQILPPDEKWADRKVRMQGLMKSLVATTRRIQT 531 Query: 504 ELLLNFRTRLQDSNLDSEMRKALDKLQRQSGLQGQITLTGDGPPLAPEQQLQVLFILQEA 563 EL N L L + + + LD+L ++G+ +L + + E ++ + +LQE Sbjct: 532 ELRPN---TLDLFGLKAAISEQLDELHERTGIACHASLPDEDVEVGHEMEIAIYRMLQEM 588 Query: 564 LSNIRKHARAQQVQISVDNRQD-FRMTVTDDGHGFTLESARAKGLEHVGLRIMQERAERL 622 L+N+ KHA+A +V + +D +D +TV DDG G E R + GLR ++ERA Sbjct: 589 LNNVTKHAKASKVDVILDIDEDHVALTVRDDGVGIPEE--RRDNHKTYGLRGLRERATFF 646 Query: 623 GANLEIDSQPGHGTRIALTLLRQERLV 649 G ++I S PG G I ++L + LV Sbjct: 647 GGEVDIRSTPGKGALITISLPIRPELV 673 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.322 0.137 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,798,523 Number of extensions: 72664 Number of successful extensions: 332 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 328 Number of HSP's successfully gapped: 5 Length of query: 650 Length of database: 1,619,870 Length adjustment: 91 Effective length of query: 559 Effective length of database: 1,188,985 Effective search space: 664642615 Effective search space used: 664642615 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 134 (56.2 bits)