BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0020.0514.p (258 letters) Database: BlastDatabases/689/689 4735 sequences; 1,619,870 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogena... 529 e-151 gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herb... 106 2e-24 gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein... 106 2e-24 gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independe... 105 4e-24 gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reduc... 104 6e-24 >gi|300310380|ref|YP_003774472.1| short-chain alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 258 Score = 529 bits (1362), Expect = e-151, Method: Compositional matrix adjust. Identities = 256/258 (99%), Positives = 257/258 (99%) Query: 1 LDLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPEPQFWERLTAVQPRAALF 60 +DLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPE QFWERLTAVQPRAALF Sbjct: 1 MDLNLQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPESQFWERLTAVQPRAALF 60 Query: 61 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM 120 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM Sbjct: 61 QLELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGLEAGRDEFVASLERNLIHYYVM 120 Query: 121 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA Sbjct: 121 AHYCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNA 180 Query: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT Sbjct: 181 LIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTT 240 Query: 241 GQWIFVDGGYTHLDRALT 258 GQWIFVDGGYTHLDRALT Sbjct: 241 GQWIFVDGGYTHLDRALT 258 >gi|300313037|ref|YP_003777129.1| short-chain dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 369 Score = 106 bits (265), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 84/250 (33%), Positives = 127/250 (50%), Gaps = 13/250 (5%) Query: 5 LQDKVVIVTGGASGIGAAISLQLAAEGA-IPVVFARSAPEP-QFWERLTAVQPRAALFQL 62 L KV ++TGG SGIG A++L A EGA + +V+ + Q + + A+ L Sbjct: 123 LAGKVALITGGDSGIGRAVALAYAREGARLAIVYLDEEEDARQTQKDVQALDAPCLLLPG 182 Query: 63 ELQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGL--EAGRDEFVASLERNLIHYYVM 120 ++ CR AV ++ +GRLD LVNNA E + + + N+ + M Sbjct: 183 DVGSPEFCRSAVRAVMDAYGRLDVLVNNAAQQYPAQSIEEISQAQLEHTFRTNIFSMFYM 242 Query: 121 AHYCVPHLKATRGA-IINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVN 179 +P L TRGA IIN +S TA G + Y A+KGA ++ TR + L G+ VN Sbjct: 243 VKEAMPFL--TRGARIINTTSVTAYRGSKHLVDYAATKGAIVAFTRSLSQQLASAGIHVN 300 Query: 180 ALIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHT 239 A+ P + TPL A+F E++ + +PLG R +E+A VFL +E +S+ Sbjct: 301 AVAPGPIWTPLIP---ASFS--AEEVASFGKNVPLG-RPGQPDEVAPAYVFLATEGASYM 354 Query: 240 TGQWIFVDGG 249 TGQ + +GG Sbjct: 355 TGQVLHPNGG 364 >gi|300313110|ref|YP_003777202.1| 3-oxoacyl-(acyl-carrier-protein) reductase [Herbaspirillum seropedicae SmR1] Length = 256 Score = 106 bits (264), Expect = 2e-24, Method: Compositional matrix adjust. Identities = 78/246 (31%), Positives = 127/246 (51%), Gaps = 9/246 (3%) Query: 8 KVVIVTGGASGIGAAISLQLAAEG-AIPVVFARSAPEPQFWERLTAVQPRAALFQLELQD 66 +V +VTGGA GIGA+IS QLAA+G + V + + L A RAA Q++L Sbjct: 9 RVALVTGGAMGIGASISQQLAAKGHTVLVADINLDAASKTADALVAAGHRAAPLQMDLGS 68 Query: 67 DARCREAVAGTIERFGRLDGLVNNAGVNDSVG-LEAGRDEFVASLERNLIHYYVMA-HYC 124 A A + +GR D LVNNAGV + L+ + ++ ++ N+ + H Sbjct: 69 PESIAAAFAHIEKDYGRCDVLVNNAGVARTYSFLDYPLENWLQTMNVNVTGVLLAGQHAA 128 Query: 125 VPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALIPA 184 +K G I+N+SS + + + Y SK A + LTR+ A L G+ VN++ P Sbjct: 129 RLMVKQQWGRIVNISSISGIRAGAGRTAYGTSKAAVIGLTRQMAIELAQYGITVNSVAPG 188 Query: 185 EVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQWI 244 V TP+ + H ++ ++ +P+ +R+ T++E+A +L +E S++ TG I Sbjct: 189 PVDTPMTQAM-----HSEQTRESYYRLVPM-RRYGTTDEIASAVCYLAAEESAYITGHVI 242 Query: 245 FVDGGY 250 VDGG+ Sbjct: 243 PVDGGF 248 >gi|300311568|ref|YP_003775660.1| toluenesulfonate Zinc-independent alcohol dehydrogenase [Herbaspirillum seropedicae SmR1] Length = 252 Score = 105 bits (261), Expect = 4e-24, Method: Compositional matrix adjust. Identities = 85/251 (33%), Positives = 130/251 (51%), Gaps = 6/251 (2%) Query: 3 LNLQDKVVIVTGGASGIGAAISLQLAAEGA-IPVVFARSAPEPQFWERLTAVQPRAALFQ 61 + LQDKV IVTGG SG G I+ A EGA I V A + E + A RA + Sbjct: 1 MRLQDKVAIVTGGGSGFGEGIAKAYAREGAAIVVADIGEAGGLRVVEEIKAAGGRAVFAK 60 Query: 62 LELQDDARCREAVAGTIERFGRLDGLVNNAGVN--DSVGLEAGRDEFVASLERNLIHYYV 119 ++ A + +A +E FG+LD +VNNAG + LE DEF N+ ++ Sbjct: 61 ADVSKRADMDQLLATALEHFGKLDIVVNNAGTTHRNRPMLEVEEDEFDRVYAVNVKSIFL 120 Query: 120 MAHYCVPHLKATRG-AIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRV 178 A VP+ + G A IN++S + + + Y SKGA ++ ++ AA L D +RV Sbjct: 121 SAKTFVPYFRQVGGGAFINIASTAGIRPRPGLTWYNGSKGAVITTSKSMAAELGPDKIRV 180 Query: 179 NALIPAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSH 238 N + P T L +++ D P+ + + + IP+G RF+T E++A+ ++L S+ + Sbjct: 181 NCVNPVISATGLLSEFMGVPDTPENRKKFVAT-IPMG-RFSTPEDIANACLYLGSDEAEF 238 Query: 239 TTGQWIFVDGG 249 TG I VDGG Sbjct: 239 VTGVCIEVDGG 249 >gi|300311790|ref|YP_003775882.1| short-chain dehydrogenase/reductase family protein [Herbaspirillum seropedicae SmR1] Length = 261 Score = 104 bits (260), Expect = 6e-24, Method: Compositional matrix adjust. Identities = 86/247 (34%), Positives = 125/247 (50%), Gaps = 9/247 (3%) Query: 5 LQDKVVIVTGGASGIGAAISLQLAAEGAIPVVFARSAPEPQFWERLTAVQP-RAALFQLE 63 + KVVIVTG ASGIG A + + + EGA ++ R A + ++ P R A + + Sbjct: 12 FEGKVVIVTGAASGIGEATARRFSDEGARVLLADRDAA--ALGKVFDSLPPERTAARETD 69 Query: 64 LQDDARCREAVAGTIERFGRLDGLVNNAGVNDSVGL-EAGRDEFVASLERNLIHYYVMAH 122 + + R+ V IERFG+LD LV++AGV + E +++ N+ + A Sbjct: 70 VSHHEQVRQLVDFAIERFGQLDVLVSDAGVFAEGNVTEVSPEDWHRVQATNVNGVFYGAR 129 Query: 123 YCVPHLKATRGAIINVSSKTAVTGQGNTSGYCASKGAQLSLTREWAAALRDDGVRVNALI 182 +PHL+ TRG I+NV+S + + N S Y ASKGA +LTR A GVR+NA+ Sbjct: 130 EALPHLEKTRGCIVNVASVSGLAADWNLSAYNASKGAVCNLTRAMALDFGRKGVRINAVC 189 Query: 183 PAEVMTPLYEKWIATFDHPQEKLDAITSKIPLGKRFTTSEEMADMAVFLLSERSSHTTGQ 242 P+ T + D P LD +I LG R E+A + FL S +S G Sbjct: 190 PSLTHTAMTADMA---DDP-PLLDKFAERIALG-RGADPLEIAAVITFLASPDASFVNGV 244 Query: 243 WIFVDGG 249 + VDGG Sbjct: 245 NLPVDGG 251 Database: BlastDatabases/689/689 Posted date: Apr 10, 2011 12:15 AM Number of letters in database: 1,619,870 Number of sequences in database: 4735 Lambda K H 0.319 0.133 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 4735 Number of Hits to DB: 760,818 Number of extensions: 29862 Number of successful extensions: 214 Number of sequences better than 1.0e-08: 43 Number of HSP's gapped: 163 Number of HSP's successfully gapped: 43 Length of query: 258 Length of database: 1,619,870 Length adjustment: 83 Effective length of query: 175 Effective length of database: 1,226,865 Effective search space: 214701375 Effective search space used: 214701375 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)