BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0534.p (382 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbas... 785 0.0 gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lac... 75 1e-14 gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cyc... 73 3e-14 gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbas... 58 2e-09 gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046... 56 5e-09 >gi|300310362|ref|YP_003774454.1| galactonate dehydratase [Herbaspirillum seropedicae SmR1] Length = 382 Score = 785 bits (2026), Expect = 0.0, Method: Compositional matrix adjust. Identities = 382/382 (100%), Positives = 382/382 (100%) Query: 1 MKITKITTFIVPPRWCFLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNI 60 MKITKITTFIVPPRWCFLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNI Sbjct: 1 MKITKITTFIVPPRWCFLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNI 60 Query: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWI 120 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWI Sbjct: 61 EDHWTVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSWI 120 Query: 121 GGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 GGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI Sbjct: 121 GGDRPADTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGI 180 Query: 181 GVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSR 240 GVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSR Sbjct: 181 GVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSR 240 Query: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD Sbjct: 241 WDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVD 300 Query: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR Sbjct: 301 AGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQR 360 Query: 361 AEVGHRWRNPIWRHKDGSFAEW 382 AEVGHRWRNPIWRHKDGSFAEW Sbjct: 361 AEVGHRWRNPIWRHKDGSFAEW 382 >gi|300310475|ref|YP_003774567.1| mandelate racemase/muconate lactonizing [Herbaspirillum seropedicae SmR1] Length = 424 Score = 74.7 bits (182), Expect = 1e-14, Method: Compositional matrix adjust. Identities = 96/400 (24%), Positives = 174/400 (43%), Gaps = 57/400 (14%) Query: 17 FLKIETDEGVVGWGEPIVEGRAHSVAAAVEELSDYLIGKDPRNIEDHWTVLYRG------ 70 ++IETD G +G GE + A + V+E L+G DP N+ + + RG Sbjct: 33 IIEIETDNGYIGLGESYGDAPALKILDLVKE---RLVGLDPFNL-NGLRAIVRGVVAAMA 88 Query: 71 -----------GFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRVYSW 119 G + A+ +A + + A D + + L VP+ LLGGAVR I ++ Sbjct: 89 PASNAGAELAPGSHASKAVS-NAYSAFEVACLDAQARYLNVPLVDLLGGAVRKEIPFSAY 147 Query: 120 IGG------DRPA-----DTAAAAKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQ 168 + D P A + + VA+ ++ NG + ++ E +A ++ Sbjct: 148 LFFKYAQHIDSPYAPDAWGEALSEEQIVAQARKMIEENGFQSIKLKAGALAPEHEVACIK 207 Query: 169 AVREAVGPNVGIGVDFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTS 228 A+R+A P+ + +D +G A + EL L + E+P + E + E+ T Sbjct: 208 ALRKAF-PDAPLRIDPNGNWSLQTAIRM-AELLGDDLQYYEDP--TPGLEGMAELHRRTG 263 Query: 229 TPIALGERLYSRWDFKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCP- 287 P+A + + +R ++ V I+ D + GG+ +T+++A M + + + +++H Sbjct: 264 LPLATNMVVTDFDELRRSVALDSVQIVLADHHYWGGLRDTQQLAKMCDVFGLGVSMHSNS 323 Query: 288 -LGPIALASCLQVDAGSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGP 346 LG I+L + V A N + HY + I+ +G V+I P Sbjct: 324 HLG-ISLMAMSHVAAAVENL---SYACDTHYPWQEPDEEVIQGGK-LPIRNGCVQITDAP 378 Query: 347 GLGIEINEEY------------VRQRAEVGH-RWRNPIWR 373 GLG+E++++ +RQR +VG R P W+ Sbjct: 379 GLGVEVDQDQLRKLHQLYLECGIRQRDDVGQMRKYQPDWK 418 >gi|300312959|ref|YP_003777051.1| muconate and chloromuconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 386 Score = 73.2 bits (178), Expect = 3e-14, Method: Compositional matrix adjust. Identities = 83/356 (23%), Positives = 145/356 (40%), Gaps = 37/356 (10%) Query: 16 CFLKIETDEGVVGWGEPIV-------EGRAHSVAAAVEE-LSDYLIGKDPRNIE---DHW 64 ++I ++GV GWGE E S+ ++ ++ L+G D + H Sbjct: 39 VLVRITGNDGVAGWGEATTIGGLAYGEESPESIKVNIDTYITPLLLGADANQVAARMHHI 98 Query: 65 TVLYRGGFYRGGAIHMSALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRV-YSWIGGD 123 +G + A I+ AL D + LGVP+ L GG VR+S+ + ++ GD Sbjct: 99 DEHVQGNRF--------AKCAIETALSDALARRLGVPLSTLFGGRVRDSLPIAWTLASGD 150 Query: 124 RPADTAAAAKSAVARGFTAVKMN-GTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGV 182 D A K R K+ G +Q +H V A++ A+G + V Sbjct: 151 TARDIDEAEKMLAQRRHNLFKLKIGARTVQQDCAH---------VAAIKRALGERGSVRV 201 Query: 183 DFHGRVHKPMAKALIKELEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLYSRWD 242 D + + A ++ L + +E+P+ + N + L+ + I E L+ D Sbjct: 202 DVNQAWTETEAMLGMQWLADAGVDLVEQPIAASNHDGLRRLRQKNRIAIMADESLHGPQD 261 Query: 243 FKRILSEGYVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPL-GPIALASCLQVDA 301 ++ G D+ ++ +GG+ +++A +A A V L L G I + Q+ A Sbjct: 262 AYQLARAGAADVFAVKINQSGGLAGAQQVAAIASAAQVELYGGTMLEGAIGTMASAQLFA 321 Query: 302 GSYNAFIQEQSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYV 357 + G L +R Y D +++P GPGLGI ++E+ V Sbjct: 322 TFGTLAWGSELFGPLLLTEEILATPLR------YRDFALELPTGPGLGITLDEDRV 371 >gi|300310638|ref|YP_003774730.1| muconate cycloisomerase [Herbaspirillum seropedicae SmR1] Length = 398 Score = 57.8 bits (138), Expect = 2e-09, Method: Compositional matrix adjust. Identities = 74/286 (25%), Positives = 122/286 (42%), Gaps = 32/286 (11%) Query: 82 ALAGIDQALWDIKGKDLGVPVHQLLGGAVRNSIRV-YSWIGGDRPADTAAAAKSAVARGF 140 A I+ AL+D G+ G+PV QLLGG R S+ + ++ GD D A + R Sbjct: 114 AKCAIETALFDALGQRTGLPVSQLLGGRSRESLPIAWTLASGDTAKDIDEAERMLAQRRH 173 Query: 141 TAVKMN-GTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVDFHGRVHKPMAKALIKE 199 K+ G E ++ +A+V A+++A+G + VD + + A+ + Sbjct: 174 NIFKLKIGRRE---------VDADVAHVAAIKKALGDRGAVRVDVNMAWSELEAQRGLAG 224 Query: 200 LEPYKLMFIEEPVLSENAEALKEIAHLTSTPIALGERLY---SRWDFKRILSEGYVDIIQ 256 L +E+PV S A A + H + I E L S + RI G D+ Sbjct: 225 LVDAGCELVEQPVASPQALARLKGRH--AIAIMADESLTGPASAFALARI---GGADVFA 279 Query: 257 PDVSHAGGITETRKIAMMAEAYDVAL----ALHCPLGPIALASCLQVDAGSYNAFIQEQS 312 + +GG+ +++A + +A + L L +G IA A A +E Sbjct: 280 IKIEQSGGLRAAQQVAAIGDAAGIELYGGTMLEGAIGTIASAHAF--------ATFRELQ 331 Query: 313 LGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVR 358 G L + I + Y+D + IP PGLGI ++EE ++ Sbjct: 332 WGTELFGPLLLTEEILAEP-LQYQDFQLAIPHRPGLGITLDEERLQ 376 >gi|300310379|ref|YP_003774471.1| hypothetical protein Hsero_1046 [Herbaspirillum seropedicae SmR1] Length = 425 Score = 55.8 bits (133), Expect = 5e-09, Method: Compositional matrix adjust. Identities = 54/229 (23%), Positives = 97/229 (42%), Gaps = 14/229 (6%) Query: 132 AKSAVARGFTAVKMNGTEELQFIDSHDKIELTLANVQAVREAVGPNVGIGVDFHGRVHKP 191 + A+ +GF VK+ +L + DK +T+A RE +GP + +D + Sbjct: 205 CQEAIDQGFNHVKLKVGRDL----ADDKRRVTIA-----REVLGPERKLMIDANQVWEVH 255 Query: 192 MAKALIKELEPYKLMFIEEPVLSENAEALKEIAH-LTSTPIALGERLYSRWDFKRILSEG 250 A + +L + FIEEP ++ E ++I + + +A GE +R FK+ + Sbjct: 256 QAIDWVNQLAFAQPWFIEEPTSPDDVEGHRKIREGIGAVKVATGEMCQNRVLFKQFIMRD 315 Query: 251 YVDIIQPDVSHAGGITETRKIAMMAEAYDVALALHCPLGPIALASCLQVDAGSYNAFIQE 310 +D++Q D GG+ E + +MA Y + H G + L +Q + I Sbjct: 316 AIDVVQIDSCRLGGVNEILAVMLMAAKYGKVVCPHA--GGVGLCEYVQHLSMIDYLCISG 373 Query: 311 QSLGIHYNESNDLLDYIRDKNVFAYEDGYVKIPQGPGLGIEINEEYVRQ 359 G + L ++ D V ++ P PG IE+ + + Q Sbjct: 374 SKEGRVTEYVDHLHEHFVDPCVV--KNAAYMPPSRPGFSIEMKPQSLEQ 420 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.138 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 1,249,073 Number of extensions: 54428 Number of successful extensions: 146 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 145 Number of HSP's successfully gapped: 5 Length of query: 382 Length of database: 1,619,870 Length adjustment: 87 Effective length of query: 295 Effective length of database: 1,207,925 Effective search space: 356337875 Effective search space used: 356337875 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 131 (55.1 bits)