BLASTP 2.2.18 [Mar-02-2008]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.

Query= 0020.0518.p
         (314 letters)

Database: BlastDatabases/689/689 
           4735 sequences; 1,619,870 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillu...   595   e-171
gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillu...   147   1e-36

>gi|300310377|ref|YP_003774469.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 292

 Score =  595 bits (1534), Expect = e-171,   Method: Compositional matrix adjust.
 Identities = 287/292 (98%), Positives = 289/292 (98%)

Query: 23  MNAQLLMDGHHELGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAF 82
           MN QLL+DGHHELGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAF
Sbjct: 1   MNVQLLVDGHHELGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAF 60

Query: 83  TADPQQLLIGLESRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNEDP 142
           TADPQQLLIGLESRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNED 
Sbjct: 61  TADPQQLLIGLESRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNEDA 120

Query: 143 GRAPIASFYRLNTDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGKVMVCDYDTASG 202
           GRAPIASFYRLNTDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGK+MVCDYDTASG
Sbjct: 121 GRAPIASFYRLNTDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGKIMVCDYDTASG 180

Query: 203 AVSGQRVFADVAQPGGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPTSQPSC 262
           AV GQRVFADVAQPGGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPTSQPSC
Sbjct: 181 AVGGQRVFADVAQPGGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPTSQPSC 240

Query: 263 VAFGGAQFDTLYVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPEVRFAG 314
           VAFGGAQFDTLYVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPEVRFAG
Sbjct: 241 VAFGGAQFDTLYVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPEVRFAG 292


>gi|300313173|ref|YP_003777265.1| gluconolactonase [Herbaspirillum
           seropedicae SmR1]
          Length = 302

 Score =  147 bits (370), Expect = 1e-36,   Method: Compositional matrix adjust.
 Identities = 93/285 (32%), Positives = 144/285 (50%), Gaps = 12/285 (4%)

Query: 35  LGEGVLWCDRSQSVFWTDIHASRLWNHDPQTGLTRSWGMPERLCCYAFTADPQQLLIGLE 94
           LGE  LW  +  +++W DI    +    P++G  + W +PE        A    L++ + 
Sbjct: 21  LGESPLWHAQEAALYWIDIPGKAVHRLHPESGQHKRWDLPEEPGSIVRHAQ-GGLVVAIR 79

Query: 95  SRLAFFNLSTGTIAPICRIEDDLPSTRLNDGRCDRQGRFVFGTLNEDPGRAPIASFYRLN 154
           + +   N  TG +  +     D    R NDGRCD  GRF  GT+ E P      + Y   
Sbjct: 80  TGMFHLNTDTGALTLLLAAPYDSSRIRFNDGRCDASGRFWCGTIYE-PRDRDGGTLYCFE 138

Query: 155 TDLTLERLSLPSIAISNSICFSLDGKLMYHCDSMAGKVMVCDYDTASGAVSGQRVF---- 210
            +    R +  ++  SN + FS D +LMYH ++ A ++ + DYD A+GA S  R+     
Sbjct: 139 RNAL--RDAHHAVVTSNGLAFSSDQRLMYHANTPAHRINLYDYDLATGATSNCRLLRQFD 196

Query: 211 ADVAQP---GGPDGSTIDAEGYLWNAQWGGARVVRYAPDGRIDRVVAIPTSQPSCVAFGG 267
           +D + P   G PDG+ +D++G  W A + G RV+R +P G I + + +P   P+ VAFGG
Sbjct: 197 SDKSAPDYGGRPDGAAVDSQGAYWCAMFEGGRVLRLSPQGEILQEIRLPARCPTMVAFGG 256

Query: 268 AQFDTLYVTTAHEGMSAEQRAADPQAGALFAVALADVRGLPEVRF 312
               TLY+TT   G S  + A  P++G + AV + DV G  E  +
Sbjct: 257 DDLRTLYITTGRNGRSPAELAQYPESGCVLAVRV-DVPGQLEYSY 300


  Database: BlastDatabases/689/689
    Posted date:  Apr 10, 2011 12:15 AM
  Number of letters in database: 1,619,870
  Number of sequences in database:  4735
  
Lambda     K      H
   0.321    0.137    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 4735
Number of Hits to DB: 1,097,489
Number of extensions: 48383
Number of successful extensions: 119
Number of sequences better than 1.0e-08: 2
Number of HSP's gapped: 119
Number of HSP's successfully gapped: 2
Length of query: 314
Length of database: 1,619,870
Length adjustment: 85
Effective length of query: 229
Effective length of database: 1,217,395
Effective search space: 278783455
Effective search space used: 278783455
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 130 (54.7 bits)