BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0021.0003.p (400 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembr... 137 2e-33 gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herb... 107 2e-24 gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux ... 99 5e-22 gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herb... 89 4e-19 gi|300312340|ref|YP_003776432.1| transporter transmembrane prote... 63 4e-11 >gi|300312245|ref|YP_003776337.1| transmembrane efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 395 Score = 137 bits (344), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 127/368 (34%), Positives = 177/368 (48%), Gaps = 6/368 (1%) Query: 20 ALLALTMTSFIATANESVPAGLLSGIAHGLGVSEAGAGQLVTCCAFGSGVAAIPLTALFA 79 AL ALT+++F E V GLL +A LGVS + AG LVT A G V A LTA Sbjct: 4 ALWALTISAFAIGTTEFVIMGLLPEVAANLGVSLSSAGLLVTGYALGVFVGAPLLTATTG 63 Query: 80 EWARRRLLVVALSIFLIGNAMTAATDSYLAALFARLVIGLATGVTWSLLAGYARALVEPE 139 R+ LLV + +F++GN A Y + R+V A G + + + A +LV E Sbjct: 64 RLPRKTLLVALMVVFVVGNLACAIAPDYTTLMIGRVVASFAHGAFFGVGSVVATSLVPKE 123 Query: 140 YQGRAMAVAMAGIPLALALGMPLGSWLGAVSGWRSVFLFLAAMAAALIVWVYAAVPELQG 199 Q A+A+ G+ +A LG+P G+WLG GWR+ F + + + VP QG Sbjct: 124 KQASAIALMFTGLTVANVLGVPFGTWLGQSYGWRATFWAVTVIGVIALAATAVLVPRSQG 183 Query: 200 SRTLRRRRLPEVLATPGVRPVLVVLALWIFSHYLMYTYVSPMLAWLGFEP--YLQQVLLI 257 + VL P V L++ L + +TY++P+L L P + +LL+ Sbjct: 184 GEETDFGKEVRVLMRPQVMLGLLMTVLGFGGVFAAFTYIAPILTDLSGFPASAVSPILLL 243 Query: 258 FGLSAVVGNWLVGMLIDRWLRRLVLACLAAFAATAAGFAWSPSNPVLASVGIVIWGL-SF 316 FGL V+GN G L D+ L +L L AA F + N V A +G+ + G+ +F Sbjct: 244 FGLGLVIGNLAGGKLADKKLMATLLGSLVLLAAVLVLFGLTVDNKVAAIIGVGLMGMAAF 303 Query: 317 GGAPTLLQTALADQAGADANIAQSVLVTVFNLVFAGSGAFGGALLDM-RGAGALPCVAAG 375 P L L GA N+A S + FNL A GG +D G A+P VAA Sbjct: 304 ATVPPLQMRVLEKATGAP-NLASSANIAAFNLGNAAGAWLGGVTIDHGPGLHAVPYVAA- 361 Query: 376 LLATAGIV 383 LL AGIV Sbjct: 362 LLPLAGIV 369 >gi|300310405|ref|YP_003774497.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 422 Score = 107 bits (266), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 105/346 (30%), Positives = 170/346 (49%), Gaps = 2/346 (0%) Query: 23 ALTMTSFIATANESVPAGLLSGIAHGLGVSEAGAGQLVTCCAFGSGVAAIPLTALFAEWA 82 +L + F E +PA LL+ IA L VS+ AGQ VT A VAA + L Sbjct: 41 SLALGVFSLVTAEFLPASLLTAIASDLHVSDGAAGQAVTATAIVGAVAAPSIPLLTRNID 100 Query: 83 RRRLLVVALSIFLIGNAMTAATDSYLAALFARLVIGLATGVTWSLLAGYARALVEPEYQG 142 R+R++++ + L+ N + + L AR+++G+A G WS+ A A LV Sbjct: 101 RKRVVLMLSLLLLVSNILAVTAPNLPVLLVARVLLGVALGGFWSMAAALAMRLVPDHLFA 160 Query: 143 RAMAVAMAGIPLALALGMPLGSWLGAVSGWRSVFLFLAAMAAALIVWVYAAVPELQGSRT 202 RAM++ + G+ +A P+G+++G + GWRS F+ ++ I + A+P L Sbjct: 161 RAMSLILTGVSVATVCAAPIGAYMGNLWGWRSAFVAAGVVSLVAIAIQWIAIPRLPPKDH 220 Query: 203 LRRRRLPEVLATPGVRPVLVVLALWIFSHYLMYTYVSPMLAWLGFEPY--LQQVLLIFGL 260 + L E+L VR L+ + L + H+ +TY+ P++ + + VLL +G+ Sbjct: 221 PSLKVLAELLGRKDVRVALLAVLLVVSGHFAGFTYIRPLMEQITHMSVTTISAVLLGYGV 280 Query: 261 SAVVGNWLVGMLIDRWLRRLVLACLAAFAATAAGFAWSPSNPVLASVGIVIWGLSFGGAP 320 GN+ G + +R R +++ A A AA + S+PV+A+V I +WG +FG P Sbjct: 281 GGFFGNFAGGFIAERSERYAIVSGGALIALLAASLWVAGSSPVVAAVAITLWGFAFGAFP 340 Query: 321 TLLQTALADQAGADANIAQSVLVTVFNLVFAGSGAFGGALLDMRGA 366 QT + A A A +LV F + A GG L+D GA Sbjct: 341 VGFQTWIVRAAPDHAEGAGGLLVAAFQVAIASGAIGGGVLVDHVGA 386 >gi|300313915|ref|YP_003778007.1| transmembrane arabinose efflux transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 403 Score = 99.4 bits (246), Expect = 5e-22, Method: Compositional matrix adjust. Identities = 99/310 (31%), Positives = 155/310 (50%), Gaps = 22/310 (7%) Query: 75 TALFAEWARRRLLVVALSIFLIGNAMTAATDSYLAALFARLVIGLATGVTWSLLAGYARA 134 T L A RRR+L+ A++ F N + A + S+ L ARL++ +A G+ + A A Sbjct: 80 TVLSAAMNRRRVLIAAMTAFTAANLLAACSSSFTGLLVARLLMAVAAGLYTPNASALAGA 139 Query: 135 LVEPEYQGRAMAVAMAGIPLALALGMPLGSWLGAVSGWRSVFLFLAAMAAALIVWVYAAV 194 +V P +GRA+A+ G+ ALG+PLG+ +G GWR+ FL ++AM A + + V Sbjct: 140 MVAPGQRGRALAIVSGGMTSPSALGLPLGAVVGHAFGWRATFLAVSAMGAVALAGILLGV 199 Query: 195 PELQGSRT----LRRRRLPEVLATPGVRPVLVVLALWIFSHYLMYTYVSPMLAW-LGF-E 248 P G+ LR R V+ P V+ +L V LW Y Y Y++P L+ L F E Sbjct: 200 PRQAGAHLHVAGLRERM--AVIGQPQVQRLLAVTLLWSVGAYAAYPYIAPYLSHVLSFGE 257 Query: 249 PYLQQVLLIFGLSAVVGNWLVGMLIDRW-----LRRLVLACLAAFAATAAGFAWSPS--- 300 + + ++G +A VG + G L DR+ +R +L + +F AA A P Sbjct: 258 SRISLTVSLWGAAAAVGVSVGGTLNDRYGSARVVRVSLLLLMLSFWTLAAATALPPPLAL 317 Query: 301 NPVLASVGIVIWGLS-FGGAPTLLQTALADQAGADANIAQSVLVTVFNLVFAGSGAFGGA 359 PVL + +V+WG S + P + + A A A +A S+ + L F+ A G Sbjct: 318 GPVL--LAVVVWGFSVWAFFPAQMARLIGAGAPAQAPVALSLNTSTLYLGFSLGSAAGAG 375 Query: 360 LLDMRGAGAL 369 +L G+GA+ Sbjct: 376 IL---GSGAI 382 >gi|300312509|ref|YP_003776601.1| arabinose efflux permease [Herbaspirillum seropedicae SmR1] Length = 385 Score = 89.4 bits (220), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 102/342 (29%), Positives = 164/342 (47%), Gaps = 26/342 (7%) Query: 21 LLALTMTSFIATANESVPAGLLSGIAHGLGVSEAGAGQLVTCCAFGSGVAAIPLTALFAE 80 L AL + +F E V GLL +A LGV+ AG LV+ A G A L L A+ Sbjct: 5 LFALAVAAFGIGTTEFVIMGLLPDVARDLGVTAPAAGMLVSGYALGVAAGAPILAILTAK 64 Query: 81 WARRRLLVVALSIFLIGNAMTAATDSYLAALFARLVIGLATGVTWSLLAGYARALVEPEY 140 W R++ LV + +F++GN + A +Y+ + AR+V + + + A LV P+ Sbjct: 65 WPRKQALVGLMCLFILGNVLCALAPNYVFLMVARVVTAFCHAAFFGIGSVVAAELVAPQK 124 Query: 141 QGRAMAVAMAGIPLALALGMPLGSWLGAVSGWRSVFLFLAAMA--AALIVWVY--AAVPE 196 + +A+A+ G+ +A LG+PLG+ G +GWRS F + A+ AA+++W +P Sbjct: 125 RAQAVALMFTGLTVANILGVPLGTAFGHAAGWRSTFGAVTAIGVLAAIMLWTMLPKKIPM 184 Query: 197 LQGSRTLRRRRLPEVLATPGVRPVLVVLALWIFSHYLMYTYVSPMLAWL-GFEPYLQQ-V 254 G+ R + + V+ L+ L S + + TY++ +L + GF + V Sbjct: 185 QAGNIFQEFRSIIDT----RVQLSLLTSTLASISMFSVITYIAYILLDVTGFTTQQESWV 240 Query: 255 LLIFGLSAVVGNWLVGMLIDRWLRRL---VLACLAA----FAATAAGFAWSPSNPVLASV 307 LL+FG VG + G L D L + V C+A F+ T+ + W P LA+ Sbjct: 241 LLLFGAGITVGGLVGGKLADWKLMPVMCGVFLCIAVVLGLFSITSH-YKW----PTLAT- 294 Query: 308 GIVIWG-LSFGGAPTLLQTALADQAGADANIAQSVLVTVFNL 348 + WG +F P Q + D A N+A ++ FNL Sbjct: 295 -MFAWGAFAFAVVPG-AQIRVLDTARGAPNLASTLNQGAFNL 334 >gi|300312340|ref|YP_003776432.1| transporter transmembrane protein [Herbaspirillum seropedicae SmR1] Length = 421 Score = 63.2 bits (152), Expect = 4e-11, Method: Compositional matrix adjust. Identities = 65/235 (27%), Positives = 107/235 (45%), Gaps = 9/235 (3%) Query: 49 LGVSEAGAGQLVTCCAFGSGVAAIPLTALFAE-WARRRLLVVALSIFLIGNAMTAATDSY 107 + ++ G +V+ AF +G + + LTA FA+ + R+RLL+ F++G S+ Sbjct: 52 MQITPQQFGLVVSAYAFSAGASGL-LTAGFADRFDRKRLLLFFYGGFVLGTVWCGLAQSF 110 Query: 108 LAALFARLVIGLATGVTWSLLAGYARALVEPEYQGRAMAVAMAGIPLALALGMPLGSWLG 167 + L AR+V GL GV S+ A L P +GR M V + LG+P+G +L Sbjct: 111 ESLLLARIVTGLFGGVIGSITLAIATDLFAPHLRGRVMGVIQTSFAASQVLGIPIGLYLS 170 Query: 168 AVSGWRSVFLFLAAMAAALIVWVYAAVPELQGSRTLRRR-----RLPEVLATPGVRPVLV 222 W FL +AA+ A + V + + G TL++ L LA P Sbjct: 171 NRWNWHVPFLAMAALGLAGGLVVLVRMQPVDGHLTLKQEHSAWMHLYHTLAEPRYLTAFA 230 Query: 223 VLALWIFSHYLMYTYVSPMLA-WLGFE-PYLQQVLLIFGLSAVVGNWLVGMLIDR 275 AL + +++ + S L LG + +L + L+ GL + L+G D+ Sbjct: 231 TTALLMTGGFMLMPFSSAYLVGNLGIDMAHLPTIYLVTGLCTIAFGPLIGRAADK 285 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.326 0.137 0.426 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,222,790 Number of extensions: 49257 Number of successful extensions: 420 Number of sequences better than 1.0e-08: 7 Number of HSP's gapped: 415 Number of HSP's successfully gapped: 7 Length of query: 400 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 313 Effective length of database: 1,207,925 Effective search space: 378080525 Effective search space used: 378080525 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 132 (55.5 bits)