BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0050.p (824 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrog... 1651 0.0 gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbasp... 127 3e-30 gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbasp... 100 7e-22 gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [H... 89 2e-18 >gi|300312928|ref|YP_003777020.1| transmembrane acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 824 Score = 1651 bits (4276), Expect = 0.0, Method: Compositional matrix adjust. Identities = 815/824 (98%), Positives = 816/824 (99%) Query: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL Sbjct: 1 MIIWLILLIASALALMMMRASAWAWLAAEAVLLWLGARLAEIEWPVLVLLAILFFTPTLL 60 Query: 61 VAIRSVRQALMTRPALDTFRKIMPPMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 VAIRSVRQALMTRPALD FRKIMP MSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA Sbjct: 61 VAIRSVRQALMTRPALDIFRKIMPAMSATERDAIEAGTVWWDAELFSGKPDWSRLFALPA 120 Query: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ Sbjct: 121 PRLTAEEQAFLDNEVEQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQ 180 Query: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGREIPC 240 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQG EIPC Sbjct: 181 FSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGLEIPC 240 Query: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD Sbjct: 241 FALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASD 300 Query: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA Sbjct: 301 PDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKDVFIPMEWVIGGVA 360 Query: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL Sbjct: 361 QVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEAL 420 Query: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHVTERGRMVVNDGMDVIGGKGICM 480 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYH+TERGRMVVNDGMDVIGGKGICM Sbjct: 421 ARMGGHLYMMDATRRLSALAVDAGEKPSVISAISKYHITERGRMVVNDGMDVIGGKGICM 480 Query: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ 540 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ Sbjct: 481 GPGNFLARVYQQIPIAITVEGANILTRCLIIFGQGAIRCHPYVLKELAAAADEDRERAVQ 540 Query: 541 EFDRLLFGHLSFVAANAARSLVGGLSGGWLLPALWQAAHATRRYYRAVVHLSSAFALLTD 600 EFDRLLFGHLSFVAANAARSLVGGLSGGWLL A QAAHATRRYYRAVVHLSSAFALLTD Sbjct: 541 EFDRLLFGHLSFVAANAARSLVGGLSGGWLLAAPAQAAHATRRYYRAVVHLSSAFALLTD 600 Query: 601 ASMAVLGGTLKFRERISARLGDVLSQLYLVSAVLKRYEDEGRQEADLPYVDWSVQEALNR 660 ASMAVLGGTLKFRERISARLGDVLSQLYLVSA LKRYEDEGRQEADLPYVDWSVQEALNR Sbjct: 601 ASMAVLGGTLKFRERISARLGDVLSQLYLVSAALKRYEDEGRQEADLPYVDWSVQEALNR 660 Query: 661 AQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHVAKAMQTPGESRERLLAD 720 AQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHVAKAMQTPGESRERLLAD Sbjct: 661 AQEAIVEVLHNFPNPFVAFALRLAIFPLGLPYRKPADVLGTHVAKAMQTPGESRERLLAD 720 Query: 721 AYLPRAGDPLACGEQAFALTPVVQQLEHRLKPDIREGKLPPMPQSLLELTAWSALALERG 780 AYLPRAGDPLACGEQAFALTPVVQQLEHRLKPDIREGKLPPMPQSLLELTAWSALALERG Sbjct: 721 AYLPRAGDPLACGEQAFALTPVVQQLEHRLKPDIREGKLPPMPQSLLELTAWSALALERG 780 Query: 781 LLSQQEKEALDAFALYGHQSVAVDDFAADFDRAEMVAQVAAQSS 824 LLS QEKEALDAFALYGHQSVAVDDFAADFDRAEMVAQVAAQSS Sbjct: 781 LLSLQEKEALDAFALYGHQSVAVDDFAADFDRAEMVAQVAAQSS 824 >gi|300310260|ref|YP_003774352.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 127 bits (320), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 109/380 (28%), Positives = 176/380 (46%), Gaps = 33/380 (8%) Query: 136 EQLCGMVSDWDATVKTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQFSAYMHSQVVMKLAS 195 E+L +DWD + P EA + + + G +GM +P Q+GG + ++A+ Sbjct: 20 ERLLPFAADWD---RNHTFPAEALKELAELGAMGMCVPEQWGGAGMDYMSLVLALEEIAA 76 Query: 196 RSSAAAIAVMVPNSLGPAELLLHYGTEVQKNHYLPRLAQGREIPCFALTSPYAGSDAAAI 255 A + V V NSL + YG+ QK +L LA+G + CF LT P+ GSDAAAI Sbjct: 77 GDGATSTIVSVQNSLACG-ITEKYGSPTQKEQWLKPLARGEMLGCFCLTEPHTGSDAAAI 135 Query: 256 PDIGIVCKGVHEGRETLGFRVTWSKRYITLAPIATVLGLAFRASDPDGLLGGEREAGITC 315 R+ F + +K++IT A V + F +D G++ GI+C Sbjct: 136 R--------TRAERDGDDFILNGTKQFITSGKHAGV-AIVFAVTDRS---AGKK--GISC 181 Query: 316 ALIPTDHPGVVTGRRHWPLNAVFQNGPTEGKD-VFIPMEWVIGGVAQVGRGWRMLMECLA 374 L+P D PG V GR + + D +P ++G + G G+R+ + L Sbjct: 182 FLVPCDTPGFVVGRSEEKMGQHASDTVQIMLDNCRVPATALLG---KEGEGYRIALSNLE 238 Query: 375 AGRAISLPSSSVGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEALARMGGHLYMMDATR 434 AGR I + + SVG ++ A YA R F + I + + V LA M ++DA R Sbjct: 239 AGR-IGIAAQSVGMARAAFEAAVSYARQRESFGVPIIEHQAVNFRLADMN---TLLDAAR 294 Query: 435 RL---SALAVDAGEKPSVISAISKYHVTERGRMVVNDGMDVIGGKGICMGPGNF-LARVY 490 + +A D G ++++K +E + +D + + GG G +F + R+Y Sbjct: 295 LMVWRAAQLKDQGRPCLKEASMAKMFASEAAEKIASDAIQIHGGVGY---TSDFPVERIY 351 Query: 491 QQIPIAITVEGANILTRCLI 510 + + I EGAN + R +I Sbjct: 352 RDVRICQIYEGANDIQRLVI 371 >gi|300311865|ref|YP_003775957.1| acyl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 99.8 bits (247), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 97/350 (27%), Positives = 166/350 (47%), Gaps = 31/350 (8%) Query: 166 GFLGMIIPRQYGGKQFSAYMHSQVVMKLASRSSAAAIAVMVPNSLGPAELLLHYGTEVQK 225 GF G+ P GG S + V +LA ++ A + + N + + ++ +G K Sbjct: 49 GFCGLYTPEADGGLGLSRLDATVVFEELARACTSTAAYLTIHNMV--SWMIASWGQPSLK 106 Query: 226 NHYLPRLAQGREIPCFALTSPYAGSDAAAIPDIGIVCKGVHEGRETLGFRVTWSKRYITL 285 + +LA GR+I + LT P +GSDAA++ + G H + + SK +I+ Sbjct: 107 AQWCEQLAAGRKIGSYCLTEPGSGSDAASLKTTARLEDG-H-------YVLNGSKAFISG 158 Query: 286 APIATVLGLAFRASDPDGLLGGEREAGITCALIPTDHPGVVTGRRHWPLNAVFQNGPTEG 345 A VL + R GGE G++ ++P + PGV G++ + + + PT Sbjct: 159 AGSTDVLVVMART-------GGEGARGVSAIVVPGNAPGVSYGKKESKMG--WNSQPTRT 209 Query: 346 ---KDVFIPMEWVIGGVAQVGRGWRMLMECLAAGRAISLPSSSVGFSKMAVRGTSGYAAM 402 +V +P + ++G Q G+G+ M+ L GR I++ + SVG ++ A+ Y Sbjct: 210 ISFDNVKVPADHLLG---QEGQGFIFAMKGLDGGR-INIATCSVGAAQAALDAAHAYMKE 265 Query: 403 RRQFKIEIGKFEGVQEALARMGGHLYMMDATRRLSALAVDAGE-KPSVISAISKYHVTER 461 R+QF I F+ +Q LA M L RL+A +DAG + + A++K T+ Sbjct: 266 RKQFGRPIADFQALQFKLADMQTELVAARQMVRLAATKLDAGSPEATTYCAMAKRLATDL 325 Query: 462 GRMVVNDGMDVIGGKG-ICMGPGNFLARVYQQIPIAITVEGANILTRCLI 510 G V N+ + + GG G I P L R ++ + + +EG N + R +I Sbjct: 326 GFRVCNEALQLHGGYGYIREYP---LERYFRDVRVHQILEGTNEIMRVII 372 >gi|300313969|ref|YP_003778061.1| isovaleryl-CoA dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 394 Score = 88.6 bits (218), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 90/338 (26%), Positives = 147/338 (43%), Gaps = 32/338 (9%) Query: 150 KTQDLPPEAWQFIKDKGFLGMIIPRQYGGKQFSAYMHSQVVMKLASRSSAAAIAVMVPNS 209 ++ P + W+ + D G LG+ + +YGG Y+ + M+ SR+SA+ +S Sbjct: 39 RSDQFPMDLWKKLGDLGVLGITVSEEYGGAGL-GYLAHIIAMEEISRASASVGLSYGAHS 97 Query: 210 LGPAELLLHYGTEVQKNHYLPRLAQGREIPCFALTSPYAGSDAAAIPDIGIVCKGVHEGR 269 + G E QK YLPRL G I A++ P AGSD +V + + Sbjct: 98 NLCVNQIKRNGNEEQKRKYLPRLISGDFIGALAMSEPNAGSD--------VVSMKLRADK 149 Query: 270 ETLGFRVTWSKRYITLAPIATVLGLAFRASDPDGLLGGEREAGITCALIPTDHPGVVTGR 329 + + + SK +IT P A VL + + +D L G R G+T L+ + G + Sbjct: 150 KGDRYVLNGSKMWITNGPDADVL-VVYAKTD---LEAGAR--GMTAFLVEKGYKGFSVAQ 203 Query: 330 RHWPLNAVFQNGPTEGKDVF----IPMEWVIGGVAQVGRGWRMLMECLAAGRAISLPSSS 385 + L+ + G G+ VF +P E V+GG VGRG +LM L R++ L Sbjct: 204 K---LDKLGMRGSHTGELVFQDCEVPEENVLGG---VGRGVNVLMSGLDFERSV-LSGGP 256 Query: 386 VGFSKMAVRGTSGYAAMRRQFKIEIGKFEGVQEALARMGGHLYMMDATRRLSALAVDAGE 445 +G + + Y R+QF IG+F+ +Q LA M + A A D + Sbjct: 257 LGIMQACMDVVVPYVHDRKQFGQAIGEFQLMQGKLADMYSTMMACKAYVYAVGQACDRAD 316 Query: 446 KPSVISAISK------YHVTERGRMVVNDGMDVIGGKG 477 + A+ K + E+ + + + +GG G Sbjct: 317 SADKVRALRKDAAGAILYSAEKATWMAGEAIQSLGGNG 354 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.323 0.136 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,449,752 Number of extensions: 107427 Number of successful extensions: 322 Number of sequences better than 1.0e-08: 4 Number of HSP's gapped: 317 Number of HSP's successfully gapped: 4 Length of query: 824 Length of database: 1,619,870 Length adjustment: 93 Effective length of query: 731 Effective length of database: 1,179,515 Effective search space: 862225465 Effective search space used: 862225465 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 135 (56.6 bits)