BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0521.p (261 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [H... 540 e-155 gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herb... 235 2e-63 gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum s... 117 9e-28 gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herba... 116 2e-27 gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein... 115 5e-27 >gi|300310374|ref|YP_003774466.1| dehydrogenase oxidoreductase [Herbaspirillum seropedicae SmR1] Length = 261 Score = 540 bits (1392), Expect = e-155, Method: Compositional matrix adjust. Identities = 261/261 (100%), Positives = 261/261 (100%) Query: 1 MSNTPQNVQLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCN 60 MSNTPQNVQLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCN Sbjct: 1 MSNTPQNVQLASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCN 60 Query: 61 EVAAAGHPKPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYW 120 EVAAAGHPKPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYW Sbjct: 61 EVAAAGHPKPLFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYW 120 Query: 121 NDRIAINQRPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTR 180 NDRIAINQRPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTR Sbjct: 121 NDRIAINQRPSFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTR 180 Query: 181 GLARDLGPHKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLF 240 GLARDLGPHKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLF Sbjct: 181 GLARDLGPHKIRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLF 240 Query: 241 LAADDSAMCTAQEFIVDAGWV 261 LAADDSAMCTAQEFIVDAGWV Sbjct: 241 LAADDSAMCTAQEFIVDAGWV 261 >gi|300313779|ref|YP_003777871.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 263 Score = 235 bits (600), Expect = 2e-63, Method: Compositional matrix adjust. Identities = 120/251 (47%), Positives = 159/251 (63%), Gaps = 1/251 (0%) Query: 11 ASFPSLKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKP 70 A + SL GKRV ITGGG+GIGAA+VEAF QGA V F+DIA E S+AL + A P Sbjct: 12 ALYRSLAGKRVVITGGGSGIGAALVEAFVGQGAQVCFLDIAAEPSQALVASLKDA-PIAP 70 Query: 71 LFRHCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQRP 130 F C+L ++ A +AT E++ +G D+L+NNAAND RHK +VT YW++R+A+N R Sbjct: 71 RFFPCNLMNLEALRATFTEIETVMGGVDILINNAANDDRHKTADVTPAYWDERLAVNLRH 130 Query: 131 SFFAVQSVVEGMKRRGGGSIINFSSISWHQSGGGFPVYTTAKASTLGLTRGLARDLGPHK 190 FF Q+V+ GM+ R G I+NF SISWH +Y TAKA G+T G+ARD G Sbjct: 131 QFFCAQAVLPGMRERKQGVILNFGSISWHLGLPDLTLYMTAKAGIEGMTHGMARDFGRDG 190 Query: 191 IRVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAMCT 250 +RVN + PG + T RQ LW E + I QCL + P +A + LFL++D A CT Sbjct: 191 VRVNAIIPGAIRTPRQTLLWHTPEEEAKILAAQCLPTRVDPHDVAALALFLSSDSGAKCT 250 Query: 251 AQEFIVDAGWV 261 +E+ VDAGW+ Sbjct: 251 GREYYVDAGWL 261 >gi|300310442|ref|YP_003774534.1| dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 117 bits (293), Expect = 9e-28, Method: Compositional matrix adjust. Identities = 86/250 (34%), Positives = 124/250 (49%), Gaps = 9/250 (3%) Query: 16 LKGKRVFITGGG--TGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKPLFR 73 LK K ITGG G+G A A+QGA V +D+A A + + + L Sbjct: 3 LKDKVAVITGGAGINGLGYATARLMAEQGARVVVLDLAQADPAAAAARIGS----QHLGL 58 Query: 74 HCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQRPSFF 133 D+ D + + A + Q G D+LVNNA Q K E+T ++ + ++ R + + Sbjct: 59 VADVTDKASCETAAAAILQQFGRIDILVNNAGITQPVKTLEITGADYDRILDVSLRGTLY 118 Query: 134 AVQSVVEGMKRRGGGSIINFSSISWHQSGG--GFPVYTTAKASTLGLTRGLARDLGPHKI 191 Q+V+ M+ + GSII SS+S + GG G P Y+ AKA LGL R +AR+ GP I Sbjct: 119 MSQAVLPAMRAQQNGSIICISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPEGI 178 Query: 192 RVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAMCTA 251 RVN++TPG + T+ IK L EE K IA L + +FL +D S CT Sbjct: 179 RVNSITPGLIGTD-IIKGKLTEEKKAEIAETIPLARLGRADDIGGACVFLGSDLSQYCTG 237 Query: 252 QEFIVDAGWV 261 V+ G + Sbjct: 238 ITLDVNGGML 247 >gi|300312612|ref|YP_003776704.1| 3-ketoacyl-CoA reductase [Herbaspirillum seropedicae SmR1] Length = 253 Score = 116 bits (290), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 93/250 (37%), Positives = 132/250 (52%), Gaps = 10/250 (4%) Query: 16 LKGKRVFITGGGTGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKPLFRHC 75 L GK VFI+G TGIGAA AFA +G+H+ ++E A K + Sbjct: 4 LAGKVVFISGASTGIGAAAARAFAARGSHLVVHYNSSEKEAEAVAAEVRAAGSKSITIGA 63 Query: 76 DLRDIPAFQATIAELQAQLGDFDVLVNNA-ANDQRHKLEEVTLEYWNDRIAINQRPSFFA 134 D+RD A A +A+ AQ+G DVL+NNA A +R LE VT E ++D I IN R Sbjct: 64 DVRDTSAINAAVAKAVAQMGRIDVLINNAGALVKRAPLETVTDELFDDIININARSVVAF 123 Query: 135 VQSVVEGMKRRGGGSIINFSSISWHQSGG-GFPVYTTAKASTLGLTRGLARDLGPHKIRV 193 ++V+ M+ +GGG+IIN +S++ GG G +Y +K LTRG+A++L KIRV Sbjct: 124 TRAVIPVMRAQGGGNIINVTSVAARHGGGPGALIYAASKGFVSTLTRGMAKELLADKIRV 183 Query: 194 NTVTPGWVMTERQIKLWLDE--EG-KKAIARNQCLQGDLLPWHLARMVLFLAADD-SAMC 249 N V PG +MT Q + E EG +K I + + P + L+LA++ S Sbjct: 184 NAVAPGVIMTPFQERFTTPEQLEGFRKTIPMGRIGE----PDECSGAFLYLASEQMSGYV 239 Query: 250 TAQEFIVDAG 259 T Q V+ G Sbjct: 240 TGQIIDVNGG 249 >gi|300309846|ref|YP_003773938.1| 3-oxoacyl-[acyl-carrier-protein] reductase [Herbaspirillum seropedicae SmR1] Length = 249 Score = 115 bits (287), Expect = 5e-27, Method: Compositional matrix adjust. Identities = 85/250 (34%), Positives = 125/250 (50%), Gaps = 9/250 (3%) Query: 16 LKGKRVFITGGG--TGIGAAIVEAFAQQGAHVAFVDIATEASEALCNEVAAAGHPKPLFR 73 LK K ITGG G+G + A QGA + +D+ + A + L Sbjct: 3 LKDKVAIITGGAGQNGLGFSTARLMAAQGARIVILDLERAEPAGAAARLGA----QHLGL 58 Query: 74 HCDLRDIPAFQATIAELQAQLGDFDVLVNNAANDQRHKLEEVTLEYWNDRIAINQRPSFF 133 D+ D A +A A + Q G D+LVNNA Q KL E+T + ++ + ++ R + + Sbjct: 59 VADVTDKAACEAAAAAVLKQFGRIDILVNNAGVTQPAKLLEITGKDYDRILDVSLRGTLY 118 Query: 134 AVQSVVEGMKRRGGGSIINFSSISWHQSGG--GFPVYTTAKASTLGLTRGLARDLGPHKI 191 Q+V+ M+ + GSI+ SS+S + GG G P Y+ AKA LGL R +AR+ GP I Sbjct: 119 MSQAVLPAMQAQQSGSIVCISSVSAQRGGGILGGPHYSAAKAGVLGLARAMAREFGPQGI 178 Query: 192 RVNTVTPGWVMTERQIKLWLDEEGKKAIARNQCLQGDLLPWHLARMVLFLAADDSAMCTA 251 RVN +TPG + T+ K L E K IA+ L P + + +FLA+D S CT Sbjct: 179 RVNCITPGLISTDIN-KDILSAEKKAEIAQTIPLGRLGGPDDVGGVCVFLASDLSQYCTG 237 Query: 252 QEFIVDAGWV 261 V+ G + Sbjct: 238 VTLDVNGGML 247 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.321 0.134 0.411 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 705,335 Number of extensions: 25200 Number of successful extensions: 189 Number of sequences better than 1.0e-08: 42 Number of HSP's gapped: 154 Number of HSP's successfully gapped: 42 Length of query: 261 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 178 Effective length of database: 1,226,865 Effective search space: 218381970 Effective search space used: 218381970 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 129 (54.3 bits)