BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0017.p (939 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312898|ref|YP_003776990.1| translation initiation factor I... 1601 0.0 gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor p... 92 2e-19 gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor p... 65 2e-11 gi|300309454|ref|YP_003773546.1| GTPase translation elongation f... 64 7e-11 gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein... 64 7e-11 >gi|300312898|ref|YP_003776990.1| translation initiation factor IF-2 [Herbaspirillum seropedicae SmR1] Length = 954 Score = 1601 bits (4145), Expect = 0.0, Method: Compositional matrix adjust. Identities = 926/940 (98%), Positives = 927/940 (98%), Gaps = 1/940 (0%) Query: 1 MPADLLLTQLRSAGVEKSSASDSLSKEDKDRLLEHLRRSRGAAPEGEKKKITLTRKETTE 60 MPADLLLTQLRSAGVEKSSASDSLSKEDKDRLLEHLRRSRGAAPEGEKKKITLTRKETTE Sbjct: 15 MPADLLLTQLRSAGVEKSSASDSLSKEDKDRLLEHLRRSRGAAPEGEKKKITLTRKETTE 74 Query: 61 IKQADATGKSRTIQVEVRKKRTFIKRDEPGVEETAARAAAPQRDPAIEAQEREQEEARRQ 120 IKQADATGKSRTIQVEVRKKRTFIKRDEPGVEETAARAAAPQRDPAIEAQEREQEEARRQ Sbjct: 75 IKQADATGKSRTIQVEVRKKRTFIKRDEPGVEETAARAAAPQRDPAIEAQEREQEEARRQ 134 Query: 121 AELKAQQERLAQLEAERAAQAKAAELEQQRAREEAEAEAKRQAAEAAAAAEAEKAKAAAP 180 AELKAQQERLAQLEAERAAQAKAAELEQQRAREEAEAEAKRQAAEAAAAA + AA Sbjct: 135 AELKAQQERLAQLEAERAAQAKAAELEQQRAREEAEAEAKRQAAEAAAAAAEAEKAKAAA 194 Query: 181 AAAAAAPAPA-VDPAAEEKKRAAAEEAKKKAEAAAKEAADRAAAADRARKAVEDEVAQIK 239 AAAAA VDPAAEEKKRAAAEEAKKKAEAAAKEAADRAAAADRARKAVEDEVAQIK Sbjct: 195 PAAAAAAPAPAVDPAAEEKKRAAAEEAKKKAEAAAKEAADRAAAADRARKAVEDEVAQIK 254 Query: 240 AMMNAPRRVIKAPEPAPAAAKTSEGTLHKPATAAKPGEKKDDKKPAVAADKKSIKSANVS 299 AMMNAPRRVIKAPEPAPAAAKTSEGTLHKPATAAKPGEKKDDKKPAVAADKKSIKSANVS Sbjct: 255 AMMNAPRRVIKAPEPAPAAAKTSEGTLHKPATAAKPGEKKDDKKPAVAADKKSIKSANVS 314 Query: 300 STWQDDAKKRGTGGMKSRGATGGGGRDGWRAGPKGRRHSHGDDQRESNFQVPTEAVVHDV 359 STWQDDAKKRGTGGMKSRGATGGGGRDGWRAGPKGRRHSHGDDQRESNFQVPTEAVVHDV Sbjct: 315 STWQDDAKKRGTGGMKSRGATGGGGRDGWRAGPKGRRHSHGDDQRESNFQVPTEAVVHDV 374 Query: 360 YVPETITVAEVAHKMAVKASEVIKHLMKLGQMVTINQVLDQETAMIVVEEMGHRAHAAKL 419 YVPETITVAEVAHKMAVKASEVIKHLMKLGQMVTINQVLDQETAMIVVEEMGHRAHAAKL Sbjct: 375 YVPETITVAEVAHKMAVKASEVIKHLMKLGQMVTINQVLDQETAMIVVEEMGHRAHAAKL 434 Query: 420 DDPEALLVEGEEHGDAEALPRAPVVTVMGHVDHGKTSLLDYIRRTKVASGEAGGITQHIG 479 DDPEALLVEGEEHGDAEALPRAPVVTVMGHVDHGKTSLLDYIRRTKVASGEAGGITQHIG Sbjct: 435 DDPEALLVEGEEHGDAEALPRAPVVTVMGHVDHGKTSLLDYIRRTKVASGEAGGITQHIG 494 Query: 480 AYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAG 539 AYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAG Sbjct: 495 AYHVETPRGMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAG 554 Query: 540 GVPLVVAINKIDKPGANPDRVKQELIAEEVVPEEYGGDAPFIPVSAKTGEGIDSLLENVL 599 GVPLVVAINKIDKPGANPDRVKQELIAEEVVPEEYGGDAPFIPVSAKTGEGIDSLLENVL Sbjct: 555 GVPLVVAINKIDKPGANPDRVKQELIAEEVVPEEYGGDAPFIPVSAKTGEGIDSLLENVL 614 Query: 600 LQAEVLELKAPVEVPARGLVIEAKLDKGRGPVATILVQSGTLKRGDVVLAGSAYGRVRAM 659 LQAEVLELKAPVEVPARGLVIEAKLDKGRGPVATILVQSGTLKRGDVVLAGSAYGRVRAM Sbjct: 615 LQAEVLELKAPVEVPARGLVIEAKLDKGRGPVATILVQSGTLKRGDVVLAGSAYGRVRAM 674 Query: 660 LDENGKNIAEAGPSLPVEIQGLTEVPSAGEEVVVMTDERKAREIGLFRQGKFRDVKLAKQ 719 LDENGKNIAEAGPSLPVEIQGLTEVPSAGEEVVVMTDERKAREIGLFRQGKFRDVKLAKQ Sbjct: 675 LDENGKNIAEAGPSLPVEIQGLTEVPSAGEEVVVMTDERKAREIGLFRQGKFRDVKLAKQ 734 Query: 720 QAAKLENMFDQMTEGEVKNLPMIIKTDVQGSQEALVQSLQKLSTNEVRVQVVHAAVGGIS 779 QAAKLENMFDQMTEGEVKNLPMIIKTDVQGSQEALVQSLQKLSTNEVRVQVVHAAVGGIS Sbjct: 735 QAAKLENMFDQMTEGEVKNLPMIIKTDVQGSQEALVQSLQKLSTNEVRVQVVHAAVGGIS 794 Query: 780 ENDVNLAVASKAVIIGFNTRADASARKLAEASGVDIRYYNIIYDAVDEVKAAMSGMLSPE 839 ENDVNLAVASKAVIIGFNTRADASARKLAEASGVDIRYYNIIYDAVDEVKAAMSGMLSPE Sbjct: 795 ENDVNLAVASKAVIIGFNTRADASARKLAEASGVDIRYYNIIYDAVDEVKAAMSGMLSPE 854 Query: 840 KREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVVVWTGELDSLKRFK 899 KREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVVVWTGELDSLKRFK Sbjct: 855 KREQALGLVEIRQVFVVSKVGSIAGCYVLEGLVKRGSQVRLLRNNVVVWTGELDSLKRFK 914 Query: 900 DDVKEVKSGFECGLSLKGYNDIEVGDQLEIFEVQEVARTL 939 DDVKEVKSGFECGLSLKGYNDIEVGDQLEIFEVQEVARTL Sbjct: 915 DDVKEVKSGFECGLSLKGYNDIEVGDQLEIFEVQEVARTL 954 >gi|300311258|ref|YP_003775350.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 597 Score = 91.7 bits (226), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 73/232 (31%), Positives = 113/232 (48%), Gaps = 41/232 (17%) Query: 444 VTVMGHVDHGKTSLLDYIRRTKVASGEAGGIT------QHIGAYHVETPRGM-------- 489 +++ H+DHGK++L D I + GG++ Q + + +E RG+ Sbjct: 7 FSIIAHIDHGKSTLADRIIQL------CGGLSNREMEAQVLDSMDIERERGITIKAQTAA 60 Query: 490 ------------ITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAK 537 + +DTPGH F+ +R A + +LVV A GV QT A Sbjct: 61 LSYKAKDGQIYNLNLIDTPGHVDFSYEVSRSLSACEGALLVVDASQGVEAQTVANCYTAL 120 Query: 538 AGGVPLVVAINKIDKPGANPDRVKQELIAEEVVPEEYGGDAP-FIPVSAKTGEGIDSLLE 596 GV +V +NKID P A+PD K E+ E+V+ G DA P SAKTG G++ +LE Sbjct: 121 DLGVEVVPVLNKIDLPSADPDNAKSEI--EDVI----GIDASDATPCSAKTGLGVEDVLE 174 Query: 597 NVLLQAEVLELKAPVEVPARGLVIEAKLDKGRGPVATILVQSGTLKRGDVVL 648 ++ A+V K E P + L+I++ D G V + + +GTL+ D +L Sbjct: 175 AIV--AKVPAPKGDPEAPLQALIIDSWFDNYVGVVMLVRIVNGTLRPKDKIL 224 >gi|300312895|ref|YP_003776987.1| GTP-binding elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 627 Score = 65.5 bits (158), Expect = 2e-11, Method: Compositional matrix adjust. Identities = 42/123 (34%), Positives = 60/123 (48%), Gaps = 16/123 (13%) Query: 449 HVDHGKTSLLDYIRRTKVASGEAGGITQHI-GAYHVETPRGM---------------ITF 492 HVDHGKT+L+D + R + + + + +E RG+ I Sbjct: 30 HVDHGKTTLVDQLLRQSGTFRDNQQVDNRVMDSNDIEKERGITILSKNCAVEYKGTHINI 89 Query: 493 LDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVPLVVAINKIDK 552 +DTPGH F R D V+L+V A +G MPQT+ A A G+ +V +NKID+ Sbjct: 90 VDTPGHADFGGEVERVLSMVDSVLLLVDAQEGPMPQTRFVTRKALALGLKPIVVLNKIDR 149 Query: 553 PGA 555 PGA Sbjct: 150 PGA 152 >gi|300309454|ref|YP_003773546.1| GTPase translation elongation factor TU [Herbaspirillum seropedicae SmR1] Length = 396 Score = 63.5 bits (153), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 77/232 (33%), Positives = 102/232 (43%), Gaps = 33/232 (14%) Query: 444 VTVMGHVDHGKTSLLDYIRR--TKVASGEAG--------------GITQHIGAYHVETPR 487 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 Query: 488 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 546 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 Query: 547 INKIDKPGANPDRVKQELIAEEV--VPEEY---GGDAPFIPVSAKTG-EGIDSLL--ENV 598 +NK D D EL+ EV + +Y G D P + SAK EG L + + Sbjct: 135 LNKAD---MVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALEGDTGPLGEQAI 191 Query: 599 LLQAEVLELKAPV-EVPARGLVIEAKLD----KGRGPVATILVQSGTLKRGD 645 + AE L+ P E G + D GRG V T V+ G +K G+ Sbjct: 192 MALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGE 243 >gi|300309440|ref|YP_003773532.1| EF-Tu elongation factor protein [Herbaspirillum seropedicae SmR1] Length = 396 Score = 63.5 bits (153), Expect = 7e-11, Method: Compositional matrix adjust. Identities = 77/232 (33%), Positives = 102/232 (43%), Gaps = 33/232 (14%) Query: 444 VTVMGHVDHGKTSLLDYIRR--TKVASGEAG--------------GITQHIGAYHVETPR 487 V +GHVDHGKT+L I +K GEA GIT + ET Sbjct: 15 VGTIGHVDHGKTTLTAAIATVLSKKFGGEAKAYDQIDAAPEEKARGITINTAHVEYETAN 74 Query: 488 GMITFLDTPGHEAFTAMRARGAKATDIVILVVAADDGVMPQTKEAIAHAKAGGVP-LVVA 546 +D PGH + GA D ILV +A DG MPQT+E I ++ GVP ++V Sbjct: 75 RHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLSRQVGVPYIIVF 134 Query: 547 INKIDKPGANPDRVKQELIAEEV--VPEEY---GGDAPFIPVSAKTG-EGIDSLL--ENV 598 +NK D D EL+ EV + +Y G D P + SAK EG L + + Sbjct: 135 LNKAD---MVDDAELLELVEMEVRELLSKYEFPGDDLPIVKGSAKLALEGDTGPLGEQAI 191 Query: 599 LLQAEVLELKAPV-EVPARGLVIEAKLD----KGRGPVATILVQSGTLKRGD 645 + AE L+ P E G + D GRG V T V+ G +K G+ Sbjct: 192 MALAEALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRVERGIIKVGE 243 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.311 0.127 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,211,064 Number of extensions: 88939 Number of successful extensions: 303 Number of sequences better than 1.0e-08: 6 Number of HSP's gapped: 296 Number of HSP's successfully gapped: 6 Length of query: 939 Length of database: 1,619,870 Length adjustment: 94 Effective length of query: 845 Effective length of database: 1,174,780 Effective search space: 992689100 Effective search space used: 992689100 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 135 (56.6 bits)