BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0055.p (421 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter pro... 838 0.0 gi|300312933|ref|YP_003777025.1| nitrite/nitrate transporter pro... 82 6e-17 gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite t... 79 9e-16 >gi|300312932|ref|YP_003777024.1| nitrite/nitrate transporter protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 838 bits (2166), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/421 (99%), Positives = 420/421 (99%) Query: 1 MSDPRKANGVLASSTLAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI 60 MSDPRKANGVLASSTLAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI Sbjct: 1 MSDPRKANGVLASSTLAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLI 60 Query: 61 RVPLGIWTDRYGGRIVFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPY 120 RVPLGIWTDRYGGRIVFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPY Sbjct: 61 RVPLGIWTDRYGGRIVFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPY 120 Query: 121 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAPVLIASAGWTLVPQVYAGLMLAAALLFWL 180 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAPVLIASAGWTLVPQVYAGLMLAAAL+FWL Sbjct: 121 VARWFKKERQGLAMGVFGAGNSGAAVNKFIAPVLIASAGWTLVPQVYAGLMLAAALIFWL 180 Query: 181 GSATDPSHQVSSDTRFMDQVRMLRDLRVWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD 240 GSATDPSHQVSSDTRFMDQVRMLRD RVWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD Sbjct: 181 GSATDPSHQVSSDTRFMDQVRMLRDPRVWRYCQYYSVVFGGYVGLSLWMVKYYVTEYGFD 240 Query: 241 IRHAALLAACFSLPGGVLRALGGWLSDRFGAYQVTWWVMWVCWVAFFLLSYPQTDFVIQT 300 IRHAALLAACFSLPGGVLRALGGWLSDRFGAYQVTWWVMWVCWVAFFLLSYPQTDFVIQT Sbjct: 241 IRHAALLAACFSLPGGVLRALGGWLSDRFGAYQVTWWVMWVCWVAFFLLSYPQTDFVIQT 300 Query: 301 VGGPQAFHIGLNPVLFTILLFVVGVAMAIGKASVFKFISDDYSSNIGAVSGIVGLAGGLG 360 VGGPQAFHIGLNPVLFTILLFVVGVAMAIGKASVFKFISDDYSSNIGAVSGIVGLAGGLG Sbjct: 301 VGGPQAFHIGLNPVLFTILLFVVGVAMAIGKASVFKFISDDYSSNIGAVSGIVGLAGGLG 360 Query: 361 GFILPILFGLALDLTGVRSSCFMLLYGTVCISLVWMHFQFRREYPQTSSVAPAAPVTSDA 420 GFILPILFGLALDLTGVRSSCFMLLYGTVCISLVWMHFQFRREYPQTSSVAPAAPVTSDA Sbjct: 361 GFILPILFGLALDLTGVRSSCFMLLYGTVCISLVWMHFQFRREYPQTSSVAPAAPVTSDA 420 Query: 421 A 421 A Sbjct: 421 A 421 >gi|300312933|ref|YP_003777025.1| nitrite/nitrate transporter protein [Herbaspirillum seropedicae SmR1] Length = 460 Score = 82.4 bits (202), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 110/434 (25%), Positives = 176/434 (40%), Gaps = 61/434 (14%) Query: 20 VCFTIWMMFGVIGIPIKNA-LHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRIVFF 78 + F +WM++ V+ + A H ++ + LTA+P L+G+ +R+ +GGR Sbjct: 37 LAFAVWMLWSVVAANLDKAGFHFSKDQLFFLTALPALSGATLRIFYSFLVPVFGGRKWTA 96 Query: 79 LLMLSTVIP-----IWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPYVARWFKKERQGLA 133 + S +IP + L + L L L GL GG+FS ++ +F K R+G A Sbjct: 97 ISTASLLIPAIGMGLALQDPTTRYSTLLGLALLCGLGGGNFSSSMANISFFFPKARKGYA 156 Query: 134 MGVF-GAGNSGAAVNKFIAPVLIASA--GWTLVPQ----------------------VYA 168 G+ G GN G +V +F+ P++IASA G + Q ++ Sbjct: 157 TGMNAGIGNLGVSVVQFVTPLVIASAMFGSAVSGQGFLFHDPKSGLESSFWLANAGFIWV 216 Query: 169 GLMLAAALLFWLGSATDPSHQVSSDTRFMDQVRMLRDLRVWRYCQYYSVVFGGYVGLSLW 228 ++AA + W G + S+ DQ + + W C Y FG ++G S Sbjct: 217 PFIIAATVAAWFGM----NDIASAHASLADQAVIFKRKHNWLMCWLYVGTFGSFIGFSAA 272 Query: 229 MVKYYVTEYGFDIRHAALLAACFSLPGGVLRALGGWLSDRFGAYQVTWWVMWVCWVAFFL 288 E F A A L G + R LGG +SDR G +VT+WV + L Sbjct: 273 FAMLIKAE--FPEIAVAQYAFLGPLAGALARPLGGMISDRVGGARVTFWVFLAMGLIILL 330 Query: 289 LSYPQTD--------------FVIQTVGGPQAFHIGLNPVLFTILLFVVGVAMAIGKASV 334 P F++ +G F + PV+F A+ + Sbjct: 331 GILPALHTHQFMLFLASFIGMFILTGLGNGSTFC--MIPVIFQT---ERERAVQGQGEAA 385 Query: 335 FKFISDDYSSNIGAVSGIVGLAGGLGGFILPILFGLALDLTGVRSSC---FMLLYGTVCI 391 + D AV G G G GGF +P FG ++ +TG F+ Y + C+ Sbjct: 386 QQQARLDAGKESAAVLGFSGAIGAYGGFFIPQSFGTSIKMTGAPDMAMYVFIAFYAS-CM 444 Query: 392 SLVWMHFQFRREYP 405 ++ W ++ RR P Sbjct: 445 AITWWYYA-RRNAP 457 >gi|300312204|ref|YP_003776296.1| high affinity nitrate/nitrite transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 435 Score = 78.6 bits (192), Expect = 9e-16, Method: Compositional matrix adjust. Identities = 77/278 (27%), Positives = 117/278 (42%), Gaps = 28/278 (10%) Query: 16 LAFTVCFTIWMMFGVIGIPIKNALHLNETEFGLLTAMPVLTGSLIRVPLGIWTDRYGGRI 75 +AF VCF W + IK HL + + V L+R+ +G DR+G R Sbjct: 25 MAFFVCFFAWFACAPLMPVIKGEFHLTPGQIANINIAAVAVTILVRLIIGPMCDRFGPRK 84 Query: 76 VFFLLMLSTVIPIWLISYAHAFWHFLVLGLFVGLAGGSFSVGTPYVARWFKKERQGLA-M 134 + L+L+ +P+ +++A ++ FL L +G G SF + + + F G A Sbjct: 85 TYTGLLLAGAVPVIGVAFAQSYESFLFFRLLIGAVGASFVITQYHTSVMFAPNVVGTANA 144 Query: 135 GVFGAGNSGAAVNKFIAPVLIA-------SAGW-----TLVPQVYAGLMLAAALLFWLGS 182 G GN+G V + + P+L+A S GW LVP V LML A L+W + Sbjct: 145 ATAGWGNAGGGVAQGVMPLLMAALLMLGVSQGWGWRAALLVPGV---LMLVMAALYWRFT 201 Query: 183 ATDPSHQ----------VSSDTR--FMDQVRMLRDLRVWRYCQYYSVVFGGYVGLSLWMV 230 P + S R + + RVW Y FG + + Sbjct: 202 QDCPEGNYDALRAAGVTIESGKRGGWASFRAAAANYRVWMLFITYGACFGVEIFIHNIAA 261 Query: 231 KYYVTEYGFDIRHAALLAACFSLPGGVLRALGGWLSDR 268 YYV + + A + AA F L RALGG++SD+ Sbjct: 262 IYYVEHFKLSLESAGIAAASFGLLALFARALGGFISDK 299 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.329 0.143 0.468 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,314,247 Number of extensions: 55354 Number of successful extensions: 337 Number of sequences better than 1.0e-08: 8 Number of HSP's gapped: 331 Number of HSP's successfully gapped: 8 Length of query: 421 Length of database: 1,619,870 Length adjustment: 88 Effective length of query: 333 Effective length of database: 1,203,190 Effective search space: 400662270 Effective search space used: 400662270 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 132 (55.5 bits)