BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0522.p (585 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosp... 1206 0.0 gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosp... 792 0.0 gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosp... 419 e-118 gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosp... 231 2e-61 gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase ... 186 4e-48 >gi|300310373|ref|YP_003774465.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 585 Score = 1206 bits (3119), Expect = 0.0, Method: Compositional matrix adjust. Identities = 585/585 (100%), Positives = 585/585 (100%) Query: 1 MSSDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPC 60 MSSDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPC Sbjct: 1 MSSDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPC 60 Query: 61 NAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPID 120 NAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPID Sbjct: 61 NAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPID 120 Query: 121 AVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAG 180 AVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAG Sbjct: 121 AVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAG 180 Query: 181 EITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 EITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL Sbjct: 181 EITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 Query: 241 SGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 SGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW Sbjct: 241 SGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 Query: 301 TRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNV 360 TRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNV Sbjct: 301 TRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNV 360 Query: 361 KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDF 420 KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDF Sbjct: 361 KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDF 420 Query: 421 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR 480 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR Sbjct: 421 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR 480 Query: 481 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKV 540 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKV Sbjct: 481 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKV 540 Query: 541 LAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585 LAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH Sbjct: 541 LAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585 >gi|300312632|ref|YP_003776724.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 578 Score = 792 bits (2046), Expect = 0.0, Method: Compositional matrix adjust. Identities = 391/585 (66%), Positives = 467/585 (79%), Gaps = 7/585 (1%) Query: 1 MSSDKKDKSRTLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPC 60 M+ D K +R RS WFG D++GF+YRSW+KN+GIP +F G+PVIGICNT+SELTPC Sbjct: 1 MTDDTKKSTR--RSQAWFGRQDRDGFIYRSWVKNRGIPHDQFDGRPVIGICNTYSELTPC 58 Query: 61 NAHFRKIAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPID 120 N+HFR +AE V+ GI EAGGFP+EFPV S GE+ LRPTAML RNLASMDVEESIR NP+D Sbjct: 59 NSHFRALAEQVKIGIWEAGGFPLEFPVMSLGETLLRPTAMLYRNLASMDVEESIRANPLD 118 Query: 121 AVVLLTGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAG 180 VVLL GCDKTTPALLMGAAS DVP I V+GGPML+GK++GR++GSGT VWQ+SE V+AG Sbjct: 119 GVVLLMGCDKTTPALLMGAASVDVPTIGVSGGPMLSGKYRGRELGSGTGVWQMSEDVRAG 178 Query: 181 EITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 ++T +F AE+ M RS G C TMGTASTMA M E+LGV LPHNAAIPAVDARR VLA Sbjct: 179 KMTQEEFFEAESCMHRSHGHCMTMGTASTMASMVEALGVGLPHNAAIPAVDARRNVLARN 238 Query: 241 SGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDW 300 +G RIV MV E L LSKILTR+AFENAIR NAAIGGSTNAVIHL AIAGRIG+ L+L+DW Sbjct: 239 AGRRIVQMVEEDLVLSKILTRQAFENAIRVNAAIGGSTNAVIHLLAIAGRIGIKLDLKDW 298 Query: 301 TRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNV 360 IG+ P ++DLQPSG++LME+FYYAGGLPAV+R+L + + K ALT NG+T+W+N Sbjct: 299 DDIGQQLPCLLDLQPSGKFLMEDFYYAGGLPAVIRQL---ESVIDKTALTANGKTLWENC 355 Query: 361 KDAPIYNDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDF 420 +DAP +N+EV+R KP E GI IL+GNLAP GAV+KPSAATP L+KHRGRAVVFE+ Sbjct: 356 QDAPNWNEEVIRSFDKPFKEAAGIAILKGNLAPDGAVIKPSAATPALLKHRGRAVVFENS 415 Query: 421 NHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDAR 480 + +RI+D +LDVD +CVLV+KN GPKGYPGMAE GNM LPPK+L G+ DMVR+SDAR Sbjct: 416 DDLHKRIDDENLDVDETCVLVLKNCGPKGYPGMAEAGNMPLPPKILRKGITDMVRVSDAR 475 Query: 481 MSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKV 540 MSGTAYGTV+LHV+PEAAAGGPL +VQ+GDFIELD A KL LD+S+EE+ RR K Sbjct: 476 MSGTAYGTVVLHVSPEAAAGGPLAVVQNGDFIELDVEARKLHLDVSDEELARRRAQWQK- 534 Query: 541 LAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAAVPKHSH 585 E P+M G+ LYVD V QA++G D DFLVG G VPK +H Sbjct: 535 -PELPPQMQRGWVKLYVDHVQQANQGADLDFLVGKSGPYVPKDNH 578 >gi|300313780|ref|YP_003777872.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 594 Score = 419 bits (1077), Expect = e-118, Method: Compositional matrix adjust. Identities = 244/594 (41%), Positives = 352/594 (59%), Gaps = 26/594 (4%) Query: 10 RTLRSAGWFGTADKNGF--MYRSWMKNQGIPDHEFQ-GKPVIGICNTWSELTPCNAHFRK 66 R RS WF D +Y N GI E + G+P+IGI + S+++PCN + Sbjct: 9 RRFRSQDWFDNPDHIDMTALYLERFMNYGITAEELRSGRPIIGIAQSGSDISPCNRIHLE 68 Query: 67 IAEHVRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMDVEESIRGNPIDAVVLLT 126 +A+ VR GI +AGG P+EFP+ E+ RPTA + RNLA + + E + G PIDAVVL T Sbjct: 69 LAKRVRDGIRDAGGIPMEFPLHPIFENCRRPTAAIDRNLAYLGLVEILHGYPIDAVVLTT 128 Query: 127 GCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQLSEQVKAGEITIHD 186 GCDKTTP+ +M AA+ D+PAIV++GGPML+G G +GSG+ +W+ + + AG I Sbjct: 129 GCDKTTPSQIMAAATVDIPAIVLSGGPMLDGWMDGELVGSGSAIWKGRKLLSAGSIDNEK 188 Query: 187 FMAAEAGMSRSAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHLSGMRIV 246 F+ A + S+G CNTMGTASTM MAE+LG+SL +AIPA R +A+ +G RIV Sbjct: 189 FLEIAAASAPSSGHCNTMGTASTMNAMAEALGMSLTGCSAIPAPYRERGQMAYETGRRIV 248 Query: 247 DMVWEGLTLSKILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGVDLELEDWTRIGRG 306 M +E L S ILTR AF +AI NAAIGGSTNA H+ A+A GV+L+ EDW + G Sbjct: 249 GMAYEDLRPSAILTRDAFLDAIVVNAAIGGSTNAQPHIMAMARHAGVELQSEDWMKYGYD 308 Query: 307 TPTIVDLQPSGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVNGQTMWDNVKDAPIY 366 P ++++QP+G+YL E F+ AGG+PA++ L +A L + +T G+TM +N++ Sbjct: 309 VPLLLNMQPAGKYLGERFHRAGGVPAIMWELQQAGKL-RAERITATGKTMAENLQGRASN 367 Query: 367 NDEVVRPLAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHR------------GRA 414 + E++ P A PL E G +L+GNL A++K S + E + R RA Sbjct: 368 DREMIYPFAAPLRERAGFLVLKGNLF-DFAIMKTSVIS-ETFRERYLSTPGQENIFECRA 425 Query: 415 VVFEDFNHYKERINDPDLDVDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMV 474 VVF+ + Y RINDP L +D + +L ++ GP G+PG AEV NM P ++ GV + Sbjct: 426 VVFDGSDDYHARINDPALKIDENTLLAIRGAGPVGWPGSAEVVNMQPPDALIKRGVSTLP 485 Query: 475 RISDARMSGTAYGTVILHVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRL 534 + D R SGT+ IL+ +PE+A GG L ++DGD + +D G+ + +SEEE+ RR Sbjct: 486 TLGDGRQSGTSDSPSILNASPESAVGGGLAYLRDGDRVRIDLNTGECNMLVSEEELARRK 545 Query: 535 -EARAKVLAERKPEMVGGYQSLYVDRVLQADEGCDFDFLVGCRGAA--VPKHSH 585 E V + P +Q +Y V Q + G + + +G A +P+H+H Sbjct: 546 SEGIPPVPPSQTP-----WQEIYRSTVGQLETGACMELALKYQGVAQTLPRHNH 594 >gi|300309867|ref|YP_003773959.1| dihydroxyacid dehydratase/phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 620 Score = 231 bits (588), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 187/611 (30%), Positives = 299/611 (48%), Gaps = 72/611 (11%) Query: 11 TLRSAGWFGTADKNGFMYRSWMKNQGIPDHEFQGKPVIGICNTWSELTPCNAHFRKIAEH 70 T RS + T +N R+ + G+ D +F KP+I + N++++ P + H + + + Sbjct: 3 TYRS--YTTTQGRNMAGARALWRATGMKDGDFT-KPIIAVVNSFTQFVPGHVHLKDLGQL 59 Query: 71 VRRGIIEAGGFPVEFPVFSNGESNLRPTAMLTRNLASMD-----VEESIRGNPIDAVVLL 125 V R I AGG EF + +S A + +L S D VE + + DA+V + Sbjct: 60 VAREIEAAGGVAKEFNTIAVDDSGTHGDARMLYSLPSRDLIADSVEYMVNAHCADAMVCI 119 Query: 126 TGCDKTTPALLMGAASCDVPAIVVTGGPMLNGKHQGRDIGSGTVVWQL---SEQVKAGEI 182 + CDK TP +LM A ++P + V+GGPM GK + + + V +L ++AG+ Sbjct: 120 SNCDKITPGMLMAAMRLNIPVVFVSGGPMEAGKVI-KTVNTDKKVIKLDLVDAMIQAGDK 178 Query: 183 TIHDFMAAEAGMSR--SAGTCNTMGTASTMACMAESLGVSLPHNAAIPAVDARRYVLAHL 240 I D AE S + G+C+ M TA++M C+ E LG+SLP N I A A R L Sbjct: 179 NISDADVAEVERSACPTCGSCSGMFTANSMNCLTEVLGLSLPGNGTIVATHADRKELFLR 238 Query: 241 SGMRIVDMV---WEGLTLS----KILTRKAFENAIRTNAAIGGSTNAVIHLKAIAGRIGV 293 +G IV++ +E S I T++ +ENA+ + ++GGSTN V+HL A A V Sbjct: 239 AGRLIVELAKRHYEQDDYSILPRNICTKETWENAMTLDVSMGGSTNTVLHLLAAAHEAKV 298 Query: 294 DLELEDWTRIGRGTPTIVDLQP-SGRYLMEEFYYAGGLPAVLRRLGEADLLPHKDALTVN 352 + + D RI R P + + P + +Y +E+ + AGG+ ++L L A LL TV+ Sbjct: 299 EFTMADIDRISRRVPCLCKVAPMTNKYHIEDVHRAGGIISILGELARAGLLDTSRP-TVH 357 Query: 353 GQTMWDNVK--DAPIYNDEVVRPLAK---------------------------------- 376 +T+ + ++ D + +D V L + Sbjct: 358 SKTLGEAIEKFDIRVSSDPAVHKLFRAAPGGVPTQVAFSQEERFEANDLDRANGCIRDRE 417 Query: 377 -PLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLDVD 435 +DGG+ +L GN+A +G ++K + ++K G A VFE + E I V Sbjct: 418 HAYSQDGGLAVLYGNIAEKGCIVKTAGVDESILKFSGTARVFESQDAAVEGILGD--KVK 475 Query: 436 ASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLAT-GV-KDMVRISDARMSGTAYGTVILHV 493 A V++++ GPKG PGM E M P + + G+ K +D R SG + G VI H Sbjct: 476 AGDVVIVRYEGPKGGPGMQE---MLYPTSYIKSKGLGKACALFTDGRFSGGSSGLVIGHA 532 Query: 494 APEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEM-KRRLEARAKVLAERKPEMVGGY 552 +PEAA GG +G+V++GD I++D + L IS +++ KRR A+ A +P G Sbjct: 533 SPEAAEGGAIGLVEEGDMIDIDIPNRTINLRISSDDLAKRRTAMEARGDAAWQP----GN 588 Query: 553 QSLYVDRVLQA 563 + V + LQA Sbjct: 589 RQRVVSQALQA 599 >gi|300310433|ref|YP_003774525.1| 6-phosphogluconate dehydratase [Herbaspirillum seropedicae SmR1] Length = 623 Score = 186 bits (473), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 165/555 (29%), Positives = 260/555 (46%), Gaps = 50/555 (9%) Query: 46 PVIGICNTWSELTPCNAHFRKIAEHVRRGIIEAG-------GFPVEFPVFSNGESNLRPT 98 P + I + ++++ + F + + ++ EAG G P + G++ + Sbjct: 68 PSLAIVSAYNDMLSAHQPFERFPQIIKDAAREAGAVAQFAGGSPAMCDGVTQGQAGME-L 126 Query: 99 AMLTRNLASMDVEESIRGNPIDAVVLLTGCDKTTPALLMGAASC-DVPAIVVTGGPMLNG 157 ++ +R+ +M ++ N DA + L CDK P LL+GA +PA+ V GPM +G Sbjct: 127 SLFSRDAIAMATAIALSHNMFDAALYLGVCDKIVPGLLIGALHFGHLPAVFVPAGPMTSG 186 Query: 158 KHQGRDIGSGTVVWQLSEQVKAGEITIHDFMAAEAGMSRSAGTCNTMGTASTMACMAESL 217 + ++ + AG+I + + AE+ AGTC GTA++ + E++ Sbjct: 187 MSNSDKV-------KIRQLYTAGKIGRAELLEAESKSYHGAGTCTFYGTANSNQMLMEAM 239 Query: 218 GVSLPHNAAI-PAVDAR--RYVLAHLSGMRIVDMVWEGLTLSKILTRKAFENAIRTNAAI 274 G+ LP A I P R A +I D+ + + +++ K+ NAI A Sbjct: 240 GLHLPGAAFITPNTPLRDALTAAAAKQAAKITDLGTQYTPVGRMIDEKSIVNAIVALHAT 299 Query: 275 GGSTNAVIHLKAIAGRIGVDLELEDWTRIGRGTPTIVDLQPSGRYLMEEFYYAGGLPAVL 334 GGSTN +HL AIA G+ ++ +D+ ++ P I + P+G + F+ AGG VL Sbjct: 300 GGSTNHTLHLVAIAKAAGIVIDWDDFDKLSAVVPLITKIYPNGTADVNHFHAAGGTGFVL 359 Query: 335 RRLGEADLLPHKDALTVNGQTMWDNVK-------------------DAPIYN-DE-VVRP 373 R L +A L+ H D T+ GQ + + K DAP + DE V+ Sbjct: 360 RELIDAGLM-HDDVTTILGQGLRQHCKEPMLAAEGENGRPGIVTWHDAPAQSGDEGVLGT 418 Query: 374 LAKPLIEDGGICILRGNLAPRGAVLKPSAATPELMKHRGRAVVFEDFNHYKERINDPDLD 433 +AKP DGG+ +L+GNL AV+K SA PE AVVF + LD Sbjct: 419 VAKPFAADGGLKLLQGNLGR--AVIKISAVKPEHRVVEAPAVVFHSQEAFMAAFKAGQLD 476 Query: 434 VDASCVLVMKNVGPKGYPGMAEVGNMGLPPKVLATGVKDMVRISDARMSGTAYGTV--IL 491 D V+ + GP+ GM E+ + +L + + ++D RMSG A G V + Sbjct: 477 RDFVAVITFQ--GPRAN-GMPELHALTPALGILQDKGRHVALVTDGRMSG-ASGKVPAAI 532 Query: 492 HVAPEAAAGGPLGIVQDGDFIELDAYAGKLQLDISEEEMKRRLEARAKVLAERKPEMVGG 551 HV PE GGPLG V+DGD I LDA AG L+ + E R + + L+ + M Sbjct: 533 HVTPEVLGGGPLGRVRDGDIIRLDAEAGTLEAKVDATEWAARSQDSSD-LSGNQHGMGRE 591 Query: 552 YQSLYVDRVLQADEG 566 SL+ + V A+EG Sbjct: 592 LFSLFRNAVGTAEEG 606 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.136 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 2,012,232 Number of extensions: 93560 Number of successful extensions: 237 Number of sequences better than 1.0e-08: 5 Number of HSP's gapped: 221 Number of HSP's successfully gapped: 6 Length of query: 585 Length of database: 1,619,870 Length adjustment: 90 Effective length of query: 495 Effective length of database: 1,193,720 Effective search space: 590891400 Effective search space used: 590891400 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 133 (55.8 bits)